Restriction map for
pFN21AB8300
(Linear) (Six-base) MAPSORT of: KIBB8300.seq Check: 3715 from: 1 to: 1389
KIBB8300.orf
With 182 enzymes: SgfI *
March 3, 2008 11:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1254 1389
Size: 1254 135
AatII G_ACGT'C
Cuts at: 0 746 1192 1389
Size: 746 446 197
Fragments arranged by size:
746 446 197
AccI GT'mk_AC
Cuts at: 0 1286 1389
Size: 1286 103
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 845 1389
Size: 845 544
AlwNI CAG_nnn'CTG
Cuts at: 0 279 1389
Size: 279 1110
ApaI G_GGCC'C
Cuts at: 0 286 945 1389
Size: 286 659 444
Fragments arranged by size:
659 444 286
ApaLI G'TGCA_C
Cuts at: 0 715 1389
Size: 715 674
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1244 1277 1389
Size: 1244 33 112
Fragments arranged by size:
1244 112 33
BanI G'GyrC_C
Cuts at: 0 67 523 960 1009 1389
Size: 67 456 437 49 380
Fragments arranged by size:
456 437 380 67 49
BanII G_rGCy'C
Cuts at: 0 286 466 658 695 945 1027 1389
Size: 286 180 192 37 250 82 362
Fragments arranged by size:
362 286 250 192 180 82 37
BbeI G_GCGC'C
Cuts at: 0 964 1389
Size: 964 425
BbsI GAAGACnn'nnnn_
Cuts at: 0 253 1389
Size: 253 1136
BclI T'GATC_A
Cuts at: 0 575 971 1389
Size: 575 396 418
Fragments arranged by size:
575 418 396
BglI GCCn_nnn'nGGC
Cuts at: 0 211 236 838 1031 1389
Size: 211 25 602 193 358
Fragments arranged by size:
602 358 211 193 25
BglII A'GATC_T
Cuts at: 0 602 1389
Size: 602 787
Bme1580I G_kGCm'C
Cuts at: 0 72 241 286 526 682 719 945 1012
Size: 72 169 45 240 156 37 226 67
Cuts at: 1012 1389
Size: 377
Fragments arranged by size:
377 240 226 169 156 72 67 45
37
BmgBI CAC'GTC
Cuts at: 0 573 1389
Size: 573 816
BmrI ACTGGGnnnn_n'
Cuts at: 0 100 1389
Size: 100 1289
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 942 1329 1389
Size: 942 387 60
Fragments arranged by size:
942 387 60
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 352 679 899 967 1204 1389
Size: 199 153 327 220 68 237 185
Fragments arranged by size:
327 237 220 199 185 153 68
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 472 1389
Size: 472 917
BsaI GGTCTCn'nnnn_
Cuts at: 0 499 1366 1389
Size: 499 867 23
Fragments arranged by size:
867 499 23
BsaBI GATnn'nnATC
Cuts at: 0 580 1389
Size: 580 809
BsaHI Gr'CG_yC
Cuts at: 0 743 961 1154 1189 1343 1389
Size: 743 218 193 35 154 46
Fragments arranged by size:
743 218 193 154 46 35
BsaWI w'CCGG_w
Cuts at: 0 1118 1389
Size: 1118 271
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 790 820 1389
Size: 790 30 569
Fragments arranged by size:
790 569 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 412 868 1389
Size: 412 456 521
Fragments arranged by size:
521 456 412
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 694 715 1282 1389
Size: 694 21 567 107
Fragments arranged by size:
694 567 107 21
BsiEI CG_ry'CG
Cuts at: 0 210 1389
Size: 210 1179
BsiHKAI G_wGCw'C
Cuts at: 0 695 719 1389
Size: 695 24 670
Fragments arranged by size:
695 670 24
BsmBI CGTCTCn'nnnn_
Cuts at: 0 394 1074 1180 1389
Size: 394 680 106 209
Fragments arranged by size:
680 394 209 106
Bsp1286I G_dGCh'C
Cuts at: 0 72 241 286 466 526 658 682 695
Size: 72 169 45 180 60 132 24 13
Cuts at: 695 719 945 1012 1027 1389
Size: 24 226 67 15 362
Fragments arranged by size:
362 226 180 169 132 72 67 60
45 24 24 15 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1055 1254 1389
Size: 1055 199 135
Fragments arranged by size:
1055 199 135
BsrBI CCG'CTC
Cuts at: 0 620 1313 1389
Size: 620 693 76
Fragments arranged by size:
693 620 76
BsrFI r'CCGG_y
Cuts at: 0 205 310 1055 1389
Size: 205 105 745 334
Fragments arranged by size:
745 334 205 105
BssSI C'ACGA_G
Cuts at: 0 733 1389
Size: 733 656
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1121 1389
Size: 1121 268
BstXI CCAn_nnnn'nTGG
Cuts at: 0 261 267 501 1389
Size: 261 6 234 888
Fragments arranged by size:
888 261 234 6
BstYI r'GATC_y
Cuts at: 0 35 431 602 1106 1389
Size: 35 396 171 504 283
Fragments arranged by size:
504 396 283 171 35
BtgI C'CryG_G
Cuts at: 0 260 987 1376 1389
Size: 260 727 389 13
Fragments arranged by size:
727 389 260 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 240 291 1389
Size: 240 51 1098
Fragments arranged by size:
1098 240 51
BtsI GCAGTG_nn'
Cuts at: 0 321 682 1192 1389
Size: 321 361 510 197
Fragments arranged by size:
510 361 321 197
DraIII CAC_nnn'GTG
Cuts at: 0 723 1389
Size: 723 666
EaeI y'GGCC_r
Cuts at: 0 228 251 977 1389
Size: 228 23 726 412
Fragments arranged by size:
726 412 228 23
EarI CTCTTCn'nnn_
Cuts at: 0 784 1300 1357 1389
Size: 784 516 57 32
Fragments arranged by size:
784 516 57 32
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 880 1389
Size: 880 509
EcoICRI GAG'CTC
Cuts at: 0 693 1389
Size: 693 696
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 199 352 679 899 967 1204 1389
Size: 199 153 327 220 68 237 185
Fragments arranged by size:
327 237 220 199 185 153 68
EcoNI CCTnn'n_nnAGG
Cuts at: 0 547 1389
Size: 547 842
EcoO109I rG'GnC_Cy
Cuts at: 0 276 283 942 1389
Size: 276 7 659 447
Fragments arranged by size:
659 447 276 7
HaeII r_GCGC'y
Cuts at: 0 355 778 964 1389
Size: 355 423 186 425
Fragments arranged by size:
425 423 355 186
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 192 224 790 822 1389
Size: 192 32 566 32 567
Fragments arranged by size:
567 566 192 32 32
KasI G'GCGC_C
Cuts at: 0 960 1389
Size: 960 429
MscI TGG'CCA
Cuts at: 0 230 979 1389
Size: 230 749 410
Fragments arranged by size:
749 410 230
MslI CAynn'nnrTG
Cuts at: 0 259 265 499 529 1389
Size: 259 6 234 30 860
Fragments arranged by size:
860 259 234 30 6
MspA1I CmG'CkG
Cuts at: 0 147 1222 1389
Size: 147 1075 167
Fragments arranged by size:
1075 167 147
NaeI GCC'GGC
Cuts at: 0 312 1389
Size: 312 1077
NarI GG'CG_CC
Cuts at: 0 961 1389
Size: 961 428
NcoI C'CATG_G
Cuts at: 0 260 1376 1389
Size: 260 1116 13
Fragments arranged by size:
1116 260 13
NgoMIV G'CCGG_C
Cuts at: 0 310 1389
Size: 310 1079
NspI r_CATG'y
Cuts at: 0 909 1389
Size: 909 480
PasI CC'CwG_GG
Cuts at: 0 279 762 1389
Size: 279 483 627
Fragments arranged by size:
627 483 279
PflMI CCAn_nnn'nTGG
Cuts at: 0 909 1389
Size: 909 480
PfoI T'CCnGG_A
Cuts at: 0 29 188 1298 1389
Size: 29 159 1110 91
Fragments arranged by size:
1110 159 91 29
PspOMI G'GGCC_C
Cuts at: 0 282 941 1389
Size: 282 659 448
Fragments arranged by size:
659 448 282
PvuII CAG'CTG
Cuts at: 0 147 1222 1389
Size: 147 1075 167
Fragments arranged by size:
1075 167 147
SacI G_AGCT'C
Cuts at: 0 695 1389
Size: 695 694
SapI GCTCTTCn'nnn_
Cuts at: 0 784 1389
Size: 784 605
SfcI C'TryA_G
Cuts at: 0 1083 1389
Size: 1083 306
SfoI GGC'GCC
Cuts at: 0 962 1389
Size: 962 427
SmlI C'TyrA_G
Cuts at: 0 487 1389
Size: 487 902
StuI AGG'CCT
Cuts at: 0 364 1389
Size: 364 1025
StyI C'CwwG_G
Cuts at: 0 107 260 467 548 833 1376 1389
Size: 107 153 207 81 285 543 13
Fragments arranged by size:
543 285 207 153 107 81 13
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 361 775 1389
Size: 361 414 614
Fragments arranged by size:
614 414 361
TatI w'GTAC_w
Cuts at: 0 1322 1389
Size: 1322 67
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 709 741 1389
Size: 709 32 648
Fragments arranged by size:
709 648 32
Tth111I GACn'n_nGTC
Cuts at: 0 556 898 1389
Size: 556 342 491
Fragments arranged by size:
556 491 342
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 516 1047 1389
Size: 516 531 342
Fragments arranged by size:
531 516 342
ZraI GAC'GTC
Cuts at: 0 744 1190 1389
Size: 744 446 199
Fragments arranged by size:
744 446 199
Enzymes that do cut:
AarI AatII AccI AlfI AlwNI ApaI ApaLI BaeI
BanI BanII BbeI BbsI BclI BglI BglII Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaBI BsaHI
BsaWI BsaXI BseRI BsgI BsiEI BsiHKAI BsmBI Bsp1286I
BspMI BsrBI BsrFI BssSI BstAPI BstXI BstYI BtgI
BtgZI BtsI DraIII EaeI EarI EciI EcoICRI Eco57MI
EcoNI EcoO109I HaeII Hin4I KasI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NspI PasI PflMI PfoI
PspOMI PvuII SacI SapI SfcI SfoI SmlI StuI
StyI TaqII TatI TstI Tth111I XcmI ZraI
Enzymes that do not cut:
Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI
AleI AloI ApoI AscI AseI AsiSI AvaI AvrII
BamHI BbvCI BcgI BciVI BfrBI BlpI BmtI Bpu10I
BsaAI BseYI BsiWI BsmI BspEI BspHI BsrDI BsrGI
BssHII BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI
DrdI EagI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KpnI MfeI MluI MmeI NdeI
NheI NotI NruI NsiI PacI PciI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PstI PvuI
RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI XbaI XhoI XmaI XmnI