(Circular) (Six-base) MAPSORT of: pFN21AB8313.seq Check: 7030 from: 1 to: 5809 pFN21AB8313 5809 bp With 182 enzymes: SgfI * February 27, 2009 14:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2562 2562 Size: 5809 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3619 278 Size: 53 83 186 936 2083 2468 Fragments arranged by size: 2468 2083 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2859 3152 1937 Size: 922 293 4594 Fragments arranged by size: 4594 922 293 Acc65I G'GTAC_C Cuts at: 3130 3130 Size: 5809 AclI AA'CG_TT Cuts at: 1563 3936 4309 1563 Size: 2373 373 3063 Fragments arranged by size: 3063 2373 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2159 3872 5256 5524 1632 Size: 527 1713 1384 268 1917 Fragments arranged by size: 1917 1713 1384 527 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5612 AflIII A'CryG_T Cuts at: 1203 4708 5685 1203 Size: 3505 977 1327 Fragments arranged by size: 3505 1327 977 AgeI A'CCGG_T Cuts at: 4640 4640 Size: 5809 AhdI GACnn_n'nnGTC Cuts at: 4538 4538 Size: 5809 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5809 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2724 2781 1184 Size: 1540 57 4212 Fragments arranged by size: 4212 1540 57 AlwNI CAG_nnn'CTG Cuts at: 2203 2806 2900 2947 2990 3552 5124 2203 Size: 603 94 47 43 562 1572 2888 Fragments arranged by size: 2888 1572 603 562 94 47 43 ApaLI G'TGCA_C Cuts at: 3866 5022 3866 Size: 1156 4653 ApoI r'AATT_y Cuts at: 1493 3118 3344 3496 5605 1493 Size: 1625 226 152 2109 1697 Fragments arranged by size: 2109 1697 1625 226 152 AseI AT'TA_AT Cuts at: 160 1032 4363 160 Size: 872 3331 1606 Fragments arranged by size: 3331 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5809 AvaI C'yCGr_G Cuts at: 1870 1943 2162 2256 2476 3134 1870 Size: 73 219 94 220 658 4545 Fragments arranged by size: 4545 658 220 219 94 73 BamHI G'GATC_C Cuts at: 3139 3506 3139 Size: 367 5442 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3130 4585 618 Size: 325 217 627 1343 1455 1842 Fragments arranged by size: 1842 1455 1343 627 325 217 BanII G_rGCy'C Cuts at: 1391 2382 2538 3128 1391 Size: 991 156 590 4072 Fragments arranged by size: 4072 991 590 156 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4838 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4000 4034 1903 Size: 34 2063 34 3678 Fragments arranged by size: 3678 2063 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3701 4911 3701 Size: 1210 4599 BclI T'GATC_A Cuts at: 1548 1548 Size: 5809 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2067 4420 136 Size: 107 122 71 631 605 395 2353 1525 Fragments arranged by size: 2353 1525 631 605 395 122 107 71 BglII A'GATC_T Cuts at: 3042 5804 3042 Size: 2762 3047 BlpI GC'TnA_GC Cuts at: 3226 3226 Size: 5809 Bme1580I G_kGCm'C Cuts at: 1790 3870 5026 1790 Size: 2080 1156 2573 Fragments arranged by size: 2573 2080 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5809 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3540 4498 442 Size: 416 53 83 399 24 2123 958 1753 Fragments arranged by size: 2123 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5809 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2557 2557 Size: 5809 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2452 4469 1239 Size: 1213 2017 2579 Fragments arranged by size: 2579 2017 1213 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2369 3934 4799 5097 2369 Size: 1565 865 298 3081 Fragments arranged by size: 3081 1565 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3008 4472 882 Size: 617 1509 1464 2219 Fragments arranged by size: 2219 1509 1464 617 BsaAI yAC'GTr Cuts at: 493 1204 3600 493 Size: 711 2396 2702 Fragments arranged by size: 2702 2396 711 BsaBI GATnn'nnATC Cuts at: 5803 5803 Size: 5809 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3616 3998 4633 275 Size: 53 83 186 936 2083 382 635 1451 Fragments arranged by size: 2083 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2445 4242 4640 4914 5061 5472 2445 Size: 1797 398 274 147 411 2782 Fragments arranged by size: 2782 1797 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5584 Fragments arranged by size: 5584 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2372 2600 2678 2681 2684 1186 Size: 1186 228 78 3 3 4311 Fragments arranged by size: 4311 1186 228 78 3 3 BseYI C'CCAG_C Cuts at: 2338 2527 2872 3048 5012 5508 2338 Size: 189 345 176 1964 496 2639 Fragments arranged by size: 2639 1964 496 345 189 176 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2230 1163 Size: 573 494 4742 Fragments arranged by size: 4742 573 494 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2062 3259 4020 5048 1540 Size: 275 186 61 1197 761 1028 2301 Fragments arranged by size: 2301 1197 1028 761 275 186 61 BsiHKAI G_wGCw'C Cuts at: 2382 2468 2493 2538 3870 3955 5026 2382 Size: 86 25 45 1332 85 1071 3165 Fragments arranged by size: 3165 1332 1071 86 85 45 25 BsmI GAATG_Cn' Cuts at: 3320 3413 3320 Size: 93 5716 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2382 2468 2493 2538 3128 3870 3955 Size: 399 592 86 25 45 590 742 85 Cuts at: 3955 5026 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 592 590 399 86 85 45 25 BspEI T'CCGG_A Cuts at: 2445 5472 2445 Size: 3027 2782 BspHI T'CATG_A Cuts at: 3069 3698 5428 3069 Size: 629 1730 3450 Fragments arranged by size: 3450 1730 629 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2562 3164 844 Size: 1718 602 3489 Fragments arranged by size: 3489 1718 602 BsrBI CCG'CTC Cuts at: 1394 1823 3696 1394 Size: 429 1873 3507 Fragments arranged by size: 3507 1873 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4304 4478 65 Size: 1183 3056 174 1396 Fragments arranged by size: 3056 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4453 4640 1700 Size: 110 2643 187 2869 Fragments arranged by size: 2869 2643 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5809 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5809 BssSI C'ACGA_G Cuts at: 2345 3869 4881 2345 Size: 1524 1012 3273 Fragments arranged by size: 3273 1524 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5809 BstBI TT'CG_AA Cuts at: 4613 4613 Size: 5809 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5809 BstYI r'GATC_y Cuts at: 1972 2235 2319 3042 3139 3506 3891 3908 5360 Size: 263 84 723 97 367 385 17 1452 Cuts at: 5360 5446 5458 5804 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 723 385 367 346 263 97 86 84 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2062 2585 3009 513 Size: 552 939 58 523 424 3313 Fragments arranged by size: 3313 939 552 523 424 58 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5070 Fragments arranged by size: 5070 683 56 BtsI GCAGTG_nn' Cuts at: 2270 2642 3325 4119 4139 2270 Size: 372 683 794 20 3940 Fragments arranged by size: 3940 794 683 372 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5809 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5774 DraI TTT'AAA Cuts at: 3113 3113 Size: 5809 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4816 809 Size: 507 3500 1802 Fragments arranged by size: 3500 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2059 3256 4145 Size: 54 1425 50 275 18 229 1197 889 Cuts at: 4145 5774 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1197 889 275 229 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2059 3256 1537 Size: 275 247 1197 4090 Fragments arranged by size: 4090 1197 275 247 EarI CTCTTCn'nnn_ Cuts at: 1353 2543 3739 1353 Size: 1190 1196 3423 Fragments arranged by size: 3423 1196 1190 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2696 4396 4770 4916 387 Size: 1064 138 1107 1700 374 146 1280 Fragments arranged by size: 1700 1280 1107 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2380 2536 2380 Size: 156 5653 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2159 2452 3872 4469 5256 5524 1239 Size: 393 527 293 1420 597 787 268 1524 Fragments arranged by size: 1524 1420 787 597 527 393 293 268 EcoO109I rG'GnC_Cy Cuts at: 2143 2302 2897 2143 Size: 159 595 5055 Fragments arranged by size: 5055 595 159 EcoRI G'AATT_C Cuts at: 3118 3496 3118 Size: 378 5431 FspI TGC'GCA Cuts at: 4315 4315 Size: 5809 HaeII r_GCGC'y Cuts at: 1678 2893 4956 1678 Size: 1215 2063 2531 Fragments arranged by size: 2531 2063 1215 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2063 2095 4450 4482 4524 Size: 32 135 32 491 32 2355 32 42 Cuts at: 4524 4556 1373 Size: 32 2626 Fragments arranged by size: 2626 2355 491 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3153 3399 669 Size: 988 281 1215 246 3079 Fragments arranged by size: 3079 1215 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5809 HpaI GTT'AAC Cuts at: 3399 3399 Size: 5809 KpnI G_GTAC'C Cuts at: 3134 3134 Size: 5809 MfeI C'AATT_G Cuts at: 3408 3408 Size: 5809 MluI A'CGCG_T Cuts at: 5685 5685 Size: 5809 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2847 4923 5107 1305 Size: 1542 2076 184 2007 Fragments arranged by size: 2076 2007 1542 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3987 Fragments arranged by size: 3987 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3767 4126 4285 518 Size: 648 2601 359 159 2042 Fragments arranged by size: 2601 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2698 3225 3902 5050 5295 1606 Size: 1092 527 677 1148 245 2120 Fragments arranged by size: 2120 1148 1092 677 527 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4318 Fragments arranged by size: 4318 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5809 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5809 NotI GC'GGCC_GC Cuts at: 3256 3256 Size: 5809 NspI r_CATG'y Cuts at: 1132 1229 2602 3167 4712 1132 Size: 97 1373 565 1545 2229 Fragments arranged by size: 2229 1545 1373 565 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5809 PciI A'CATG_T Cuts at: 4708 4708 Size: 5809 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5809 PmeI GTTT'AAAC Cuts at: 3113 3113 Size: 5809 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3878 3910 5391 5423 3878 Size: 32 1481 32 4264 Fragments arranged by size: 4264 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2143 2302 2143 Size: 159 5650 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5809 PsiI TTA'TAA Cuts at: 3379 3379 Size: 5809 PspXI vC'TCGA_Gb Cuts at: 2162 2476 2162 Size: 314 5495 PstI C_TGCA'G Cuts at: 830 2277 2559 2703 3161 830 Size: 1447 282 144 458 3478 Fragments arranged by size: 3478 1447 458 282 144 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5809 PvuII CAG'CTG Cuts at: 2698 2698 Size: 5809 SacI G_AGCT'C Cuts at: 2382 2538 2382 Size: 156 5653 SalI G'TCGA_C Cuts at: 1936 3151 1936 Size: 1215 4594 SanDI GG'GwC_CC Cuts at: 2302 2302 Size: 5809 SapI GCTCTTCn'nnn_ Cuts at: 2543 2543 Size: 5809 SbfI CC_TGCA'GG Cuts at: 3161 3161 Size: 5809 ScaI AGT'ACT Cuts at: 2741 4057 5769 2741 Size: 1316 1712 2781 Fragments arranged by size: 2781 1712 1316 SfcI C'TryA_G Cuts at: 826 1045 2273 2555 2699 2956 3157 4292 4973 Size: 219 1228 282 144 257 201 1135 681 Cuts at: 4973 5164 826 Size: 191 1471 Fragments arranged by size: 1471 1228 1135 681 282 257 219 201 191 144 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5809 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5809 SmaI CCC'GGG Cuts at: 1872 2258 3136 1872 Size: 386 878 4545 Fragments arranged by size: 4545 878 386 SmlI C'TyrA_G Cuts at: 820 1017 1943 2162 2384 2476 3913 4814 5076 Size: 197 926 219 222 92 1437 901 262 Cuts at: 5076 820 Size: 1553 Fragments arranged by size: 1553 1437 926 901 262 222 219 197 92 SnaBI TAC'GTA Cuts at: 493 3600 493 Size: 3107 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5809 SphI G_CATG'C Cuts at: 1132 3167 1132 Size: 2035 3774 SspI AAT'ATT Cuts at: 5 52 3733 5 Size: 47 3681 2081 Fragments arranged by size: 3681 2081 47 StuI AGG'CCT Cuts at: 1517 2522 1517 Size: 1005 4804 StyI C'CwwG_G Cuts at: 513 1065 2004 3248 513 Size: 552 939 1244 3074 Fragments arranged by size: 3074 1244 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2451 3836 3853 4006 4191 4636 325 Size: 1255 871 1385 17 153 185 445 1498 Fragments arranged by size: 1498 1385 1255 871 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2739 4055 5767 Size: 274 80 33 51 1443 762 1316 1712 Cuts at: 5767 96 Size: 138 Fragments arranged by size: 1712 1443 1316 762 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2192 2224 5769 5801 2192 Size: 32 3545 32 2200 Fragments arranged by size: 3545 2200 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5809 XbaI T'CTAG_A Cuts at: 3145 3145 Size: 5809 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2116 2116 Size: 5809 XhoI C'TCGA_G Cuts at: 1943 2162 2476 1943 Size: 219 314 5276 Fragments arranged by size: 5276 314 219 XmaI C'CCGG_G Cuts at: 1870 2256 3134 1870 Size: 386 878 4545 Fragments arranged by size: 4545 878 386 XmnI GAAnn'nnTTC Cuts at: 3938 3938 Size: 5809 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3617 276 Size: 53 83 186 936 2083 2468 Fragments arranged by size: 2468 2083 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI