Restriction map for
pFN21AB8291
(Linear) (Six-base) MAPSORT of: KIBB8291.seq Check: 3465 from: 1 to: 1416
KIBB8291.orf
With 182 enzymes: SgfI *
March 3, 2008 11:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 440 499 1416
Size: 440 59 917
Fragments arranged by size:
917 440 59
AccI GT'mk_AC
Cuts at: 0 221 1355 1416
Size: 221 1134 61
Fragments arranged by size:
1134 221 61
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 354 1416
Size: 354 1062
AfeI AGC'GCT
Cuts at: 0 665 1416
Size: 665 751
AgeI A'CCGG_T
Cuts at: 0 735 1416
Size: 735 681
AleI CACnn'nnGTG
Cuts at: 0 163 1416
Size: 163 1253
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 181 1184 1416
Size: 181 1003 232
Fragments arranged by size:
1003 232 181
AlwNI CAG_nnn'CTG
Cuts at: 0 151 420 1072 1221 1416
Size: 151 269 652 149 195
Fragments arranged by size:
652 269 195 151 149
ApaI G_GGCC'C
Cuts at: 0 548 1416
Size: 548 868
ApoI r'AATT_y
Cuts at: 0 600 1416
Size: 600 816
BanI G'GyrC_C
Cuts at: 0 227 1168 1416
Size: 227 941 248
Fragments arranged by size:
941 248 227
BanII G_rGCy'C
Cuts at: 0 289 548 1416
Size: 289 259 868
Fragments arranged by size:
868 289 259
BbvCI CC'TCA_GC
Cuts at: 0 144 660 1416
Size: 144 516 756
Fragments arranged by size:
756 516 144
BglI GCCn_nnn'nGGC
Cuts at: 0 1243 1416
Size: 1243 173
Bme1580I G_kGCm'C
Cuts at: 0 170 177 548 1173 1416
Size: 170 7 371 625 243
Fragments arranged by size:
625 371 243 170 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 164 1416
Size: 164 1252
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 457 463 652 1363 1416
Size: 220 237 6 189 711 53
Fragments arranged by size:
711 237 220 189 53 6
Bpu10I CC'TnA_GC
Cuts at: 0 144 526 660 1416
Size: 144 382 134 756
Fragments arranged by size:
756 382 144 134
BsaWI w'CCGG_w
Cuts at: 0 619 735 1416
Size: 619 116 681
Fragments arranged by size:
681 619 116
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 550 575 580 605 817 847 1390 1416
Size: 550 25 5 25 212 30 543 26
Fragments arranged by size:
550 543 212 30 26 25 25 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 890 1339 1342 1345 1348 1351 1360 1363
Size: 890 449 3 3 3 3 9 3
Cuts at: 1363 1416
Size: 53
Fragments arranged by size:
890 449 53 9 3 3 3 3
3
BseYI C'CCAG_C
Cuts at: 0 18 101 194 654 1416
Size: 18 83 93 460 762
Fragments arranged by size:
762 460 93 83 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1253 1416
Size: 1253 163
BsiHKAI G_wGCw'C
Cuts at: 0 359 1416
Size: 359 1057
Bsp1286I G_dGCh'C
Cuts at: 0 170 177 289 359 548 1173 1416
Size: 170 7 112 70 189 625 243
Fragments arranged by size:
625 243 189 170 112 70 7
BspEI T'CCGG_A
Cuts at: 0 619 1416
Size: 619 797
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 440 499 530 1220 1416
Size: 440 59 31 690 196
Fragments arranged by size:
690 440 196 59 31
BsrBI CCG'CTC
Cuts at: 0 28 243 1416
Size: 28 215 1173
Fragments arranged by size:
1173 215 28
BsrFI r'CCGG_y
Cuts at: 0 735 1106 1416
Size: 735 371 310
Fragments arranged by size:
735 371 310
BstAPI GCAn_nnn'nTGC
Cuts at: 0 933 1047 1416
Size: 933 114 369
Fragments arranged by size:
933 369 114
BstEII G'GTnAC_C
Cuts at: 0 738 747 768 1416
Size: 738 9 21 648
Fragments arranged by size:
738 648 21 9
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1203 1416
Size: 1203 213
BstYI r'GATC_y
Cuts at: 0 800 1068 1416
Size: 800 268 348
Fragments arranged by size:
800 348 268
BstZ17I GTA'TAC
Cuts at: 0 222 1416
Size: 222 1194
BtgI C'CryG_G
Cuts at: 0 743 947 1241 1416
Size: 743 204 294 175
Fragments arranged by size:
743 294 204 175
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 389 666 1122 1416
Size: 389 277 456 294
Fragments arranged by size:
456 389 294 277
EaeI y'GGCC_r
Cuts at: 0 41 1244 1416
Size: 41 1203 172
Fragments arranged by size:
1203 172 41
EarI CTCTTCn'nnn_
Cuts at: 0 925 955 1295 1316 1416
Size: 925 30 340 21 100
Fragments arranged by size:
925 340 100 30 21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 294 1416
Size: 294 1122
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 220 354 457 463 652 1363 1416
Size: 220 134 103 6 189 711 53
Fragments arranged by size:
711 220 189 134 103 53 6
EcoO109I rG'GnC_Cy
Cuts at: 0 417 544 545 588 1416
Size: 417 127 1 43 828
Fragments arranged by size:
828 417 127 43 1
EcoRI G'AATT_C
Cuts at: 0 600 1416
Size: 600 816
HaeII r_GCGC'y
Cuts at: 0 667 1416
Size: 667 749
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 817 844 849 876 1083 1115 1416
Size: 817 27 5 27 207 32 301
Fragments arranged by size:
817 301 207 32 27 27 5
HincII GTy'rAC
Cuts at: 0 699 819 1356 1416
Size: 699 120 537 60
Fragments arranged by size:
699 537 120 60
HpaI GTT'AAC
Cuts at: 0 819 1416
Size: 819 597
MscI TGG'CCA
Cuts at: 0 43 1246 1416
Size: 43 1203 170
Fragments arranged by size:
1203 170 43
MslI CAynn'nnrTG
Cuts at: 0 163 685 1246 1416
Size: 163 522 561 170
Fragments arranged by size:
561 522 170 163
MspA1I CmG'CkG
Cuts at: 0 148 198 1416
Size: 148 50 1218
Fragments arranged by size:
1218 148 50
NcoI C'CATG_G
Cuts at: 0 743 1241 1416
Size: 743 498 175
Fragments arranged by size:
743 498 175
NspI r_CATG'y
Cuts at: 0 13 895 1416
Size: 13 882 521
Fragments arranged by size:
882 521 13
PasI CC'CwG_GG
Cuts at: 0 282 639 756 786 1174 1416
Size: 282 357 117 30 388 242
Fragments arranged by size:
388 357 282 242 117 30
PflMI CCAn_nnn'nTGG
Cuts at: 0 435 1416
Size: 435 981
PfoI T'CCnGG_A
Cuts at: 0 839 1416
Size: 839 577
PpuMI rG'GwC_Cy
Cuts at: 0 417 588 1416
Size: 417 171 828
Fragments arranged by size:
828 417 171
PshAI GACnn'nnGTC
Cuts at: 0 694 1375 1416
Size: 694 681 41
Fragments arranged by size:
694 681 41
PspOMI G'GGCC_C
Cuts at: 0 544 1416
Size: 544 872
PstI C_TGCA'G
Cuts at: 0 599 1043 1157 1217 1367 1416
Size: 599 444 114 60 150 49
Fragments arranged by size:
599 444 150 114 60 49
PvuII CAG'CTG
Cuts at: 0 148 198 1416
Size: 148 50 1218
Fragments arranged by size:
1218 148 50
SalI G'TCGA_C
Cuts at: 0 1354 1416
Size: 1354 62
SapI GCTCTTCn'nnn_
Cuts at: 0 925 1416
Size: 925 491
SfcI C'TryA_G
Cuts at: 0 595 1039 1153 1213 1363 1416
Size: 595 444 114 60 150 53
Fragments arranged by size:
595 444 150 114 60 53
SphI G_CATG'C
Cuts at: 0 895 1416
Size: 895 521
StyI C'CwwG_G
Cuts at: 0 113 743 1241 1416
Size: 113 630 498 175
Fragments arranged by size:
630 498 175 113
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 295 787 1129 1416
Size: 295 492 342 287
Fragments arranged by size:
492 342 295 287
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 575 607 1390 1416
Size: 575 32 783 26
Fragments arranged by size:
783 575 32 26
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 315 1416
Size: 315 1101
Enzymes that do cut:
AarI AccI AcuI AfeI AgeI AleI AlfI AlwNI
ApaI ApoI BanI BanII BbvCI BglI Bme1580I BmrI
BpmI Bpu10I BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
Bsp1286I BspEI BspMI BsrBI BsrFI BstAPI BstEII BstXI
BstYI BstZ17I BtgI BtgZI EaeI EarI EciI Eco57MI
EcoO109I EcoRI HaeII Hin4I HincII HpaI MscI MslI
MspA1I NcoI NspI PasI PflMI PfoI PpuMI PshAI
PspOMI PstI PvuII SalI SapI SfcI SphI StyI
TaqII TstI XcmI
Enzymes that do not cut:
AatII Acc65I AclI AflII AflIII AhdI AloI ApaLI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BbeI
BbsI BcgI BciVI BclI BfrBI BglII BlpI BmgBI
BmtI BplI BpuEI BsaI BsaAI BsaBI BsaHI BsiEI
BsiWI BsmI BsmBI BspHI BsrDI BsrGI BssHII BssSI
BstBI Bsu36I BtsI ClaI CspCI DraI DraIII DrdI
EagI EcoICRI EcoNI EcoRV FalI FseI FspI FspAI
HindIII KasI KpnI MfeI MluI MmeI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PciI
PmeI PmlI PpiI PsiI PspXI PsrI PvuI RsrII
SacI SacII SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SrfI SspI
StuI SwaI TatI Tth111I XbaI XhoI XmaI XmnI
ZraI