Restriction map for
pFN21AB8299
(Linear) (Six-base) MAPSORT of: KIBB8299.seq Check: 1480 from: 1 to: 1611
KIBB8299.orf
With 182 enzymes: SgfI *
March 3, 2008 11:04 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 632 1000 1075 1611
Size: 632 368 75 536
Fragments arranged by size:
632 536 368 75
AleI CACnn'nnGTG
Cuts at: 0 529 1177 1611
Size: 529 648 434
Fragments arranged by size:
648 529 434
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 294 388 547 1611
Size: 294 94 159 1064
Fragments arranged by size:
1064 294 159 94
AlwNI CAG_nnn'CTG
Cuts at: 0 264 399 543 555 1086 1611
Size: 264 135 144 12 531 525
Fragments arranged by size:
531 525 264 144 135 12
AvaI C'yCGr_G
Cuts at: 0 8 221 1309 1526 1611
Size: 8 213 1088 217 85
Fragments arranged by size:
1088 217 213 85 8
BanII G_rGCy'C
Cuts at: 0 8 1611
Size: 8 1603
BbsI GAAGACnn'nnnn_
Cuts at: 0 734 1611
Size: 734 877
BlpI GC'TnA_GC
Cuts at: 0 376 1003 1611
Size: 376 627 608
Fragments arranged by size:
627 608 376
Bme1580I G_kGCm'C
Cuts at: 0 936 1026 1232 1611
Size: 936 90 206 379
Fragments arranged by size:
936 379 206 90
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1010 1611
Size: 1010 601
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 607 812 898 1473 1611
Size: 364 243 205 86 575 138
Fragments arranged by size:
575 364 243 205 138 86
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 835 1611
Size: 337 498 776
Fragments arranged by size:
776 498 337
BsaI GGTCTCn'nnnn_
Cuts at: 0 156 788 1611
Size: 156 632 823
Fragments arranged by size:
823 632 156
BsaWI w'CCGG_w
Cuts at: 0 125 1611
Size: 125 1486
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 617 647 1471 1501 1611
Size: 617 30 824 30 110
Fragments arranged by size:
824 617 110 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 55 474 490 820 859 928 1414 1450
Size: 55 419 16 330 39 69 486 36
Cuts at: 1450 1575 1611
Size: 125 36
Fragments arranged by size:
486 419 330 125 69 55 39 36
36 16
BseYI C'CCAG_C
Cuts at: 0 597 1038 1611
Size: 597 441 573
Fragments arranged by size:
597 573 441
BsiHKAI G_wGCw'C
Cuts at: 0 86 377 683 1219 1451 1611
Size: 86 291 306 536 232 160
Fragments arranged by size:
536 306 291 232 160 86
BsmI GAATG_Cn'
Cuts at: 0 1266 1611
Size: 1266 345
BsmBI CGTCTCn'nnnn_
Cuts at: 0 25 1611
Size: 25 1586
Bsp1286I G_dGCh'C
Cuts at: 0 8 86 377 683 936 1026 1219 1232
Size: 8 78 291 306 253 90 193 13
Cuts at: 1232 1451 1611
Size: 219 160
Fragments arranged by size:
306 291 253 219 193 160 90 78
13 8
BspHI T'CATG_A
Cuts at: 0 1157 1611
Size: 1157 454
BsrBI CCG'CTC
Cuts at: 0 657 1611
Size: 657 954
BsrFI r'CCGG_y
Cuts at: 0 1304 1611
Size: 1304 307
BssSI C'ACGA_G
Cuts at: 0 370 1450 1611
Size: 370 1080 161
Fragments arranged by size:
1080 370 161
BstEII G'GTnAC_C
Cuts at: 0 1467 1611
Size: 1467 144
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1464 1611
Size: 1464 147
BstYI r'GATC_y
Cuts at: 0 668 1611
Size: 668 943
Bsu36I CC'TnA_GG
Cuts at: 0 1118 1611
Size: 1118 493
BtgI C'CryG_G
Cuts at: 0 26 662 813 896 1611
Size: 26 636 151 83 715
Fragments arranged by size:
715 636 151 83 26
BtsI GCAGTG_nn'
Cuts at: 0 830 1611
Size: 830 781
DraIII CAC_nnn'GTG
Cuts at: 0 774 825 1611
Size: 774 51 786
Fragments arranged by size:
786 774 51
DrdI GACnn_nn'nnGTC
Cuts at: 0 295 1611
Size: 295 1316
EaeI y'GGCC_r
Cuts at: 0 816 1069 1611
Size: 816 253 542
Fragments arranged by size:
816 542 253
EarI CTCTTCn'nnn_
Cuts at: 0 124 508 688 1177 1611
Size: 124 384 180 489 434
Fragments arranged by size:
489 434 384 180 124
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 154 1141 1611
Size: 154 987 470
Fragments arranged by size:
987 470 154
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 364 607 632 812 898 1000 1075 1473
Size: 364 243 25 180 86 102 75 398
Cuts at: 1473 1611
Size: 138
Fragments arranged by size:
398 364 243 180 138 102 86 75
25
EcoNI CCTnn'n_nnAGG
Cuts at: 0 163 1611
Size: 163 1448
EcoO109I rG'GnC_Cy
Cuts at: 0 755 1287 1611
Size: 755 532 324
Fragments arranged by size:
755 532 324
FseI GG_CCGG'CC
Cuts at: 0 1308 1611
Size: 1308 303
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1471 1503 1582 1611
Size: 1471 32 79 29
Fragments arranged by size:
1471 79 32 29
HindIII A'AGCT_T
Cuts at: 0 1144 1611
Size: 1144 467
MscI TGG'CCA
Cuts at: 0 1071 1611
Size: 1071 540
MslI CAynn'nnrTG
Cuts at: 0 529 910 1177 1611
Size: 529 381 267 434
Fragments arranged by size:
529 434 381 267
MspA1I CmG'CkG
Cuts at: 0 543 1611
Size: 543 1068
NaeI GCC'GGC
Cuts at: 0 1306 1611
Size: 1306 305
NcoI C'CATG_G
Cuts at: 0 662 1611
Size: 662 949
NgoMIV G'CCGG_C
Cuts at: 0 1304 1611
Size: 1304 307
PasI CC'CwG_GG
Cuts at: 0 1062 1611
Size: 1062 549
PflMI CCAn_nnn'nTGG
Cuts at: 0 1512 1611
Size: 1512 99
PfoI T'CCnGG_A
Cuts at: 0 158 1489 1611
Size: 158 1331 122
Fragments arranged by size:
1331 158 122
PpuMI rG'GwC_Cy
Cuts at: 0 755 1611
Size: 755 856
PstI C_TGCA'G
Cuts at: 0 203 269 1550 1611
Size: 203 66 1281 61
Fragments arranged by size:
1281 203 66 61
PvuII CAG'CTG
Cuts at: 0 543 1611
Size: 543 1068
SanDI GG'GwC_CC
Cuts at: 0 755 1611
Size: 755 856
SapI GCTCTTCn'nnn_
Cuts at: 0 688 1611
Size: 688 923
SbfI CC_TGCA'GG
Cuts at: 0 269 1550 1611
Size: 269 1281 61
Fragments arranged by size:
1281 269 61
ScaI AGT'ACT
Cuts at: 0 1321 1611
Size: 1321 290
SfcI C'TryA_G
Cuts at: 0 199 265 1546 1611
Size: 199 66 1281 65
Fragments arranged by size:
1281 199 66 65
SmaI CCC'GGG
Cuts at: 0 10 223 1528 1611
Size: 10 213 1305 83
Fragments arranged by size:
1305 213 83 10
SmlI C'TyrA_G
Cuts at: 0 352 850 1611
Size: 352 498 761
Fragments arranged by size:
761 498 352
StyI C'CwwG_G
Cuts at: 0 662 1611
Size: 662 949
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 642 1611
Size: 642 969
TatI w'GTAC_w
Cuts at: 0 339 1244 1319 1352 1611
Size: 339 905 75 33 259
Fragments arranged by size:
905 339 259 75 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 617 621 649 653 1543 1575 1611
Size: 617 4 28 4 890 32 36
Fragments arranged by size:
890 617 36 32 28 4 4
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 903 1290 1611
Size: 903 387 321
Fragments arranged by size:
903 387 321
XmaI C'CCGG_G
Cuts at: 0 8 221 1526 1611
Size: 8 213 1305 85
Fragments arranged by size:
1305 213 85 8
Enzymes that do cut:
AcuI AleI AlfI AlwNI AvaI BanII BbsI BlpI
Bme1580I BplI BpmI BpuEI BsaI BsaWI BsaXI BseRI
BseYI BsiHKAI BsmI BsmBI Bsp1286I BspHI BsrBI BsrFI
BssSI BstEII BstXI BstYI Bsu36I BtgI BtsI DraIII
DrdI EaeI EarI EciI Eco57MI EcoNI EcoO109I FseI
Hin4I HindIII MscI MslI MspA1I NaeI NcoI NgoMIV
PasI PflMI PfoI PpuMI PstI PvuII SanDI SapI
SbfI ScaI SfcI SmaI SmlI StyI TaqII TatI
TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BmgBI BmrI BmtI
Bpu10I BsaAI BsaBI BsaHI BsgI BsiEI BsiWI BspEI
BspMI BsrDI BsrGI BssHII BstAPI BstBI BstZ17I BtgZI
ClaI CspCI DraI EagI EcoICRI EcoRI EcoRV FalI
FspI FspAI HaeII HincII HpaI KasI KpnI MfeI
MluI MmeI NarI NdeI NheI NotI NruI NsiI
NspI PacI PciI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI SwaI Tth111I XbaI XhoI XmnI
ZraI