(Circular) (Six-base) MAPSORT of: pFN21AB8282.seq Check: 7291 from: 1 to: 5629 pFN21AB8282 5629 bp With 182 enzymes: SgfI * February 27, 2009 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2037 3439 278 Size: 53 83 186 936 501 1402 2468 Fragments arranged by size: 2468 1402 936 501 186 83 53 AccI GT'mk_AC Cuts at: 1937 2972 1937 Size: 1035 4594 Acc65I G'GTAC_C Cuts at: 2950 2950 Size: 5629 AclI AA'CG_TT Cuts at: 1563 3756 4129 1563 Size: 2193 373 3063 Fragments arranged by size: 3063 2193 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2393 2544 3692 5076 5344 1632 Size: 761 151 1148 1384 268 1917 Fragments arranged by size: 1917 1384 1148 761 268 151 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5432 AflIII A'CryG_T Cuts at: 1203 4528 5505 1203 Size: 3325 977 1327 Fragments arranged by size: 3325 1327 977 AgeI A'CCGG_T Cuts at: 4460 4460 Size: 5629 AhdI GACnn_n'nnGTC Cuts at: 4358 4358 Size: 5629 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5629 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5629 AlwNI CAG_nnn'CTG Cuts at: 3372 4944 3372 Size: 1572 4057 ApaI G_GGCC'C Cuts at: 2838 2838 Size: 5629 ApaLI G'TGCA_C Cuts at: 3686 4842 3686 Size: 1156 4473 ApoI r'AATT_y Cuts at: 1493 2938 3164 3316 5425 1493 Size: 1445 226 152 2109 1697 Fragments arranged by size: 2109 1697 1445 226 152 AseI AT'TA_AT Cuts at: 160 1032 4183 160 Size: 872 3151 1606 Fragments arranged by size: 3151 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5629 AvaI C'yCGr_G Cuts at: 1870 1943 2423 2954 1870 Size: 73 480 531 4545 Fragments arranged by size: 4545 531 480 73 BamHI G'GATC_C Cuts at: 2959 3326 2959 Size: 367 5262 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2720 2950 4405 618 Size: 325 217 627 933 230 1455 1842 Fragments arranged by size: 1842 1455 933 627 325 230 217 BanII G_rGCy'C Cuts at: 1391 2838 2948 1391 Size: 1447 110 4072 Fragments arranged by size: 4072 1447 110 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2550 2678 928 Size: 971 651 128 3879 Fragments arranged by size: 3879 971 651 128 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3820 3854 1903 Size: 34 50 34 1799 34 3678 Fragments arranged by size: 3678 1799 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3521 4731 3521 Size: 1210 4419 BclI T'GATC_A Cuts at: 1548 1548 Size: 5629 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4240 136 Size: 107 122 71 631 605 2568 1525 Fragments arranged by size: 2568 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5624 5624 Size: 5629 BlpI GC'TnA_GC Cuts at: 3046 3046 Size: 5629 Bme1580I G_kGCm'C Cuts at: 1790 2402 2838 3690 4846 1790 Size: 612 436 852 1156 2573 Fragments arranged by size: 2573 1156 852 612 436 BmgBI CAC'GTC Cuts at: 1318 2107 2166 1318 Size: 789 59 4781 Fragments arranged by size: 4781 789 59 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3360 4318 442 Size: 416 53 83 399 24 1943 958 1753 Fragments arranged by size: 1943 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5629 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4289 1239 Size: 3050 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2699 3754 4619 4917 2699 Size: 1055 865 298 3411 Fragments arranged by size: 3411 1055 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2890 4292 882 Size: 617 1391 1402 2219 Fragments arranged by size: 2219 1402 1391 617 BsaAI yAC'GTr Cuts at: 493 1204 3420 493 Size: 711 2216 2702 Fragments arranged by size: 2702 2216 711 BsaBI GATnn'nnATC Cuts at: 5623 5623 Size: 5629 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2034 3436 3818 4453 Size: 53 83 186 936 501 1402 382 635 Cuts at: 4453 275 Size: 1451 Fragments arranged by size: 1451 1402 936 635 501 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4062 4460 4734 4881 5292 4062 Size: 398 274 147 411 4399 Fragments arranged by size: 4399 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2010 2040 1178 Size: 30 165 30 607 30 4767 Fragments arranged by size: 4767 607 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2080 2083 2837 1186 Size: 894 3 754 3978 Fragments arranged by size: 3978 894 754 3 BseYI C'CCAG_C Cuts at: 4832 5328 4832 Size: 496 5133 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5056 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3079 3840 4868 1540 Size: 275 186 1078 761 1028 2301 Fragments arranged by size: 2301 1078 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2535 3690 3775 4846 2535 Size: 1155 85 1071 3318 Fragments arranged by size: 3318 1155 1071 85 BsmI GAATG_Cn' Cuts at: 2630 3140 3233 2630 Size: 510 93 5026 Fragments arranged by size: 5026 510 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2744 2744 Size: 5629 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2402 2535 2838 2948 3690 3775 4846 Size: 399 612 133 303 110 742 85 1071 Cuts at: 4846 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 612 399 303 133 110 85 BspEI T'CCGG_A Cuts at: 5292 5292 Size: 5629 BspHI T'CATG_A Cuts at: 2160 3518 5248 2160 Size: 1358 1730 2541 Fragments arranged by size: 2541 1730 1358 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2984 844 Size: 2140 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2794 3516 1394 Size: 429 971 722 3507 Fragments arranged by size: 3507 971 722 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4124 4298 65 Size: 1183 2876 174 1396 Fragments arranged by size: 2876 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4273 4460 1700 Size: 110 2463 187 2869 Fragments arranged by size: 2869 2463 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5629 BssHII G'CGCG_C Cuts at: 1925 2428 1925 Size: 503 5126 BssSI C'ACGA_G Cuts at: 2893 3689 4701 2893 Size: 796 1012 3821 Fragments arranged by size: 3821 1012 796 BstAPI GCAn_nnn'nTGC Cuts at: 794 2448 794 Size: 1654 3975 BstBI TT'CG_AA Cuts at: 4433 4433 Size: 5629 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5629 BstYI r'GATC_y Cuts at: 1972 2959 3326 3711 3728 5180 5266 5278 5624 Size: 987 367 385 17 1452 86 12 346 Cuts at: 5624 1972 Size: 1977 Fragments arranged by size: 1977 1452 987 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2486 513 Size: 552 1421 3656 Fragments arranged by size: 3656 1421 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2818 487 Size: 683 56 1592 3298 Fragments arranged by size: 3298 1592 683 56 BtsI GCAGTG_nn' Cuts at: 2437 2479 2621 2635 2703 3145 3939 3959 2437 Size: 42 142 14 68 442 794 20 4107 Fragments arranged by size: 4107 794 442 142 68 42 20 14 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5629 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5594 DraI TTT'AAA Cuts at: 2933 2933 Size: 5629 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4636 809 Size: 507 3320 1802 Fragments arranged by size: 3320 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2271 3076 3965 Size: 54 1425 50 275 18 441 805 889 Cuts at: 3965 5594 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 805 441 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3076 1537 Size: 275 1264 4090 Fragments arranged by size: 4090 1264 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2083 2540 3559 1353 Size: 730 457 1019 3423 Fragments arranged by size: 3423 1019 730 457 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4216 4590 4736 387 Size: 1064 138 2627 374 146 1280 Fragments arranged by size: 2627 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2393 2544 3692 4289 5076 5344 1239 Size: 393 761 151 1148 597 787 268 1524 Fragments arranged by size: 1524 1148 787 761 597 393 268 151 EcoO109I rG'GnC_Cy Cuts at: 2834 2835 2834 Size: 1 5628 EcoRI G'AATT_C Cuts at: 2938 3316 2938 Size: 378 5251 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2842 2842 Size: 5629 FspI TGC'GCA Cuts at: 4135 4135 Size: 5629 HaeII r_GCGC'y Cuts at: 1678 4776 1678 Size: 3098 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2010 2042 2066 2098 4270 Size: 32 135 32 438 32 24 32 2172 Cuts at: 4270 4302 4344 4376 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2172 438 135 42 32 32 32 32 32 32 24 HincII GTy'rAC Cuts at: 669 1657 1938 2248 2973 3219 669 Size: 988 281 310 725 246 3079 Fragments arranged by size: 3079 988 725 310 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5629 HpaI GTT'AAC Cuts at: 3219 3219 Size: 5629 KpnI G_GTAC'C Cuts at: 2954 2954 Size: 5629 MfeI C'AATT_G Cuts at: 2497 3228 2497 Size: 731 4898 MluI A'CGCG_T Cuts at: 5505 5505 Size: 5629 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4743 4927 1305 Size: 3438 184 2007 Fragments arranged by size: 3438 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3807 Fragments arranged by size: 3807 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3587 3946 4105 518 Size: 648 2421 359 159 2042 Fragments arranged by size: 2421 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2371 3045 3722 4870 5115 1606 Size: 765 674 677 1148 245 2120 Fragments arranged by size: 2120 1148 765 677 674 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5077 NdeI CA'TA_TG Cuts at: 387 2738 387 Size: 2351 3278 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5629 NotI GC'GGCC_GC Cuts at: 3076 3076 Size: 5629 NspI r_CATG'y Cuts at: 1132 1229 2987 4532 1132 Size: 97 1758 1545 2229 Fragments arranged by size: 2229 1758 1545 97 PasI CC'CwG_GG Cuts at: 1348 2318 1348 Size: 970 4659 PciI A'CATG_T Cuts at: 4528 4528 Size: 5629 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5629 PfoI T'CCnGG_A Cuts at: 2028 2028 Size: 5629 PmeI GTTT'AAAC Cuts at: 2933 2933 Size: 5629 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3698 3730 5211 5243 3698 Size: 32 1481 32 4084 Fragments arranged by size: 4084 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2204 1613 Size: 591 5038 PsiI TTA'TAA Cuts at: 3199 3199 Size: 5629 PspOMI G'GGCC_C Cuts at: 2834 2834 Size: 5629 PstI C_TGCA'G Cuts at: 830 2444 2710 2981 830 Size: 1614 266 271 3478 Fragments arranged by size: 3478 1614 271 266 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5629 PvuII CAG'CTG Cuts at: 2371 2371 Size: 5629 SalI G'TCGA_C Cuts at: 1936 2971 1936 Size: 1035 4594 SapI GCTCTTCn'nnn_ Cuts at: 2540 2540 Size: 5629 SbfI CC_TGCA'GG Cuts at: 2981 2981 Size: 5629 ScaI AGT'ACT Cuts at: 3877 5589 3877 Size: 1712 3917 SfcI C'TryA_G Cuts at: 826 1045 2440 2706 2977 4112 4793 4984 826 Size: 219 1395 266 271 1135 681 191 1471 Fragments arranged by size: 1471 1395 1135 681 271 266 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5629 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5629 SmaI CCC'GGG Cuts at: 1872 2956 1872 Size: 1084 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2678 3733 4634 4896 820 Size: 197 926 735 1055 901 262 1553 Fragments arranged by size: 1553 1055 926 901 735 262 197 SnaBI TAC'GTA Cuts at: 493 3420 493 Size: 2927 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5629 SphI G_CATG'C Cuts at: 1132 2987 1132 Size: 1855 3774 SspI AAT'ATT Cuts at: 5 52 3553 5 Size: 47 3501 2081 Fragments arranged by size: 3501 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5629 StyI C'CwwG_G Cuts at: 513 1065 3068 513 Size: 552 2003 3074 Fragments arranged by size: 3074 2003 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2479 2738 3656 3673 3826 4011 4456 Size: 1255 899 259 918 17 153 185 445 Cuts at: 4456 325 Size: 1498 Fragments arranged by size: 1498 1255 918 899 445 259 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3875 5587 96 Size: 274 80 33 51 1443 1898 1712 138 Fragments arranged by size: 1898 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5589 5621 5589 Size: 32 5597 Tth111I GACn'n_nGTC Cuts at: 1316 2117 1316 Size: 801 4828 XbaI T'CTAG_A Cuts at: 2965 2965 Size: 5629 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5629 XmaI C'CCGG_G Cuts at: 1870 2954 1870 Size: 1084 4545 XmnI GAAnn'nnTTC Cuts at: 3758 3758 Size: 5629 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2035 3437 276 Size: 53 83 186 936 501 1402 2468 Fragments arranged by size: 2468 1402 936 501 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI