(Circular) (Six-base) MAPSORT of: pFN21AB8304.seq Check: 8003 from: 1 to: 5599 pFN21AB8304 5599 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3409 278 Size: 53 83 186 936 1873 2468 Fragments arranged by size: 2468 1873 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2942 1937 Size: 1005 4594 Acc65I G'GTAC_C Cuts at: 2505 2920 2505 Size: 415 5184 AclI AA'CG_TT Cuts at: 1563 3726 4099 1563 Size: 2163 373 3063 Fragments arranged by size: 3063 2163 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3662 5046 5314 1632 Size: 2030 1384 268 1917 Fragments arranged by size: 2030 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2099 2168 820 Size: 197 1082 69 4251 Fragments arranged by size: 4251 1082 197 69 AflIII A'CryG_T Cuts at: 1203 4498 5475 1203 Size: 3295 977 1327 Fragments arranged by size: 3295 1327 977 AgeI A'CCGG_T Cuts at: 4430 4430 Size: 5599 AhdI GACnn_n'nnGTC Cuts at: 4328 4328 Size: 5599 AleI CACnn'nnGTG Cuts at: 1166 2552 1166 Size: 1386 4213 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5599 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2454 2486 2454 Size: 32 5567 AlwNI CAG_nnn'CTG Cuts at: 2401 3342 4914 2401 Size: 941 1572 3086 Fragments arranged by size: 3086 1572 941 ApaI G_GGCC'C Cuts at: 2608 2737 2608 Size: 129 5470 ApaLI G'TGCA_C Cuts at: 3656 4812 3656 Size: 1156 4443 ApoI r'AATT_y Cuts at: 1493 2246 2270 2908 3134 3286 5395 1493 Size: 753 24 638 226 152 2109 1697 Fragments arranged by size: 2109 1697 753 638 226 152 24 AseI AT'TA_AT Cuts at: 160 1032 4153 160 Size: 872 3121 1606 Fragments arranged by size: 3121 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5599 AvaI C'yCGr_G Cuts at: 1870 1943 2924 1870 Size: 73 981 4545 Fragments arranged by size: 4545 981 73 BamHI G'GATC_C Cuts at: 2929 3296 2929 Size: 367 5232 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2505 2920 4375 618 Size: 325 217 627 718 415 1455 1842 Fragments arranged by size: 1842 1455 718 627 415 325 217 BanII G_rGCy'C Cuts at: 1391 2515 2608 2737 2918 1391 Size: 1124 93 129 181 4072 Fragments arranged by size: 4072 1124 181 129 93 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4628 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3790 3824 1903 Size: 34 1853 34 3678 Fragments arranged by size: 3678 1853 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3491 4701 3491 Size: 1210 4389 BclI T'GATC_A Cuts at: 1548 1548 Size: 5599 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4210 136 Size: 107 122 71 631 605 2538 1525 Fragments arranged by size: 2538 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2157 2260 5594 2157 Size: 103 3334 2162 Fragments arranged by size: 3334 2162 103 BlpI GC'TnA_GC Cuts at: 3016 3016 Size: 5599 Bme1580I G_kGCm'C Cuts at: 1790 2045 2139 2608 2737 3660 4816 1790 Size: 255 94 469 129 923 1156 2573 Fragments arranged by size: 2573 1156 923 469 255 129 94 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5599 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2133 3330 4288 Size: 416 53 83 399 24 716 1197 958 Cuts at: 4288 442 Size: 1753 Fragments arranged by size: 1753 1197 958 716 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2435 1056 Size: 1379 4220 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2324 2584 2637 4259 1239 Size: 1085 260 53 1622 2579 Fragments arranged by size: 2579 1622 1085 260 53 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2249 3724 4589 4887 2249 Size: 1475 865 298 2961 Fragments arranged by size: 2961 1475 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2376 4262 882 Size: 617 877 1886 2219 Fragments arranged by size: 2219 1886 877 617 BsaAI yAC'GTr Cuts at: 493 1204 2046 3390 493 Size: 711 842 1344 2702 Fragments arranged by size: 2702 1344 842 711 BsaBI GATnn'nnATC Cuts at: 5593 5593 Size: 5599 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3406 3788 4423 275 Size: 53 83 186 936 1873 382 635 1451 Fragments arranged by size: 1873 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2643 4032 4430 4704 4851 5262 2643 Size: 1389 398 274 147 411 2980 Fragments arranged by size: 2980 1389 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2076 2106 2454 2484 1178 Size: 30 165 30 673 30 348 30 4293 Fragments arranged by size: 4293 673 348 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5599 BseYI C'CCAG_C Cuts at: 4802 5298 4802 Size: 496 5103 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2237 2789 1163 Size: 573 501 552 3973 Fragments arranged by size: 3973 573 552 501 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3049 3810 4838 1540 Size: 275 186 1048 761 1028 2301 Fragments arranged by size: 2301 1048 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2515 3660 3745 4816 2515 Size: 1145 85 1071 3298 Fragments arranged by size: 3298 1145 1071 85 BsiWI C'GTAC_G Cuts at: 2046 2046 Size: 5599 BsmI GAATG_Cn' Cuts at: 2298 3110 3203 2298 Size: 812 93 4694 Fragments arranged by size: 4694 812 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2045 2139 2515 2608 2737 2918 3660 Size: 399 255 94 376 93 129 181 742 Cuts at: 3660 3745 4816 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 376 255 181 129 94 93 85 BspEI T'CCGG_A Cuts at: 2643 5262 2643 Size: 2619 2980 BspHI T'CATG_A Cuts at: 3488 5218 3488 Size: 1730 3869 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2954 844 Size: 2110 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3486 1394 Size: 429 1663 3507 Fragments arranged by size: 3507 1663 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4094 4268 65 Size: 1183 2846 174 1396 Fragments arranged by size: 2846 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4243 4430 1700 Size: 110 2433 187 2869 Fragments arranged by size: 2869 2433 187 110 BsrGI T'GTAC_A Cuts at: 96 2058 96 Size: 1962 3637 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5599 BssSI C'ACGA_G Cuts at: 3659 4671 3659 Size: 1012 4587 BstAPI GCAn_nnn'nTGC Cuts at: 794 2803 794 Size: 2009 3590 BstBI TT'CG_AA Cuts at: 4403 4403 Size: 5599 BstEII G'GTnAC_C Cuts at: 1184 2494 1184 Size: 1310 4289 BstXI CCAn_nnnn'nTGG Cuts at: 2554 2554 Size: 5599 BstYI r'GATC_y Cuts at: 1972 2157 2260 2646 2929 3296 3681 3698 5150 Size: 185 103 386 283 367 385 17 1452 Cuts at: 5150 5236 5248 5594 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 386 385 367 346 283 185 103 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4108 Fragments arranged by size: 4108 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4860 Fragments arranged by size: 4860 683 56 BtsI GCAGTG_nn' Cuts at: 3115 3909 3929 3115 Size: 794 20 4785 Fragments arranged by size: 4785 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5599 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5564 DraI TTT'AAA Cuts at: 2903 2903 Size: 5599 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4606 809 Size: 507 3290 1802 Fragments arranged by size: 3290 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2684 2832 3046 Size: 54 1425 50 275 18 854 148 214 Cuts at: 3046 3935 5564 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 854 275 214 148 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3046 1537 Size: 275 1234 4090 Fragments arranged by size: 4090 1234 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2310 2645 3529 1353 Size: 957 335 884 3423 Fragments arranged by size: 3423 957 884 335 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2099 4186 4560 4706 387 Size: 1064 138 510 2087 374 146 1280 Fragments arranged by size: 2087 1280 1064 510 374 146 138 EcoICRI GAG'CTC Cuts at: 2513 2513 Size: 5599 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2324 2584 2637 3662 4259 5046 5314 Size: 393 692 260 53 1025 597 787 268 Cuts at: 5314 1239 Size: 1524 Fragments arranged by size: 1524 1025 787 692 597 393 268 260 53 EcoO109I rG'GnC_Cy Cuts at: 2344 2371 2605 2664 2344 Size: 27 234 59 5279 Fragments arranged by size: 5279 234 59 27 EcoRI G'AATT_C Cuts at: 2908 3286 2908 Size: 378 5221 FspI TGC'GCA Cuts at: 4105 4105 Size: 5599 HaeII r_GCGC'y Cuts at: 1678 2099 4746 1678 Size: 421 2647 2531 Fragments arranged by size: 2647 2531 421 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2581 2613 4240 4272 4314 Size: 32 135 32 1009 32 1627 32 42 Cuts at: 4314 4346 1373 Size: 32 2626 Fragments arranged by size: 2626 1627 1009 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2079 2943 3189 669 Size: 988 281 141 864 246 3079 Fragments arranged by size: 3079 988 864 281 246 141 HindIII A'AGCT_T Cuts at: 748 748 Size: 5599 HpaI GTT'AAC Cuts at: 3189 3189 Size: 5599 KpnI G_GTAC'C Cuts at: 2509 2924 2509 Size: 415 5184 MfeI C'AATT_G Cuts at: 3198 3198 Size: 5599 MluI A'CGCG_T Cuts at: 5475 5475 Size: 5599 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4713 4897 1305 Size: 3408 184 2007 Fragments arranged by size: 3408 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2686 10 Size: 54 1425 343 854 2923 Fragments arranged by size: 2923 1425 854 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2552 3557 3916 4075 518 Size: 648 1386 1005 359 159 2042 Fragments arranged by size: 2042 1386 1005 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2335 2552 3015 3692 4840 5085 1606 Size: 729 217 463 677 1148 245 2120 Fragments arranged by size: 2120 1148 729 677 463 245 217 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4108 Fragments arranged by size: 4108 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5599 NheI G'CTAG_C Cuts at: 1052 2431 1052 Size: 1379 4220 NotI GC'GGCC_GC Cuts at: 3046 3046 Size: 5599 NspI r_CATG'y Cuts at: 1132 1229 2957 4502 1132 Size: 97 1728 1545 2229 Fragments arranged by size: 2229 1728 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5599 PciI A'CATG_T Cuts at: 4498 4498 Size: 5599 PflMI CCAn_nnn'nTGG Cuts at: 1108 2023 2491 1108 Size: 915 468 4216 Fragments arranged by size: 4216 915 468 PfoI T'CCnGG_A Cuts at: 2862 2862 Size: 5599 PmeI GTTT'AAAC Cuts at: 2903 2903 Size: 5599 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2454 2486 3668 3700 5181 5213 2454 Size: 32 1182 32 1481 32 2840 Fragments arranged by size: 2840 1481 1182 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2371 2664 2371 Size: 293 5306 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5599 PsiI TTA'TAA Cuts at: 3169 3169 Size: 5599 PspOMI G'GGCC_C Cuts at: 2604 2733 2604 Size: 129 5470 PstI C_TGCA'G Cuts at: 830 2951 830 Size: 2121 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5599 PvuII CAG'CTG Cuts at: 2335 2552 2335 Size: 217 5382 SacI G_AGCT'C Cuts at: 2515 2515 Size: 5599 SalI G'TCGA_C Cuts at: 1936 2941 1936 Size: 1005 4594 SanDI GG'GwC_CC Cuts at: 2664 2664 Size: 5599 SapI GCTCTTCn'nnn_ Cuts at: 2645 2645 Size: 5599 SbfI CC_TGCA'GG Cuts at: 2951 2951 Size: 5599 ScaI AGT'ACT Cuts at: 2143 3847 5559 2143 Size: 1704 1712 2183 Fragments arranged by size: 2183 1712 1704 SexAI A'CCwGG_T Cuts at: 2235 2235 Size: 5599 SfcI C'TryA_G Cuts at: 826 1045 2947 4082 4763 4954 826 Size: 219 1902 1135 681 191 1471 Fragments arranged by size: 1902 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5599 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5599 SmaI CCC'GGG Cuts at: 1872 2926 1872 Size: 1054 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2099 2168 2264 3703 4604 4866 Size: 197 926 156 69 96 1439 901 262 Cuts at: 4866 820 Size: 1553 Fragments arranged by size: 1553 1439 926 901 262 197 156 96 69 SnaBI TAC'GTA Cuts at: 493 3390 493 Size: 2897 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5599 SphI G_CATG'C Cuts at: 1132 2957 1132 Size: 1825 3774 SspI AAT'ATT Cuts at: 5 52 2191 2540 3523 5 Size: 47 2139 349 983 2081 Fragments arranged by size: 2139 2081 983 349 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5599 StyI C'CwwG_G Cuts at: 513 1065 2004 2835 3038 513 Size: 552 939 831 203 3074 Fragments arranged by size: 3074 939 831 552 203 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2498 3626 3643 3796 3981 4426 325 Size: 1255 918 1128 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1128 918 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2058 2141 3845 Size: 274 80 33 51 1443 81 83 1704 Cuts at: 3845 5557 96 Size: 1712 138 Fragments arranged by size: 1712 1704 1443 274 138 83 81 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2076 2108 2284 2316 5559 5591 2076 Size: 32 176 32 3243 32 2084 Fragments arranged by size: 3243 2084 176 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5599 XbaI T'CTAG_A Cuts at: 2935 2935 Size: 5599 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2842 2842 Size: 5599 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5599 XmaI C'CCGG_G Cuts at: 1870 2924 1870 Size: 1054 4545 XmnI GAAnn'nnTTC Cuts at: 3728 3728 Size: 5599 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3407 276 Size: 53 83 186 936 1873 2468 Fragments arranged by size: 2468 1873 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsmBI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SfiI SfoI SrfI SwaI