(Circular) (Six-base) MAPSORT of: pFN21AB8319.seq Check: 867 from: 1 to: 5602 pFN21AB8319 5602 bp With 182 enzymes: SgfI * February 27, 2009 14:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3412 278 Size: 53 83 186 936 1876 2468 Fragments arranged by size: 2468 1876 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2945 1937 Size: 1008 4594 Acc65I G'GTAC_C Cuts at: 2923 2923 Size: 5602 AclI AA'CG_TT Cuts at: 1563 3729 4102 1563 Size: 2166 373 3063 Fragments arranged by size: 3063 2166 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2270 2502 3665 5049 5317 1632 Size: 638 232 1163 1384 268 1917 Fragments arranged by size: 1917 1384 1163 638 268 232 AflII C'TTAA_G Cuts at: 820 1017 2486 820 Size: 197 1469 3936 Fragments arranged by size: 3936 1469 197 AflIII A'CryG_T Cuts at: 1203 4501 5478 1203 Size: 3298 977 1327 Fragments arranged by size: 3298 1327 977 AgeI A'CCGG_T Cuts at: 4433 4433 Size: 5602 AhdI GACnn_n'nnGTC Cuts at: 4331 4331 Size: 5602 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5602 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2201 1184 Size: 1017 4585 AlwNI CAG_nnn'CTG Cuts at: 3345 4917 3345 Size: 1572 4030 ApaLI G'TGCA_C Cuts at: 3659 4815 3659 Size: 1156 4446 ApoI r'AATT_y Cuts at: 1493 2911 3137 3289 5398 1493 Size: 1418 226 152 2109 1697 Fragments arranged by size: 2109 1697 1418 226 152 AseI AT'TA_AT Cuts at: 160 1032 4156 160 Size: 872 3124 1606 Fragments arranged by size: 3124 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5602 AvaI C'yCGr_G Cuts at: 1870 1943 2927 1870 Size: 73 984 4545 Fragments arranged by size: 4545 984 73 BamHI G'GATC_C Cuts at: 2220 2623 2932 3299 2220 Size: 403 309 367 4523 Fragments arranged by size: 4523 403 367 309 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2923 4378 618 Size: 325 217 627 1136 1455 1842 Fragments arranged by size: 1842 1455 1136 627 325 217 BanII G_rGCy'C Cuts at: 1391 2367 2768 2921 1391 Size: 976 401 153 4072 Fragments arranged by size: 4072 976 401 153 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2300 2502 928 Size: 971 401 202 4028 Fragments arranged by size: 4028 971 401 202 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3793 3827 1903 Size: 34 1856 34 3678 Fragments arranged by size: 3678 1856 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3494 4704 3494 Size: 1210 4392 BclI T'GATC_A Cuts at: 1548 1548 Size: 5602 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4213 136 Size: 107 122 71 631 605 2541 1525 Fragments arranged by size: 2541 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5597 5597 Size: 5602 BlpI GC'TnA_GC Cuts at: 3019 3019 Size: 5602 Bme1580I G_kGCm'C Cuts at: 1790 3663 4819 1790 Size: 1873 1156 2573 Fragments arranged by size: 2573 1873 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5602 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2060 3333 4291 Size: 416 53 83 399 24 643 1273 958 Cuts at: 4291 442 Size: 1753 Fragments arranged by size: 1753 1273 958 643 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5602 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4262 1239 Size: 3023 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3727 4592 4890 3727 Size: 865 298 4439 Fragments arranged by size: 4439 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4265 882 Size: 617 2766 2219 Fragments arranged by size: 2766 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3393 493 Size: 711 2189 2702 Fragments arranged by size: 2702 2189 711 BsaBI GATnn'nnATC Cuts at: 2886 5596 2886 Size: 2710 2892 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3409 3791 4426 275 Size: 53 83 186 936 1876 382 635 1451 Fragments arranged by size: 1876 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4035 4433 4707 4854 5265 4035 Size: 398 274 147 411 4372 Fragments arranged by size: 4372 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5377 Fragments arranged by size: 5377 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5602 BseYI C'CCAG_C Cuts at: 4805 5301 4805 Size: 496 5106 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2171 2695 2812 1163 Size: 573 435 524 117 3953 Fragments arranged by size: 3953 573 524 435 117 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3052 3813 4841 1540 Size: 275 186 1051 761 1028 2301 Fragments arranged by size: 2301 1051 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2768 3663 3748 4819 2768 Size: 895 85 1071 3551 Fragments arranged by size: 3551 1071 895 85 BsmI GAATG_Cn' Cuts at: 3113 3206 3113 Size: 93 5509 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2367 2768 2921 3663 3748 4819 1391 Size: 399 577 401 153 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 577 401 399 153 85 BspEI T'CCGG_A Cuts at: 5265 5265 Size: 5602 BspHI T'CATG_A Cuts at: 2410 3491 5221 2410 Size: 1081 1730 2791 Fragments arranged by size: 2791 1730 1081 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2433 2536 2957 844 Size: 1589 103 421 3489 Fragments arranged by size: 3489 1589 421 103 BsrBI CCG'CTC Cuts at: 1394 1823 3489 1394 Size: 429 1666 3507 Fragments arranged by size: 3507 1666 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2122 2165 4097 4271 65 Size: 1183 874 43 1932 174 1396 Fragments arranged by size: 1932 1396 1183 874 174 43 BsrFI r'CCGG_y Cuts at: 1700 1810 2111 4246 4433 1700 Size: 110 301 2135 187 2869 Fragments arranged by size: 2869 2135 301 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5602 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5602 BssSI C'ACGA_G Cuts at: 3662 4674 3662 Size: 1012 4590 BstAPI GCAn_nnn'nTGC Cuts at: 794 2155 794 Size: 1361 4241 BstBI TT'CG_AA Cuts at: 4406 4406 Size: 5602 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5602 BstXI CCAn_nnnn'nTGG Cuts at: 2578 2578 Size: 5602 BstYI r'GATC_y Cuts at: 1972 2019 2220 2596 2623 2647 2932 3299 3684 Size: 47 201 376 27 24 285 367 385 Cuts at: 3684 3701 5153 5239 5251 5597 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 385 376 367 346 285 201 86 47 27 24 17 12 Bsu36I CC'TnA_GG Cuts at: 2376 2376 Size: 5602 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4111 Fragments arranged by size: 4111 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2820 487 Size: 683 56 1594 3269 Fragments arranged by size: 3269 1594 683 56 BtsI GCAGTG_nn' Cuts at: 2049 3118 3912 3932 2049 Size: 1069 794 20 3719 Fragments arranged by size: 3719 1069 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5602 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5567 DraI TTT'AAA Cuts at: 2906 2906 Size: 5602 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4609 809 Size: 507 3293 1802 Fragments arranged by size: 3293 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2029 3049 3938 Size: 54 1425 50 275 18 199 1020 889 Cuts at: 3938 5567 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1020 889 275 199 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3049 1537 Size: 275 1237 4090 Fragments arranged by size: 4090 1237 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3532 1353 Size: 2179 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4189 4563 4709 387 Size: 1064 138 2600 374 146 1280 Fragments arranged by size: 2600 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2766 2766 Size: 5602 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2270 2502 3665 4262 5049 5317 1239 Size: 393 638 232 1163 597 787 268 1524 Fragments arranged by size: 1524 1163 787 638 597 393 268 232 EcoRI G'AATT_C Cuts at: 2911 3289 2911 Size: 378 5224 FspI TGC'GCA Cuts at: 4108 4108 Size: 5602 HaeII r_GCGC'y Cuts at: 1678 4749 1678 Size: 3071 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2045 2077 4243 4275 4317 Size: 32 135 32 473 32 2166 32 42 Cuts at: 4317 4349 1373 Size: 32 2626 Fragments arranged by size: 2626 2166 473 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2109 2946 3192 669 Size: 988 281 171 837 246 3079 Fragments arranged by size: 3079 988 837 281 246 171 HindIII A'AGCT_T Cuts at: 748 2096 2489 748 Size: 1348 393 3861 Fragments arranged by size: 3861 1348 393 HpaI GTT'AAC Cuts at: 2109 3192 2109 Size: 1083 4519 KpnI G_GTAC'C Cuts at: 2927 2927 Size: 5602 MfeI C'AATT_G Cuts at: 3201 3201 Size: 5602 MluI A'CGCG_T Cuts at: 5478 5478 Size: 5602 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4716 4900 1305 Size: 3411 184 2007 Fragments arranged by size: 3411 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2031 10 Size: 54 1425 343 199 3581 Fragments arranged by size: 3581 1425 343 199 54 MslI CAynn'nnrTG Cuts at: 518 1166 2415 3560 3919 4078 518 Size: 648 1249 1145 359 159 2042 Fragments arranged by size: 2042 1249 1145 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2152 2270 2359 2817 2826 3018 3695 4843 Size: 546 118 89 458 9 192 677 1148 Cuts at: 4843 5088 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 546 458 245 192 118 89 9 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4111 Fragments arranged by size: 4111 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5602 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5602 NotI GC'GGCC_GC Cuts at: 3049 3049 Size: 5602 NspI r_CATG'y Cuts at: 1132 1229 2960 4505 1132 Size: 97 1731 1545 2229 Fragments arranged by size: 2229 1731 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5602 PciI A'CATG_T Cuts at: 4501 4501 Size: 5602 PflMI CCAn_nnn'nTGG Cuts at: 1108 2038 2556 1108 Size: 930 518 4154 Fragments arranged by size: 4154 930 518 PmeI GTTT'AAAC Cuts at: 2906 2906 Size: 5602 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3671 3703 5184 5216 3671 Size: 32 1481 32 4057 Fragments arranged by size: 4057 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5602 PsiI TTA'TAA Cuts at: 3172 3172 Size: 5602 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2376 2408 2376 Size: 32 5570 PstI C_TGCA'G Cuts at: 830 2358 2954 830 Size: 1528 596 3478 Fragments arranged by size: 3478 1528 596 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5602 PvuII CAG'CTG Cuts at: 2152 2270 2826 2152 Size: 118 556 4928 Fragments arranged by size: 4928 556 118 SacI G_AGCT'C Cuts at: 2768 2768 Size: 5602 SalI G'TCGA_C Cuts at: 1936 2944 1936 Size: 1008 4594 SbfI CC_TGCA'GG Cuts at: 2954 2954 Size: 5602 ScaI AGT'ACT Cuts at: 3850 5562 3850 Size: 1712 3890 SfcI C'TryA_G Cuts at: 826 1045 2271 2354 2950 4085 4766 4957 826 Size: 219 1226 83 596 1135 681 191 1471 Fragments arranged by size: 1471 1226 1135 681 596 219 191 83 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5602 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5602 SmaI CCC'GGG Cuts at: 1872 2929 1872 Size: 1057 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2486 3706 4607 4869 820 Size: 197 926 543 1220 901 262 1553 Fragments arranged by size: 1553 1220 926 901 543 262 197 SnaBI TAC'GTA Cuts at: 493 3393 493 Size: 2900 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5602 SphI G_CATG'C Cuts at: 1132 2960 1132 Size: 1828 3774 SspI AAT'ATT Cuts at: 5 52 3526 5 Size: 47 3474 2081 Fragments arranged by size: 3474 2081 47 StuI AGG'CCT Cuts at: 1517 2699 1517 Size: 1182 4420 StyI C'CwwG_G Cuts at: 513 1065 2004 2404 2836 3041 513 Size: 552 939 400 432 205 3074 Fragments arranged by size: 3074 939 552 432 400 205 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3629 3646 3799 3984 4429 325 Size: 1255 2049 17 153 185 445 1498 Fragments arranged by size: 2049 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2383 3848 5560 Size: 274 80 33 51 1443 406 1465 1712 Cuts at: 5560 96 Size: 138 Fragments arranged by size: 1712 1465 1443 406 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5562 5594 5562 Size: 32 5570 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5602 XbaI T'CTAG_A Cuts at: 2938 2938 Size: 5602 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5602 XmaI C'CCGG_G Cuts at: 1870 2927 1870 Size: 1057 4545 XmnI GAAnn'nnTTC Cuts at: 3731 3731 Size: 5602 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3410 276 Size: 53 83 186 936 1876 2468 Fragments arranged by size: 2468 1876 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PsrI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI