Restriction map for
pFN21AB8319
(Linear) (Six-base) MAPSORT of: KIBB8319.seq Check: 6870 from: 1 to: 897
KIBB8319.orf
With 182 enzymes: SgfI *
March 3, 2008 11:04 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 497 897
Size: 265 232 400
Fragments arranged by size:
400 265 232
AflII C'TTAA_G
Cuts at: 0 481 897
Size: 481 416
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 196 897
Size: 196 701
BamHI G'GATC_C
Cuts at: 0 215 618 897
Size: 215 403 279
Fragments arranged by size:
403 279 215
BanII G_rGCy'C
Cuts at: 0 362 763 897
Size: 362 401 134
Fragments arranged by size:
401 362 134
BbsI GAAGACnn'nnnn_
Cuts at: 0 295 497 897
Size: 295 202 400
Fragments arranged by size:
400 295 202
BmrI ACTGGGnnnn_n'
Cuts at: 0 55 897
Size: 55 842
BsaBI GATnn'nnATC
Cuts at: 0 881 897
Size: 881 16
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 166 690 807 897
Size: 166 524 117 90
Fragments arranged by size:
524 166 117 90
BsiHKAI G_wGCw'C
Cuts at: 0 763 897
Size: 763 134
Bsp1286I G_dGCh'C
Cuts at: 0 362 763 897
Size: 362 401 134
Fragments arranged by size:
401 362 134
BspHI T'CATG_A
Cuts at: 0 405 897
Size: 405 492
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 428 531 897
Size: 428 103 366
Fragments arranged by size:
428 366 103
BsrDI GCAATG_nn'
Cuts at: 0 117 160 897
Size: 117 43 737
Fragments arranged by size:
737 117 43
BsrFI r'CCGG_y
Cuts at: 0 106 897
Size: 106 791
BstAPI GCAn_nnn'nTGC
Cuts at: 0 150 897
Size: 150 747
BstXI CCAn_nnnn'nTGG
Cuts at: 0 573 897
Size: 573 324
BstYI r'GATC_y
Cuts at: 0 14 215 591 618 642 897
Size: 14 201 376 27 24 255
Fragments arranged by size:
376 255 201 27 24 14
Bsu36I CC'TnA_GG
Cuts at: 0 371 897
Size: 371 526
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 815 897
Size: 815 82
BtsI GCAGTG_nn'
Cuts at: 0 44 897
Size: 44 853
EaeI y'GGCC_r
Cuts at: 0 24 897
Size: 24 873
EcoICRI GAG'CTC
Cuts at: 0 761 897
Size: 761 136
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 265 497 897
Size: 265 232 400
Fragments arranged by size:
400 265 232
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 40 72 897
Size: 40 32 825
Fragments arranged by size:
825 40 32
HincII GTy'rAC
Cuts at: 0 104 897
Size: 104 793
HindIII A'AGCT_T
Cuts at: 0 91 484 897
Size: 91 393 413
Fragments arranged by size:
413 393 91
HpaI GTT'AAC
Cuts at: 0 104 897
Size: 104 793
MscI TGG'CCA
Cuts at: 0 26 897
Size: 26 871
MslI CAynn'nnrTG
Cuts at: 0 410 897
Size: 410 487
MspA1I CmG'CkG
Cuts at: 0 147 265 354 812 821 897
Size: 147 118 89 458 9 76
Fragments arranged by size:
458 147 118 89 76 9
PflMI CCAn_nnn'nTGG
Cuts at: 0 33 551 897
Size: 33 518 346
Fragments arranged by size:
518 346 33
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 371 403 897
Size: 371 32 494
Fragments arranged by size:
494 371 32
PstI C_TGCA'G
Cuts at: 0 353 897
Size: 353 544
PvuII CAG'CTG
Cuts at: 0 147 265 821 897
Size: 147 118 556 76
Fragments arranged by size:
556 147 118 76
SacI G_AGCT'C
Cuts at: 0 763 897
Size: 763 134
SfcI C'TryA_G
Cuts at: 0 266 349 897
Size: 266 83 548
Fragments arranged by size:
548 266 83
SmlI C'TyrA_G
Cuts at: 0 481 897
Size: 481 416
StuI AGG'CCT
Cuts at: 0 694 897
Size: 694 203
StyI C'CwwG_G
Cuts at: 0 399 831 897
Size: 399 432 66
Fragments arranged by size:
432 399 66
TatI w'GTAC_w
Cuts at: 0 378 897
Size: 378 519
Enzymes that do cut:
AcuI AflII AlfI BamHI BanII BbsI BmrI BsaBI
BsgI BsiHKAI Bsp1286I BspHI BspMI BsrDI BsrFI BstAPI
BstXI BstYI Bsu36I BtgZI BtsI EaeI EcoICRI Eco57MI
Hin4I HincII HindIII HpaI MscI MslI MspA1I PflMI
PsrI PstI PvuII SacI SfcI SmlI StuI StyI
TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AleI AloI AlwNI ApaI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BanI BbeI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiWI BsmI
BsmBI BspEI BsrBI BsrGI BssHII BssSI BstBI BstEII
BstZ17I BtgI ClaI CspCI DraI DraIII DrdI EagI
EarI EciI EcoNI EcoO109I EcoRI EcoRV FalI FseI
FspI FspAI HaeII KasI KpnI MfeI MluI MmeI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI NspI PacI PasI PciI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PvuI RsrII
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TaqII TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI