(Circular) (Six-base) MAPSORT of: pFN21AB8295.seq Check: 5955 from: 1 to: 6124 pFN21AB8295 6124 bp With 182 enzymes: SgfI * February 27, 2009 14:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2053 2053 Size: 6124 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3934 278 Size: 53 83 186 936 2398 2468 Fragments arranged by size: 2468 2398 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3467 1937 Size: 1530 4594 Acc65I G'GTAC_C Cuts at: 2160 3445 2160 Size: 1285 4839 AclI AA'CG_TT Cuts at: 1563 4251 4624 1563 Size: 2688 373 3063 Fragments arranged by size: 3063 2688 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2131 2364 2670 3178 4187 5571 5839 1632 Size: 499 233 306 508 1009 1384 268 1917 Fragments arranged by size: 1917 1384 1009 508 499 306 268 233 AfeI AGC'GCT Cuts at: 2144 2341 2144 Size: 197 5927 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5927 AflIII A'CryG_T Cuts at: 1203 2919 5023 6000 1203 Size: 1716 2104 977 1327 Fragments arranged by size: 2104 1716 1327 977 AgeI A'CCGG_T Cuts at: 2431 3421 4955 2431 Size: 990 1534 3600 Fragments arranged by size: 3600 1534 990 AhdI GACnn_n'nnGTC Cuts at: 2402 4853 2402 Size: 2451 3673 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6124 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6124 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2552 2584 2552 Size: 32 6092 AlwNI CAG_nnn'CTG Cuts at: 2257 2284 2462 3867 5439 2257 Size: 27 178 1405 1572 2942 Fragments arranged by size: 2942 1572 1405 178 27 ApaI G_GGCC'C Cuts at: 2045 2535 2045 Size: 490 5634 ApaLI G'TGCA_C Cuts at: 2923 4181 5337 2923 Size: 1258 1156 3710 Fragments arranged by size: 3710 1258 1156 ApoI r'AATT_y Cuts at: 1493 3433 3659 3811 5920 1493 Size: 1940 226 152 2109 1697 Fragments arranged by size: 2109 1940 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4678 160 Size: 872 3646 1606 Fragments arranged by size: 3646 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6124 AvaI C'yCGr_G Cuts at: 1870 1943 2181 2231 2476 3449 1870 Size: 73 238 50 245 973 4545 Fragments arranged by size: 4545 973 245 238 73 50 AvrII C'CTAG_G Cuts at: 2045 2045 Size: 6124 BamHI G'GATC_C Cuts at: 3144 3454 3821 3144 Size: 310 367 5447 Fragments arranged by size: 5447 367 310 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2160 3076 3445 4900 618 Size: 325 217 627 373 916 369 1455 1842 Fragments arranged by size: 1842 1455 916 627 373 369 325 217 BanII G_rGCy'C Cuts at: 1391 2045 2307 2535 2664 3038 3443 1391 Size: 654 262 228 129 374 405 4072 Fragments arranged by size: 4072 654 405 374 262 228 129 BbeI G_GCGC'C Cuts at: 3080 3080 Size: 6124 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5153 BbvCI CC'TCA_GC Cuts at: 2225 3212 2225 Size: 987 5137 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2467 2501 4315 4349 1903 Size: 34 530 34 1814 34 3678 Fragments arranged by size: 3678 1814 530 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4016 5226 4016 Size: 1210 4914 BclI T'GATC_A Cuts at: 1548 1548 Size: 6124 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4735 136 Size: 107 122 71 631 605 3063 1525 Fragments arranged by size: 3063 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2368 6119 2368 Size: 3751 2373 BlpI GC'TnA_GC Cuts at: 3541 3541 Size: 6124 Bme1580I G_kGCm'C Cuts at: 1790 2045 2535 2927 4185 5341 1790 Size: 255 490 392 1258 1156 2573 Fragments arranged by size: 2573 1258 1156 490 392 255 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6124 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2027 2913 3162 Size: 416 53 83 399 24 610 886 249 Cuts at: 3162 3855 4813 442 Size: 693 958 1753 Fragments arranged by size: 1753 958 886 693 610 416 399 249 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6124 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2794 2794 Size: 6124 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2147 2279 2291 2294 2393 3426 4784 1239 Size: 908 132 12 3 99 1033 1358 2579 Fragments arranged by size: 2579 1358 1033 908 132 99 12 3 Bpu10I CC'TnA_GC Cuts at: 2192 2225 3212 2192 Size: 33 987 5104 Fragments arranged by size: 5104 987 33 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2559 4249 5114 5412 2559 Size: 1690 865 298 3271 Fragments arranged by size: 3271 1690 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2410 2498 4787 882 Size: 617 911 88 2289 2219 Fragments arranged by size: 2289 2219 911 617 88 BsaAI yAC'GTr Cuts at: 493 1204 2920 3915 493 Size: 711 1716 995 2702 Fragments arranged by size: 2702 1716 995 711 BsaBI GATnn'nnATC Cuts at: 6118 6118 Size: 6124 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3077 3931 4313 4948 Size: 53 83 186 936 1544 854 382 635 Cuts at: 4948 275 Size: 1451 Fragments arranged by size: 1544 1451 936 854 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2431 3236 3421 4557 4955 5229 5376 5787 2431 Size: 805 185 1136 398 274 147 411 2768 Fragments arranged by size: 2768 1136 805 411 398 274 185 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2552 2582 3272 3302 1178 Size: 30 165 30 1149 30 690 30 4000 Fragments arranged by size: 4000 1149 690 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2105 2351 2403 2443 2573 1186 Size: 919 246 52 40 130 4737 Fragments arranged by size: 4737 919 246 130 52 40 BseYI C'CCAG_C Cuts at: 2613 5327 5823 2613 Size: 2714 496 2914 Fragments arranged by size: 2914 2714 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2339 1163 Size: 573 603 4948 Fragments arranged by size: 4948 603 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3574 4335 5363 1540 Size: 275 186 1573 761 1028 2301 Fragments arranged by size: 2301 1573 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2211 2307 2927 3038 4185 4270 5341 2211 Size: 96 620 111 1147 85 1071 2994 Fragments arranged by size: 2994 1147 1071 620 111 96 85 BsmI GAATG_Cn' Cuts at: 3066 3635 3728 3066 Size: 569 93 5462 Fragments arranged by size: 5462 569 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2821 2821 Size: 6124 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2045 2211 2307 2535 2664 2927 3038 Size: 399 255 166 96 228 129 263 111 Cuts at: 3038 3443 4185 4270 5341 1391 Size: 405 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 405 399 263 255 228 166 129 111 96 85 BspEI T'CCGG_A Cuts at: 5787 5787 Size: 6124 BspHI T'CATG_A Cuts at: 4013 5743 4013 Size: 1730 4394 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2053 3479 844 Size: 1209 1426 3489 Fragments arranged by size: 3489 1426 1209 BsrBI CCG'CTC Cuts at: 1394 1823 2377 4011 1394 Size: 429 554 1634 3507 Fragments arranged by size: 3507 1634 554 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2326 4619 4793 65 Size: 1183 1078 2293 174 1396 Fragments arranged by size: 2293 1396 1183 1078 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2431 2994 3421 4768 4955 1700 Size: 110 621 563 427 1347 187 2869 Fragments arranged by size: 2869 1347 621 563 427 187 110 BsrGI T'GTAC_A Cuts at: 96 2538 96 Size: 2442 3682 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6124 BssSI C'ACGA_G Cuts at: 2618 4184 5196 2618 Size: 1566 1012 3546 Fragments arranged by size: 3546 1566 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6124 BstBI TT'CG_AA Cuts at: 4928 4928 Size: 6124 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6124 BstXI CCAn_nnnn'nTGG Cuts at: 2552 2552 Size: 6124 BstYI r'GATC_y Cuts at: 1972 2235 2253 2368 2578 3144 3454 3821 4206 Size: 263 18 115 210 566 310 367 385 Cuts at: 4206 4223 5675 5761 5773 6119 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 566 385 367 346 310 263 210 115 86 18 17 12 Bsu36I CC'TnA_GG Cuts at: 2455 2703 2455 Size: 248 5876 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5572 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5385 Fragments arranged by size: 5385 683 56 BtsI GCAGTG_nn' Cuts at: 2729 3640 4434 4454 2729 Size: 911 794 20 4399 Fragments arranged by size: 4399 911 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6124 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2481 2516 3079 3114 529 Size: 35 1917 35 563 35 3539 Fragments arranged by size: 3539 1917 563 35 35 35 DraI TTT'AAA Cuts at: 3428 3428 Size: 6124 DraIII CAC_nnn'GTG Cuts at: 2931 2989 2931 Size: 58 6066 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5131 809 Size: 507 3815 1802 Fragments arranged by size: 3815 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3571 4460 6089 Size: 54 1425 50 275 18 1741 889 1629 Cuts at: 6089 8 Size: 43 Fragments arranged by size: 1741 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3571 1537 Size: 275 1759 4090 Fragments arranged by size: 4090 1759 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2094 2340 2769 3066 3182 4054 1353 Size: 741 246 429 297 116 872 3423 Fragments arranged by size: 3423 872 741 429 297 246 116 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2126 4711 5085 5231 387 Size: 1064 138 537 2585 374 146 1280 Fragments arranged by size: 2585 1280 1064 537 374 146 138 EcoICRI GAG'CTC Cuts at: 2305 3036 2305 Size: 731 5393 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2131 2147 2279 2291 2294 2364 2393 Size: 393 499 16 132 12 3 70 29 Cuts at: 2393 2670 3178 3426 4187 4784 5571 5839 1239 Size: 277 508 248 761 597 787 268 1524 Fragments arranged by size: 1524 787 761 597 508 499 393 277 268 248 132 70 29 16 12 3 EcoNI CCTnn'n_nnAGG Cuts at: 2297 2817 2297 Size: 520 5604 EcoO109I rG'GnC_Cy Cuts at: 2042 2069 2188 2531 2532 2042 Size: 27 119 343 1 5634 Fragments arranged by size: 5634 343 119 27 1 EcoRI G'AATT_C Cuts at: 3433 3811 3433 Size: 378 5746 EcoRV GAT'ATC Cuts at: 2448 2448 Size: 6124 FspI TGC'GCA Cuts at: 4630 4630 Size: 6124 HaeII r_GCGC'y Cuts at: 1678 2146 2343 3080 5271 1678 Size: 468 197 737 2191 2531 Fragments arranged by size: 2531 2191 737 468 197 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2388 2420 2432 2464 3181 Size: 32 135 32 816 32 12 32 717 Cuts at: 3181 3213 4765 4797 4839 4871 1373 Size: 32 1552 32 42 32 2626 Fragments arranged by size: 2626 1552 816 717 135 42 32 32 32 32 32 32 32 12 HincII GTy'rAC Cuts at: 669 1657 1938 3468 3714 669 Size: 988 281 1530 246 3079 Fragments arranged by size: 3079 1530 988 281 246 HindIII A'AGCT_T Cuts at: 748 2940 3021 748 Size: 2192 81 3851 Fragments arranged by size: 3851 2192 81 HpaI GTT'AAC Cuts at: 3714 3714 Size: 6124 KasI G'GCGC_C Cuts at: 3076 3076 Size: 6124 KpnI G_GTAC'C Cuts at: 2164 3449 2164 Size: 1285 4839 MfeI C'AATT_G Cuts at: 3723 3723 Size: 6124 MluI A'CGCG_T Cuts at: 6000 6000 Size: 6124 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3315 5238 5422 1305 Size: 2010 1923 184 2007 Fragments arranged by size: 2010 2007 1923 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4302 Fragments arranged by size: 4302 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3085 3253 3348 4082 4441 4600 518 Size: 648 1919 168 95 734 359 159 2042 Fragments arranged by size: 2042 1919 734 648 359 168 159 95 MspA1I CmG'CkG Cuts at: 1606 3114 3540 4217 5365 5610 1606 Size: 1508 426 677 1148 245 2120 Fragments arranged by size: 2120 1508 1148 677 426 245 NarI GG'CG_CC Cuts at: 3077 3077 Size: 6124 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5572 NdeI CA'TA_TG Cuts at: 387 387 Size: 6124 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6124 NotI GC'GGCC_GC Cuts at: 3571 3571 Size: 6124 NspI r_CATG'y Cuts at: 1132 1229 2488 3206 3482 5027 1132 Size: 97 1259 718 276 1545 2229 Fragments arranged by size: 2229 1545 1259 718 276 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6124 PciI A'CATG_T Cuts at: 5023 5023 Size: 6124 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6124 PfoI T'CCnGG_A Cuts at: 2256 2256 Size: 6124 PmeI GTTT'AAAC Cuts at: 3428 3428 Size: 6124 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2552 2584 2784 2816 4193 4225 5706 5738 2552 Size: 32 200 32 1377 32 1481 32 2938 Fragments arranged by size: 2938 1481 1377 200 32 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6124 PsiI TTA'TAA Cuts at: 3694 3694 Size: 6124 PspOMI G'GGCC_C Cuts at: 2041 2531 2041 Size: 490 5634 PstI C_TGCA'G Cuts at: 830 3476 830 Size: 2646 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6124 SacI G_AGCT'C Cuts at: 2307 3038 2307 Size: 731 5393 SalI G'TCGA_C Cuts at: 1936 3466 1936 Size: 1530 4594 SapI GCTCTTCn'nnn_ Cuts at: 3182 3182 Size: 6124 SbfI CC_TGCA'GG Cuts at: 3476 3476 Size: 6124 ScaI AGT'ACT Cuts at: 4372 6084 4372 Size: 1712 4412 SexAI A'CCwGG_T Cuts at: 2400 2400 Size: 6124 SfcI C'TryA_G Cuts at: 826 1045 2974 3364 3472 4607 5288 5479 826 Size: 219 1929 390 108 1135 681 191 1471 Fragments arranged by size: 1929 1471 1135 681 390 219 191 108 SfoI GGC'GCC Cuts at: 3078 3078 Size: 6124 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6124 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6124 SmaI CCC'GGG Cuts at: 1872 3451 1872 Size: 1579 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2574 4228 5129 5391 820 Size: 197 926 631 1654 901 262 1553 Fragments arranged by size: 1654 1553 926 901 631 262 197 SnaBI TAC'GTA Cuts at: 493 3915 493 Size: 3422 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6124 SphI G_CATG'C Cuts at: 1132 2488 3206 3482 1132 Size: 1356 718 276 3774 Fragments arranged by size: 3774 1356 718 276 SspI AAT'ATT Cuts at: 5 52 4048 5 Size: 47 3996 2081 Fragments arranged by size: 3996 2081 47 StuI AGG'CCT Cuts at: 1517 2050 2312 1517 Size: 533 262 5329 Fragments arranged by size: 5329 533 262 StyI C'CwwG_G Cuts at: 513 1065 2013 2045 2244 2499 3563 513 Size: 552 948 32 199 255 1064 3074 Fragments arranged by size: 3074 1064 948 552 255 199 32 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2305 4151 4168 4321 4506 4951 325 Size: 1255 725 1846 17 153 185 445 1498 Fragments arranged by size: 1846 1498 1255 725 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2538 4370 6082 Size: 274 80 33 51 1443 561 1832 1712 Cuts at: 6082 96 Size: 138 Fragments arranged by size: 1832 1712 1443 561 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6084 6116 6084 Size: 32 6092 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6124 XbaI T'CTAG_A Cuts at: 3460 3460 Size: 6124 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2171 2171 Size: 6124 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6124 XmaI C'CCGG_G Cuts at: 1870 3449 1870 Size: 1579 4545 XmnI GAAnn'nnTTC Cuts at: 2799 2942 3191 3275 4253 2799 Size: 143 249 84 978 4670 Fragments arranged by size: 4670 978 249 143 84 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3932 276 Size: 53 83 186 936 2398 2468 Fragments arranged by size: 2468 2398 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SacI SalI SapI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AscI BaeI BfrBI BsiWI BstZ17I FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI PvuII RsrII SacII SanDI SfiI SrfI SwaI