(Circular) (Six-base) MAPSORT of: pFN21AB8291.seq Check: 3285 from: 1 to: 6121 pFN21AB8291 6121 bp With 182 enzymes: SgfI * February 27, 2009 14:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2445 2504 2445 Size: 59 6062 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3931 278 Size: 53 83 186 936 2395 2468 Fragments arranged by size: 2468 2395 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2226 3360 3464 1937 Size: 289 1134 104 4594 Fragments arranged by size: 4594 1134 289 104 Acc65I G'GTAC_C Cuts at: 3442 3442 Size: 6121 AclI AA'CG_TT Cuts at: 1563 4248 4621 1563 Size: 2685 373 3063 Fragments arranged by size: 3063 2685 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2359 4184 5568 5836 1632 Size: 727 1825 1384 268 1917 Fragments arranged by size: 1917 1825 1384 727 268 AfeI AGC'GCT Cuts at: 2670 2670 Size: 6121 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5924 AflIII A'CryG_T Cuts at: 1203 5020 5997 1203 Size: 3817 977 1327 Fragments arranged by size: 3817 1327 977 AgeI A'CCGG_T Cuts at: 2740 4952 2740 Size: 2212 3909 AhdI GACnn_n'nnGTC Cuts at: 4850 4850 Size: 6121 AleI CACnn'nnGTG Cuts at: 1166 2168 1166 Size: 1002 5119 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2186 3189 1184 Size: 1002 1003 4116 Fragments arranged by size: 4116 1003 1002 AlwNI CAG_nnn'CTG Cuts at: 2156 2425 3077 3226 3864 5436 2156 Size: 269 652 149 638 1572 2841 Fragments arranged by size: 2841 1572 652 638 269 149 ApaI G_GGCC'C Cuts at: 2553 2553 Size: 6121 ApaLI G'TGCA_C Cuts at: 4178 5334 4178 Size: 1156 4965 ApoI r'AATT_y Cuts at: 1493 2605 3430 3656 3808 5917 1493 Size: 1112 825 226 152 2109 1697 Fragments arranged by size: 2109 1697 1112 825 226 152 AseI AT'TA_AT Cuts at: 160 1032 4675 160 Size: 872 3643 1606 Fragments arranged by size: 3643 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6121 AvaI C'yCGr_G Cuts at: 1870 1943 3446 1870 Size: 73 1503 4545 Fragments arranged by size: 4545 1503 73 BamHI G'GATC_C Cuts at: 3451 3818 3451 Size: 367 5754 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2232 3173 3442 4897 618 Size: 325 217 627 445 941 269 1455 1842 Fragments arranged by size: 1842 1455 941 627 445 325 269 217 BanII G_rGCy'C Cuts at: 1391 2294 2553 3440 1391 Size: 903 259 887 4072 Fragments arranged by size: 4072 903 887 259 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5150 BbvCI CC'TCA_GC Cuts at: 2149 2665 2149 Size: 516 5605 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4312 4346 1903 Size: 34 2375 34 3678 Fragments arranged by size: 3678 2375 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4013 5223 4013 Size: 1210 4911 BclI T'GATC_A Cuts at: 1548 1548 Size: 6121 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3248 4732 136 Size: 107 122 71 631 605 1576 1484 1525 Fragments arranged by size: 1576 1525 1484 631 605 122 107 71 BglII A'GATC_T Cuts at: 6116 6116 Size: 6121 BlpI GC'TnA_GC Cuts at: 3538 3538 Size: 6121 Bme1580I G_kGCm'C Cuts at: 1790 2175 2182 2553 3178 4182 5338 1790 Size: 385 7 371 625 1004 1156 2573 Fragments arranged by size: 2573 1156 1004 625 385 371 7 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6121 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2169 3852 4810 Size: 416 53 83 399 24 752 1683 958 Cuts at: 4810 442 Size: 1753 Fragments arranged by size: 1753 1683 958 752 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6121 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2225 2462 2468 2657 3368 3437 4781 1239 Size: 986 237 6 189 711 69 1344 2579 Fragments arranged by size: 2579 1344 986 711 237 189 69 6 Bpu10I CC'TnA_GC Cuts at: 2149 2531 2665 2149 Size: 382 134 5605 Fragments arranged by size: 5605 382 134 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4246 5111 5409 4246 Size: 865 298 4958 Fragments arranged by size: 4958 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4784 882 Size: 617 3285 2219 Fragments arranged by size: 3285 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3912 493 Size: 711 2708 2702 Fragments arranged by size: 2708 2702 711 BsaBI GATnn'nnATC Cuts at: 6115 6115 Size: 6121 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3928 4310 4945 275 Size: 53 83 186 936 2395 382 635 1451 Fragments arranged by size: 2395 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2624 2740 4554 4952 5226 5373 5784 2624 Size: 116 1814 398 274 147 411 2961 Fragments arranged by size: 2961 1814 411 398 274 147 116 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2555 2580 2585 2610 2822 Size: 30 165 30 1152 25 5 25 212 Cuts at: 2822 2852 3395 3425 1178 Size: 30 543 30 3874 Fragments arranged by size: 3874 1152 543 212 165 30 30 30 30 25 25 5 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2895 3344 3347 3350 3353 3356 3365 3368 Size: 1709 449 3 3 3 3 9 3 Cuts at: 3368 1186 Size: 3939 Fragments arranged by size: 3939 1709 449 9 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 2023 2106 2199 2659 5324 5820 2023 Size: 83 93 460 2665 496 2324 Fragments arranged by size: 2665 2324 496 460 93 83 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3258 1163 Size: 573 1522 4026 Fragments arranged by size: 4026 1522 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3571 4332 5360 1540 Size: 275 186 1570 761 1028 2301 Fragments arranged by size: 2301 1570 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2364 4182 4267 5338 2364 Size: 1818 85 1071 3147 Fragments arranged by size: 3147 1818 1071 85 BsmI GAATG_Cn' Cuts at: 3632 3725 3632 Size: 93 6028 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2175 2182 2294 2364 2553 3178 3440 Size: 399 385 7 112 70 189 625 262 Cuts at: 3440 4182 4267 5338 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 625 399 385 262 189 112 85 70 7 BspEI T'CCGG_A Cuts at: 2624 5784 2624 Size: 3160 2961 BspHI T'CATG_A Cuts at: 4010 5740 4010 Size: 1730 4391 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2445 2504 2535 3225 3476 844 Size: 1601 59 31 690 251 3489 Fragments arranged by size: 3489 1601 690 251 59 31 BsrBI CCG'CTC Cuts at: 1394 1823 2033 2248 4008 1394 Size: 429 210 215 1760 3507 Fragments arranged by size: 3507 1760 429 215 210 BsrDI GCAATG_nn' Cuts at: 65 1248 4616 4790 65 Size: 1183 3368 174 1396 Fragments arranged by size: 3368 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2740 3111 4765 4952 1700 Size: 110 930 371 1654 187 2869 Fragments arranged by size: 2869 1654 930 371 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6121 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6121 BssSI C'ACGA_G Cuts at: 4181 5193 4181 Size: 1012 5109 BstAPI GCAn_nnn'nTGC Cuts at: 794 2938 3052 794 Size: 2144 114 3863 Fragments arranged by size: 3863 2144 114 BstBI TT'CG_AA Cuts at: 4925 4925 Size: 6121 BstEII G'GTnAC_C Cuts at: 1184 2743 2752 2773 1184 Size: 1559 9 21 4532 Fragments arranged by size: 4532 1559 21 9 BstXI CCAn_nnnn'nTGG Cuts at: 3208 3208 Size: 6121 BstYI r'GATC_y Cuts at: 1972 2805 3073 3451 3818 4203 4220 5672 5758 Size: 833 268 378 367 385 17 1452 86 Cuts at: 5758 5770 6116 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 833 385 378 367 346 268 86 17 12 BstZ17I GTA'TAC Cuts at: 2227 2227 Size: 6121 BtgI C'CryG_G Cuts at: 513 1065 2748 2952 3246 513 Size: 552 1683 204 294 3388 Fragments arranged by size: 3388 1683 552 294 204 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2394 2671 3127 487 Size: 683 56 1168 277 456 3481 Fragments arranged by size: 3481 1168 683 456 277 56 BtsI GCAGTG_nn' Cuts at: 3637 4431 4451 3637 Size: 794 20 5307 Fragments arranged by size: 5307 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6121 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6086 DraI TTT'AAA Cuts at: 3425 3425 Size: 6121 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5128 809 Size: 507 3812 1802 Fragments arranged by size: 3812 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2046 3249 3568 Size: 54 1425 50 275 18 216 1203 319 Cuts at: 3568 4457 6086 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1203 889 319 275 216 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3568 1537 Size: 275 1756 4090 Fragments arranged by size: 4090 1756 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2930 2960 3300 3321 4051 1353 Size: 1577 30 340 21 730 3423 Fragments arranged by size: 3423 1577 730 340 30 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2299 4708 5082 5228 387 Size: 1064 138 710 2409 374 146 1280 Fragments arranged by size: 2409 1280 1064 710 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2225 2359 2462 2468 2657 3368 3437 Size: 393 593 134 103 6 189 711 69 Cuts at: 3437 4184 4781 5568 5836 1239 Size: 747 597 787 268 1524 Fragments arranged by size: 1524 787 747 711 597 593 393 268 189 134 103 69 6 EcoO109I rG'GnC_Cy Cuts at: 2422 2549 2550 2593 2422 Size: 127 1 43 5950 Fragments arranged by size: 5950 127 43 1 EcoRI G'AATT_C Cuts at: 2605 3430 3808 2605 Size: 825 378 4918 Fragments arranged by size: 4918 825 378 FspI TGC'GCA Cuts at: 4627 4627 Size: 6121 HaeII r_GCGC'y Cuts at: 1678 2672 5268 1678 Size: 994 2596 2531 Fragments arranged by size: 2596 2531 994 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2822 2849 2854 2881 3088 Size: 32 135 32 1250 27 5 27 207 Cuts at: 3088 3120 4762 4794 4836 4868 1373 Size: 32 1642 32 42 32 2626 Fragments arranged by size: 2626 1642 1250 207 135 42 32 32 32 32 32 27 27 5 HincII GTy'rAC Cuts at: 669 1657 1938 2704 2824 3361 3465 3711 669 Size: 988 281 766 120 537 104 246 3079 Fragments arranged by size: 3079 988 766 537 281 246 120 104 HindIII A'AGCT_T Cuts at: 748 748 Size: 6121 HpaI GTT'AAC Cuts at: 2824 3711 2824 Size: 887 5234 KpnI G_GTAC'C Cuts at: 3446 3446 Size: 6121 MfeI C'AATT_G Cuts at: 3720 3720 Size: 6121 MluI A'CGCG_T Cuts at: 5997 5997 Size: 6121 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5235 5419 1305 Size: 3930 184 2007 Fragments arranged by size: 3930 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2048 3251 10 Size: 54 1425 343 216 1203 2880 Fragments arranged by size: 2880 1425 1203 343 216 54 MslI CAynn'nnrTG Cuts at: 518 1166 2168 2690 3251 4079 4438 4597 518 Size: 648 1002 522 561 828 359 159 2042 Fragments arranged by size: 2042 1002 828 648 561 522 359 159 MspA1I CmG'CkG Cuts at: 1606 2153 2203 3537 4214 5362 5607 1606 Size: 547 50 1334 677 1148 245 2120 Fragments arranged by size: 2120 1334 1148 677 547 245 50 NcoI C'CATG_G Cuts at: 513 1065 2748 3246 513 Size: 552 1683 498 3388 Fragments arranged by size: 3388 1683 552 498 NdeI CA'TA_TG Cuts at: 387 387 Size: 6121 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6121 NotI GC'GGCC_GC Cuts at: 3568 3568 Size: 6121 NspI r_CATG'y Cuts at: 1132 1229 2018 2900 3479 5024 1132 Size: 97 789 882 579 1545 2229 Fragments arranged by size: 2229 1545 882 789 579 97 PasI CC'CwG_GG Cuts at: 1348 2287 2644 2761 2791 3179 1348 Size: 939 357 117 30 388 4290 Fragments arranged by size: 4290 939 388 357 117 30 PciI A'CATG_T Cuts at: 5020 5020 Size: 6121 PflMI CCAn_nnn'nTGG Cuts at: 1108 2440 1108 Size: 1332 4789 PfoI T'CCnGG_A Cuts at: 2844 2844 Size: 6121 PmeI GTTT'AAAC Cuts at: 3425 3425 Size: 6121 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4190 4222 5703 5735 4190 Size: 32 1481 32 4576 Fragments arranged by size: 4576 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2422 2593 2422 Size: 171 5950 PshAI GACnn'nnGTC Cuts at: 1613 2699 3380 1613 Size: 1086 681 4354 Fragments arranged by size: 4354 1086 681 PsiI TTA'TAA Cuts at: 3691 3691 Size: 6121 PspOMI G'GGCC_C Cuts at: 2549 2549 Size: 6121 PstI C_TGCA'G Cuts at: 830 2604 3048 3162 3222 3372 3473 830 Size: 1774 444 114 60 150 101 3478 Fragments arranged by size: 3478 1774 444 150 114 101 60 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6121 PvuII CAG'CTG Cuts at: 2153 2203 2153 Size: 50 6071 SalI G'TCGA_C Cuts at: 1936 3359 3463 1936 Size: 1423 104 4594 Fragments arranged by size: 4594 1423 104 SapI GCTCTTCn'nnn_ Cuts at: 2930 2930 Size: 6121 SbfI CC_TGCA'GG Cuts at: 3473 3473 Size: 6121 ScaI AGT'ACT Cuts at: 4369 6081 4369 Size: 1712 4409 SfcI C'TryA_G Cuts at: 826 1045 2600 3044 3158 3218 3368 3469 4604 Size: 219 1555 444 114 60 150 101 1135 Cuts at: 4604 5285 5476 826 Size: 681 191 1471 Fragments arranged by size: 1555 1471 1135 681 444 219 191 150 114 101 60 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6121 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6121 SmaI CCC'GGG Cuts at: 1872 3448 1872 Size: 1576 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4225 5126 5388 820 Size: 197 926 2282 901 262 1553 Fragments arranged by size: 2282 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3912 493 Size: 3419 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6121 SphI G_CATG'C Cuts at: 1132 2900 3479 1132 Size: 1768 579 3774 Fragments arranged by size: 3774 1768 579 SspI AAT'ATT Cuts at: 5 52 4045 5 Size: 47 3993 2081 Fragments arranged by size: 3993 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6121 StyI C'CwwG_G Cuts at: 513 1065 2118 2748 3246 3560 513 Size: 552 1053 630 498 314 3074 Fragments arranged by size: 3074 1053 630 552 498 314 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2300 2792 3134 4148 4165 4318 4503 Size: 1255 720 492 342 1014 17 153 185 Cuts at: 4503 4948 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1014 720 492 445 342 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4367 6079 96 Size: 274 80 33 51 1443 2390 1712 138 Fragments arranged by size: 2390 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2580 2612 3395 3427 6081 6113 2580 Size: 32 783 32 2654 32 2588 Fragments arranged by size: 2654 2588 783 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6121 XbaI T'CTAG_A Cuts at: 3457 3457 Size: 6121 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2320 2320 Size: 6121 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6121 XmaI C'CCGG_G Cuts at: 1870 3446 1870 Size: 1576 4545 XmnI GAAnn'nnTTC Cuts at: 4250 4250 Size: 6121 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3929 276 Size: 53 83 186 936 2395 2468 Fragments arranged by size: 2468 2395 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI