Restriction map for
pFN21AB8319
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# NO CUT ENZYMES ( 152 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflIII AgeI AhdI
Alw44I AlwNI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BglI BglII
BlnI BlpI BpmI Bpu1102I BsaAI
BsaHI BsaI BsaWI BseAI BseRI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspLU11I BsrBI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I CelII CfoI
ClaI CpoI DraI DraII DraIII
DrdI DsaI EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I KasI KpnI Ksp632I
KspI MaeII MfeI MluI MroI
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI TfiI Tsp509I
Tth111I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccII 121 CGCG CG^CG
AfaI 379 GTAC GT^AC
AflII 481 CTTAAG C^TTAAG
AocI 370 CCTGAGG CC^T[ACGT]AGG
AspHI 759 GAGCTC G[AT]GC[AT]^C
BalI 24 TGGCCA TGG^CCA
BcnI 212 CCCGG CC^[GC]GG
BfaI 473 CTAG C^TAG
BfrI 481 CTTAAG C^TTAAG
BsaBI 877 GATAGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiHKAI 759 GAGCTC G[AT]GC[AT]^C
BsmAI 259 GAGAC GTCTC(1/5)
BsmFI 163 GTCCC GGGAC(10/14)
BspHI 405 TCATGA T^CATGA
BsrFI 106 ACCGGC [GA]^CCGG[CT]
BstAPI 144 GCAGCTGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 121 CGCG CG^CG
BstXI 566 CCAACGATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 370 CCTGAGG CC^T[ACGT]AGG
Cfr10I 106 ACCGGC [GA]^CCGG[CT]
EaeI 24 TGGCCA [CT]^GGCC[GA]
Eco81I 370 CCTGAGG CC^T[ACGT]AGG
HincII 102 GTTAAC GT[CT]^[GA]AC
HindII 102 GTTAAC GT[CT]^[GA]AC
HpaI 102 GTTAAC GTT^AAC
HphI 494 TCACC GGTGA(8/7)
MaeI 473 CTAG C^TAG
MaeIII 413 GTGAC ^GT[ACGT]AC
MamI 877 GATAGCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 24 TGGCCA TGG^CCA
MscI 24 TGGCCA TGG^CCA
MslI 406 CATGAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 121 CGCG CG^CG
NciI 212 CCCGG CC^[GC]GG
PstI 349 CTGCAG CTGCA^G
RcaI 405 TCATGA T^CATGA
RsaI 379 GTAC GT^AC
SacI 759 GAGCTC GAGCT^C
SmlI 481 CTTAAG C^T[CT][GA]AG
StuI 692 AGGCCT AGG^CCT
TaqI 390 TCGA T^CGA
Tsp45I 413 GTGAC ^GT[GC]AC
TthHB8I 390 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AvaII 531 GGACC G^G[AT]CC
554 GGACC
BamHI 215 GGATCC G^GATCC
618 GGATCC
BanII 358 GAGCCC G[GA]GC[CT]^C
759 GAGCTC
BbsI 288 GAAGAC GAAGAC(2/6)
504 GTCTTC
BmyI 358 GAGCCC G[AGT]GC[ACT]^C
759 GAGCTC
BpuAI 288 GAAGAC GAAGAC(2/6)
504 GTCTTC
Bsp1286I 358 GAGCCC G[AGT]GC[ACT]^C
759 GAGCTC
BspMI 419 ACCTGC ACCTGC(4/8)
522 ACCTGC
BsrDI 110 GCAATG GCAATG(2/0)
153 GCAATG
EcoT14I 399 CCTTGG C^C[AT][AT]GG
831 CCTTGG
FauI 354 GCGGG CCCGC(4/6)
657 GCGGG
HapII 107 CCGG C^CGG
213 CCGG
HindIII 91 AAGCTT A^AGCTT
484 AAGCTT
HinfI 318 GACTC G^A[ACGT]TC
601 GACTC
HpaII 107 CCGG C^CGG
213 CCGG
MspI 107 CCGG C^CGG
213 CCGG
NlaIII 406 CATG CATG^
588 CATG
PflMI 27 CCAGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
545 CCAAGCTGTGG
PleI 318 GACTC GAGTC(4/5)
601 GACTC
SfcI 266 CTGTAG C^T[GA][CT]AG
349 CTGCAG
StyI 399 CCTTGG C^C[AT][AT]GG
831 CCTTGG
Van91I 27 CCAGTTTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
545 CCAAGCTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AluI AlwI BbvI BsaJI
BsgI BsiYI BslI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII EcoRII Fnu4HI FokI
HaeIII ItaI MboI MboII MflI
MnlI MseI MspA1I MvaI MwoI
NdeII NlaIV PvuII Sau3AI Sau96I
ScrFI SfaNI Tru9I TseI TspRI
XhoII