(Circular) (Six-base) MAPSORT of: pFN21AB8303.seq Check: 5122 from: 1 to: 6505 pFN21AB8303 6505 bp With 182 enzymes: SgfI * February 27, 2009 14:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3121 3142 3548 3561 3121 Size: 21 406 13 6065 Fragments arranged by size: 6065 406 21 13 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4315 278 Size: 53 83 186 936 2779 2468 Fragments arranged by size: 2779 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2340 2526 3594 3848 1937 Size: 403 186 1068 254 4594 Fragments arranged by size: 4594 1068 403 254 186 Acc65I G'GTAC_C Cuts at: 3826 3826 Size: 6505 AclI AA'CG_TT Cuts at: 1563 4632 5005 1563 Size: 3069 373 3063 Fragments arranged by size: 3069 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2227 2690 2807 3293 3407 3638 3797 4568 Size: 595 463 117 486 114 231 159 771 Cuts at: 4568 5952 6220 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 771 595 486 463 268 231 159 117 114 AfeI AGC'GCT Cuts at: 2648 2648 Size: 6505 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6308 AflIII A'CryG_T Cuts at: 1203 2898 3162 5404 6381 1203 Size: 1695 264 2242 977 1327 Fragments arranged by size: 2242 1695 1327 977 264 AgeI A'CCGG_T Cuts at: 5336 5336 Size: 6505 AhdI GACnn_n'nnGTC Cuts at: 5234 5234 Size: 6505 AleI CACnn'nnGTG Cuts at: 1166 3713 1166 Size: 2547 3958 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3731 1184 Size: 2547 3958 AlwNI CAG_nnn'CTG Cuts at: 2519 3004 3136 3409 4248 5820 2519 Size: 485 132 273 839 1572 3204 Fragments arranged by size: 3204 1572 839 485 273 132 ApaLI G'TGCA_C Cuts at: 3548 4562 5718 3548 Size: 1014 1156 4335 Fragments arranged by size: 4335 1156 1014 ApoI r'AATT_y Cuts at: 1493 3814 4040 4192 6301 1493 Size: 2321 226 152 2109 1697 Fragments arranged by size: 2321 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5059 160 Size: 872 4027 1606 Fragments arranged by size: 4027 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6505 AvaI C'yCGr_G Cuts at: 1870 1943 3113 3830 1870 Size: 73 1170 717 4545 Fragments arranged by size: 4545 1170 717 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2705 2738 2705 Size: 33 6472 BamHI G'GATC_C Cuts at: 3835 4202 3835 Size: 367 6138 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2791 3074 3446 3707 3826 Size: 325 217 627 1004 283 372 261 119 Cuts at: 3826 5281 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 1004 627 372 325 283 261 217 119 BanII G_rGCy'C Cuts at: 1391 2513 2748 3297 3824 1391 Size: 1122 235 549 527 4072 Fragments arranged by size: 4072 1122 549 527 235 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5534 BbvCI CC'TCA_GC Cuts at: 3095 3095 Size: 6505 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4696 4730 1903 Size: 34 50 34 2675 34 3678 Fragments arranged by size: 3678 2675 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4397 5607 4397 Size: 1210 5295 BclI T'GATC_A Cuts at: 1548 2196 2379 3453 1548 Size: 648 183 1074 4600 Fragments arranged by size: 4600 1074 648 183 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5116 136 Size: 107 122 71 631 605 3444 1525 Fragments arranged by size: 3444 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3009 6500 3009 Size: 3491 3014 BlpI GC'TnA_GC Cuts at: 3519 3922 3519 Size: 403 6102 Bme1580I G_kGCm'C Cuts at: 1790 2233 2796 3077 3552 4566 5722 1790 Size: 443 563 281 475 1014 1156 2573 Fragments arranged by size: 2573 1156 1014 563 475 443 281 BmgBI CAC'GTC Cuts at: 1318 2047 1318 Size: 729 5776 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4236 5194 442 Size: 416 53 83 399 24 2819 958 1753 Fragments arranged by size: 2819 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6505 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2336 2468 2780 3158 3662 5165 1239 Size: 1097 132 312 378 504 1503 2579 Fragments arranged by size: 2579 1503 1097 504 378 312 132 Bpu10I CC'TnA_GC Cuts at: 3095 3095 Size: 6505 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3590 4630 5495 5793 3590 Size: 1040 865 298 4302 Fragments arranged by size: 4302 1040 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2074 2678 2756 3730 5168 882 Size: 617 575 604 78 974 1438 2219 Fragments arranged by size: 2219 1438 974 617 604 575 78 BsaAI yAC'GTr Cuts at: 493 1204 2476 2619 4296 493 Size: 711 1272 143 1677 2702 Fragments arranged by size: 2702 1677 1272 711 143 BsaBI GATnn'nnATC Cuts at: 6499 6499 Size: 6505 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3186 4312 4694 5329 Size: 53 83 186 936 1653 1126 382 635 Cuts at: 5329 275 Size: 1451 Fragments arranged by size: 1653 1451 1126 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3015 4938 5336 5610 5757 6168 3015 Size: 1923 398 274 147 411 3352 Fragments arranged by size: 3352 1923 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3149 3179 1178 Size: 30 165 30 1746 30 4504 Fragments arranged by size: 4504 1746 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3665 3761 1186 Size: 2479 96 3930 Fragments arranged by size: 3930 2479 96 BseYI C'CCAG_C Cuts at: 2479 2728 2993 3353 3376 3508 3751 3800 5708 Size: 249 265 360 23 132 243 49 1908 Cuts at: 5708 6204 2479 Size: 496 2780 Fragments arranged by size: 2780 1908 496 360 265 249 243 132 49 23 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2909 2972 3089 3425 3653 1163 Size: 573 1173 63 117 336 228 4015 Fragments arranged by size: 4015 1173 573 336 228 117 63 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2131 2170 2932 3955 4716 5744 Size: 275 186 130 39 762 1023 761 1028 Cuts at: 5744 1540 Size: 2301 Fragments arranged by size: 2301 1028 1023 762 761 275 186 130 39 BsiHKAI G_wGCw'C Cuts at: 2748 3297 3525 3552 4566 4651 5722 2748 Size: 549 228 27 1014 85 1071 3531 Fragments arranged by size: 3531 1071 1014 549 228 85 27 BsiWI C'GTAC_G Cuts at: 2619 2619 Size: 6505 BsmI GAATG_Cn' Cuts at: 2112 2988 4016 4109 2112 Size: 876 1028 93 4508 Fragments arranged by size: 4508 1028 876 93 BsmBI CGTCTCn'nnnn_ Cuts at: 3323 3572 3323 Size: 249 6256 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2233 2513 2748 2796 3077 3297 3525 Size: 399 443 280 235 48 281 220 228 Cuts at: 3525 3552 3824 4566 4651 5722 1391 Size: 27 272 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 443 399 281 280 272 235 228 220 85 48 27 BspEI T'CCGG_A Cuts at: 3015 6168 3015 Size: 3153 3352 BspHI T'CATG_A Cuts at: 3456 4394 6124 3456 Size: 938 1730 3837 Fragments arranged by size: 3837 1730 938 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2607 3121 3142 3548 3561 3860 844 Size: 1763 514 21 406 13 299 3489 Fragments arranged by size: 3489 1763 514 406 299 21 13 BsrBI CCG'CTC Cuts at: 1394 1823 4392 1394 Size: 429 2569 3507 Fragments arranged by size: 3507 2569 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2073 3584 5000 5174 65 Size: 1183 825 1511 1416 174 1396 Fragments arranged by size: 1511 1416 1396 1183 825 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2036 3316 5149 5336 1700 Size: 110 226 1280 1833 187 2869 Fragments arranged by size: 2869 1833 1280 226 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6505 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6505 BssSI C'ACGA_G Cuts at: 2681 4565 5577 2681 Size: 1884 1012 3609 Fragments arranged by size: 3609 1884 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3004 3409 3529 794 Size: 2210 405 120 3770 Fragments arranged by size: 3770 2210 405 120 BstBI TT'CG_AA Cuts at: 5309 5309 Size: 6505 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6505 BstXI CCAn_nnnn'nTGG Cuts at: 2314 3262 2314 Size: 948 5557 BstYI r'GATC_y Cuts at: 1972 2871 3009 3090 3835 4202 4587 4604 6056 Size: 899 138 81 745 367 385 17 1452 Cuts at: 6056 6142 6154 6500 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 899 745 385 367 346 138 86 81 17 12 Bsu36I CC'TnA_GG Cuts at: 3417 3417 Size: 6505 BtgI C'CryG_G Cuts at: 513 1065 2688 2808 513 Size: 552 1623 120 4210 Fragments arranged by size: 4210 1623 552 120 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5766 Fragments arranged by size: 5766 683 56 BtsI GCAGTG_nn' Cuts at: 2221 4021 4815 4835 2221 Size: 1800 794 20 3891 Fragments arranged by size: 3891 1800 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6505 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6470 DraI TTT'AAA Cuts at: 3809 3809 Size: 6505 DraIII CAC_nnn'GTG Cuts at: 3631 3631 Size: 6505 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3664 5512 809 Size: 507 2348 1848 1802 Fragments arranged by size: 2348 1848 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2038 2167 3082 Size: 54 1425 50 275 18 208 129 915 Cuts at: 3082 3318 3952 4841 6470 8 Size: 236 634 889 1629 43 Fragments arranged by size: 1629 1425 915 889 634 275 236 208 129 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2167 3952 1537 Size: 275 355 1785 4090 Fragments arranged by size: 4090 1785 355 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4435 1353 Size: 3082 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5092 5466 5612 387 Size: 1064 138 3503 374 146 1280 Fragments arranged by size: 3503 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2746 3295 2746 Size: 549 5956 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2227 2336 2468 2690 2780 2807 3158 Size: 393 595 109 132 222 90 27 351 Cuts at: 3158 3293 3407 3638 3662 3797 4568 5165 5952 Size: 135 114 231 24 135 771 597 787 Cuts at: 5952 6220 1239 Size: 268 1524 Fragments arranged by size: 1524 787 771 597 595 393 351 268 231 222 135 135 132 114 109 90 27 24 EcoNI CCTnn'n_nnAGG Cuts at: 3677 3791 3677 Size: 114 6391 EcoO109I rG'GnC_Cy Cuts at: 3700 3784 3796 3700 Size: 84 12 6409 Fragments arranged by size: 6409 84 12 EcoRI G'AATT_C Cuts at: 3814 4192 3814 Size: 378 6127 FseI GG_CCGG'CC Cuts at: 2040 2040 Size: 6505 FspI TGC'GCA Cuts at: 5011 5011 Size: 6505 HaeII r_GCGC'y Cuts at: 1678 2650 5652 1678 Size: 972 3002 2531 Fragments arranged by size: 3002 2531 972 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3149 3181 5146 5178 5220 Size: 32 135 32 1577 32 1965 32 42 Cuts at: 5220 5252 1373 Size: 32 2626 Fragments arranged by size: 2626 1965 1577 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3304 3849 4095 669 Size: 988 281 1366 545 246 3079 Fragments arranged by size: 3079 1366 988 545 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6505 HpaI GTT'AAC Cuts at: 4095 4095 Size: 6505 KpnI G_GTAC'C Cuts at: 3830 3830 Size: 6505 MfeI C'AATT_G Cuts at: 4104 4104 Size: 6505 MluI A'CGCG_T Cuts at: 6381 6381 Size: 6505 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5619 5803 1305 Size: 4314 184 2007 Fragments arranged by size: 4314 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4683 Fragments arranged by size: 4683 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2276 3555 3713 4463 4822 4981 518 Size: 648 1110 1279 158 750 359 159 2042 Fragments arranged by size: 2042 1279 1110 750 648 359 159 158 MspA1I CmG'CkG Cuts at: 1606 3099 3106 3205 3529 3921 4598 5746 5991 Size: 1493 7 99 324 392 677 1148 245 Cuts at: 5991 1606 Size: 2120 Fragments arranged by size: 2120 1493 1148 677 392 324 245 99 7 NaeI GCC'GGC Cuts at: 2038 3318 2038 Size: 1280 5225 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5953 NdeI CA'TA_TG Cuts at: 387 387 Size: 6505 NgoMIV G'CCGG_C Cuts at: 2036 3316 2036 Size: 1280 5225 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6505 NotI GC'GGCC_GC Cuts at: 3952 3952 Size: 6505 NspI r_CATG'y Cuts at: 1132 1229 2902 3166 3316 3863 5408 1132 Size: 97 1673 264 150 547 1545 2229 Fragments arranged by size: 2229 1673 1545 547 264 150 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6505 PciI A'CATG_T Cuts at: 2898 3162 5404 2898 Size: 264 2242 3999 Fragments arranged by size: 3999 2242 264 PflMI CCAn_nnn'nTGG Cuts at: 1108 2047 2479 1108 Size: 939 432 5134 Fragments arranged by size: 5134 939 432 PfoI T'CCnGG_A Cuts at: 2184 2757 3642 3728 2184 Size: 573 885 86 4961 Fragments arranged by size: 4961 885 573 86 PmeI GTTT'AAAC Cuts at: 3809 3809 Size: 6505 PmlI CAC'GTG Cuts at: 2476 2476 Size: 6505 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4574 4606 6087 6119 4574 Size: 32 1481 32 4960 Fragments arranged by size: 4960 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3700 3700 Size: 6505 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6505 PsiI TTA'TAA Cuts at: 2541 4075 2541 Size: 1534 4971 PstI C_TGCA'G Cuts at: 830 3009 3857 830 Size: 2179 848 3478 Fragments arranged by size: 3478 2179 848 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6505 PvuII CAG'CTG Cuts at: 3205 3529 3205 Size: 324 6181 SacI G_AGCT'C Cuts at: 2748 3297 2748 Size: 549 5956 SalI G'TCGA_C Cuts at: 1936 3847 1936 Size: 1911 4594 SbfI CC_TGCA'GG Cuts at: 3857 3857 Size: 6505 ScaI AGT'ACT Cuts at: 4753 6465 4753 Size: 1712 4793 SfcI C'TryA_G Cuts at: 826 1045 3005 3595 3853 4988 5669 5860 826 Size: 219 1960 590 258 1135 681 191 1471 Fragments arranged by size: 1960 1471 1135 681 590 258 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6505 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6505 SmaI CCC'GGG Cuts at: 1872 3832 1872 Size: 1960 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3113 3605 4609 5510 5772 820 Size: 197 926 1170 492 1004 901 262 1553 Fragments arranged by size: 1553 1170 1004 926 901 492 262 197 SnaBI TAC'GTA Cuts at: 493 4296 493 Size: 3803 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6505 SphI G_CATG'C Cuts at: 1132 3863 1132 Size: 2731 3774 SspI AAT'ATT Cuts at: 5 52 4429 5 Size: 47 4377 2081 Fragments arranged by size: 4377 2081 47 StuI AGG'CCT Cuts at: 1517 2459 2924 1517 Size: 942 465 5098 Fragments arranged by size: 5098 942 465 StyI C'CwwG_G Cuts at: 513 1065 2028 3189 3711 3944 513 Size: 552 963 1161 522 233 3074 Fragments arranged by size: 3074 1161 963 552 522 233 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2145 2174 2306 2571 2834 2971 4532 Size: 1255 565 29 132 265 263 137 1561 Cuts at: 4532 4549 4702 4887 5332 325 Size: 17 153 185 445 1498 Fragments arranged by size: 1561 1498 1255 565 445 265 263 185 153 137 132 29 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3159 4751 6463 Size: 274 80 33 51 1443 1182 1592 1712 Cuts at: 6463 96 Size: 138 Fragments arranged by size: 1712 1592 1443 1182 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6465 6497 6465 Size: 32 6473 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6505 XbaI T'CTAG_A Cuts at: 3841 3841 Size: 6505 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3718 3718 Size: 6505 XhoI C'TCGA_G Cuts at: 1943 3113 1943 Size: 1170 5335 XmaI C'CCGG_G Cuts at: 1870 3830 1870 Size: 1960 4545 XmnI GAAnn'nnTTC Cuts at: 4634 4634 Size: 6505 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4313 276 Size: 53 83 186 936 2779 2468 Fragments arranged by size: 2779 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BbeI BfrBI BplI BstZ17I EcoRV FalI FspAI KasI NarI NruI NsiI PacI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI