Restriction map for
pFN21AB8299
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# NO CUT ENZYMES ( 129 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BanI BbeI BbrPI
BbvCI BcgI BciVI BclI BfrI
BglI BglII BlnI BsaAI BsaBI
BsaHI BseAI BsgI BsiEI BsiWI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrDI BsrGI BssHII Bst1107I BstAPI
BstBI BstUI BstZ17I ClaI CpoI
DraI EagI Eam1105I EclXI Eco47III
Eco52I EcoRI EcoRV EcoT22I FbaI
FspI HaeII Hin1I HincII HindII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MseI MunI
MvnI NarI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI SspBI SspI StuI
SwaI TaqI Tru9I Tsp509I Tth111I
TthHB8I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AlwI 669 GATCC GGATC(4/5)
AocI 1117 CCTGAGG CC^T[ACGT]AGG
BalI 1069 TGGCCA TGG^CCA
BanII 4 GAGCCC G[GA]GC[CT]^C
BbsI 727 GAAGAC GAAGAC(2/6)
BpuAI 727 GAAGAC GAAGAC(2/6)
BsaWI 125 ACCGGA [AT]^CCGG[AT]
BsmBI 31 GAGACG CGTCTC(1/5)
BsmI 1260 GAATGC GAATGC(1/-1)
BspHI 1157 TCATGA T^CATGA
BsrBI 655 GAGCGG CCGCTC(-3/-3)
BsrFI 1304 GCCGGC [GA]^CCGG[CT]
BstEII 1467 GGTGACC G^GT[ACGT]ACC
BstPI 1467 GGTGACC G^GT[ACGT]ACC
BstXI 1457 CCATGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 668 AGATCC [GA]^GATC[CT]
Bsu36I 1117 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1304 GCCGGC [GA]^CCGG[CT]
DrdI 289 GACATCTCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1117 CCTGAGG CC^T[ACGT]AGG
EcoNI 159 CCTGGAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1467 GGTGACC G^GT[ACGT]ACC
EcoT14I 662 CCATGG C^C[AT][AT]GG
FseI 1303 GGCCGGCC GGCCGG^CC
HindIII 1144 AAGCTT A^AGCTT
MflI 668 AGATCC [GA]^GATC[CT]
MluNI 1069 TGGCCA TGG^CCA
MscI 1069 TGGCCA TGG^CCA
MspA1I 541 CAGCTG C[AC]G^C[GT]G
NaeI 1304 GCCGGC GCC^GGC
NcoI 662 CCATGG C^CATGG
NgoMIV 1304 GCCGGC G^CCGGC
PflMI 1506 CCAGCCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 754 GGGACCC [GA]G^G[AT]CC[CT]
PvuII 541 CAGCTG CAG^CTG
RcaI 1157 TCATGA T^CATGA
SapI 681 GCTCTTC GCTCTTC(1/4)
ScaI 1319 AGTACT AGT^ACT
StyI 662 CCATGG C^C[AT][AT]GG
Van91I 1506 CCAGCCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 668 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 22 enzymes) #
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BlpI 375 GCTCAGC GC^T[ACGT]AGC
1002 GCTGAGC
Bpu1102I 375 GCTCAGC GC^T[ACGT]AGC
1002 GCTGAGC
BsaI 794 GAGACC GGTCTC(1/5)
150 GGTCTC
BssSI 1450 CACGAG CACGAG(-5/-1)
370 CTCGTG
CelII 375 GCTCAGC GC^T[ACGT]AGC
1002 GCTGAGC
CfoI 534 GCGC GCG^C
567 GCGC
DpnII 669 GATC ^GATC
780 GATC
DraII 754 GGGACCC [GA]G^G[ACGT]CC[CT]
1286 GGGGCCT
DraIII 769 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
820 CACGCAGTG
EaeI 816 CGGCCA [CT]^GGCC[GA]
1069 TGGCCA
EcoO109I 754 GGGACCC [GA]G^G[ACGT]CC[CT]
1286 GGGGCCT
HgaI 594 GACGC GACGC(5/10)
730 GACGC
HhaI 534 GCGC GCG^C
567 GCGC
HinP1I 534 GCGC G^CGC
567 GCGC
MaeII 100 ACGT A^CGT
1540 ACGT
MboI 669 GATC ^GATC
780 GATC
NdeII 669 GATC ^GATC
780 GATC
Sau3AI 669 GATC ^GATC
780 GATC
SbfI 264 CCTGCAGG CCTGCA^GG
1545 CCTGCAGG
SmlI 352 CTCAAG C^T[CT][GA]AG
850 CTCAAG
Sse8387I 264 CCTGCAGG CCTGCA^GG
1545 CCTGCAGG
XcmI 896 CCACGGAGTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1283 CCAGGGGCCTCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AfaI AluI AlwNI AspHI
AvaI AvaII BbvI BcnI BfaI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
Cac8I Cfr13I DdeI DsaI EarI
EcoRII FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboII MnlI
MslI MspI MvaI MwoI NciI
NlaIII NlaIV PleI PstI RsaI
Sau96I ScrFI SfaNI SfcI SmaI
TfiI TseI Tsp45I TspRI XmaCI
XmaI