Restriction map for
pFN21AB8284
(Linear) (Six-base) MAPSORT of: KIBB8284.seq Check: 6756 from: 1 to: 1236
KIBB8284.orf
With 182 enzymes: SgfI *
March 3, 2008 11:04 ..
AccI GT'mk_AC
Cuts at: 0 1172 1236
Size: 1172 64
Acc65I G'GTAC_C
Cuts at: 0 556 1236
Size: 556 680
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 244 445 517 974 1219 1236
Size: 244 201 72 457 245 17
Fragments arranged by size:
457 245 244 201 72 17
AflIII A'CryG_T
Cuts at: 0 95 442 1236
Size: 95 347 794
Fragments arranged by size:
794 347 95
AhdI GACnn_n'nnGTC
Cuts at: 0 498 1236
Size: 498 738
AleI CACnn'nnGTG
Cuts at: 0 689 1039 1236
Size: 689 350 197
Fragments arranged by size:
689 350 197
AlwNI CAG_nnn'CTG
Cuts at: 0 468 997 1236
Size: 468 529 239
Fragments arranged by size:
529 468 239
ApaI G_GGCC'C
Cuts at: 0 940 1236
Size: 940 296
AvaI C'yCGr_G
Cuts at: 0 449 488 687 1236
Size: 449 39 199 549
Fragments arranged by size:
549 449 199 39
BamHI G'GATC_C
Cuts at: 0 8 1236
Size: 8 1228
BanI G'GyrC_C
Cuts at: 0 556 1236
Size: 556 680
BanII G_rGCy'C
Cuts at: 0 940 1236
Size: 940 296
BbsI GAAGACnn'nnnn_
Cuts at: 0 494 1236
Size: 494 742
BciVI GTATCCnnnnn_n'
Cuts at: 0 1040 1236
Size: 1040 196
BclI T'GATC_A
Cuts at: 0 333 917 1236
Size: 333 584 319
Fragments arranged by size:
584 333 319
BglI GCCn_nnn'nGGC
Cuts at: 0 171 1236
Size: 171 1065
BglII A'GATC_T
Cuts at: 0 1109 1236
Size: 1109 127
Bme1580I G_kGCm'C
Cuts at: 0 450 940 1236
Size: 450 490 296
Fragments arranged by size:
490 450 296
BmgBI CAC'GTC
Cuts at: 0 193 1236
Size: 193 1043
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 546 1036 1236
Size: 79 467 490 200
Fragments arranged by size:
490 467 200 79
Bpu10I CC'TnA_GC
Cuts at: 0 429 1236
Size: 429 807
BsaI GGTCTCn'nnnn_
Cuts at: 0 62 145 540 1236
Size: 62 83 395 696
Fragments arranged by size:
696 395 83 62
BsaWI w'CCGG_w
Cuts at: 0 197 869 1236
Size: 197 672 367
Fragments arranged by size:
672 367 197
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 752 1236
Size: 752 484
BseYI C'CCAG_C
Cuts at: 0 13 218 579 613 1236
Size: 13 205 361 34 623
Fragments arranged by size:
623 361 205 34 13
BsiHKAI G_wGCw'C
Cuts at: 0 65 1236
Size: 65 1171
BsmBI CGTCTCn'nnnn_
Cuts at: 0 183 1236
Size: 183 1053
Bsp1286I G_dGCh'C
Cuts at: 0 65 450 940 1236
Size: 65 385 490 296
Fragments arranged by size:
490 385 296 65
BspHI T'CATG_A
Cuts at: 0 1094 1236
Size: 1094 142
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 791 1013 1236
Size: 791 222 223
Fragments arranged by size:
791 223 222
BsrBI CCG'CTC
Cuts at: 0 6 704 1236
Size: 6 698 532
Fragments arranged by size:
698 532 6
BsrDI GCAATG_nn'
Cuts at: 0 353 1236
Size: 353 883
BsrFI r'CCGG_y
Cuts at: 0 170 1236
Size: 170 1066
BsrGI T'GTAC_A
Cuts at: 0 107 1236
Size: 107 1129
BssHII G'CGCG_C
Cuts at: 0 17 1236
Size: 17 1219
BssSI C'ACGA_G
Cuts at: 0 478 1236
Size: 478 758
BstXI CCAn_nnnn'nTGG
Cuts at: 0 594 1236
Size: 594 642
BstYI r'GATC_y
Cuts at: 0 8 228 1109 1236
Size: 8 220 881 127
Fragments arranged by size:
881 220 127 8
BtgI C'CryG_G
Cuts at: 0 792 1236
Size: 792 444
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 198 1236
Size: 198 1038
BtsI GCAGTG_nn'
Cuts at: 0 287 771 915 1236
Size: 287 484 144 321
Fragments arranged by size:
484 321 287 144
DraI TTT'AAA
Cuts at: 0 1217 1236
Size: 1217 19
DraIII CAC_nnn'GTG
Cuts at: 0 531 1236
Size: 531 705
DrdI GACnn_nn'nnGTC
Cuts at: 0 1180 1236
Size: 1180 56
EaeI y'GGCC_r
Cuts at: 0 470 623 1236
Size: 470 153 613
Fragments arranged by size:
613 470 153
EarI CTCTTCn'nnn_
Cuts at: 0 1182 1236
Size: 1182 54
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 79 244 445 517 546 974 1036 1219
Size: 79 165 201 72 29 428 62 183
Cuts at: 1219 1236
Size: 17
Fragments arranged by size:
428 201 183 165 79 72 62 29
17
EcoO109I rG'GnC_Cy
Cuts at: 0 492 640 1236
Size: 492 148 596
Fragments arranged by size:
596 492 148
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 774 1236
Size: 774 462
FspI TGC'GCA
Cuts at: 0 763 1236
Size: 763 473
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 484 516 666 698 806 838 1096 1101
Size: 484 32 150 32 108 32 258 5
Cuts at: 1101 1128 1133 1236
Size: 27 5 103
Fragments arranged by size:
484 258 150 108 103 32 32 32
27 5 5
HincII GTy'rAC
Cuts at: 0 1173 1236
Size: 1173 63
KpnI G_GTAC'C
Cuts at: 0 560 1236
Size: 560 676
MscI TGG'CCA
Cuts at: 0 472 1236
Size: 472 764
MslI CAynn'nnrTG
Cuts at: 0 689 1039 1236
Size: 689 350 197
Fragments arranged by size:
689 350 197
MspA1I CmG'CkG
Cuts at: 0 222 672 1236
Size: 222 450 564
Fragments arranged by size:
564 450 222
NspI r_CATG'y
Cuts at: 0 99 310 446 1236
Size: 99 211 136 790
Fragments arranged by size:
790 211 136 99
PciI A'CATG_T
Cuts at: 0 95 442 1236
Size: 95 347 794
Fragments arranged by size:
794 347 95
PflMI CCAn_nnn'nTGG
Cuts at: 0 531 1236
Size: 531 705
PfoI T'CCnGG_A
Cuts at: 0 562 1236
Size: 562 674
PpuMI rG'GwC_Cy
Cuts at: 0 492 640 1236
Size: 492 148 596
Fragments arranged by size:
596 492 148
PspOMI G'GGCC_C
Cuts at: 0 936 1236
Size: 936 300
PspXI vC'TCGA_Gb
Cuts at: 0 687 1236
Size: 687 549
PstI C_TGCA'G
Cuts at: 0 365 551 1236
Size: 365 186 685
Fragments arranged by size:
685 365 186
PvuII CAG'CTG
Cuts at: 0 222 1236
Size: 222 1014
SalI G'TCGA_C
Cuts at: 0 1171 1236
Size: 1171 65
SfcI C'TryA_G
Cuts at: 0 361 547 1236
Size: 361 186 689
Fragments arranged by size:
689 361 186
SmaI CCC'GGG
Cuts at: 0 451 1236
Size: 451 785
SmlI C'TyrA_G
Cuts at: 0 687 1236
Size: 687 549
StuI AGG'CCT
Cuts at: 0 1061 1236
Size: 1061 175
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 532 959 1236
Size: 532 427 277
Fragments arranged by size:
532 427 277
TatI w'GTAC_w
Cuts at: 0 107 1236
Size: 107 1129
XhoI C'TCGA_G
Cuts at: 0 687 1236
Size: 687 549
XmaI C'CCGG_G
Cuts at: 0 449 1236
Size: 449 787
Enzymes that do cut:
AccI Acc65I AcuI AflIII AhdI AleI AlwNI ApaI
AvaI BamHI BanI BanII BbsI BciVI BclI BglI
BglII Bme1580I BmgBI BpmI Bpu10I BsaI BsaWI BseRI
BseYI BsiHKAI BsmBI Bsp1286I BspHI BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstXI BstYI BtgI BtgZI
BtsI DraI DraIII DrdI EaeI EarI Eco57MI EcoO109I
FalI FspI Hin4I HincII KpnI MscI MslI MspA1I
NspI PciI PflMI PfoI PpuMI PspOMI PspXI PstI
PvuII SalI SfcI SmaI SmlI StuI TaqII TatI
XhoI XmaI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AlfI AloI
ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbeI
BbvCI BcgI BfrBI BlpI BmrI BmtI BplI BpuEI
BsaAI BsaBI BsaHI BsaXI BsgI BsiEI BsiWI BsmI
BspEI BstAPI BstBI BstEII BstZ17I Bsu36I ClaI CspCI
EagI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspAI
HaeII HindIII HpaI KasI MfeI MluI MmeI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PmeI PmlI PpiI PshAI PsiI PsrI
PvuI RsrII SacI SacII SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StyI SwaI TstI Tth111I XbaI XcmI
XmnI ZraI