(Circular) (Six-base) MAPSORT of: pFN21AB8285.seq Check: 6180 from: 1 to: 5887 pFN21AB8285 5887 bp With 182 enzymes: SgfI * March 28, 2012 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3697 278 Size: 53 83 186 936 2161 2468 Fragments arranged by size: 2468 2161 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3230 1937 Size: 1293 4594 Acc65I G'GTAC_C Cuts at: 3208 3208 Size: 5887 AclI AA'CG_TT Cuts at: 1563 4014 4387 1563 Size: 2451 373 3063 Fragments arranged by size: 3063 2451 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2475 2785 3950 5334 5602 1632 Size: 843 310 1165 1384 268 1917 Fragments arranged by size: 1917 1384 1165 843 310 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5690 AflIII A'CryG_T Cuts at: 1203 4786 5763 1203 Size: 3583 977 1327 Fragments arranged by size: 3583 1327 977 AgeI A'CCGG_T Cuts at: 4718 4718 Size: 5887 AhdI GACnn_n'nnGTC Cuts at: 4616 4616 Size: 5887 AleI CACnn'nnGTG Cuts at: 1166 2378 2448 2498 1166 Size: 1212 70 50 4555 Fragments arranged by size: 4555 1212 70 50 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5887 AlwNI CAG_nnn'CTG Cuts at: 3630 5202 3630 Size: 1572 4315 ApaLI G'TGCA_C Cuts at: 3944 5100 3944 Size: 1156 4731 ApoI r'AATT_y Cuts at: 1493 2654 2945 2963 3196 3422 3574 5683 1493 Size: 1161 291 18 233 226 152 2109 1697 Fragments arranged by size: 2109 1697 1161 291 233 226 152 18 AseI AT'TA_AT Cuts at: 160 1032 4441 160 Size: 872 3409 1606 Fragments arranged by size: 3409 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5887 AvaI C'yCGr_G Cuts at: 1870 1943 3212 1870 Size: 73 1269 4545 Fragments arranged by size: 4545 1269 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2087 2120 2087 Size: 33 5854 BamHI G'GATC_C Cuts at: 3217 3584 3217 Size: 367 5520 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3208 4663 618 Size: 325 217 627 1421 1455 1842 Fragments arranged by size: 1842 1455 1421 627 325 217 BanII G_rGCy'C Cuts at: 1391 3206 1391 Size: 1815 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4916 BbvCI CC'TCA_GC Cuts at: 2043 2043 Size: 5887 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4078 4112 1903 Size: 34 2141 34 3678 Fragments arranged by size: 3678 2141 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3779 4989 3779 Size: 1210 4677 BclI T'GATC_A Cuts at: 1548 1548 Size: 5887 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4498 136 Size: 107 122 71 631 605 2826 1525 Fragments arranged by size: 2826 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5882 5882 Size: 5887 BlpI GC'TnA_GC Cuts at: 3304 3304 Size: 5887 Bme1580I G_kGCm'C Cuts at: 1790 3948 5104 1790 Size: 2158 1156 2573 Fragments arranged by size: 2573 2158 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5887 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2950 3618 4576 Size: 416 53 83 399 24 1533 668 958 Cuts at: 4576 442 Size: 1753 Fragments arranged by size: 1753 1533 958 668 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5887 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3062 4547 1239 Size: 1823 1485 2579 Fragments arranged by size: 2579 1823 1485 Bpu10I CC'TnA_GC Cuts at: 2043 2125 2394 2698 2043 Size: 82 269 304 5232 Fragments arranged by size: 5232 304 269 82 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2288 4012 4877 5175 2288 Size: 1724 865 298 3000 Fragments arranged by size: 3000 1724 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2745 4550 882 Size: 617 1246 1805 2219 Fragments arranged by size: 2219 1805 1246 617 BsaAI yAC'GTr Cuts at: 493 1204 2792 3678 493 Size: 711 1588 886 2702 Fragments arranged by size: 2702 1588 886 711 BsaBI GATnn'nnATC Cuts at: 5881 5881 Size: 5887 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3694 4076 4711 275 Size: 53 83 186 936 2161 382 635 1451 Fragments arranged by size: 2161 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4320 4718 4992 5139 5550 4320 Size: 398 274 147 411 4657 Fragments arranged by size: 4657 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5662 Fragments arranged by size: 5662 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3065 1186 Size: 1879 4008 BseYI C'CCAG_C Cuts at: 3179 5090 5586 3179 Size: 1911 496 3480 Fragments arranged by size: 3480 1911 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2524 1163 Size: 573 788 4526 Fragments arranged by size: 4526 788 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3337 4098 5126 1540 Size: 275 186 1336 761 1028 2301 Fragments arranged by size: 2301 1336 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3948 4033 5104 3948 Size: 85 1071 4731 Fragments arranged by size: 4731 1071 85 BsmI GAATG_Cn' Cuts at: 3148 3398 3491 3148 Size: 250 93 5544 Fragments arranged by size: 5544 250 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3206 3948 4033 5104 1391 Size: 399 1416 742 85 1071 2174 Fragments arranged by size: 2174 1416 1071 742 399 85 BspEI T'CCGG_A Cuts at: 5550 5550 Size: 5887 BspHI T'CATG_A Cuts at: 3776 5506 3776 Size: 1730 4157 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3242 844 Size: 2398 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3774 1394 Size: 429 1951 3507 Fragments arranged by size: 3507 1951 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4382 4556 65 Size: 1183 3134 174 1396 Fragments arranged by size: 3134 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4531 4718 1700 Size: 110 2721 187 2869 Fragments arranged by size: 2869 2721 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5887 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5887 BssSI C'ACGA_G Cuts at: 3947 4959 3947 Size: 1012 4875 BstAPI GCAn_nnn'nTGC Cuts at: 794 3143 794 Size: 2349 3538 BstBI TT'CG_AA Cuts at: 4691 4691 Size: 5887 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5887 BstXI CCAn_nnnn'nTGG Cuts at: 2173 2348 2173 Size: 175 5712 BstYI r'GATC_y Cuts at: 1972 3217 3584 3969 3986 5438 5524 5536 5882 Size: 1245 367 385 17 1452 86 12 346 Cuts at: 5882 1972 Size: 1977 Fragments arranged by size: 1977 1452 1245 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5335 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2068 2341 487 Size: 683 56 842 273 4033 Fragments arranged by size: 4033 842 683 273 56 BtsI GCAGTG_nn' Cuts at: 3403 4197 4217 3403 Size: 794 20 5073 Fragments arranged by size: 5073 794 20 ClaI AT'CG_AT Cuts at: 1554 2589 1554 Size: 1035 4852 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5852 DraI TTT'AAA Cuts at: 2962 3191 2962 Size: 229 5658 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2336 4894 809 Size: 507 1020 2558 1802 Fragments arranged by size: 2558 1802 1020 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3334 4223 5852 Size: 54 1425 50 275 18 1504 889 1629 Cuts at: 5852 8 Size: 43 Fragments arranged by size: 1629 1504 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3334 1537 Size: 275 1522 4090 Fragments arranged by size: 4090 1522 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2276 3817 1353 Size: 923 1541 3423 Fragments arranged by size: 3423 1541 923 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4474 4848 4994 387 Size: 1064 138 2885 374 146 1280 Fragments arranged by size: 2885 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2475 2785 3062 3950 4547 5334 5602 Size: 393 843 310 277 888 597 787 268 Cuts at: 5602 1239 Size: 1524 Fragments arranged by size: 1524 888 843 787 597 393 310 277 268 EcoO109I rG'GnC_Cy Cuts at: 2047 2400 3070 2047 Size: 353 670 4864 Fragments arranged by size: 4864 670 353 EcoRI G'AATT_C Cuts at: 3196 3574 3196 Size: 378 5509 FspI TGC'GCA Cuts at: 4393 4393 Size: 5887 HaeII r_GCGC'y Cuts at: 1678 5034 1678 Size: 3356 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4528 4560 4602 4634 1373 Size: 32 135 32 2956 32 42 32 2626 Fragments arranged by size: 2956 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3231 3477 669 Size: 988 281 1293 246 3079 Fragments arranged by size: 3079 1293 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5887 HpaI GTT'AAC Cuts at: 3477 3477 Size: 5887 KpnI G_GTAC'C Cuts at: 3212 3212 Size: 5887 MfeI C'AATT_G Cuts at: 3486 3486 Size: 5887 MluI A'CGCG_T Cuts at: 5763 5763 Size: 5887 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5001 5185 1305 Size: 3696 184 2007 Fragments arranged by size: 3696 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4065 Fragments arranged by size: 4065 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2171 2346 2378 2448 2498 3845 4204 Size: 648 1005 175 32 70 50 1347 359 Cuts at: 4204 4363 518 Size: 159 2042 Fragments arranged by size: 2042 1347 1005 648 359 175 159 70 50 32 MspA1I CmG'CkG Cuts at: 1606 3303 3980 5128 5373 1606 Size: 1697 677 1148 245 2120 Fragments arranged by size: 2120 1697 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5335 NdeI CA'TA_TG Cuts at: 387 387 Size: 5887 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5887 NotI GC'GGCC_GC Cuts at: 3334 3334 Size: 5887 NspI r_CATG'y Cuts at: 1132 1229 2585 3245 4790 1132 Size: 97 1356 660 1545 2229 Fragments arranged by size: 2229 1545 1356 660 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5887 PciI A'CATG_T Cuts at: 4786 4786 Size: 5887 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5887 PfoI T'CCnGG_A Cuts at: 3051 3051 Size: 5887 PmeI GTTT'AAAC Cuts at: 3191 3191 Size: 5887 PmlI CAC'GTG Cuts at: 2792 2792 Size: 5887 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3956 3988 5469 5501 3956 Size: 32 1481 32 4342 Fragments arranged by size: 4342 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2047 2400 2047 Size: 353 5534 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5887 PsiI TTA'TAA Cuts at: 3457 3457 Size: 5887 PstI C_TGCA'G Cuts at: 830 3139 3239 830 Size: 2309 100 3478 Fragments arranged by size: 3478 2309 100 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5887 SalI G'TCGA_C Cuts at: 1936 3229 1936 Size: 1293 4594 SanDI GG'GwC_CC Cuts at: 2400 2400 Size: 5887 SapI GCTCTTCn'nnn_ Cuts at: 2276 2276 Size: 5887 SbfI CC_TGCA'GG Cuts at: 3239 3239 Size: 5887 ScaI AGT'ACT Cuts at: 4135 5847 4135 Size: 1712 4175 SexAI A'CCwGG_T Cuts at: 2049 2049 Size: 5887 SfcI C'TryA_G Cuts at: 826 1045 3135 3235 4370 5051 5242 826 Size: 219 2090 100 1135 681 191 1471 Fragments arranged by size: 2090 1471 1135 681 219 191 100 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5887 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5887 SmaI CCC'GGG Cuts at: 1872 3214 1872 Size: 1342 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2303 3991 4892 5154 820 Size: 197 926 360 1688 901 262 1553 Fragments arranged by size: 1688 1553 926 901 360 262 197 SnaBI TAC'GTA Cuts at: 493 3678 493 Size: 3185 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5887 SphI G_CATG'C Cuts at: 1132 3245 1132 Size: 2113 3774 SspI AAT'ATT Cuts at: 5 52 3811 5 Size: 47 3759 2081 Fragments arranged by size: 3759 2081 47 StuI AGG'CCT Cuts at: 1517 3125 1517 Size: 1608 4279 StyI C'CwwG_G Cuts at: 513 1065 2106 2332 2715 3326 513 Size: 552 1041 226 383 611 3074 Fragments arranged by size: 3074 1041 611 552 383 226 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2091 2867 3914 3931 4084 4269 4714 Size: 1255 511 776 1047 17 153 185 445 Cuts at: 4714 325 Size: 1498 Fragments arranged by size: 1498 1255 1047 776 511 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4133 5845 96 Size: 274 80 33 51 1443 2156 1712 138 Fragments arranged by size: 2156 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2992 3024 5847 5879 2992 Size: 32 2823 32 3000 Fragments arranged by size: 3000 2823 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2051 1316 Size: 735 5152 XbaI T'CTAG_A Cuts at: 3223 3223 Size: 5887 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2068 2068 Size: 5887 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5887 XmaI C'CCGG_G Cuts at: 1870 3212 1870 Size: 1342 4545 XmnI GAAnn'nnTTC Cuts at: 2192 4016 2192 Size: 1824 4063 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3695 276 Size: 53 83 186 936 2161 2468 Fragments arranged by size: 2468 2161 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI PvuII RsrII SacI SacII SfiI SfoI SrfI SwaI