(Circular) (Six-base) MAPSORT of: pFN21AB8284.seq Check: 3806 from: 1 to: 5941 pFN21AB8284 5941 bp With 182 enzymes: SgfI * February 27, 2009 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3751 278 Size: 53 83 186 936 2215 2468 Fragments arranged by size: 2468 2215 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3177 3284 1937 Size: 1240 107 4594 Fragments arranged by size: 4594 1240 107 Acc65I G'GTAC_C Cuts at: 2561 3262 2561 Size: 701 5240 AclI AA'CG_TT Cuts at: 1563 4068 4441 1563 Size: 2505 373 3063 Fragments arranged by size: 3063 2505 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2249 2450 2522 2979 3224 4004 5388 5656 Size: 617 201 72 457 245 780 1384 268 Cuts at: 5656 1632 Size: 1917 Fragments arranged by size: 1917 1384 780 617 457 268 245 201 72 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5744 AflIII A'CryG_T Cuts at: 1203 2100 2447 4840 5817 1203 Size: 897 347 2393 977 1327 Fragments arranged by size: 2393 1327 977 897 347 AgeI A'CCGG_T Cuts at: 4772 4772 Size: 5941 AhdI GACnn_n'nnGTC Cuts at: 2503 4670 2503 Size: 2167 3774 AleI CACnn'nnGTG Cuts at: 1166 2694 3044 1166 Size: 1528 350 4063 Fragments arranged by size: 4063 1528 350 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5941 AlwNI CAG_nnn'CTG Cuts at: 2473 3002 3684 5256 2473 Size: 529 682 1572 3158 Fragments arranged by size: 3158 1572 682 529 ApaI G_GGCC'C Cuts at: 2945 2945 Size: 5941 ApaLI G'TGCA_C Cuts at: 3998 5154 3998 Size: 1156 4785 ApoI r'AATT_y Cuts at: 1493 3250 3476 3628 5737 1493 Size: 1757 226 152 2109 1697 Fragments arranged by size: 2109 1757 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4495 160 Size: 872 3463 1606 Fragments arranged by size: 3463 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5941 AvaI C'yCGr_G Cuts at: 1870 1943 2454 2493 2692 3266 1870 Size: 73 511 39 199 574 4545 Fragments arranged by size: 4545 574 511 199 73 39 BamHI G'GATC_C Cuts at: 2013 3271 3638 2013 Size: 1258 367 4316 Fragments arranged by size: 4316 1258 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2561 3262 4717 618 Size: 325 217 627 774 701 1455 1842 Fragments arranged by size: 1842 1455 774 701 627 325 217 BanII G_rGCy'C Cuts at: 1391 2945 3260 1391 Size: 1554 315 4072 Fragments arranged by size: 4072 1554 315 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2499 928 Size: 971 600 4370 Fragments arranged by size: 4370 971 600 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4132 4166 1903 Size: 34 2195 34 3678 Fragments arranged by size: 3678 2195 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3045 3833 5043 3045 Size: 788 1210 3943 Fragments arranged by size: 3943 1210 788 BclI T'GATC_A Cuts at: 1548 2338 2922 1548 Size: 790 584 4567 Fragments arranged by size: 4567 790 584 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2176 4552 136 Size: 107 122 71 631 605 504 2376 1525 Fragments arranged by size: 2376 1525 631 605 504 122 107 71 BglII A'GATC_T Cuts at: 3114 5936 3114 Size: 2822 3119 BlpI GC'TnA_GC Cuts at: 3358 3358 Size: 5941 Bme1580I G_kGCm'C Cuts at: 1790 2455 2945 4002 5158 1790 Size: 665 490 1057 1156 2573 Fragments arranged by size: 2573 1156 1057 665 490 BmgBI CAC'GTC Cuts at: 1318 2198 1318 Size: 880 5061 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3672 4630 442 Size: 416 53 83 399 24 2255 958 1753 Fragments arranged by size: 2255 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5941 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2084 2551 3041 4601 1239 Size: 845 467 490 1560 2579 Fragments arranged by size: 2579 1560 845 490 467 Bpu10I CC'TnA_GC Cuts at: 2434 2434 Size: 5941 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4066 4931 5229 4066 Size: 865 298 4778 Fragments arranged by size: 4778 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2067 2150 2545 4604 882 Size: 617 568 83 395 2059 2219 Fragments arranged by size: 2219 2059 617 568 395 83 BsaAI yAC'GTr Cuts at: 493 1204 3732 493 Size: 711 2528 2702 Fragments arranged by size: 2702 2528 711 BsaBI GATnn'nnATC Cuts at: 5935 5935 Size: 5941 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3748 4130 4765 275 Size: 53 83 186 936 2215 382 635 1451 Fragments arranged by size: 2215 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2202 2874 4374 4772 5046 5193 5604 2202 Size: 672 1500 398 274 147 411 2539 Fragments arranged by size: 2539 1500 672 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5716 Fragments arranged by size: 5716 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2757 1186 Size: 1571 4370 BseYI C'CCAG_C Cuts at: 2018 2223 2584 2618 5144 5640 2018 Size: 205 361 34 2526 496 2319 Fragments arranged by size: 2526 2319 496 361 205 34 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5368 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3391 4152 5180 1540 Size: 275 186 1390 761 1028 2301 Fragments arranged by size: 2301 1390 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2070 4002 4087 5158 2070 Size: 1932 85 1071 2853 Fragments arranged by size: 2853 1932 1071 85 BsmI GAATG_Cn' Cuts at: 3452 3545 3452 Size: 93 5848 BsmBI CGTCTCn'nnnn_ Cuts at: 2188 2188 Size: 5941 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2070 2455 2945 3260 4002 4087 5158 Size: 399 280 385 490 315 742 85 1071 Cuts at: 5158 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 490 399 385 315 280 85 BspEI T'CCGG_A Cuts at: 5604 5604 Size: 5941 BspHI T'CATG_A Cuts at: 3099 3830 5560 3099 Size: 731 1730 3480 Fragments arranged by size: 3480 1730 731 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2796 3018 3296 844 Size: 1952 222 278 3489 Fragments arranged by size: 3489 1952 278 222 BsrBI CCG'CTC Cuts at: 1394 1823 2011 2709 3828 1394 Size: 429 188 698 1119 3507 Fragments arranged by size: 3507 1119 698 429 188 BsrDI GCAATG_nn' Cuts at: 65 1248 2358 4436 4610 65 Size: 1183 1110 2078 174 1396 Fragments arranged by size: 2078 1396 1183 1110 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2175 4585 4772 1700 Size: 110 365 2410 187 2869 Fragments arranged by size: 2869 2410 365 187 110 BsrGI T'GTAC_A Cuts at: 96 2112 96 Size: 2016 3925 BssHII G'CGCG_C Cuts at: 1925 2022 1925 Size: 97 5844 BssSI C'ACGA_G Cuts at: 2483 4001 5013 2483 Size: 1518 1012 3411 Fragments arranged by size: 3411 1518 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5941 BstBI TT'CG_AA Cuts at: 4745 4745 Size: 5941 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5941 BstXI CCAn_nnnn'nTGG Cuts at: 2599 2599 Size: 5941 BstYI r'GATC_y Cuts at: 1972 2013 2233 3114 3271 3638 4023 4040 5492 Size: 41 220 881 157 367 385 17 1452 Cuts at: 5492 5578 5590 5936 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 881 385 367 346 220 157 86 41 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2797 513 Size: 552 939 793 3657 Fragments arranged by size: 3657 939 793 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2203 487 Size: 683 56 977 4225 Fragments arranged by size: 4225 977 683 56 BtsI GCAGTG_nn' Cuts at: 2292 2776 2920 3457 4251 4271 2292 Size: 484 144 537 794 20 3962 Fragments arranged by size: 3962 794 537 484 144 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5941 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5906 DraI TTT'AAA Cuts at: 3222 3245 3222 Size: 23 5918 DraIII CAC_nnn'GTG Cuts at: 2536 2536 Size: 5941 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3185 4948 809 Size: 507 1869 1763 1802 Fragments arranged by size: 1869 1802 1763 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2475 2628 3388 Size: 54 1425 50 275 18 645 153 760 Cuts at: 3388 4277 5906 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 760 645 275 153 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3388 1537 Size: 275 1576 4090 Fragments arranged by size: 4090 1576 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3187 3871 1353 Size: 1834 684 3423 Fragments arranged by size: 3423 1834 684 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4528 4902 5048 387 Size: 1064 138 2939 374 146 1280 Fragments arranged by size: 2939 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2084 2249 2450 2522 2551 2979 3041 Size: 393 452 165 201 72 29 428 62 Cuts at: 3041 3224 4004 4601 5388 5656 1239 Size: 183 780 597 787 268 1524 Fragments arranged by size: 1524 787 780 597 452 428 393 268 201 183 165 72 62 29 EcoO109I rG'GnC_Cy Cuts at: 2497 2645 2497 Size: 148 5793 EcoRI G'AATT_C Cuts at: 3250 3628 3250 Size: 378 5563 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2779 2779 Size: 5941 FspI TGC'GCA Cuts at: 2768 4447 2768 Size: 1679 4262 HaeII r_GCGC'y Cuts at: 1678 5088 1678 Size: 3410 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2489 2521 2671 2703 2811 Size: 32 135 32 917 32 150 32 108 Cuts at: 2811 2843 3101 3106 3133 3138 4582 4614 4656 Size: 32 258 5 27 5 1444 32 42 Cuts at: 4656 4688 1373 Size: 32 2626 Fragments arranged by size: 2626 1444 917 258 150 135 108 42 32 32 32 32 32 32 32 27 5 5 HincII GTy'rAC Cuts at: 669 1657 1938 3178 3285 3531 669 Size: 988 281 1240 107 246 3079 Fragments arranged by size: 3079 1240 988 281 246 107 HindIII A'AGCT_T Cuts at: 748 748 Size: 5941 HpaI GTT'AAC Cuts at: 3531 3531 Size: 5941 KpnI G_GTAC'C Cuts at: 2565 3266 2565 Size: 701 5240 MfeI C'AATT_G Cuts at: 3540 3540 Size: 5941 MluI A'CGCG_T Cuts at: 5817 5817 Size: 5941 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5055 5239 1305 Size: 3750 184 2007 Fragments arranged by size: 3750 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2477 10 Size: 54 1425 343 645 3474 Fragments arranged by size: 3474 1425 645 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2694 3044 3899 4258 4417 518 Size: 648 1528 350 855 359 159 2042 Fragments arranged by size: 2042 1528 855 648 359 350 159 MspA1I CmG'CkG Cuts at: 1606 2227 2677 3357 4034 5182 5427 1606 Size: 621 450 680 677 1148 245 2120 Fragments arranged by size: 2120 1148 680 677 621 450 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4450 Fragments arranged by size: 4450 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5941 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5941 NotI GC'GGCC_GC Cuts at: 3388 3388 Size: 5941 NspI r_CATG'y Cuts at: 1132 1229 2104 2315 2451 3299 4844 1132 Size: 97 875 211 136 848 1545 2229 Fragments arranged by size: 2229 1545 875 848 211 136 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5941 PciI A'CATG_T Cuts at: 2100 2447 4840 2100 Size: 347 2393 3201 Fragments arranged by size: 3201 2393 347 PflMI CCAn_nnn'nTGG Cuts at: 1108 2536 1108 Size: 1428 4513 PfoI T'CCnGG_A Cuts at: 2567 2567 Size: 5941 PmeI GTTT'AAAC Cuts at: 3245 3245 Size: 5941 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4010 4042 5523 5555 4010 Size: 32 1481 32 4396 Fragments arranged by size: 4396 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2497 2645 2497 Size: 148 5793 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5941 PsiI TTA'TAA Cuts at: 3511 3511 Size: 5941 PspOMI G'GGCC_C Cuts at: 2941 2941 Size: 5941 PspXI vC'TCGA_Gb Cuts at: 2692 2692 Size: 5941 PstI C_TGCA'G Cuts at: 830 2370 2556 3293 830 Size: 1540 186 737 3478 Fragments arranged by size: 3478 1540 737 186 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5941 PvuII CAG'CTG Cuts at: 2227 2227 Size: 5941 SalI G'TCGA_C Cuts at: 1936 3176 3283 1936 Size: 1240 107 4594 Fragments arranged by size: 4594 1240 107 SbfI CC_TGCA'GG Cuts at: 3293 3293 Size: 5941 ScaI AGT'ACT Cuts at: 4189 5901 4189 Size: 1712 4229 SfcI C'TryA_G Cuts at: 826 1045 2366 2552 3289 4424 5105 5296 826 Size: 219 1321 186 737 1135 681 191 1471 Fragments arranged by size: 1471 1321 1135 737 681 219 191 186 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5941 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5941 SmaI CCC'GGG Cuts at: 1872 2456 3268 1872 Size: 584 812 4545 Fragments arranged by size: 4545 812 584 SmlI C'TyrA_G Cuts at: 820 1017 1943 2692 4045 4946 5208 820 Size: 197 926 749 1353 901 262 1553 Fragments arranged by size: 1553 1353 926 901 749 262 197 SnaBI TAC'GTA Cuts at: 493 3732 493 Size: 3239 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5941 SphI G_CATG'C Cuts at: 1132 3299 1132 Size: 2167 3774 SspI AAT'ATT Cuts at: 5 52 3865 5 Size: 47 3813 2081 Fragments arranged by size: 3813 2081 47 StuI AGG'CCT Cuts at: 1517 3066 1517 Size: 1549 4392 StyI C'CwwG_G Cuts at: 513 1065 2004 3380 513 Size: 552 939 1376 3074 Fragments arranged by size: 3074 1376 939 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2537 2964 3968 3985 4138 4323 4768 Size: 1255 957 427 1004 17 153 185 445 Cuts at: 4768 325 Size: 1498 Fragments arranged by size: 1498 1255 1004 957 445 427 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2112 4187 5899 Size: 274 80 33 51 1443 135 2075 1712 Cuts at: 5899 96 Size: 138 Fragments arranged by size: 2075 1712 1443 274 138 135 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5901 5933 5901 Size: 32 5909 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5941 XbaI T'CTAG_A Cuts at: 3277 3277 Size: 5941 XhoI C'TCGA_G Cuts at: 1943 2692 1943 Size: 749 5192 XmaI C'CCGG_G Cuts at: 1870 2454 3266 1870 Size: 584 812 4545 Fragments arranged by size: 4545 812 584 XmnI GAAnn'nnTTC Cuts at: 4070 4070 Size: 5941 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3749 276 Size: 53 83 186 936 2215 2468 Fragments arranged by size: 2468 2215 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI