(Circular) (Six-base) MAPSORT of: pFN21AB8299.seq Check: 2370 from: 1 to: 6316 pFN21AB8299 6316 bp With 182 enzymes: SgfI * February 27, 2009 14:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4126 278 Size: 53 83 186 936 2590 2468 Fragments arranged by size: 2590 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3659 1937 Size: 1722 4594 Acc65I G'GTAC_C Cuts at: 3637 3637 Size: 6316 AclI AA'CG_TT Cuts at: 1563 4443 4816 1563 Size: 2880 373 3063 Fragments arranged by size: 3063 2880 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2637 3005 3080 4379 5763 6031 1632 Size: 1005 368 75 1299 1384 268 1917 Fragments arranged by size: 1917 1384 1299 1005 368 268 75 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6119 AflIII A'CryG_T Cuts at: 1203 5215 6192 1203 Size: 4012 977 1327 Fragments arranged by size: 4012 1327 977 AgeI A'CCGG_T Cuts at: 5147 5147 Size: 6316 AhdI GACnn_n'nnGTC Cuts at: 5045 5045 Size: 6316 AleI CACnn'nnGTG Cuts at: 1166 2534 3182 1166 Size: 1368 648 4300 Fragments arranged by size: 4300 1368 648 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2299 2393 2552 1184 Size: 1115 94 159 4948 Fragments arranged by size: 4948 1115 159 94 AlwNI CAG_nnn'CTG Cuts at: 2269 2404 2548 2560 3091 4059 5631 2269 Size: 135 144 12 531 968 1572 2954 Fragments arranged by size: 2954 1572 968 531 144 135 12 ApaLI G'TGCA_C Cuts at: 4373 5529 4373 Size: 1156 5160 ApoI r'AATT_y Cuts at: 1493 3625 3851 4003 6112 1493 Size: 2132 226 152 2109 1697 Fragments arranged by size: 2132 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4870 160 Size: 872 3838 1606 Fragments arranged by size: 3838 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6316 AvaI C'yCGr_G Cuts at: 1870 1943 2013 2226 3314 3531 3641 1870 Size: 73 70 213 1088 217 110 4545 Fragments arranged by size: 4545 1088 217 213 110 73 70 BamHI G'GATC_C Cuts at: 3646 4013 3646 Size: 367 5949 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3637 5092 618 Size: 325 217 627 1850 1455 1842 Fragments arranged by size: 1850 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2013 3635 1391 Size: 622 1622 4072 Fragments arranged by size: 4072 1622 622 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2739 928 Size: 971 840 4505 Fragments arranged by size: 4505 971 840 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4507 4541 1903 Size: 34 2570 34 3678 Fragments arranged by size: 3678 2570 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4208 5418 4208 Size: 1210 5106 BclI T'GATC_A Cuts at: 1548 1548 Size: 6316 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4927 136 Size: 107 122 71 631 605 3255 1525 Fragments arranged by size: 3255 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6311 6311 Size: 6316 BlpI GC'TnA_GC Cuts at: 2381 3008 3733 2381 Size: 627 725 4964 Fragments arranged by size: 4964 725 627 Bme1580I G_kGCm'C Cuts at: 1790 2941 3031 3237 4377 5533 1790 Size: 1151 90 206 1140 1156 2573 Fragments arranged by size: 2573 1156 1151 1140 206 90 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6316 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4047 5005 442 Size: 416 53 83 399 24 2630 958 1753 Fragments arranged by size: 2630 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6316 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3015 3015 Size: 6316 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2369 2612 2817 2903 3478 4976 1239 Size: 1130 243 205 86 575 1498 2579 Fragments arranged by size: 2579 1498 1130 575 243 205 86 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2342 2840 4441 5306 5604 2342 Size: 498 1601 865 298 3054 Fragments arranged by size: 3054 1601 865 498 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2161 2793 4979 882 Size: 617 662 632 2186 2219 Fragments arranged by size: 2219 2186 662 632 617 BsaAI yAC'GTr Cuts at: 493 1204 4107 493 Size: 711 2903 2702 Fragments arranged by size: 2903 2702 711 BsaBI GATnn'nnATC Cuts at: 6310 6310 Size: 6316 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4123 4505 5140 275 Size: 53 83 186 936 2590 382 635 1451 Fragments arranged by size: 2590 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2130 4749 5147 5421 5568 5979 2130 Size: 2619 398 274 147 411 2467 Fragments arranged by size: 2619 2467 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2622 2652 3476 3506 1178 Size: 30 165 30 1219 30 824 30 3988 Fragments arranged by size: 3988 1219 824 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2060 2479 2495 2825 2864 2933 3419 3455 Size: 874 419 16 330 39 69 486 36 Cuts at: 3455 3580 1186 Size: 125 3922 Fragments arranged by size: 3922 874 486 419 330 125 69 39 36 16 BseYI C'CCAG_C Cuts at: 2602 3043 5519 6015 2602 Size: 441 2476 496 2903 Fragments arranged by size: 2903 2476 496 441 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5743 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3766 4527 5555 1540 Size: 275 186 1765 761 1028 2301 Fragments arranged by size: 2301 1765 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2091 2382 2688 3224 3456 4377 4462 5533 2091 Size: 291 306 536 232 921 85 1071 2874 Fragments arranged by size: 2874 1071 921 536 306 291 232 85 BsmI GAATG_Cn' Cuts at: 3271 3827 3920 3271 Size: 556 93 5667 Fragments arranged by size: 5667 556 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2030 2030 Size: 6316 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2013 2091 2382 2688 2941 3031 3224 Size: 399 223 78 291 306 253 90 193 Cuts at: 3224 3237 3456 3635 4377 4462 5533 1391 Size: 13 219 179 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 306 291 253 223 219 193 179 90 85 78 13 BspEI T'CCGG_A Cuts at: 5979 5979 Size: 6316 BspHI T'CATG_A Cuts at: 3162 4205 5935 3162 Size: 1043 1730 3543 Fragments arranged by size: 3543 1730 1043 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3671 844 Size: 2827 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2662 4203 1394 Size: 429 839 1541 3507 Fragments arranged by size: 3507 1541 839 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4811 4985 65 Size: 1183 3563 174 1396 Fragments arranged by size: 3563 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3309 4960 5147 1700 Size: 110 1499 1651 187 2869 Fragments arranged by size: 2869 1651 1499 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6316 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6316 BssSI C'ACGA_G Cuts at: 2375 3455 4376 5388 2375 Size: 1080 921 1012 3303 Fragments arranged by size: 3303 1080 1012 921 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6316 BstBI TT'CG_AA Cuts at: 5120 5120 Size: 6316 BstEII G'GTnAC_C Cuts at: 1184 3472 1184 Size: 2288 4028 BstXI CCAn_nnnn'nTGG Cuts at: 3469 3469 Size: 6316 BstYI r'GATC_y Cuts at: 1972 2673 3646 4013 4398 4415 5867 5953 5965 Size: 701 973 367 385 17 1452 86 12 Cuts at: 5965 6311 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 973 701 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3123 3123 Size: 6316 BtgI C'CryG_G Cuts at: 513 1065 2004 2031 2667 2818 2901 513 Size: 552 939 27 636 151 83 3928 Fragments arranged by size: 3928 939 636 552 151 83 27 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5577 Fragments arranged by size: 5577 683 56 BtsI GCAGTG_nn' Cuts at: 2835 3832 4626 4646 2835 Size: 997 794 20 4505 Fragments arranged by size: 4505 997 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6316 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6281 DraI TTT'AAA Cuts at: 3620 3620 Size: 6316 DraIII CAC_nnn'GTG Cuts at: 2779 2830 2779 Size: 51 6265 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2300 5323 809 Size: 507 984 3023 1802 Fragments arranged by size: 3023 1802 984 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2821 3074 3763 Size: 54 1425 50 275 18 991 253 689 Cuts at: 3763 4652 6281 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 991 889 689 275 253 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3763 1537 Size: 275 1951 4090 Fragments arranged by size: 4090 1951 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2129 2513 2693 3182 4246 1353 Size: 776 384 180 489 1064 3423 Fragments arranged by size: 3423 1064 776 489 384 180 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2159 3146 4903 5277 5423 387 Size: 1064 138 570 987 1757 374 146 1280 Fragments arranged by size: 1757 1280 1064 987 570 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2369 2612 2637 2817 2903 3005 3080 Size: 393 737 243 25 180 86 102 75 Cuts at: 3080 3478 4379 4976 5763 6031 1239 Size: 398 901 597 787 268 1524 Fragments arranged by size: 1524 901 787 737 597 398 393 268 243 180 102 86 75 25 EcoNI CCTnn'n_nnAGG Cuts at: 2168 2168 Size: 6316 EcoO109I rG'GnC_Cy Cuts at: 2760 3292 2760 Size: 532 5784 EcoRI G'AATT_C Cuts at: 3625 4003 3625 Size: 378 5938 FseI GG_CCGG'CC Cuts at: 3313 3313 Size: 6316 FspI TGC'GCA Cuts at: 4822 4822 Size: 6316 HaeII r_GCGC'y Cuts at: 1678 5463 1678 Size: 3785 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3476 3508 3587 3619 4957 Size: 32 135 32 1904 32 79 32 1338 Cuts at: 4957 4989 5031 5063 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1904 1338 135 79 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3660 3906 669 Size: 988 281 1722 246 3079 Fragments arranged by size: 3079 1722 988 281 246 HindIII A'AGCT_T Cuts at: 748 3149 748 Size: 2401 3915 HpaI GTT'AAC Cuts at: 3906 3906 Size: 6316 KpnI G_GTAC'C Cuts at: 3641 3641 Size: 6316 MfeI C'AATT_G Cuts at: 3915 3915 Size: 6316 MluI A'CGCG_T Cuts at: 6192 6192 Size: 6316 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5430 5614 1305 Size: 4125 184 2007 Fragments arranged by size: 4125 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3076 10 Size: 54 1425 343 1244 3250 Fragments arranged by size: 3250 1425 1244 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2534 2915 3182 4274 4633 4792 518 Size: 648 1368 381 267 1092 359 159 2042 Fragments arranged by size: 2042 1368 1092 648 381 359 267 159 MspA1I CmG'CkG Cuts at: 1606 2548 3732 4409 5557 5802 1606 Size: 942 1184 677 1148 245 2120 Fragments arranged by size: 2120 1184 1148 942 677 245 NaeI GCC'GGC Cuts at: 3311 3311 Size: 6316 NcoI C'CATG_G Cuts at: 513 1065 2004 2667 513 Size: 552 939 663 4162 Fragments arranged by size: 4162 939 663 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6316 NgoMIV G'CCGG_C Cuts at: 3309 3309 Size: 6316 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6316 NotI GC'GGCC_GC Cuts at: 3763 3763 Size: 6316 NspI r_CATG'y Cuts at: 1132 1229 3674 5219 1132 Size: 97 2445 1545 2229 Fragments arranged by size: 2445 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3067 1348 Size: 1719 4597 PciI A'CATG_T Cuts at: 5215 5215 Size: 6316 PflMI CCAn_nnn'nTGG Cuts at: 1108 3517 1108 Size: 2409 3907 PfoI T'CCnGG_A Cuts at: 2163 3494 2163 Size: 1331 4985 PmeI GTTT'AAAC Cuts at: 3620 3620 Size: 6316 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4385 4417 5898 5930 4385 Size: 32 1481 32 4771 Fragments arranged by size: 4771 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2760 2760 Size: 6316 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6316 PsiI TTA'TAA Cuts at: 3886 3886 Size: 6316 PstI C_TGCA'G Cuts at: 830 2208 2274 3555 3668 830 Size: 1378 66 1281 113 3478 Fragments arranged by size: 3478 1378 1281 113 66 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6316 PvuII CAG'CTG Cuts at: 2548 2548 Size: 6316 SalI G'TCGA_C Cuts at: 1936 3658 1936 Size: 1722 4594 SanDI GG'GwC_CC Cuts at: 2760 2760 Size: 6316 SapI GCTCTTCn'nnn_ Cuts at: 2693 2693 Size: 6316 SbfI CC_TGCA'GG Cuts at: 2274 3555 3668 2274 Size: 1281 113 4922 Fragments arranged by size: 4922 1281 113 ScaI AGT'ACT Cuts at: 3326 4564 6276 3326 Size: 1238 1712 3366 Fragments arranged by size: 3366 1712 1238 SfcI C'TryA_G Cuts at: 826 1045 2204 2270 3551 3664 4799 5480 5671 Size: 219 1159 66 1281 113 1135 681 191 Cuts at: 5671 826 Size: 1471 Fragments arranged by size: 1471 1281 1159 1135 681 219 191 113 66 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6316 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6316 SmaI CCC'GGG Cuts at: 1872 2015 2228 3533 3643 1872 Size: 143 213 1305 110 4545 Fragments arranged by size: 4545 1305 213 143 110 SmlI C'TyrA_G Cuts at: 820 1017 1943 2357 2855 4420 5321 5583 820 Size: 197 926 414 498 1565 901 262 1553 Fragments arranged by size: 1565 1553 926 901 498 414 262 197 SnaBI TAC'GTA Cuts at: 493 4107 493 Size: 3614 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6316 SphI G_CATG'C Cuts at: 1132 3674 1132 Size: 2542 3774 SspI AAT'ATT Cuts at: 5 52 4240 5 Size: 47 4188 2081 Fragments arranged by size: 4188 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6316 StyI C'CwwG_G Cuts at: 513 1065 2004 2667 3755 513 Size: 552 939 663 1088 3074 Fragments arranged by size: 3074 1088 939 663 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2647 4343 4360 4513 4698 5143 325 Size: 1255 1067 1696 17 153 185 445 1498 Fragments arranged by size: 1696 1498 1255 1067 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2344 3249 3324 Size: 274 80 33 51 1443 367 905 75 Cuts at: 3324 3357 4562 6274 96 Size: 33 1205 1712 138 Fragments arranged by size: 1712 1443 1205 905 367 274 138 80 75 51 33 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2622 2626 2654 2658 3548 3580 6276 6308 2622 Size: 4 28 4 890 32 2696 32 2630 Fragments arranged by size: 2696 2630 890 32 32 28 4 4 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6316 XbaI T'CTAG_A Cuts at: 3652 3652 Size: 6316 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2908 3295 2908 Size: 387 5929 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6316 XmaI C'CCGG_G Cuts at: 1870 2013 2226 3531 3641 1870 Size: 143 213 1305 110 4545 Fragments arranged by size: 4545 1305 213 143 110 XmnI GAAnn'nnTTC Cuts at: 4445 4445 Size: 6316 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4124 276 Size: 53 83 186 936 2590 2468 Fragments arranged by size: 2590 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I EcoICRI EcoRV FalI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SexAI SfiI SfoI SrfI SwaI