Restriction map for
pFN21AB8282
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# NO CUT ENZYMES ( 152 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AvaII AviII AvrII BalI
BamHI BbeI BbrPI BbvCI BcgI
BciVI BclI BfaI BfrI BglI
BglII BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsgI
BsiEI BsiWI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrDI BsrFI BsrGI
BsrI Bst1107I BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI HaeII HgaI HindIII
HpaI KasI KpnI KspI MaeI
MamI MflI MluI MluNI MroI
MscI MslI NaeI NarI NcoI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AatII 28 GACGTC GACGT^C
AccII 424 CGCG CG^CG
AcyI 28 GACGTC G[GA]^CG[CT]C
ApaI 829 GGGCCC GGGCC^C
AspHI 526 GTGCTC G[AT]GC[AT]^C
AspI 109 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 418 CCCGAG C^[CT]CG[GA]G
BanI 715 GGCACC G^G[CT][GA]CC
BanII 829 GGGCCC G[GA]GC[CT]^C
BsaHI 28 GACGTC G[GA]^CG[CT]C
BsaI 891 GAGACC GGTCTC(1/5)
BsiHKAI 526 GTGCTC G[AT]GC[AT]^C
BsmBI 745 GAGACG CGTCTC(1/5)
BsmFI 26 GGGAC GGGAC(10/14)
BsmI 619 GAATGC GAATGC(1/-1)
BsoBI 418 CCCGAG C^[CT]CG[GA]G
BspHI 155 TCATGA T^CATGA
BsrBI 787 GAGCGG CCGCTC(-3/-3)
BssHII 423 GCGCGC G^CGCGC
BssSI 888 CACGAG CACGAG(-5/-1)
BstAPI 437 GCAGTATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 424 CGCG CG^CG
DsaI 481 CCGTGG C^C[GA][CT]GG
EaeI 266 TGGCCG [CT]^GGCC[GA]
Hin1I 28 GACGTC G[GA]^CG[CT]C
HincII 241 GTCAAC GT[CT]^[GA]AC
HindII 241 GTCAAC GT[CT]^[GA]AC
MfeI 492 CAATTG C^AATTG
MspA1I 364 CAGCTG C[AC]G^C[GT]G
MunI 492 CAATTG C^AATTG
MvnI 424 CGCG CG^CG
NdeI 732 CATATG CA^TATG
PflFI 109 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 70 GACTC GAGTC(4/5)
PshAI 195 GACTTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 364 CAGCTG CAG^CTG
RcaI 155 TCATGA T^CATGA
SapI 528 GCTCTTC GCTCTTC(1/4)
SfaNI 36 GCATC GCATC(5/9)
SmlI 673 CTTGAG C^T[CT][GA]AG
Tsp509I 493 AATT ^AATT
Tth111I 109 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AfaI 231 GTAC GT^AC
322 GTAC
AlwI 570 GATCC GGATC(4/5)
468 GGATC
BbsI 552 GTCTTC GAAGAC(2/6)
680 GTCTTC
BpuAI 552 GTCTTC GAAGAC(2/6)
680 GTCTTC
BsmAI 745 GAGAC GTCTC(1/5)
891 GAGAC
CfoI 423 GCGC GCG^C
425 GCGC
DdeI 292 CTGAG C^T[ACGT]AG
918 CTCAG
DraII 828 GGGGCCC [GA]G^G[ACGT]CC[CT]
829 GGGCCCC
EarI 72 CTCTTC CTCTTC(1/4)
529 CTCTTC
EcoO109I 828 GGGGCCC [GA]G^G[ACGT]CC[CT]
829 GGGCCCC
HhaI 423 GCGC GCG^C
425 GCGC
HinP1I 423 GCGC G^CGC
425 GCGC
Ksp632I 72 CTCTTC CTCTTC(1/4)
529 CTCTTC
MaeIII 128 GTGAC ^GT[ACGT]AC
193 GTGAC
MseI 257 TTAA T^TAA
413 TTAA
PstI 435 CTGCAG CTGCA^G
701 CTGCAG
RsaI 231 GTAC GT^AC
322 GTAC
SfcI 435 CTGCAG C^T[GA][CT]AG
701 CTGCAG
TaqI 32 TCGA T^CGA
755 TCGA
TfiI 510 GAATC G^A[AT]TC
852 GAATC
Tru9I 257 TTAA T^TAA
413 TTAA
Tsp45I 128 GTGAC ^GT[GC]AC
193 GTGAC
TthHB8I 32 TCGA T^CGA
755 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AluI BbvI BcnI BmyI
BsaJI BseRI BsiYI BslI Bsp1286I
BssKI BstF5I BstNI Cac8I Cfr13I
DpnII EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeII MboI MboII MnlI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI TseI
TspRI