Restriction map for
pFN21AB8293
(Circular) (Six-base) MAPSORT of: pFN21AB8293.seq Check: 1697 from: 1 to: 5611
pFN21AB8293 5611 bp
With 182 enzymes: SgfI *
February 27, 2009 14:42 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3421 278
Size: 53 83 186 936 1885 2468
Fragments arranged by size:
2468 1885 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2518 2954 1937
Size: 581 436 4594
Fragments arranged by size:
4594 581 436
Acc65I G'GTAC_C
Cuts at: 2932 2932
Size: 5611
AclI AA'CG_TT
Cuts at: 1563 2677 3738 4111 1563
Size: 1114 1061 373 3063
Fragments arranged by size:
3063 1114 1061 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3674 5058 5326 1632
Size: 2042 1384 268 1917
Fragments arranged by size:
2042 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 2714 820
Size: 197 1697 3717
Fragments arranged by size:
3717 1697 197
AflIII A'CryG_T
Cuts at: 1203 4510 5487 1203
Size: 3307 977 1327
Fragments arranged by size:
3307 1327 977
AgeI A'CCGG_T
Cuts at: 4442 4442
Size: 5611
AhdI GACnn_n'nnGTC
Cuts at: 4340 4340
Size: 5611
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5611
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5611
AlwNI CAG_nnn'CTG
Cuts at: 2759 3354 4926 2759
Size: 595 1572 3444
Fragments arranged by size:
3444 1572 595
ApaLI G'TGCA_C
Cuts at: 3668 4824 3668
Size: 1156 4455
ApoI r'AATT_y
Cuts at: 1493 2274 2824 2920 3146 3298 5407 1493
Size: 781 550 96 226 152 2109 1697
Fragments arranged by size:
2109 1697 781 550 226 152 96
AseI AT'TA_AT
Cuts at: 160 1032 4165 160
Size: 872 3133 1606
Fragments arranged by size:
3133 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5611
AvaI C'yCGr_G
Cuts at: 1870 1943 2907 2936 1870
Size: 73 964 29 4545
Fragments arranged by size:
4545 964 73 29
BamHI G'GATC_C
Cuts at: 2087 2941 3308 2087
Size: 854 367 4390
Fragments arranged by size:
4390 854 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2932 4387 618
Size: 325 217 627 1145 1455 1842
Fragments arranged by size:
1842 1455 1145 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2174 2930 1391
Size: 783 756 4072
Fragments arranged by size:
4072 783 756
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2700 2738 928
Size: 971 801 38 3801
Fragments arranged by size:
3801 971 801 38
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3802 3836 1903
Size: 34 1865 34 3678
Fragments arranged by size:
3678 1865 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3503 4713 3503
Size: 1210 4401
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5611
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4222 136
Size: 107 122 71 631 605 2550 1525
Fragments arranged by size:
2550 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2260 2623 5606 2260
Size: 363 2983 2265
Fragments arranged by size:
2983 2265 363
BlpI GC'TnA_GC
Cuts at: 3028 3028
Size: 5611
Bme1580I G_kGCm'C
Cuts at: 1790 3672 4828 1790
Size: 1882 1156 2573
Fragments arranged by size:
2573 1882 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5611
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3342 4300 442
Size: 416 53 83 399 24 1925 958 1753
Fragments arranged by size:
1925 1753 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5611
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2798 4271 1239
Size: 1559 1473 2579
Fragments arranged by size:
2579 1559 1473
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2095 3736 4601 4899 2095
Size: 1641 865 298 2807
Fragments arranged by size:
2807 1641 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4274 882
Size: 617 2775 2219
Fragments arranged by size:
2775 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3402 493
Size: 711 2198 2702
Fragments arranged by size:
2702 2198 711
BsaBI GATnn'nnATC
Cuts at: 5605 5605
Size: 5611
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3418 3800 4435 275
Size: 53 83 186 936 1885 382 635 1451
Fragments arranged by size:
1885 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4044 4442 4716 4863 5274 4044
Size: 398 274 147 411 4381
Fragments arranged by size:
4381 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5386
Fragments arranged by size:
5386 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5611
BseYI C'CCAG_C
Cuts at: 4814 5310 4814
Size: 496 5115
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2322 2426 1163
Size: 573 586 104 4348
Fragments arranged by size:
4348 586 573 104
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3061 3822 4850 1540
Size: 275 186 1060 761 1028 2301
Fragments arranged by size:
2301 1060 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2124 3672 3757 4828 2124
Size: 1548 85 1071 2907
Fragments arranged by size:
2907 1548 1071 85
BsmI GAATG_Cn'
Cuts at: 2664 2884 3122 3215 2664
Size: 220 238 93 5060
Fragments arranged by size:
5060 238 220 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2124 2174 2930 3672 3757 4828 1391
Size: 399 334 50 756 742 85 1071 2174
Fragments arranged by size:
2174 1071 756 742 399 334 85 50
BspEI T'CCGG_A
Cuts at: 5274 5274
Size: 5611
BspHI T'CATG_A
Cuts at: 3500 5230 3500
Size: 1730 3881
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2966 844
Size: 2122 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3498 1394
Size: 429 1675 3507
Fragments arranged by size:
3507 1675 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2241 4106 4280 65
Size: 1183 993 1865 174 1396
Fragments arranged by size:
1865 1396 1183 993 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4255 4442 1700
Size: 110 2445 187 2869
Fragments arranged by size:
2869 2445 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5611
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5611
BssSI C'ACGA_G
Cuts at: 3671 4683 3671
Size: 1012 4599
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5611
BstBI TT'CG_AA
Cuts at: 2466 2509 4415 2466
Size: 43 1906 3662
Fragments arranged by size:
3662 1906 43
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5611
BstXI CCAn_nnnn'nTGG
Cuts at: 2776 2776
Size: 5611
BstYI r'GATC_y
Cuts at: 1972 2087 2260 2623 2941 3308 3693 3710 5162
Size: 115 173 363 318 367 385 17 1452
Cuts at: 5162 5248 5260 5606 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 385 367 363 346 318 173
115 86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4120
Fragments arranged by size:
4120 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4872
Fragments arranged by size:
4872 683 56
BtsI GCAGTG_nn'
Cuts at: 2033 3127 3921 3941 2033
Size: 1094 794 20 3703
Fragments arranged by size:
3703 1094 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5611
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5576
DraI TTT'AAA
Cuts at: 2915 2915
Size: 5611
DraIII CAC_nnn'GTG
Cuts at: 2345 2345
Size: 5611
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4618 809
Size: 507 3302 1802
Fragments arranged by size:
3302 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3058 3947 5576
Size: 54 1425 50 275 18 1228 889 1629
Cuts at: 5576 8
Size: 43
Fragments arranged by size:
1629 1425 1228 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 3058 1537
Size: 275 1246 4090
Fragments arranged by size:
4090 1246 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2295 3541 1353
Size: 942 1246 3423
Fragments arranged by size:
3423 1246 942
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2097 4198 4572 4718 387
Size: 1064 138 508 2101 374 146 1280
Fragments arranged by size:
2101 1280 1064 508 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2798 3674 4271 5058 5326 1239
Size: 393 1166 876 597 787 268 1524
Fragments arranged by size:
1524 1166 876 787 597 393 268
EcoRI G'AATT_C
Cuts at: 2920 3298 2920
Size: 378 5233
FspI TGC'GCA
Cuts at: 4117 4117
Size: 5611
HaeII r_GCGC'y
Cuts at: 1678 4758 1678
Size: 3080 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2453 2485 2600 2632 4252
Size: 32 135 32 881 32 115 32 1620
Cuts at: 4252 4284 4326 4358 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1620 881 135 115 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2215 2955 3201 669
Size: 988 281 277 740 246 3079
Fragments arranged by size:
3079 988 740 281 277 246
HindIII A'AGCT_T
Cuts at: 748 2325 748
Size: 1577 4034
HpaI GTT'AAC
Cuts at: 3201 3201
Size: 5611
KpnI G_GTAC'C
Cuts at: 2936 2936
Size: 5611
MfeI C'AATT_G
Cuts at: 3210 3210
Size: 5611
MluI A'CGCG_T
Cuts at: 5487 5487
Size: 5611
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2448 4725 4909 1305
Size: 1143 2277 184 2007
Fragments arranged by size:
2277 2007 1143 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3789
Fragments arranged by size:
3789 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3569 3928 4087 518
Size: 648 2403 359 159 2042
Fragments arranged by size:
2403 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3027 3704 4852 5097 1606
Size: 1421 677 1148 245 2120
Fragments arranged by size:
2120 1421 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 4120
Fragments arranged by size:
4120 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5611
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5611
NotI GC'GGCC_GC
Cuts at: 3058 3058
Size: 5611
NspI r_CATG'y
Cuts at: 1132 1229 2109 2969 4514 1132
Size: 97 880 860 1545 2229
Fragments arranged by size:
2229 1545 880 860 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5611
PciI A'CATG_T
Cuts at: 4510 4510
Size: 5611
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5611
PmeI GTTT'AAAC
Cuts at: 2915 2915
Size: 5611
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2662 2694 3680 3712 5193 5225 2662
Size: 32 986 32 1481 32 3048
Fragments arranged by size:
3048 1481 986 32 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5611
PsiI TTA'TAA
Cuts at: 3181 3181
Size: 5611
PspXI vC'TCGA_Gb
Cuts at: 2907 2907
Size: 5611
PstI C_TGCA'G
Cuts at: 830 2067 2963 830
Size: 1237 896 3478
Fragments arranged by size:
3478 1237 896
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5611
SalI G'TCGA_C
Cuts at: 1936 2953 1936
Size: 1017 4594
SbfI CC_TGCA'GG
Cuts at: 2963 2963
Size: 5611
ScaI AGT'ACT
Cuts at: 3859 5571 3859
Size: 1712 3899
SfcI C'TryA_G
Cuts at: 826 1045 2063 2535 2959 4094 4775 4966 826
Size: 219 1018 472 424 1135 681 191 1471
Fragments arranged by size:
1471 1135 1018 681 472 424 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5611
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5611
SmaI CCC'GGG
Cuts at: 1872 2938 1872
Size: 1066 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2110 2714 2907 3715 4616 4878
Size: 197 926 167 604 193 808 901 262
Cuts at: 4878 820
Size: 1553
Fragments arranged by size:
1553 926 901 808 604 262 197 193
167
SnaBI TAC'GTA
Cuts at: 493 3402 493
Size: 2909 2702
SpeI A'CTAG_T
Cuts at: 152 2594 152
Size: 2442 3169
SphI G_CATG'C
Cuts at: 1132 2969 1132
Size: 1837 3774
SspI AAT'ATT
Cuts at: 5 52 3535 5
Size: 47 3483 2081
Fragments arranged by size:
3483 2081 47
StuI AGG'CCT
Cuts at: 1517 2191 1517
Size: 674 4937
StyI C'CwwG_G
Cuts at: 513 1065 2004 3050 513
Size: 552 939 1046 3074
Fragments arranged by size:
3074 1046 939 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3638 3655 3808 3993 4438 325
Size: 1255 2058 17 153 185 445 1498
Fragments arranged by size:
2058 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3857 5569 96
Size: 274 80 33 51 1443 1880 1712 138
Fragments arranged by size:
1880 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5571 5603 5571
Size: 32 5579
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5611
XbaI T'CTAG_A
Cuts at: 2626 2947 2626
Size: 321 5290
XhoI C'TCGA_G
Cuts at: 1943 2907 1943
Size: 964 4647
XmaI C'CCGG_G
Cuts at: 1870 2936 1870
Size: 1066 4545
XmnI GAAnn'nnTTC
Cuts at: 2505 2697 3740 2505
Size: 192 1043 4376
Fragments arranged by size:
4376 1043 192
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3419 276
Size: 53 83 186 936 1885 2468
Fragments arranged by size:
2468 1885 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PmeI
PpiI PshAI PsiI PspXI PstI PvuI SalI SbfI
ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI
PpuMI PspOMI PsrI PvuII RsrII SacI SacII SanDI
SapI SexAI SfiI SfoI SrfI SwaI XcmI