(Circular) (Six-base) MAPSORT of: pFN21AB8294.seq Check: 2104 from: 1 to: 6778 pFN21AB8294 6778 bp With 182 enzymes: SgfI * January 31, 2011 17:48 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4588 278 Size: 53 83 186 936 3052 2468 Fragments arranged by size: 3052 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4121 1937 Size: 2184 4594 Acc65I G'GTAC_C Cuts at: 3917 4099 3917 Size: 182 6596 AclI AA'CG_TT Cuts at: 1563 4905 5278 1563 Size: 3342 373 3063 Fragments arranged by size: 3342 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2522 2924 3189 3305 3822 4841 6225 6493 Size: 890 402 265 116 517 1019 1384 268 Cuts at: 6493 1632 Size: 1917 Fragments arranged by size: 1917 1384 1019 890 517 402 268 265 116 AfeI AGC'GCT Cuts at: 3989 3989 Size: 6778 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6581 AflIII A'CryG_T Cuts at: 1203 5677 6654 1203 Size: 4474 977 1327 Fragments arranged by size: 4474 1327 977 AgeI A'CCGG_T Cuts at: 5609 5609 Size: 6778 AhdI GACnn_n'nnGTC Cuts at: 5507 5507 Size: 6778 AleI CACnn'nnGTG Cuts at: 1166 2090 2195 2247 1166 Size: 924 105 52 5697 Fragments arranged by size: 5697 924 105 52 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6778 AlwNI CAG_nnn'CTG Cuts at: 2142 2953 4521 6093 2142 Size: 811 1568 1572 2827 Fragments arranged by size: 2827 1572 1568 811 ApaI G_GGCC'C Cuts at: 3049 3947 3049 Size: 898 5880 ApaLI G'TGCA_C Cuts at: 4835 5991 4835 Size: 1156 5622 ApoI r'AATT_y Cuts at: 1493 2893 4087 4313 4465 6574 1493 Size: 1400 1194 226 152 2109 1697 Fragments arranged by size: 2109 1697 1400 1194 226 152 AseI AT'TA_AT Cuts at: 160 1032 5332 160 Size: 872 4300 1606 Fragments arranged by size: 4300 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6778 AvaI C'yCGr_G Cuts at: 1870 1943 2555 2790 3369 3705 4103 1870 Size: 73 612 235 579 336 398 4545 Fragments arranged by size: 4545 612 579 398 336 235 73 AvrII C'CTAG_G Cuts at: 2986 2986 Size: 6778 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3383 3416 3383 Size: 33 6745 BamHI G'GATC_C Cuts at: 4108 4475 4108 Size: 367 6411 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2029 2120 3917 4099 5554 Size: 325 217 627 242 91 1797 182 1455 Cuts at: 5554 618 Size: 1842 Fragments arranged by size: 1842 1797 1455 627 325 242 217 182 91 BanII G_rGCy'C Cuts at: 1391 2290 2345 2414 2556 2566 2862 3024 3049 Size: 899 55 69 142 10 296 162 25 Cuts at: 3049 3195 3947 4097 1391 Size: 146 752 150 4072 Fragments arranged by size: 4072 899 752 296 162 150 146 142 69 55 25 10 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2196 3645 928 Size: 971 297 1449 4061 Fragments arranged by size: 4061 1449 971 297 BbvCI CC'TCA_GC Cuts at: 2534 3774 2534 Size: 1240 5538 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4969 5003 1903 Size: 34 3032 34 3678 Fragments arranged by size: 3678 3032 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4670 5880 4670 Size: 1210 5568 BclI T'GATC_A Cuts at: 1548 1548 Size: 6778 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5389 136 Size: 107 122 71 631 605 3717 1525 Fragments arranged by size: 3717 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6773 6773 Size: 6778 BlpI GC'TnA_GC Cuts at: 2289 2856 2946 3189 3593 4195 2289 Size: 567 90 243 404 602 4872 Fragments arranged by size: 4872 602 567 404 243 90 Bme1580I G_kGCm'C Cuts at: 1790 2032 3049 3947 4839 5995 1790 Size: 242 1017 898 892 1156 2573 Fragments arranged by size: 2573 1156 1017 898 892 242 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6778 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2124 3884 3948 Size: 416 53 83 399 24 707 1760 64 Cuts at: 3948 4509 5467 442 Size: 561 958 1753 Fragments arranged by size: 1760 1753 958 707 561 416 399 83 64 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6778 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2309 2309 Size: 6778 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2414 2534 2717 3038 3434 3545 4040 5438 Size: 1175 120 183 321 396 111 495 1398 Cuts at: 5438 1239 Size: 2579 Fragments arranged by size: 2579 1398 1175 495 396 321 183 120 111 Bpu10I CC'TnA_GC Cuts at: 2534 2660 2940 3774 2534 Size: 126 280 834 5538 Fragments arranged by size: 5538 834 280 126 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3049 4903 5768 6066 3049 Size: 1854 865 298 3761 Fragments arranged by size: 3761 1854 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2670 3672 5441 882 Size: 617 1171 1002 1769 2219 Fragments arranged by size: 2219 1769 1171 1002 617 BsaAI yAC'GTr Cuts at: 493 1204 4569 493 Size: 711 3365 2702 Fragments arranged by size: 3365 2702 711 BsaBI GATnn'nnATC Cuts at: 2150 6772 2150 Size: 4622 2156 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4585 4967 5602 275 Size: 53 83 186 936 3052 382 635 1451 Fragments arranged by size: 3052 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5211 5609 5883 6030 6441 5211 Size: 398 274 147 411 5548 Fragments arranged by size: 5548 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2667 2697 3671 3701 1178 Size: 30 165 30 1264 30 974 30 4255 Fragments arranged by size: 4255 1264 974 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2411 2551 2942 3110 3344 3491 3590 3679 Size: 1225 140 391 168 234 147 99 89 Cuts at: 3679 3744 3863 1186 Size: 65 119 4101 Fragments arranged by size: 4101 1225 391 234 168 147 140 119 99 89 65 BseYI C'CCAG_C Cuts at: 2997 3698 5981 6477 2997 Size: 701 2283 496 3298 Fragments arranged by size: 3298 2283 701 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2567 3315 3854 1163 Size: 573 831 748 539 4087 Fragments arranged by size: 4087 831 748 573 539 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4228 4989 6017 1540 Size: 275 186 2227 761 1028 2301 Fragments arranged by size: 2301 2227 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2290 2556 2589 2862 2941 3195 3450 4839 4924 Size: 266 33 273 79 254 255 1389 85 Cuts at: 4924 5995 2290 Size: 1071 3073 Fragments arranged by size: 3073 1389 1071 273 266 255 254 85 79 33 BsmI GAATG_Cn' Cuts at: 4289 4382 4289 Size: 93 6685 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2032 2290 2345 2414 2556 2566 2589 Size: 399 242 258 55 69 142 10 23 Cuts at: 2589 2862 2941 3024 3049 3195 3450 3947 4097 Size: 273 79 83 25 146 255 497 150 Cuts at: 4097 4839 4924 5995 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 497 399 273 258 255 242 150 146 142 85 83 79 69 55 25 23 10 BspEI T'CCGG_A Cuts at: 6441 6441 Size: 6778 BspHI T'CATG_A Cuts at: 4667 6397 4667 Size: 1730 5048 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2280 4133 844 Size: 1436 1853 3489 Fragments arranged by size: 3489 1853 1436 BsrBI CCG'CTC Cuts at: 1394 1823 3238 4665 1394 Size: 429 1415 1427 3507 Fragments arranged by size: 3507 1427 1415 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5273 5447 65 Size: 1183 4025 174 1396 Fragments arranged by size: 4025 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2664 3739 5422 5609 1700 Size: 110 854 1075 1683 187 2869 Fragments arranged by size: 2869 1683 1075 854 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6778 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6778 BssSI C'ACGA_G Cuts at: 4838 5850 4838 Size: 1012 5766 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6778 BstBI TT'CG_AA Cuts at: 5582 5582 Size: 6778 BstEII G'GTnAC_C Cuts at: 1184 2386 1184 Size: 1202 5576 BstXI CCAn_nnnn'nTGG Cuts at: 2333 4034 2333 Size: 1701 5077 BstYI r'GATC_y Cuts at: 1972 3279 4108 4475 4860 4877 6329 6415 6427 Size: 1307 829 367 385 17 1452 86 12 Cuts at: 6427 6773 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1307 829 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2038 2373 2629 513 Size: 552 939 34 335 256 4662 Fragments arranged by size: 4662 939 552 335 256 34 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6039 Fragments arranged by size: 6039 683 56 BtsI GCAGTG_nn' Cuts at: 3886 4294 5088 5108 3886 Size: 408 794 20 5556 Fragments arranged by size: 5556 794 408 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6778 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3070 3105 529 Size: 35 2506 35 4202 Fragments arranged by size: 4202 2506 35 35 DraI TTT'AAA Cuts at: 4082 4082 Size: 6778 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5785 809 Size: 507 4469 1802 Fragments arranged by size: 4469 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3009 3102 3897 Size: 54 1425 50 275 18 1179 93 795 Cuts at: 3897 4225 5114 6743 8 Size: 328 889 1629 43 Fragments arranged by size: 1629 1425 1179 889 795 328 275 93 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4225 1537 Size: 275 2413 4090 Fragments arranged by size: 4090 2413 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2640 2909 4708 1353 Size: 1287 269 1799 3423 Fragments arranged by size: 3423 1799 1287 269 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5365 5739 5885 387 Size: 1064 138 3776 374 146 1280 Fragments arranged by size: 3776 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2288 2554 2860 3193 2288 Size: 266 306 333 5873 Fragments arranged by size: 5873 333 306 266 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2414 2522 2534 2717 2924 3038 3189 Size: 393 782 108 12 183 207 114 151 Cuts at: 3189 3305 3434 3545 3822 4040 4841 5438 6225 Size: 116 129 111 277 218 801 597 787 Cuts at: 6225 6493 1239 Size: 268 1524 Fragments arranged by size: 1524 801 787 782 597 393 277 268 218 207 183 151 129 116 114 111 108 12 EcoNI CCTnn'n_nnAGG Cuts at: 2396 2906 2990 3527 2396 Size: 510 84 537 5647 Fragments arranged by size: 5647 537 510 84 EcoO109I rG'GnC_Cy Cuts at: 2782 3045 3336 3943 3944 4060 2782 Size: 263 291 607 1 116 5500 Fragments arranged by size: 5500 607 291 263 116 1 EcoRI G'AATT_C Cuts at: 4087 4465 4087 Size: 378 6400 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3101 4006 3101 Size: 905 5873 FspI TGC'GCA Cuts at: 5284 5284 Size: 6778 HaeII r_GCGC'y Cuts at: 1678 3991 5925 1678 Size: 2313 1934 2531 Fragments arranged by size: 2531 2313 1934 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2396 2428 3416 3448 3614 Size: 32 135 32 824 32 988 32 166 Cuts at: 3614 3646 3671 3703 3770 3797 3802 3829 5419 Size: 32 25 32 67 27 5 27 1590 Cuts at: 5419 5451 5493 5525 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1590 988 824 166 135 67 42 32 32 32 32 32 32 32 32 27 27 25 5 HincII GTy'rAC Cuts at: 669 1657 1938 2050 3175 4122 4368 669 Size: 988 281 112 1125 947 246 3079 Fragments arranged by size: 3079 1125 988 947 281 246 112 HindIII A'AGCT_T Cuts at: 748 748 Size: 6778 HpaI GTT'AAC Cuts at: 3175 4368 3175 Size: 1193 5585 KpnI G_GTAC'C Cuts at: 3921 4103 3921 Size: 182 6596 MfeI C'AATT_G Cuts at: 4377 4377 Size: 6778 MluI A'CGCG_T Cuts at: 6654 6654 Size: 6778 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5892 6076 1305 Size: 4587 184 2007 Fragments arranged by size: 4587 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3104 3899 10 Size: 54 1425 343 1272 795 2889 Fragments arranged by size: 2889 1425 1272 795 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2090 2195 2197 2247 4032 4736 5095 Size: 648 924 105 2 50 1785 704 359 Cuts at: 5095 5254 518 Size: 159 2042 Fragments arranged by size: 2042 1785 924 704 648 359 159 105 50 2 MspA1I CmG'CkG Cuts at: 1606 2500 2512 3004 3584 3773 3830 4194 4871 Size: 894 12 492 580 189 57 364 677 Cuts at: 4871 6019 6264 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 894 677 580 492 364 245 189 57 12 NaeI GCC'GGC Cuts at: 2666 3741 2666 Size: 1075 5703 NcoI C'CATG_G Cuts at: 513 1065 2004 2373 2629 513 Size: 552 939 369 256 4662 Fragments arranged by size: 4662 939 552 369 256 NdeI CA'TA_TG Cuts at: 387 387 Size: 6778 NgoMIV G'CCGG_C Cuts at: 2664 3739 2664 Size: 1075 5703 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6778 NotI GC'GGCC_GC Cuts at: 4225 4225 Size: 6778 NspI r_CATG'y Cuts at: 1132 1229 4136 5681 1132 Size: 97 2907 1545 2229 Fragments arranged by size: 2907 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6778 PciI A'CATG_T Cuts at: 5677 5677 Size: 6778 PflMI CCAn_nnn'nTGG Cuts at: 1108 2039 2142 3659 1108 Size: 931 103 1517 4227 Fragments arranged by size: 4227 1517 931 103 PfoI T'CCnGG_A Cuts at: 2694 2694 Size: 6778 PmeI GTTT'AAAC Cuts at: 4082 4082 Size: 6778 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4847 4879 6360 6392 4847 Size: 32 1481 32 5233 Fragments arranged by size: 5233 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3336 4060 3336 Size: 724 6054 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6778 PsiI TTA'TAA Cuts at: 4348 4348 Size: 6778 PspOMI G'GGCC_C Cuts at: 3045 3943 3045 Size: 898 5880 PstI C_TGCA'G Cuts at: 830 3255 4130 830 Size: 2425 875 3478 Fragments arranged by size: 3478 2425 875 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6778 PvuII CAG'CTG Cuts at: 2500 2512 3773 3830 2500 Size: 12 1261 57 5448 Fragments arranged by size: 5448 1261 57 12 SacI G_AGCT'C Cuts at: 2290 2556 2862 3195 2290 Size: 266 306 333 5873 Fragments arranged by size: 5873 333 306 266 SalI G'TCGA_C Cuts at: 1936 4120 1936 Size: 2184 4594 SapI GCTCTTCn'nnn_ Cuts at: 2909 2909 Size: 6778 SbfI CC_TGCA'GG Cuts at: 4130 4130 Size: 6778 ScaI AGT'ACT Cuts at: 5026 6738 5026 Size: 1712 5066 SfcI C'TryA_G Cuts at: 826 1045 2507 3251 4126 5261 5942 6133 826 Size: 219 1462 744 875 1135 681 191 1471 Fragments arranged by size: 1471 1462 1135 875 744 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6778 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6778 SmaI CCC'GGG Cuts at: 1872 3371 4105 1872 Size: 1499 734 4545 Fragments arranged by size: 4545 1499 734 SmlI C'TyrA_G Cuts at: 820 1017 1943 2555 3064 3705 4882 5783 6045 Size: 197 926 612 509 641 1177 901 262 Cuts at: 6045 820 Size: 1553 Fragments arranged by size: 1553 1177 926 901 641 612 509 262 197 SnaBI TAC'GTA Cuts at: 493 4569 493 Size: 4076 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6778 SphI G_CATG'C Cuts at: 1132 4136 1132 Size: 3004 3774 SspI AAT'ATT Cuts at: 5 52 4702 5 Size: 47 4650 2081 Fragments arranged by size: 4650 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6778 StyI C'CwwG_G Cuts at: 513 1065 2004 2088 2366 2373 2436 2629 2809 Size: 552 939 84 278 7 63 193 180 Cuts at: 2809 2986 4217 513 Size: 177 1231 3074 Fragments arranged by size: 3074 1231 939 552 278 193 180 177 84 63 7 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4070 4805 4822 4975 5160 5605 325 Size: 1255 2490 735 17 153 185 445 1498 Fragments arranged by size: 2490 1498 1255 735 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2219 2607 2827 Size: 274 80 33 51 1443 242 388 220 Cuts at: 2827 3498 5024 6736 96 Size: 671 1526 1712 138 Fragments arranged by size: 1712 1526 1443 671 388 274 242 220 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2667 2699 6738 6770 2667 Size: 32 4039 32 2675 Fragments arranged by size: 4039 2675 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6778 XbaI T'CTAG_A Cuts at: 4114 4114 Size: 6778 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2089 2380 2089 Size: 291 6487 XhoI C'TCGA_G Cuts at: 1943 2555 3705 1943 Size: 612 1150 5016 Fragments arranged by size: 5016 1150 612 XmaI C'CCGG_G Cuts at: 1870 3369 4103 1870 Size: 1499 734 4545 Fragments arranged by size: 4545 1499 734 XmnI GAAnn'nnTTC Cuts at: 4907 4907 Size: 6778 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4586 276 Size: 53 83 186 936 3052 2468 Fragments arranged by size: 3052 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI