(Circular) (Six-base) MAPSORT of: pFN21AB8321.seq Check: 8603 from: 1 to: 5581 pFN21AB8321 5581 bp With 182 enzymes: SgfI * February 27, 2009 14:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3391 278 Size: 53 83 186 936 1855 2468 Fragments arranged by size: 2468 1855 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2924 1937 Size: 987 4594 Acc65I G'GTAC_C Cuts at: 2902 2902 Size: 5581 AclI AA'CG_TT Cuts at: 1563 3708 4081 1563 Size: 2145 373 3063 Fragments arranged by size: 3063 2145 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2615 3644 5028 5296 1632 Size: 983 1029 1384 268 1917 Fragments arranged by size: 1917 1384 1029 983 268 AflII C'TTAA_G Cuts at: 820 1017 2618 820 Size: 197 1601 3783 Fragments arranged by size: 3783 1601 197 AflIII A'CryG_T Cuts at: 1203 2322 4480 5457 1203 Size: 1119 2158 977 1327 Fragments arranged by size: 2158 1327 1119 977 AgeI A'CCGG_T Cuts at: 4412 4412 Size: 5581 AhdI GACnn_n'nnGTC Cuts at: 4310 4310 Size: 5581 AleI CACnn'nnGTG Cuts at: 1166 2798 1166 Size: 1632 3949 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5581 AlwNI CAG_nnn'CTG Cuts at: 2197 3324 4896 2197 Size: 1127 1572 2882 Fragments arranged by size: 2882 1572 1127 ApaLI G'TGCA_C Cuts at: 3638 4794 3638 Size: 1156 4425 ApoI r'AATT_y Cuts at: 1493 2129 2162 2303 2543 2890 3116 3268 5377 Size: 636 33 141 240 347 226 152 2109 Cuts at: 5377 1493 Size: 1697 Fragments arranged by size: 2109 1697 636 347 240 226 152 141 33 AseI AT'TA_AT Cuts at: 160 1032 4135 160 Size: 872 3103 1606 Fragments arranged by size: 3103 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5581 AvaI C'yCGr_G Cuts at: 1870 1943 2906 1870 Size: 73 963 4545 Fragments arranged by size: 4545 963 73 BamHI G'GATC_C Cuts at: 2911 3278 2911 Size: 367 5214 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2902 4357 618 Size: 325 217 627 1115 1455 1842 Fragments arranged by size: 1842 1455 1115 627 325 217 BanII G_rGCy'C Cuts at: 1391 2900 1391 Size: 1509 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2621 928 Size: 971 722 3888 Fragments arranged by size: 3888 971 722 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3772 3806 1903 Size: 34 50 34 1751 34 3678 Fragments arranged by size: 3678 1751 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2277 3473 4683 2277 Size: 1196 1210 3175 Fragments arranged by size: 3175 1210 1196 BclI T'GATC_A Cuts at: 1548 2644 1548 Size: 1096 4485 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4192 136 Size: 107 122 71 631 605 2520 1525 Fragments arranged by size: 2520 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2344 5576 2344 Size: 3232 2349 BlpI GC'TnA_GC Cuts at: 2998 2998 Size: 5581 Bme1580I G_kGCm'C Cuts at: 1790 3642 4798 1790 Size: 1852 1156 2573 Fragments arranged by size: 2573 1852 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5581 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3312 4270 442 Size: 416 53 83 399 24 1895 958 1753 Fragments arranged by size: 1895 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5581 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2459 2459 Size: 5581 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4241 1239 Size: 3002 2579 Bpu10I CC'TnA_GC Cuts at: 2788 2788 Size: 5581 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3706 4571 4869 3706 Size: 865 298 4418 Fragments arranged by size: 4418 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4244 882 Size: 617 2745 2219 Fragments arranged by size: 2745 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3372 493 Size: 711 2168 2702 Fragments arranged by size: 2702 2168 711 BsaBI GATnn'nnATC Cuts at: 2849 5575 2849 Size: 2726 2855 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3388 3770 4405 275 Size: 53 83 186 936 1855 382 635 1451 Fragments arranged by size: 1855 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4014 4412 4686 4833 5244 4014 Size: 398 274 147 411 4351 Fragments arranged by size: 4351 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2312 2342 1178 Size: 30 165 30 909 30 4417 Fragments arranged by size: 4417 909 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5581 BseYI C'CCAG_C Cuts at: 2233 4784 5280 2233 Size: 2551 496 2534 Fragments arranged by size: 2551 2534 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2390 1163 Size: 573 654 4354 Fragments arranged by size: 4354 654 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3031 3792 4820 1540 Size: 275 186 1030 761 1028 2301 Fragments arranged by size: 2301 1030 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2100 3642 3727 4798 2100 Size: 1542 85 1071 2883 Fragments arranged by size: 2883 1542 1071 85 BsmI GAATG_Cn' Cuts at: 3092 3185 3092 Size: 93 5488 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2100 2900 3642 3727 4798 1391 Size: 399 310 800 742 85 1071 2174 Fragments arranged by size: 2174 1071 800 742 399 310 85 BspEI T'CCGG_A Cuts at: 5244 5244 Size: 5581 BspHI T'CATG_A Cuts at: 3470 5200 3470 Size: 1730 3851 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2869 2936 844 Size: 2025 67 3489 Fragments arranged by size: 3489 2025 67 BsrBI CCG'CTC Cuts at: 1394 1823 3468 1394 Size: 429 1645 3507 Fragments arranged by size: 3507 1645 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2212 4076 4250 65 Size: 1183 964 1864 174 1396 Fragments arranged by size: 1864 1396 1183 964 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4225 4412 1700 Size: 110 2415 187 2869 Fragments arranged by size: 2869 2415 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5581 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5581 BssSI C'ACGA_G Cuts at: 3641 4653 3641 Size: 1012 4569 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5581 BstBI TT'CG_AA Cuts at: 4385 4385 Size: 5581 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5581 BstYI r'GATC_y Cuts at: 1972 2082 2344 2911 3278 3663 3680 5132 5218 Size: 110 262 567 367 385 17 1452 86 Cuts at: 5218 5230 5576 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 567 385 367 346 262 110 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2118 513 Size: 552 1053 3976 Fragments arranged by size: 3976 1053 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4842 Fragments arranged by size: 4842 683 56 BtsI GCAGTG_nn' Cuts at: 2402 2696 3097 3891 3911 2402 Size: 294 401 794 20 4072 Fragments arranged by size: 4072 794 401 294 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5581 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2752 2787 529 Size: 35 2188 35 3323 Fragments arranged by size: 3323 2188 35 35 DraI TTT'AAA Cuts at: 2220 2885 2220 Size: 665 4916 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4588 809 Size: 507 3272 1802 Fragments arranged by size: 3272 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3028 3917 5546 Size: 54 1425 50 275 18 1198 889 1629 Cuts at: 5546 8 Size: 43 Fragments arranged by size: 1629 1425 1198 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3028 1537 Size: 275 1216 4090 Fragments arranged by size: 4090 1216 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2064 2379 2526 2597 3511 1353 Size: 711 315 147 71 914 3423 Fragments arranged by size: 3423 914 711 315 147 71 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4168 4542 4688 387 Size: 1064 138 2579 374 146 1280 Fragments arranged by size: 2579 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2615 3644 4241 5028 5296 1239 Size: 393 983 1029 597 787 268 1524 Fragments arranged by size: 1524 1029 983 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2315 2315 Size: 5581 EcoRI G'AATT_C Cuts at: 2543 2890 3268 2543 Size: 347 378 4856 Fragments arranged by size: 4856 378 347 FspI TGC'GCA Cuts at: 4087 4087 Size: 5581 HaeII r_GCGC'y Cuts at: 1678 4728 1678 Size: 3050 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2312 2344 2813 2845 4222 Size: 32 135 32 740 32 469 32 1377 Cuts at: 4222 4254 4296 4328 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1377 740 469 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2212 2925 3171 669 Size: 988 281 274 713 246 3079 Fragments arranged by size: 3079 988 713 281 274 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5581 HpaI GTT'AAC Cuts at: 3171 3171 Size: 5581 KpnI G_GTAC'C Cuts at: 2906 2906 Size: 5581 MfeI C'AATT_G Cuts at: 2154 3180 2154 Size: 1026 4555 MluI A'CGCG_T Cuts at: 5457 5457 Size: 5581 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2631 4695 4879 1305 Size: 1326 2064 184 2007 Fragments arranged by size: 2064 2007 1326 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3759 Fragments arranged by size: 3759 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2798 3539 3898 4057 518 Size: 648 1632 741 359 159 2042 Fragments arranged by size: 2042 1632 741 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2233 2997 3674 4822 5067 1606 Size: 627 764 677 1148 245 2120 Fragments arranged by size: 2120 1148 764 677 627 245 NcoI C'CATG_G Cuts at: 513 1065 2118 513 Size: 552 1053 3976 Fragments arranged by size: 3976 1053 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5581 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5581 NotI GC'GGCC_GC Cuts at: 3028 3028 Size: 5581 NspI r_CATG'y Cuts at: 1132 1229 2326 2875 2939 4484 1132 Size: 97 1097 549 64 1545 2229 Fragments arranged by size: 2229 1545 1097 549 97 64 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5581 PciI A'CATG_T Cuts at: 2322 4480 2322 Size: 2158 3423 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5581 PmeI GTTT'AAAC Cuts at: 2885 2885 Size: 5581 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3650 3682 5163 5195 3650 Size: 32 1481 32 4036 Fragments arranged by size: 4036 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5581 PsiI TTA'TAA Cuts at: 3151 3151 Size: 5581 PstI C_TGCA'G Cuts at: 830 2014 2933 830 Size: 1184 919 3478 Fragments arranged by size: 3478 1184 919 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5581 PvuII CAG'CTG Cuts at: 2233 2233 Size: 5581 SalI G'TCGA_C Cuts at: 1936 2923 1936 Size: 987 4594 SapI GCTCTTCn'nnn_ Cuts at: 2597 2597 Size: 5581 SbfI CC_TGCA'GG Cuts at: 2933 2933 Size: 5581 ScaI AGT'ACT Cuts at: 2768 3829 5541 2768 Size: 1061 1712 2808 Fragments arranged by size: 2808 1712 1061 SfcI C'TryA_G Cuts at: 826 1045 2010 2366 2929 4064 4745 4936 826 Size: 219 965 356 563 1135 681 191 1471 Fragments arranged by size: 1471 1135 965 681 563 356 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5581 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5581 SmaI CCC'GGG Cuts at: 1872 2908 1872 Size: 1036 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2618 3685 4586 4848 820 Size: 197 926 675 1067 901 262 1553 Fragments arranged by size: 1553 1067 926 901 675 262 197 SnaBI TAC'GTA Cuts at: 493 3372 493 Size: 2879 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5581 SphI G_CATG'C Cuts at: 1132 2939 1132 Size: 1807 3774 SspI AAT'ATT Cuts at: 5 52 3505 5 Size: 47 3453 2081 Fragments arranged by size: 3453 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5581 StyI C'CwwG_G Cuts at: 513 1065 2118 3020 513 Size: 552 1053 902 3074 Fragments arranged by size: 3074 1053 902 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3608 3625 3778 3963 4408 325 Size: 1255 2028 17 153 185 445 1498 Fragments arranged by size: 2028 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2325 2766 3827 Size: 274 80 33 51 1443 348 441 1061 Cuts at: 3827 5539 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1061 441 348 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2116 2148 5541 5573 2116 Size: 32 3393 32 2124 Fragments arranged by size: 3393 2124 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2453 1316 Size: 1137 4444 XbaI T'CTAG_A Cuts at: 2062 2917 2062 Size: 855 4726 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5581 XmaI C'CCGG_G Cuts at: 1870 2906 1870 Size: 1036 4545 XmnI GAAnn'nnTTC Cuts at: 2547 2564 3710 2547 Size: 17 1146 4418 Fragments arranged by size: 4418 1146 17 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3389 276 Size: 53 83 186 936 1855 2468 Fragments arranged by size: 2468 1855 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI