Restriction map for
pFN21AB8291
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AfaI AflII AflIII AhdI Alw44I
AocI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BamHI BbeI BbrPI
BbsI BcgI BciVI BclI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspHI BspLU11I BsrDI BsrGI
BssHII BssSI BstBI BstUI Bsu36I
CelII ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco52I
Eco81I EcoNI EcoRV EcoT22I FbaI
FseI FspI Hin1I HindIII KasI
KpnI KspI MaeII MamI MfeI
MluI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsaI RsrII SacI
SacII SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI Tth111I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 34 enzymes) #
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AccIII 619 TCCGGA T^CCGGA
AcsI 600 GAATTC [GA]^AATT[CT]
AgeI 735 ACCGGT A^CCGGT
Aor51HI 663 AGCGCT AGC^GCT
ApaI 544 GGGCCC GGGCC^C
ApoI 600 GAATTC [GA]^AATT[CT]
AspHI 355 GAGCAC G[AT]GC[AT]^C
BcnI 1025 CCCGG CC^[GC]GG
BfaI 538 CTAG C^TAG
BglI 1237 GCCACCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseAI 619 TCCGGA T^CCGGA
BsgI 1268 CTGCAC GTGCAG(16/14)
BsiHKAI 355 GAGCAC G[AT]GC[AT]^C
BspEI 619 TCCGGA T^CCGGA
Bst1107I 220 GTATAC GTA^TAC
BstXI 1196 CCATCAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 220 GTATAC GTA^TAC
Eco47III 663 AGCGCT AGC^GCT
EcoRI 600 GAATTC G^AATTC
HaeII 663 AGCGCT [GA]GCGC^[CT]
HgaI 249 GACGC GACGC(5/10)
HpaI 817 GTTAAC GTT^AAC
MaeI 538 CTAG C^TAG
MroI 619 TCCGGA T^CCGGA
MseI 818 TTAA T^TAA
NciI 1025 CCCGG CC^[GC]GG
PflMI 429 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 735 ACCGGT A^CCGGT
SalI 1354 GTCGAC G^TCGAC
SapI 918 GCTCTTC GCTCTTC(1/4)
SphI 891 GCATGC GCATG^C
Tru9I 818 TTAA T^TAA
Van91I 429 CCACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 308 CCAGCTTTGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccI 220 GTATAC GT^[AC][GT]AC
1354 GTCGAC
AlwI 801 GATCC GGATC(4/5)
1069 GATCC
BalI 41 TGGCCA TGG^CCA
1244 TGGCCA
BanI 227 GGCACC G^G[CT][GA]CC
1168 GGTGCC
BanII 285 GGGCTC G[GA]GC[CT]^C
544 GGGCCC
BbvCI 143 CCTCAGC CCTCAGC(-5/-2)
659 CCTCAGC
BsaWI 619 TCCGGA [AT]^CCGG[AT]
735 ACCGGT
BsmAI 625 GTCTC GTCTC(1/5)
1098 GTCTC
BsrBI 26 CCGCTC CCGCTC(-3/-3)
241 CCGCTC
BsrFI 735 ACCGGT [GA]^CCGG[CT]
1106 ACCGGC
BstAPI 927 GCATCTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1041 GCAGCCCATGC
BstYI 800 AGATCC [GA]^GATC[CT]
1068 AGATCC
CfoI 664 GCGC GCG^C
1032 GCGC
Cfr10I 735 ACCGGT [GA]^CCGG[CT]
1106 ACCGGC
EaeI 41 TGGCCA [CT]^GGCC[GA]
1244 TGGCCA
HhaI 664 GCGC GCG^C
1032 GCGC
HinP1I 664 GCGC G^CGC
1032 GCGC
MflI 800 AGATCC [GA]^GATC[CT]
1068 AGATCC
MluNI 41 TGGCCA TGG^CCA
1244 TGGCCA
MscI 41 TGGCCA TGG^CCA
1244 TGGCCA
MspA1I 146 CAGCTG C[AC]G^C[GT]G
196 CAGCTG
NcoI 743 CCATGG C^CATGG
1241 CCATGG
NspI 9 ACATGC [GA]CATG^[CT]
891 GCATGC
PpuMI 416 AGGTCCT [GA]G^G[AT]CC[CT]
587 AGGACCT
PshAI 690 GACAACCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1371 GACAAATGTC
PvuII 146 CAGCTG CAG^CTG
196 CAGCTG
TfiI 425 GAATC G^A[AT]TC
1121 GATTC
Tsp45I 769 GTCAC ^GT[GC]AC
1403 GTGAC
Tsp509I 601 AATT ^AATT
610 AATT
XhoII 800 AGATCC [GA]^GATC[CT]
1068 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AluI AlwNI AvaII BbvI
BmyI BpmI BsaJI BseRI BsiYI
BslI BsmFI Bsp1286I BspMI BsrI
BssKI BstEII BstF5I BstNI BstPI
Cac8I Cfr13I DdeI DpnII DraII
DsaI EarI EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeIII MboI
MboII MnlI MslI MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
PstI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TseI TspRI
TthHB8I