(Circular) (Six-base) MAPSORT of: pFN21AB8302.seq Check: 6194 from: 1 to: 5380 pFN21AB8302 5380 bp With 182 enzymes: SgfI * February 27, 2009 14:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2013 2013 Size: 5380 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3190 278 Size: 53 83 186 936 1654 2468 Fragments arranged by size: 2468 1654 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2723 1937 Size: 786 4594 Acc65I G'GTAC_C Cuts at: 2701 2701 Size: 5380 AclI AA'CG_TT Cuts at: 1563 3507 3880 1563 Size: 1944 373 3063 Fragments arranged by size: 3063 1944 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3443 4827 5095 1632 Size: 1811 1384 268 1917 Fragments arranged by size: 1917 1811 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5183 AflIII A'CryG_T Cuts at: 1203 2514 2551 4279 5256 1203 Size: 1311 37 1728 977 1327 Fragments arranged by size: 1728 1327 1311 977 37 AgeI A'CCGG_T Cuts at: 4211 4211 Size: 5380 AhdI GACnn_n'nnGTC Cuts at: 4109 4109 Size: 5380 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5380 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2204 1184 Size: 1020 4360 AlwNI CAG_nnn'CTG Cuts at: 2197 3123 4695 2197 Size: 926 1572 2882 Fragments arranged by size: 2882 1572 926 ApaLI G'TGCA_C Cuts at: 3437 4593 3437 Size: 1156 4224 ApoI r'AATT_y Cuts at: 1493 2108 2171 2612 2689 2915 3067 5176 1493 Size: 615 63 441 77 226 152 2109 1697 Fragments arranged by size: 2109 1697 615 441 226 152 77 63 AseI AT'TA_AT Cuts at: 160 1032 3934 160 Size: 872 2902 1606 Fragments arranged by size: 2902 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5380 AvaI C'yCGr_G Cuts at: 1870 1943 2705 1870 Size: 73 762 4545 Fragments arranged by size: 4545 762 73 BamHI G'GATC_C Cuts at: 2710 3077 2710 Size: 367 5013 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2025 2701 4156 618 Size: 325 217 627 238 676 1455 1842 Fragments arranged by size: 1842 1455 676 627 325 238 217 BanII G_rGCy'C Cuts at: 1391 2036 2699 1391 Size: 645 663 4072 Fragments arranged by size: 4072 663 645 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4409 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3571 3605 1903 Size: 34 1634 34 3678 Fragments arranged by size: 3678 1634 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3272 4482 3272 Size: 1210 4170 BclI T'GATC_A Cuts at: 1548 1548 Size: 5380 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3991 136 Size: 107 122 71 631 605 2319 1525 Fragments arranged by size: 2319 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5375 5375 Size: 5380 BlpI GC'TnA_GC Cuts at: 2797 2797 Size: 5380 Bme1580I G_kGCm'C Cuts at: 1790 3441 4597 1790 Size: 1651 1156 2573 Fragments arranged by size: 2573 1651 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5380 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2085 2263 3111 Size: 416 53 83 399 24 668 178 848 Cuts at: 3111 4069 442 Size: 958 1753 Fragments arranged by size: 1753 958 848 668 416 399 178 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5380 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2168 2275 4040 1239 Size: 929 107 1765 2579 Fragments arranged by size: 2579 1765 929 107 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2265 3505 4370 4668 2265 Size: 1240 865 298 2977 Fragments arranged by size: 2977 1240 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4043 882 Size: 617 2544 2219 Fragments arranged by size: 2544 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2517 2554 3171 493 Size: 711 1313 37 617 2702 Fragments arranged by size: 2702 1313 711 617 37 BsaBI GATnn'nnATC Cuts at: 5374 5374 Size: 5380 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3187 3569 4204 275 Size: 53 83 186 936 1654 382 635 1451 Fragments arranged by size: 1654 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3813 4211 4485 4632 5043 3813 Size: 398 274 147 411 4150 Fragments arranged by size: 4150 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5155 Fragments arranged by size: 5155 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2258 2549 1186 Size: 1072 291 4017 Fragments arranged by size: 4017 1072 291 BseYI C'CCAG_C Cuts at: 2038 2225 4583 5079 2038 Size: 187 2358 496 2339 Fragments arranged by size: 2358 2339 496 187 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2508 1163 Size: 573 772 4035 Fragments arranged by size: 4035 772 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2830 3591 4619 1540 Size: 275 186 829 761 1028 2301 Fragments arranged by size: 2301 1028 829 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3441 3526 4597 3441 Size: 85 1071 4224 Fragments arranged by size: 4224 1071 85 BsmI GAATG_Cn' Cuts at: 2891 2984 2891 Size: 93 5287 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2036 2699 3441 3526 4597 1391 Size: 399 246 663 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 663 399 246 85 BspEI T'CCGG_A Cuts at: 5043 5043 Size: 5380 BspHI T'CATG_A Cuts at: 2481 2646 3269 4999 2481 Size: 165 623 1730 2862 Fragments arranged by size: 2862 1730 623 165 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2013 2182 2735 844 Size: 1169 169 553 3489 Fragments arranged by size: 3489 1169 553 169 BsrBI CCG'CTC Cuts at: 1394 1823 3267 1394 Size: 429 1444 3507 Fragments arranged by size: 3507 1444 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3875 4049 65 Size: 1183 2627 174 1396 Fragments arranged by size: 2627 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2133 4024 4211 1700 Size: 110 323 1891 187 2869 Fragments arranged by size: 2869 1891 323 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5380 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5380 BssSI C'ACGA_G Cuts at: 3440 4452 3440 Size: 1012 4368 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5380 BstBI TT'CG_AA Cuts at: 4184 4184 Size: 5380 BstEII G'GTnAC_C Cuts at: 1184 2599 1184 Size: 1415 3965 BstYI r'GATC_y Cuts at: 1972 2710 3077 3462 3479 4931 5017 5029 5375 Size: 738 367 385 17 1452 86 12 346 Cuts at: 5375 1972 Size: 1977 Fragments arranged by size: 1977 1452 738 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2139 2139 Size: 5380 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4828 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4641 Fragments arranged by size: 4641 683 56 BtsI GCAGTG_nn' Cuts at: 2896 3690 3710 2896 Size: 794 20 4566 Fragments arranged by size: 4566 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5380 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5345 DraI TTT'AAA Cuts at: 2684 2684 Size: 5380 DraIII CAC_nnn'GTG Cuts at: 2127 2127 Size: 5380 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4387 809 Size: 507 3071 1802 Fragments arranged by size: 3071 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2827 3716 5345 Size: 54 1425 50 275 18 997 889 1629 Cuts at: 5345 8 Size: 43 Fragments arranged by size: 1629 1425 997 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2827 1537 Size: 275 1015 4090 Fragments arranged by size: 4090 1015 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2318 3310 1353 Size: 965 992 3423 Fragments arranged by size: 3423 992 965 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3967 4341 4487 387 Size: 1064 138 2378 374 146 1280 Fragments arranged by size: 2378 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2168 2275 3443 4040 4827 5095 1239 Size: 393 536 107 1168 597 787 268 1524 Fragments arranged by size: 1524 1168 787 597 536 393 268 107 EcoNI CCTnn'n_nnAGG Cuts at: 2441 2441 Size: 5380 EcoO109I rG'GnC_Cy Cuts at: 2194 2539 2194 Size: 345 5035 EcoRI G'AATT_C Cuts at: 2612 2689 3067 2612 Size: 77 378 4925 Fragments arranged by size: 4925 378 77 FspI TGC'GCA Cuts at: 3886 3886 Size: 5380 HaeII r_GCGC'y Cuts at: 1678 4527 1678 Size: 2849 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4021 4053 4095 4127 1373 Size: 32 135 32 2449 32 42 32 2626 Fragments arranged by size: 2626 2449 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2724 2970 669 Size: 988 281 786 246 3079 Fragments arranged by size: 3079 988 786 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5380 HpaI GTT'AAC Cuts at: 2970 2970 Size: 5380 KpnI G_GTAC'C Cuts at: 2705 2705 Size: 5380 MfeI C'AATT_G Cuts at: 2979 2979 Size: 5380 MluI A'CGCG_T Cuts at: 5256 5256 Size: 5380 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4494 4678 1305 Size: 3189 184 2007 Fragments arranged by size: 3189 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3558 Fragments arranged by size: 3558 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3338 3697 3856 518 Size: 648 2172 359 159 2042 Fragments arranged by size: 2172 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2038 2068 2155 2229 2796 3473 4621 4866 Size: 432 30 87 74 567 677 1148 245 Cuts at: 4866 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 567 432 245 87 74 30 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4828 NdeI CA'TA_TG Cuts at: 387 387 Size: 5380 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5380 NotI GC'GGCC_GC Cuts at: 2827 2827 Size: 5380 NspI r_CATG'y Cuts at: 1132 1229 2738 4283 1132 Size: 97 1509 1545 2229 Fragments arranged by size: 2229 1545 1509 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5380 PciI A'CATG_T Cuts at: 4279 4279 Size: 5380 PflMI CCAn_nnn'nTGG Cuts at: 1108 2575 1108 Size: 1467 3913 PfoI T'CCnGG_A Cuts at: 2529 2529 Size: 5380 PmeI GTTT'AAAC Cuts at: 2684 2684 Size: 5380 PmlI CAC'GTG Cuts at: 2554 2554 Size: 5380 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3449 3481 4962 4994 3449 Size: 32 1481 32 3835 Fragments arranged by size: 3835 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2194 2194 Size: 5380 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5380 PsiI TTA'TAA Cuts at: 2950 2950 Size: 5380 PstI C_TGCA'G Cuts at: 830 2160 2732 830 Size: 1330 572 3478 Fragments arranged by size: 3478 1330 572 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5380 PvuII CAG'CTG Cuts at: 2068 2155 2068 Size: 87 5293 SalI G'TCGA_C Cuts at: 1936 2722 1936 Size: 786 4594 SbfI CC_TGCA'GG Cuts at: 2732 2732 Size: 5380 ScaI AGT'ACT Cuts at: 3628 5340 3628 Size: 1712 3668 SfcI C'TryA_G Cuts at: 826 1045 2156 2397 2728 3863 4544 4735 826 Size: 219 1111 241 331 1135 681 191 1471 Fragments arranged by size: 1471 1135 1111 681 331 241 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5380 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5380 SmaI CCC'GGG Cuts at: 1872 2707 1872 Size: 835 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2280 3484 4385 4647 820 Size: 197 926 337 1204 901 262 1553 Fragments arranged by size: 1553 1204 926 901 337 262 197 SnaBI TAC'GTA Cuts at: 493 3171 493 Size: 2678 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5380 SphI G_CATG'C Cuts at: 1132 2738 1132 Size: 1606 3774 SspI AAT'ATT Cuts at: 5 52 3304 5 Size: 47 3252 2081 Fragments arranged by size: 3252 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5380 StyI C'CwwG_G Cuts at: 513 1065 2460 2543 2819 513 Size: 552 1395 83 276 3074 Fragments arranged by size: 3074 1395 552 276 83 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2335 3407 3424 3577 3762 4207 325 Size: 1255 755 1072 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1072 755 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2511 3626 5338 Size: 274 80 33 51 1443 534 1115 1712 Cuts at: 5338 96 Size: 138 Fragments arranged by size: 1712 1443 1115 534 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5340 5372 5340 Size: 32 5348 Tth111I GACn'n_nGTC Cuts at: 1316 2077 2672 1316 Size: 761 595 4024 Fragments arranged by size: 4024 761 595 XbaI T'CTAG_A Cuts at: 2716 2716 Size: 5380 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2072 2072 Size: 5380 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5380 XmaI C'CCGG_G Cuts at: 1870 2705 1870 Size: 835 4545 XmnI GAAnn'nnTTC Cuts at: 3509 3509 Size: 5380 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3188 276 Size: 53 83 186 936 1654 2468 Fragments arranged by size: 2468 1654 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI