Restriction map for
pFN21AB8300
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# NO CUT ENZYMES ( 121 enzymes) #
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Acc65I AccIII AclI AcsI AflII
AflIII AgeI AhdI AocI Aor51HI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AvaI AviII AvrII
BamHI BbrPI BbvCI BcgI BciVI
BfaI BfrI BlnI BlpI Bpu1102I
BsaAI BseAI BsiWI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HincII HindII HindIII HpaI
KpnI KspI MaeI MfeI MluI
MroI MseI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tru9I
Tsp509I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AccI 1285 GTCTAC GT^[AC][GT]AC
Alw44I 715 GTGCAC G^TGCAC
AlwNI 274 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 715 GTGCAC G^TGCAC
BbeI 960 GGCGCC GGCGC^C
BbsI 246 GAAGAC GAAGAC(2/6)
BglII 602 AGATCT A^GATCT
BpuAI 246 GAAGAC GAAGAC(2/6)
BsaBI 576 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1118 TCCGGT [AT]^CCGG[AT]
BsiEI 207 CGGTCG CG[GA][CT]^CG
BsrI 90 ACTGG ACTGG(1/-1)
BssSI 733 CACGAG CACGAG(-5/-1)
BstAPI 1115 GCATCCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 718 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 543 CCTGACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
KasI 960 GGCGCC G^GCGCC
MamI 576 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 310 GCCGGC GCC^GGC
NarI 960 GGCGCC GG^CGCC
NgoMIV 310 GCCGGC G^CCGGC
NspI 905 ACATGC [GA]CATG^[CT]
PflMI 903 CCACATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 691 GAGCTC GAGCT^C
SapI 777 GCTCTTC GCTCTTC(1/4)
SfcI 1083 CTACAG C^T[GA][CT]AG
SfoI 960 GGCGCC GGC^GCC
SmlI 487 CTCAAG C^T[CT][GA]AG
StuI 362 AGGCCT AGG^CCT
Van91I 903 CCACATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 26 enzymes) #
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AatII 742 GACGTC GACGT^C
1188 GACGTC
ApaI 282 GGGCCC GGGCC^C
941 GGGCCC
AspHI 691 GAGCTC G[AT]GC[AT]^C
715 GTGCAC
AspI 553 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
895 GACGAGGTC
BalI 228 TGGCCA TGG^CCA
977 TGGCCA
BbvI 1059 GCTGC GCAGC(8/12)
1222 GCTGC
BclI 575 TGATCA T^GATCA
971 TGATCA
BsaI 505 GAGACC GGTCTC(1/5)
1372 GAGACC
BseRI 397 GAGGAG GAGGAG(10/8)
853 GAGGAG
BsiHKAI 691 GAGCTC G[AT]GC[AT]^C
715 GTGCAC
BspMI 1046 ACCTGC ACCTGC(4/8)
1263 GCAGGT
BsrBI 618 CCGCTC CCGCTC(-3/-3)
1311 CCGCTC
DdeI 783 CTCAG C^T[ACGT]AG
842 CTGAG
FbaI 575 TGATCA T^GATCA
971 TGATCA
HphI 787 GGTGA GGTGA(8/7)
578 TCACC
MluNI 228 TGGCCA TGG^CCA
977 TGGCCA
MscI 228 TGGCCA TGG^CCA
977 TGGCCA
MspA1I 145 CAGCTG C[AC]G^C[GT]G
1220 CAGCTG
NcoI 260 CCATGG C^CATGG
1376 CCATGG
PflFI 553 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
895 GACGAGGTC
PleI 538 GAGTC GAGTC(4/5)
1306 GAGTC
PvuII 145 CAGCTG CAG^CTG
1220 CAGCTG
TfiI 62 GAATC G^A[AT]TC
1001 GAATC
TseI 1059 GCTGC G^C[AT]GC
1222 GCTGC
Tth111I 553 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
895 GACGAGGTC
XcmI 509 CCATCTACGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1040 CCATAGACCTGCTGG
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# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AvaII BanI BanII BcnI
BglI BmyI BpmI BsaHI BsaJI
BsgI BsiYI BslI BsmAI BsmBI
BsmFI Bsp1286I BsrFI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr10I Cfr13I DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MnlI MslI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TaqI Tsp45I TspRI TthHB8I
XhoII