Restriction map for
pFN21AB8292
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AocI
Aor51HI ApaI AscI AseI AsnI
Asp700I Asp718I AspI AviII AvrII
BalI BamHI BanI BanII BbeI
BbrPI BbvCI BcgI BcnI BfaI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BseRI BsiEI BsiWI BsmI BspDI
BspEI BsrBI BsrDI BsrFI BssHII
Bst1107I BstAPI BstBI BstUI BstYI
BstZ17I Bsu36I CelII CfoI Cfr10I
ClaI CpoI DraIII DrdI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRI EcoRV EcoT22I FauI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HpaII KasI KpnI
KspI MaeI MamI MflI MluI
MluNI MroI MscI MspI MvnI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI TaqI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AfaI 279 GTAC GT^AC
Alw44I 927 GTGCAC G^TGCAC
AlwNI 1321 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 927 GTGCAC G^TGCAC
AvaI 546 CCCGAG C^[CT]CG[GA]G
BciVI 347 GTATCC GTATCC(6/5)
BclI 48 TGATCA T^GATCA
BglI 832 GCCTGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 1327 CTGGAG CTGGAG(16/14)
BsaI 670 GAGACC GGTCTC(1/5)
BsgI 1179 CTGCAC GTGCAG(16/14)
BsmBI 1330 GAGACG CGTCTC(1/5)
BsoBI 546 CCCGAG C^[CT]CG[GA]G
Bsp1407I 278 TGTACA T^GTACA
BspHI 1134 TCATGA T^CATGA
BspMI 1248 GCAGGT ACCTGC(4/8)
BsrGI 278 TGTACA T^GTACA
BssSI 1219 CACGAG CACGAG(-5/-1)
BstXI 837 CCAGGCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 181 TTTAAA TTT^AAA
DsaI 665 CCATGG C^C[GA][CT]GG
EaeI 648 CGGCCA [CT]^GGCC[GA]
EarI 259 GAAGAG CTCTTC(1/4)
FbaI 48 TGATCA T^GATCA
HincII 313 GTTAAC GT[CT]^[GA]AC
HindII 313 GTTAAC GT[CT]^[GA]AC
HpaI 313 GTTAAC GTT^AAC
Ksp632I 259 GAAGAG CTCTTC(1/4)
MaeII 853 ACGT A^CGT
MfeI 639 CAATTG C^AATTG
MspA1I 1324 CAGCTG C[AC]G^C[GT]G
MunI 639 CAATTG C^AATTG
NcoI 665 CCATGG C^CATGG
PleI 549 GAGTC GAGTC(4/5)
PstI 168 CTGCAG CTGCA^G
PvuII 1324 CAGCTG CAG^CTG
RcaI 1134 TCATGA T^CATGA
RsaI 279 GTAC GT^AC
SspBI 278 TGTACA T^GTACA
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 100 GAATTT [GA]^AATT[CT]
136 AAATTC
AflIII 275 ACATGT A^C[GA][CT]GT
1091 ACATGT
AhdI 559 GACGGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
688 GACTTGGGGTC
ApoI 100 GAATTT [GA]^AATT[CT]
136 AAATTC
AspEI 559 GACGGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
688 GACTTGGGGTC
AspHI 143 GAGCAC G[AT]GC[AT]^C
927 GTGCAC
AvaII 264 GGTCC G^G[AT]CC
695 GGTCC
BbsI 849 GAAGAC GAAGAC(2/6)
1142 GAAGAC
BpuAI 849 GAAGAC GAAGAC(2/6)
1142 GAAGAC
BsiHKAI 143 GAGCAC G[AT]GC[AT]^C
927 GTGCAC
BsmFI 395 GTCCC GGGAC(10/14)
696 GTCCC
BspLU11I 275 ACATGT A^CATGT
1091 ACATGT
BstEII 811 GGTCACC G^GT[ACGT]ACC
1251 GGTTACC
BstPI 811 GGTCACC G^GT[ACGT]ACC
1251 GGTTACC
DdeI 374 CTCAG C^T[ACGT]AG
583 CTCAG
DraII 263 AGGTCCT [GA]G^G[ACGT]CC[CT]
694 GGGTCCC
Eam1105I 559 GACGGCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
688 GACTTGGGGTC
EcoO109I 263 AGGTCCT [GA]G^G[ACGT]CC[CT]
694 GGGTCCC
EcoO65I 811 GGTCACC G^GT[ACGT]ACC
1251 GGTTACC
HindIII 187 AAGCTT A^AGCTT
199 AAGCTT
NspI 275 ACATGT [GA]CATG^[CT]
1091 ACATGT
PpuMI 263 AGGTCCT [GA]G^G[AT]CC[CT]
694 GGGTCCC
SfaNI 460 GATGC GCATC(5/9)
1201 GCATC
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AluI AlwI BbvI BmyI
BsaJI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DpnII EcoRII EcoT14I Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeIII MboI MboII MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfcI
SmlI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI