################################## # NO CUT ENZYMES ( 203 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AciI AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BfaI BfrI BglI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsiYI BslI BsmAI BsmBI BsoBI Bsp1407I BspDI BspEI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DpnII DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRII EcoRV EcoT22I FauI FbaI FokI FseI FspI HaeII HaeIII HgaI HhaI Hin1I HinP1I HincII HindII HpaI HphI KasI KpnI Ksp632I KspI MaeI MaeII MamI MboI MflI MluI MluNI MroI MscI MvaI MvnI NaeI NarI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI RsaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SexAI SfaNI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tsp45I Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AspHI 351 GAGCAC G[AT]GC[AT]^C AvaII 301 GGTCC G^G[AT]CC BcnI 304 CCCGG CC^[GC]GG BmyI 351 GAGCAC G[AGT]GC[ACT]^C BpmI 356 CTCCAG CTGGAG(16/14) BsaJI 207 CCTTGG C^C[ACGT][ACGT]GG BsiHKAI 351 GAGCAC G[AT]GC[AT]^C BsmFI 302 GTCCC GGGAC(10/14) BsmI 240 GAATGC GAATGC(1/-1) Bsp1286I 351 GAGCAC G[AGT]GC[ACT]^C BspHI 266 TCATGA T^CATGA BssKI 304 CCCGG ^CC[ACGT]GG Cfr13I 301 GGTCC G^G[ACGT]CC DraII 300 GGGTCCC [GA]G^G[ACGT]CC[CT] EcoO109I 300 GGGTCCC [GA]G^G[ACGT]CC[CT] EcoRI 212 GAATTC G^AATTC EcoT14I 207 CCTTGG C^C[AT][AT]GG HapII 305 CCGG C^CGG HindIII 181 AAGCTT A^AGCTT HpaII 305 CCGG C^CGG MaeIII 39 GTTAC ^GT[ACGT]AC MboII 81 TCTTC GAAGA(8/7) MfeI 128 CAATTG C^AATTG MnlI 377 GAGG CCTC(7/6) MslI 267 CATGACGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 305 CCGG C^CGG MunI 128 CAATTG C^AATTG NciI 304 CCCGG CC^[GC]GG PleI 55 GACTC GAGTC(4/5) PpuMI 300 GGGTCCC [GA]G^G[AT]CC[CT] RcaI 266 TCATGA T^CATGA Sau96I 301 GGTCC G^G[ACGT]CC ScrFI 304 CCCGG CC^[ACGT]GG SfcI 155 CTATAG C^T[GA][CT]AG StyI 207 CCTTGG C^C[AT][AT]GG TspRI 231 CAGTG CA[GC]TG[ACGT][ACGT]^ ################################### # TWO CUT ENZYMES ( 12 enzymes) # ################################### BbvI 277 GCAGC GCAGC(8/12) 383 GCTGC BsrI 230 CCAGT ACTGG(1/-1) 358 CCAGT Fnu4HI 277 GCAGC GC^[ACGT]GC 383 GCTGC ItaI 277 GCAGC GC^[ACGT]GC 383 GCTGC MseI 287 TTAA T^TAA 341 TTAA MspA1I 278 CAGCTG C[AC]G^C[GT]G 314 CAGCTG NlaIII 9 CATG CATG^ 267 CATG NlaIV 300 GGGTCC GG[ACGT]^[ACGT]CC 301 GGTCCC PvuII 278 CAGCTG CAG^CTG 314 CAGCTG TfiI 12 GAATC G^A[AT]TC 202 GATTC Tru9I 287 TTAA T^TAA 341 TTAA TseI 277 GCAGC G^C[AT]GC 383 GCTGC ######################################### # 3 OR MORE CUT ENZYMES ( 7 enzymes) # ######################################### AcsI AluI ApoI DdeI HinfI MwoI Tsp509I