(Circular) (Six-base) MAPSORT of: pFN21AB8300.seq Check: 670 from: 1 to: 6094 pFN21AB8300 6094 bp With 182 enzymes: SgfI * February 27, 2009 14:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3259 3259 Size: 6094 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2751 3197 3904 278 Size: 53 83 186 936 1215 446 707 2468 Fragments arranged by size: 2468 1215 936 707 446 186 83 53 AccI GT'mk_AC Cuts at: 1937 3291 3437 1937 Size: 1354 146 4594 Fragments arranged by size: 4594 1354 146 Acc65I G'GTAC_C Cuts at: 3415 3415 Size: 6094 AclI AA'CG_TT Cuts at: 1563 4221 4594 1563 Size: 2658 373 3063 Fragments arranged by size: 3063 2658 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4157 5541 5809 1632 Size: 2525 1384 268 1917 Fragments arranged by size: 2525 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5897 AflIII A'CryG_T Cuts at: 1203 4993 5970 1203 Size: 3790 977 1327 Fragments arranged by size: 3790 1327 977 AgeI A'CCGG_T Cuts at: 4925 4925 Size: 6094 AhdI GACnn_n'nnGTC Cuts at: 4823 4823 Size: 6094 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6094 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2850 1184 Size: 1666 4428 AlwNI CAG_nnn'CTG Cuts at: 2284 3837 5409 2284 Size: 1553 1572 2969 Fragments arranged by size: 2969 1572 1553 ApaI G_GGCC'C Cuts at: 2291 2950 2291 Size: 659 5435 ApaLI G'TGCA_C Cuts at: 2720 4151 5307 2720 Size: 1431 1156 3507 Fragments arranged by size: 3507 1431 1156 ApoI r'AATT_y Cuts at: 1493 3403 3629 3781 5890 1493 Size: 1910 226 152 2109 1697 Fragments arranged by size: 2109 1910 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4648 160 Size: 872 3616 1606 Fragments arranged by size: 3616 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6094 AvaI C'yCGr_G Cuts at: 1870 1943 3419 1870 Size: 73 1476 4545 Fragments arranged by size: 4545 1476 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3249 3282 3249 Size: 33 6061 BamHI G'GATC_C Cuts at: 3424 3791 3424 Size: 367 5727 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2072 2528 2965 3014 3415 Size: 325 217 627 285 456 437 49 401 Cuts at: 3415 4870 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 456 437 401 325 285 217 49 BanII G_rGCy'C Cuts at: 1391 2291 2471 2663 2700 2950 3032 3413 1391 Size: 900 180 192 37 250 82 381 4072 Fragments arranged by size: 4072 900 381 250 192 180 82 37 BbeI G_GCGC'C Cuts at: 2969 2969 Size: 6094 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2258 928 Size: 971 359 4764 Fragments arranged by size: 4764 971 359 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4285 4319 1903 Size: 34 2348 34 3678 Fragments arranged by size: 3678 2348 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3986 5196 3986 Size: 1210 4884 BclI T'GATC_A Cuts at: 1548 2580 2976 1548 Size: 1032 396 4666 Fragments arranged by size: 4666 1032 396 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2216 2241 2843 Size: 107 122 71 631 605 544 25 602 Cuts at: 2843 3036 4705 136 Size: 193 1669 1525 Fragments arranged by size: 1669 1525 631 605 602 544 193 122 107 71 25 BglII A'GATC_T Cuts at: 2607 6089 2607 Size: 3482 2612 BlpI GC'TnA_GC Cuts at: 3511 3511 Size: 6094 Bme1580I G_kGCm'C Cuts at: 1790 2077 2246 2291 2531 2687 2724 2950 3017 Size: 287 169 45 240 156 37 226 67 Cuts at: 3017 4155 5311 1790 Size: 1138 1156 2573 Fragments arranged by size: 2573 1156 1138 287 240 226 169 156 67 45 37 BmgBI CAC'GTC Cuts at: 1318 2578 1318 Size: 1260 4834 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2105 3825 4783 Size: 416 53 83 399 24 688 1720 958 Cuts at: 4783 442 Size: 1753 Fragments arranged by size: 1753 1720 958 688 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6094 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2947 3334 2947 Size: 387 5707 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2204 2357 2684 2904 2972 3209 4754 1239 Size: 965 153 327 220 68 237 1545 2579 Fragments arranged by size: 2579 1545 965 327 237 220 153 68 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2477 4219 5084 5382 2477 Size: 1742 865 298 3189 Fragments arranged by size: 3189 1742 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2504 3371 4757 882 Size: 617 1005 867 1386 2219 Fragments arranged by size: 2219 1386 1005 867 617 BsaAI yAC'GTr Cuts at: 493 1204 3885 493 Size: 711 2681 2702 Fragments arranged by size: 2702 2681 711 BsaBI GATnn'nnATC Cuts at: 2585 6088 2585 Size: 3503 2591 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2748 2966 3159 3194 Size: 53 83 186 936 1215 218 193 35 Cuts at: 3194 3348 3901 4283 4918 275 Size: 154 553 382 635 1451 Fragments arranged by size: 1451 1215 936 635 553 382 218 193 186 154 83 53 35 BsaWI w'CCGG_w Cuts at: 3123 4527 4925 5199 5346 5757 3123 Size: 1404 398 274 147 411 3460 Fragments arranged by size: 3460 1404 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2795 2825 1178 Size: 30 165 30 1392 30 4447 Fragments arranged by size: 4447 1392 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2417 2873 1186 Size: 1231 456 4407 Fragments arranged by size: 4407 1231 456 BseYI C'CCAG_C Cuts at: 5297 5793 5297 Size: 496 5598 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2699 2720 3287 1163 Size: 573 963 21 567 3970 Fragments arranged by size: 3970 963 573 567 21 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2215 3544 4305 5333 1540 Size: 275 186 214 1329 761 1028 2301 Fragments arranged by size: 2301 1329 1028 761 275 214 186 BsiHKAI G_wGCw'C Cuts at: 2700 2724 4155 4240 5311 2700 Size: 24 1431 85 1071 3483 Fragments arranged by size: 3483 1431 1071 85 24 BsmI GAATG_Cn' Cuts at: 3605 3698 3605 Size: 93 6001 BsmBI CGTCTCn'nnnn_ Cuts at: 2399 3079 3185 2399 Size: 680 106 5308 Fragments arranged by size: 5308 680 106 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2077 2246 2291 2471 2531 2663 2687 Size: 399 287 169 45 180 60 132 24 Cuts at: 2687 2700 2724 2950 3017 3032 3413 4155 4240 Size: 13 24 226 67 15 381 742 85 Cuts at: 4240 5311 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 381 287 226 180 169 132 85 67 60 45 24 24 15 13 BspEI T'CCGG_A Cuts at: 5757 5757 Size: 6094 BspHI T'CATG_A Cuts at: 3983 5713 3983 Size: 1730 4364 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3060 3259 3449 844 Size: 2216 199 190 3489 Fragments arranged by size: 3489 2216 199 190 BsrBI CCG'CTC Cuts at: 1394 1823 2625 3318 3981 1394 Size: 429 802 693 663 3507 Fragments arranged by size: 3507 802 693 663 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4589 4763 65 Size: 1183 3341 174 1396 Fragments arranged by size: 3341 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2210 2315 3060 4738 4925 1700 Size: 110 400 105 745 1678 187 2869 Fragments arranged by size: 2869 1678 745 400 187 110 105 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6094 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6094 BssSI C'ACGA_G Cuts at: 2738 4154 5166 2738 Size: 1416 1012 3666 Fragments arranged by size: 3666 1416 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3126 794 Size: 2332 3762 BstBI TT'CG_AA Cuts at: 4898 4898 Size: 6094 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6094 BstXI CCAn_nnnn'nTGG Cuts at: 2266 2272 2506 2266 Size: 6 234 5854 Fragments arranged by size: 5854 234 6 BstYI r'GATC_y Cuts at: 1972 2040 2436 2607 3111 3424 3791 4176 4193 Size: 68 396 171 504 313 367 385 17 Cuts at: 4193 5645 5731 5743 6089 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 504 396 385 367 346 313 171 86 68 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2265 2992 3381 513 Size: 552 939 261 727 389 3226 Fragments arranged by size: 3226 939 727 552 389 261 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2245 2296 487 Size: 683 56 1019 51 4285 Fragments arranged by size: 4285 1019 683 56 51 BtsI GCAGTG_nn' Cuts at: 2326 2687 3197 3610 4404 4424 2326 Size: 361 510 413 794 20 3996 Fragments arranged by size: 3996 794 510 413 361 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6094 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6059 DraI TTT'AAA Cuts at: 3398 3398 Size: 6094 DraIII CAC_nnn'GTG Cuts at: 2728 2728 Size: 6094 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5101 809 Size: 507 3785 1802 Fragments arranged by size: 3785 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2233 2256 2982 Size: 54 1425 50 275 18 403 23 726 Cuts at: 2982 3541 4430 6059 8 Size: 559 889 1629 43 Fragments arranged by size: 1629 1425 889 726 559 403 275 54 50 43 23 18 EagI C'GGCC_G Cuts at: 1537 1812 3541 1537 Size: 275 1729 4090 Fragments arranged by size: 4090 1729 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2789 3305 3362 4024 1353 Size: 1436 516 57 662 3423 Fragments arranged by size: 3423 1436 662 516 57 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2885 4681 5055 5201 387 Size: 1064 138 1296 1796 374 146 1280 Fragments arranged by size: 1796 1296 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2698 2698 Size: 6094 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2204 2357 2684 2904 2972 3209 4157 Size: 393 572 153 327 220 68 237 948 Cuts at: 4157 4754 5541 5809 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 948 787 597 572 393 327 268 237 220 153 68 EcoNI CCTnn'n_nnAGG Cuts at: 2552 2552 Size: 6094 EcoO109I rG'GnC_Cy Cuts at: 2281 2288 2947 2281 Size: 7 659 5428 Fragments arranged by size: 5428 659 7 EcoRI G'AATT_C Cuts at: 3403 3781 3403 Size: 378 5716 FspI TGC'GCA Cuts at: 4600 4600 Size: 6094 HaeII r_GCGC'y Cuts at: 1678 2360 2783 2969 5241 1678 Size: 682 423 186 2272 2531 Fragments arranged by size: 2531 2272 682 423 186 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2197 2229 2795 2827 4735 Size: 32 135 32 625 32 566 32 1908 Cuts at: 4735 4767 4809 4841 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1908 625 566 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3438 3684 669 Size: 988 281 1500 246 3079 Fragments arranged by size: 3079 1500 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6094 HpaI GTT'AAC Cuts at: 3684 3684 Size: 6094 KasI G'GCGC_C Cuts at: 2965 2965 Size: 6094 KpnI G_GTAC'C Cuts at: 3419 3419 Size: 6094 MfeI C'AATT_G Cuts at: 3693 3693 Size: 6094 MluI A'CGCG_T Cuts at: 5970 5970 Size: 6094 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5208 5392 1305 Size: 3903 184 2007 Fragments arranged by size: 3903 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2235 2984 10 Size: 54 1425 343 403 749 3120 Fragments arranged by size: 3120 1425 749 403 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2264 2270 2504 2534 4052 4411 4570 Size: 648 1098 6 234 30 1518 359 159 Cuts at: 4570 518 Size: 2042 Fragments arranged by size: 2042 1518 1098 648 359 234 159 30 6 MspA1I CmG'CkG Cuts at: 1606 2152 3227 3510 4187 5335 5580 1606 Size: 546 1075 283 677 1148 245 2120 Fragments arranged by size: 2120 1148 1075 677 546 283 245 NaeI GCC'GGC Cuts at: 2317 2317 Size: 6094 NarI GG'CG_CC Cuts at: 2966 2966 Size: 6094 NcoI C'CATG_G Cuts at: 513 1065 2004 2265 3381 513 Size: 552 939 261 1116 3226 Fragments arranged by size: 3226 1116 939 552 261 NdeI CA'TA_TG Cuts at: 387 387 Size: 6094 NgoMIV G'CCGG_C Cuts at: 2315 2315 Size: 6094 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6094 NotI GC'GGCC_GC Cuts at: 3541 3541 Size: 6094 NspI r_CATG'y Cuts at: 1132 1229 2914 3452 4997 1132 Size: 97 1685 538 1545 2229 Fragments arranged by size: 2229 1685 1545 538 97 PasI CC'CwG_GG Cuts at: 1348 2284 2767 1348 Size: 936 483 4675 Fragments arranged by size: 4675 936 483 PciI A'CATG_T Cuts at: 4993 4993 Size: 6094 PflMI CCAn_nnn'nTGG Cuts at: 1108 2914 1108 Size: 1806 4288 PfoI T'CCnGG_A Cuts at: 2034 2193 3303 2034 Size: 159 1110 4825 Fragments arranged by size: 4825 1110 159 PmeI GTTT'AAAC Cuts at: 3398 3398 Size: 6094 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4163 4195 5676 5708 4163 Size: 32 1481 32 4549 Fragments arranged by size: 4549 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6094 PsiI TTA'TAA Cuts at: 3664 3664 Size: 6094 PspOMI G'GGCC_C Cuts at: 2287 2946 2287 Size: 659 5435 PstI C_TGCA'G Cuts at: 830 3446 830 Size: 2616 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6094 PvuII CAG'CTG Cuts at: 2152 3227 2152 Size: 1075 5019 SacI G_AGCT'C Cuts at: 2700 2700 Size: 6094 SalI G'TCGA_C Cuts at: 1936 3436 1936 Size: 1500 4594 SapI GCTCTTCn'nnn_ Cuts at: 2789 2789 Size: 6094 SbfI CC_TGCA'GG Cuts at: 3446 3446 Size: 6094 ScaI AGT'ACT Cuts at: 4342 6054 4342 Size: 1712 4382 SfcI C'TryA_G Cuts at: 826 1045 3088 3442 4577 5258 5449 826 Size: 219 2043 354 1135 681 191 1471 Fragments arranged by size: 2043 1471 1135 681 354 219 191 SfoI GGC'GCC Cuts at: 2967 2967 Size: 6094 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6094 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6094 SmaI CCC'GGG Cuts at: 1872 3421 1872 Size: 1549 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2492 4198 5099 5361 820 Size: 197 926 549 1706 901 262 1553 Fragments arranged by size: 1706 1553 926 901 549 262 197 SnaBI TAC'GTA Cuts at: 493 3885 493 Size: 3392 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6094 SphI G_CATG'C Cuts at: 1132 3452 1132 Size: 2320 3774 SspI AAT'ATT Cuts at: 5 52 4018 5 Size: 47 3966 2081 Fragments arranged by size: 3966 2081 47 StuI AGG'CCT Cuts at: 1517 2369 1517 Size: 852 5242 StyI C'CwwG_G Cuts at: 513 1065 2004 2112 2265 2472 2553 2838 3381 Size: 552 939 108 153 207 81 285 543 Cuts at: 3381 3533 513 Size: 152 3074 Fragments arranged by size: 3074 939 552 543 285 207 153 152 108 81 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2366 2780 4121 4138 4291 4476 4921 Size: 1255 786 414 1341 17 153 185 445 Cuts at: 4921 325 Size: 1498 Fragments arranged by size: 1498 1341 1255 786 445 414 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3327 4340 6052 Size: 274 80 33 51 1443 1350 1013 1712 Cuts at: 6052 96 Size: 138 Fragments arranged by size: 1712 1443 1350 1013 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2714 2746 6054 6086 2714 Size: 32 3308 32 2722 Fragments arranged by size: 3308 2722 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2561 2903 1316 Size: 1245 342 4507 Fragments arranged by size: 4507 1245 342 XbaI T'CTAG_A Cuts at: 3430 3430 Size: 6094 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2521 3052 2521 Size: 531 5563 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6094 XmaI C'CCGG_G Cuts at: 1870 3419 1870 Size: 1549 4545 XmnI GAAnn'nnTTC Cuts at: 4223 4223 Size: 6094 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2749 3195 3902 276 Size: 53 83 186 936 1215 446 707 2468 Fragments arranged by size: 2468 1215 936 707 446 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI