(Circular) (Six-base) MAPSORT of: pFN21AB8292.seq Check: 4691 from: 1 to: 6052 pFN21AB8292 6052 bp With 182 enzymes: SgfI * February 27, 2009 14:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3862 278 Size: 53 83 186 936 2326 2468 Fragments arranged by size: 2468 2326 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3395 1937 Size: 1458 4594 Acc65I G'GTAC_C Cuts at: 3373 3373 Size: 6052 AclI AA'CG_TT Cuts at: 1563 4179 4552 1563 Size: 2616 373 3063 Fragments arranged by size: 3063 2616 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4115 5499 5767 1632 Size: 2483 1384 268 1917 Fragments arranged by size: 2483 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5855 AflIII A'CryG_T Cuts at: 1203 2280 3096 4951 5928 1203 Size: 1077 816 1855 977 1327 Fragments arranged by size: 1855 1327 1077 977 816 AgeI A'CCGG_T Cuts at: 4883 4883 Size: 6052 AhdI GACnn_n'nnGTC Cuts at: 2569 2698 4781 2569 Size: 129 2083 3840 Fragments arranged by size: 3840 2083 129 AleI CACnn'nnGTG Cuts at: 1166 2157 3099 1166 Size: 991 942 4119 Fragments arranged by size: 4119 991 942 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6052 AlwNI CAG_nnn'CTG Cuts at: 3331 3795 5367 3331 Size: 464 1572 4016 Fragments arranged by size: 4016 1572 464 ApaLI G'TGCA_C Cuts at: 2932 4109 5265 2932 Size: 1177 1156 3719 Fragments arranged by size: 3719 1177 1156 ApoI r'AATT_y Cuts at: 1493 2105 2141 3361 3587 3739 5848 1493 Size: 612 36 1220 226 152 2109 1697 Fragments arranged by size: 2109 1697 1220 612 226 152 36 AseI AT'TA_AT Cuts at: 160 1032 4606 160 Size: 872 3574 1606 Fragments arranged by size: 3574 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6052 AvaI C'yCGr_G Cuts at: 1870 1943 2551 3377 1870 Size: 73 608 826 4545 Fragments arranged by size: 4545 826 608 73 BamHI G'GATC_C Cuts at: 3382 3749 3382 Size: 367 5685 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3373 4828 618 Size: 325 217 627 1586 1455 1842 Fragments arranged by size: 1842 1586 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3371 1391 Size: 1980 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2861 3154 928 Size: 971 962 293 3826 Fragments arranged by size: 3826 971 962 293 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4243 4277 1903 Size: 34 2306 34 3678 Fragments arranged by size: 3678 2306 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2363 3944 5154 2363 Size: 1581 1210 3261 Fragments arranged by size: 3261 1581 1210 BclI T'GATC_A Cuts at: 1548 2053 1548 Size: 505 5547 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2843 4663 136 Size: 107 122 71 631 605 1171 1820 1525 Fragments arranged by size: 1820 1525 1171 631 605 122 107 71 BglII A'GATC_T Cuts at: 6047 6047 Size: 6052 BlpI GC'TnA_GC Cuts at: 3469 3469 Size: 6052 Bme1580I G_kGCm'C Cuts at: 1790 2936 3040 4113 5269 1790 Size: 1146 104 1073 1156 2573 Fragments arranged by size: 2573 1156 1146 1073 104 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6052 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3258 3783 4741 Size: 416 53 83 399 24 1841 525 958 Cuts at: 4741 442 Size: 1753 Fragments arranged by size: 1841 1753 958 525 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6052 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3353 4712 1239 Size: 2114 1359 2579 Fragments arranged by size: 2579 2114 1359 Bpu10I CC'TnA_GC Cuts at: 2588 2588 Size: 6052 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2228 2697 3116 4177 5042 5340 2228 Size: 469 419 1061 865 298 2940 Fragments arranged by size: 2940 1061 865 469 419 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2669 4715 882 Size: 617 1170 2046 2219 Fragments arranged by size: 2219 2046 1170 617 BsaAI yAC'GTr Cuts at: 493 1204 3843 493 Size: 711 2639 2702 Fragments arranged by size: 2702 2639 711 BsaBI GATnn'nnATC Cuts at: 6046 6046 Size: 6052 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3859 4241 4876 275 Size: 53 83 186 936 2326 382 635 1451 Fragments arranged by size: 2326 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4485 4883 5157 5304 5715 4485 Size: 398 274 147 411 4822 Fragments arranged by size: 4822 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5827 Fragments arranged by size: 5827 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6052 BseYI C'CCAG_C Cuts at: 2617 5255 5751 2617 Size: 2638 496 2918 Fragments arranged by size: 2918 2638 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3169 1163 Size: 573 1433 4046 Fragments arranged by size: 4046 1433 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3502 4263 5291 1540 Size: 275 186 1501 761 1028 2301 Fragments arranged by size: 2301 1501 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2152 2936 4113 4198 5269 2152 Size: 784 1177 85 1071 2935 Fragments arranged by size: 2935 1177 1071 784 85 BsmI GAATG_Cn' Cuts at: 3563 3656 3563 Size: 93 5959 BsmBI CGTCTCn'nnnn_ Cuts at: 3329 3329 Size: 6052 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2152 2936 3040 3371 4113 4198 5269 Size: 399 362 784 104 331 742 85 1071 Cuts at: 5269 1391 Size: 2174 Fragments arranged by size: 2174 1071 784 742 399 362 331 104 85 BspEI T'CCGG_A Cuts at: 5715 5715 Size: 6052 BspHI T'CATG_A Cuts at: 3139 3941 5671 3139 Size: 802 1730 3520 Fragments arranged by size: 3520 1730 802 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3244 3407 844 Size: 2400 163 3489 Fragments arranged by size: 3489 2400 163 BsrBI CCG'CTC Cuts at: 1394 1823 3939 1394 Size: 429 2116 3507 Fragments arranged by size: 3507 2116 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4547 4721 65 Size: 1183 3299 174 1396 Fragments arranged by size: 3299 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4696 4883 1700 Size: 110 2886 187 2869 Fragments arranged by size: 2886 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2283 96 Size: 2187 3865 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6052 BssSI C'ACGA_G Cuts at: 3224 4112 5124 3224 Size: 888 1012 4152 Fragments arranged by size: 4152 1012 888 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6052 BstBI TT'CG_AA Cuts at: 4856 4856 Size: 6052 BstEII G'GTnAC_C Cuts at: 1184 2816 3256 1184 Size: 1632 440 3980 Fragments arranged by size: 3980 1632 440 BstXI CCAn_nnnn'nTGG Cuts at: 2849 2849 Size: 6052 BstYI r'GATC_y Cuts at: 1972 3382 3749 4134 4151 5603 5689 5701 6047 Size: 1410 367 385 17 1452 86 12 346 Cuts at: 6047 1972 Size: 1977 Fragments arranged by size: 1977 1452 1410 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2670 513 Size: 552 939 666 3895 Fragments arranged by size: 3895 939 666 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3320 487 Size: 683 56 2094 3219 Fragments arranged by size: 3219 2094 683 56 BtsI GCAGTG_nn' Cuts at: 3568 4362 4382 3568 Size: 794 20 5238 Fragments arranged by size: 5238 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6052 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6017 DraI TTT'AAA Cuts at: 2188 3356 2188 Size: 1168 4884 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5059 809 Size: 507 3743 1802 Fragments arranged by size: 3743 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2653 3499 4388 Size: 54 1425 50 275 18 823 846 889 Cuts at: 4388 6017 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 846 823 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3499 1537 Size: 275 1687 4090 Fragments arranged by size: 4090 1687 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2259 3982 1353 Size: 906 1723 3423 Fragments arranged by size: 3423 1723 906 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2921 4639 5013 5159 387 Size: 1064 138 1332 1718 374 146 1280 Fragments arranged by size: 1718 1332 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3353 4115 4712 5499 5767 1239 Size: 393 1721 762 597 787 268 1524 Fragments arranged by size: 1721 1524 787 762 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2269 2700 2269 Size: 431 5621 EcoRI G'AATT_C Cuts at: 3361 3739 3361 Size: 378 5674 FspI TGC'GCA Cuts at: 4558 4558 Size: 6052 HaeII r_GCGC'y Cuts at: 1678 5199 1678 Size: 3521 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2555 2587 2684 2716 4693 Size: 32 135 32 983 32 97 32 1977 Cuts at: 4693 4725 4767 4799 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1977 983 135 97 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2320 3396 3642 669 Size: 988 281 382 1076 246 3079 Fragments arranged by size: 3079 1076 988 382 281 246 HindIII A'AGCT_T Cuts at: 748 2192 2204 748 Size: 1444 12 4596 Fragments arranged by size: 4596 1444 12 HpaI GTT'AAC Cuts at: 2320 3642 2320 Size: 1322 4730 KpnI G_GTAC'C Cuts at: 3377 3377 Size: 6052 MfeI C'AATT_G Cuts at: 2644 3651 2644 Size: 1007 5045 MluI A'CGCG_T Cuts at: 5928 5928 Size: 6052 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5166 5350 1305 Size: 3861 184 2007 Fragments arranged by size: 3861 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4230 Fragments arranged by size: 4230 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2021 2155 2157 3072 3099 4010 4369 Size: 648 855 134 2 915 27 911 359 Cuts at: 4369 4528 518 Size: 159 2042 Fragments arranged by size: 2042 915 911 855 648 359 159 134 27 2 MspA1I CmG'CkG Cuts at: 1606 3331 3468 4145 5293 5538 1606 Size: 1725 137 677 1148 245 2120 Fragments arranged by size: 2120 1725 1148 677 245 137 NcoI C'CATG_G Cuts at: 513 1065 2004 2670 513 Size: 552 939 666 3895 Fragments arranged by size: 3895 939 666 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6052 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6052 NotI GC'GGCC_GC Cuts at: 3499 3499 Size: 6052 NspI r_CATG'y Cuts at: 1132 1229 2284 3100 3410 4955 1132 Size: 97 1055 816 310 1545 2229 Fragments arranged by size: 2229 1545 1055 816 310 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6052 PciI A'CATG_T Cuts at: 2280 3096 4951 2280 Size: 816 1855 3381 Fragments arranged by size: 3381 1855 816 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6052 PmeI GTTT'AAAC Cuts at: 3356 3356 Size: 6052 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4121 4153 5634 5666 4121 Size: 32 1481 32 4507 Fragments arranged by size: 4507 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2269 2700 2269 Size: 431 5621 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6052 PsiI TTA'TAA Cuts at: 3622 3622 Size: 6052 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2267 2299 2267 Size: 32 6020 PstI C_TGCA'G Cuts at: 830 2177 3404 830 Size: 1347 1227 3478 Fragments arranged by size: 3478 1347 1227 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6052 PvuII CAG'CTG Cuts at: 3331 3331 Size: 6052 SalI G'TCGA_C Cuts at: 1936 3394 1936 Size: 1458 4594 SanDI GG'GwC_CC Cuts at: 2700 2700 Size: 6052 SbfI CC_TGCA'GG Cuts at: 3404 3404 Size: 6052 ScaI AGT'ACT Cuts at: 4300 6012 4300 Size: 1712 4340 SfcI C'TryA_G Cuts at: 826 1045 2173 3001 3346 3400 4535 5216 5407 Size: 219 1128 828 345 54 1135 681 191 Cuts at: 5407 826 Size: 1471 Fragments arranged by size: 1471 1135 1128 828 681 345 219 191 54 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6052 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6052 SmaI CCC'GGG Cuts at: 1872 3379 1872 Size: 1507 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2207 2712 3131 4156 5057 5319 Size: 197 926 264 505 419 1025 901 262 Cuts at: 5319 820 Size: 1553 Fragments arranged by size: 1553 1025 926 901 505 419 264 262 197 SnaBI TAC'GTA Cuts at: 493 3843 493 Size: 3350 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6052 SphI G_CATG'C Cuts at: 1132 3410 1132 Size: 2278 3774 SspI AAT'ATT Cuts at: 5 52 3976 5 Size: 47 3924 2081 Fragments arranged by size: 3924 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6052 StyI C'CwwG_G Cuts at: 513 1065 2004 2670 3189 3201 3491 513 Size: 552 939 666 519 12 290 3074 Fragments arranged by size: 3074 939 666 552 519 290 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2215 2492 4079 4096 4249 4434 4879 Size: 1255 635 277 1587 17 153 185 445 Cuts at: 4879 325 Size: 1498 Fragments arranged by size: 1587 1498 1255 635 445 277 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2283 4298 6010 Size: 274 80 33 51 1443 306 2015 1712 Cuts at: 6010 96 Size: 138 Fragments arranged by size: 2015 1712 1443 306 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2357 2389 6012 6044 2357 Size: 32 3623 32 2365 Fragments arranged by size: 3623 2365 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6052 XbaI T'CTAG_A Cuts at: 3388 3388 Size: 6052 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6052 XmaI C'CCGG_G Cuts at: 1870 3377 1870 Size: 1507 4545 XmnI GAAnn'nnTTC Cuts at: 4181 4181 Size: 6052 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3860 276 Size: 53 83 186 936 2326 2468 Fragments arranged by size: 2468 2326 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PsrI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI