Restriction map for
pFN21AB8295
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# NO CUT ENZYMES ( 114 enzymes) #
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AatII AccI AccII AccIII AclI
AcsI AflII ApoI AscI AseI
AsnI AspI AviII BalI BbrPI
BbsI BciVI BclI BfrI BglI
BlpI Bpu1102I BpuAI BsaBI BseAI
BsiEI BsiWI BsmFI BspDI BspEI
BspHI BspLU11I BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
CelII ClaI CpoI DraI DrdI
DsaI EaeI EagI EclXI Eco52I
EcoO65I EcoRI EcoT22I FbaI FseI
FspI HgaI HincII HindII HpaI
KspI MamI MfeI MluI MluNI
MroI MscI MunI MvnI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacII
SalI SbfI ScaI SfaNI SfiI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspI SwaI Tth111I Van91I
XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Acc65I 155 GGTACC G^GTACC
AcyI 1071 GGCGCC G[GA]^CG[CT]C
AflIII 914 ACGTGT A^C[GA][CT]GT
AgeI 426 ACCGGT A^CCGGT
AhdI 392 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 918 GTGCAC G^TGCAC
ApaLI 918 GTGCAC G^TGCAC
Asp718I 155 GGTACC G^GTACC
AspEI 392 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 40 CCTAGG C^CTAGG
BamHI 1139 GGATCC G^GATCC
BbeI 1071 GGCGCC GGCGC^C
BcgI 473 CGAGCAGCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 363 AGATCT A^GATCT
BlnI 40 CCTAGG C^CTAGG
BsaAI 913 TACGTG [CT]AC^GT[GA]
BsaHI 1071 GGCGCC G[GA]^CG[CT]C
BsgI 313 GTGCAG GTGCAG(16/14)
BsmBI 822 GAGACG CGTCTC(1/5)
BsmI 1055 GAATGC GAATGC(1/-1)
Bsp1407I 533 TGTACA T^GTACA
BspMI 57 GCAGGT ACCTGC(4/8)
BsrBI 370 GAGCGG CCGCTC(-3/-3)
BsrDI 322 CATTGC GCAATG(2/0)
BsrGI 533 TGTACA T^GTACA
BssSI 613 CACGAG CACGAG(-5/-1)
BstXI 540 CCAAGAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Eam1105I 392 GACACCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 441 GATATC GAT^ATC
Hin1I 1071 GGCGCC G[GA]^CG[CT]C
KasI 1071 GGCGCC G^GCGCC
KpnI 155 GGTACC GGTAC^C
MseI 1043 TTAA T^TAA
MspA1I 1107 CAGCGG C[AC]G^C[GT]G
NarI 1071 GGCGCC GG^CGCC
PinAI 426 ACCGGT A^CCGGT
SapI 1182 GAAGAGC GCTCTTC(1/4)
SexAI 395 ACCAGGT A^CC[AT]GGT
SfoI 1071 GGCGCC GGC^GCC
SmlI 569 CTCAAG C^T[CT][GA]AG
SspBI 533 TGTACA T^GTACA
Tru9I 1043 TTAA T^TAA
Tsp509I 672 AATT ^AATT
XcmI 159 CCATGATACCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AocI 449 CCTCAGG CC^T[ACGT]AGG
697 CCTGAGG
Aor51HI 137 AGCGCT AGC^GCT
334 AGCGCT
ApaI 36 GGGCCC GGGCC^C
526 GGGCCC
BanI 155 GGTACC G^G[CT][GA]CC
1071 GGCGCC
BbvCI 1206 CCTCAGC CCTCAGC(-5/-2)
219 GCTGAGG
BsaI 499 GAGACC GGTCTC(1/5)
399 GGTCTC
BsaWI 426 ACCGGT [AT]^CCGG[AT]
1231 ACCGGA
BsrFI 426 ACCGGT [GA]^CCGG[CT]
989 ACCGGC
Bsu36I 449 CCTCAGG CC^T[ACGT]AGG
697 CCTGAGG
Cfr10I 426 ACCGGT [GA]^CCGG[CT]
989 ACCGGC
DraIII 921 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
979 CACTTGGTG
Eco47III 137 AGCGCT AGC^GCT
334 AGCGCT
Eco81I 449 CCTCAGG CC^T[ACGT]AGG
697 CCTGAGG
EcoNI 288 CCTGCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
808 CCTACCCCAGG
HindIII 935 AAGCTT A^AGCTT
1016 AAGCTT
MaeII 825 ACGT A^CGT
914 ACGT
NspI 479 GCATGC [GA]CATG^[CT]
1197 GCATGC
SacI 298 GAGCTC GAGCT^C
1029 GAGCTC
SfcI 969 CTACAG C^T[GA][CT]AG
1359 CTGTAG
SphI 479 GCATGC GCATG^C
1197 GCATGC
StuI 43 AGGCCT AGG^CCT
305 AGGCCT
TaqI 83 TCGA T^CGA
359 TCGA
TthHB8I 83 TCGA T^CGA
359 TCGA
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# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
Asp700I AspHI AvaI AvaII BanII
BbvI BcnI BfaI BmyI BpmI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MslI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
StyI TfiI TseI Tsp45I TspRI
XhoII XmnI