Restriction map for
pFN21AB8282
(Linear) (Six-base) MAPSORT of: KIBB8282.seq Check: 5082 from: 1 to: 924
KIBB8282.orf
With 182 enzymes: SgfI *
March 3, 2008 11:04 ..
AatII G_ACGT'C
Cuts at: 0 32 924
Size: 32 892
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 388 539 924
Size: 388 151 385
Fragments arranged by size:
388 385 151
ApaI G_GGCC'C
Cuts at: 0 833 924
Size: 833 91
AvaI C'yCGr_G
Cuts at: 0 418 924
Size: 418 506
BanI G'GyrC_C
Cuts at: 0 715 924
Size: 715 209
BanII G_rGCy'C
Cuts at: 0 833 924
Size: 833 91
BbsI GAAGACnn'nnnn_
Cuts at: 0 545 673 924
Size: 545 128 251
Fragments arranged by size:
545 251 128
Bme1580I G_kGCm'C
Cuts at: 0 397 833 924
Size: 397 436 91
Fragments arranged by size:
436 397 91
BmgBI CAC'GTC
Cuts at: 0 102 161 924
Size: 102 59 763
Fragments arranged by size:
763 102 59
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 694 924
Size: 694 230
BsaI GGTCTCn'nnnn_
Cuts at: 0 885 924
Size: 885 39
BsaHI Gr'CG_yC
Cuts at: 0 29 924
Size: 29 895
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 5 35 924
Size: 5 30 889
Fragments arranged by size:
889 30 5
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 75 78 832 924
Size: 75 3 754 92
Fragments arranged by size:
754 92 75 3
BsiHKAI G_wGCw'C
Cuts at: 0 530 924
Size: 530 394
BsmI GAATG_Cn'
Cuts at: 0 625 924
Size: 625 299
BsmBI CGTCTCn'nnnn_
Cuts at: 0 739 924
Size: 739 185
Bsp1286I G_dGCh'C
Cuts at: 0 397 530 833 924
Size: 397 133 303 91
Fragments arranged by size:
397 303 133 91
BspHI T'CATG_A
Cuts at: 0 155 924
Size: 155 769
BsrBI CCG'CTC
Cuts at: 0 789 924
Size: 789 135
BssHII G'CGCG_C
Cuts at: 0 423 924
Size: 423 501
BssSI C'ACGA_G
Cuts at: 0 888 924
Size: 888 36
BstAPI GCAn_nnn'nTGC
Cuts at: 0 443 924
Size: 443 481
BtgI C'CryG_G
Cuts at: 0 481 924
Size: 481 443
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 813 924
Size: 813 111
BtsI GCAGTG_nn'
Cuts at: 0 432 474 616 630 698 924
Size: 432 42 142 14 68 226
Fragments arranged by size:
432 226 142 68 42 14
EaeI y'GGCC_r
Cuts at: 0 266 924
Size: 266 658
EarI CTCTTCn'nnn_
Cuts at: 0 78 535 924
Size: 78 457 389
Fragments arranged by size:
457 389 78
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 388 539 924
Size: 388 151 385
Fragments arranged by size:
388 385 151
EcoO109I rG'GnC_Cy
Cuts at: 0 829 830 924
Size: 829 1 94
Fragments arranged by size:
829 94 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 837 924
Size: 837 87
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 5 37 61 93 924
Size: 5 32 24 32 831
Fragments arranged by size:
831 32 32 24 5
HincII GTy'rAC
Cuts at: 0 243 924
Size: 243 681
MfeI C'AATT_G
Cuts at: 0 492 924
Size: 492 432
MspA1I CmG'CkG
Cuts at: 0 366 924
Size: 366 558
NdeI CA'TA_TG
Cuts at: 0 733 924
Size: 733 191
PasI CC'CwG_GG
Cuts at: 0 313 924
Size: 313 611
PfoI T'CCnGG_A
Cuts at: 0 23 924
Size: 23 901
PshAI GACnn'nnGTC
Cuts at: 0 199 924
Size: 199 725
PspOMI G'GGCC_C
Cuts at: 0 829 924
Size: 829 95
PstI C_TGCA'G
Cuts at: 0 439 705 924
Size: 439 266 219
Fragments arranged by size:
439 266 219
PvuII CAG'CTG
Cuts at: 0 366 924
Size: 366 558
SapI GCTCTTCn'nnn_
Cuts at: 0 535 924
Size: 535 389
SfcI C'TryA_G
Cuts at: 0 435 701 924
Size: 435 266 223
Fragments arranged by size:
435 266 223
SmlI C'TyrA_G
Cuts at: 0 673 924
Size: 673 251
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 474 733 924
Size: 474 259 191
Fragments arranged by size:
474 259 191
Tth111I GACn'n_nGTC
Cuts at: 0 112 924
Size: 112 812
ZraI GAC'GTC
Cuts at: 0 30 924
Size: 30 894
Enzymes that do cut:
AatII AcuI ApaI AvaI BanI BanII BbsI Bme1580I
BmgBI BpuEI BsaI BsaHI BsaXI BseRI BsiHKAI BsmI
BsmBI Bsp1286I BspHI BsrBI BssHII BssSI BstAPI BtgI
BtgZI BtsI EaeI EarI Eco57MI EcoO109I FalI Hin4I
HincII MfeI MspA1I NdeI PasI PfoI PshAI PspOMI
PstI PvuII SapI SfcI SmlI TaqII Tth111I ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI BmrI BmtI
BplI BpmI Bpu10I BsaAI BsaBI BsaWI BseYI BsgI
BsiEI BsiWI BspEI BspMI BsrDI BsrFI BsrGI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I ClaI CspCI DraI
DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV
FseI FspI FspAI HaeII HindIII HpaI KasI KpnI
MluI MmeI MscI MslI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI NspI PacI PciI PflMI
PmeI PmlI PpiI PpuMI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI StyI SwaI TatI TstI XbaI
XcmI XhoI XmaI XmnI