(Circular) (Six-base) MAPSORT of: pFN21AB8281.seq Check: 661 from: 1 to: 5620 pFN21AB8281 5620 bp With 182 enzymes: SgfI * February 27, 2009 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3430 278 Size: 53 83 186 936 1894 2468 Fragments arranged by size: 2468 1894 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2963 1937 Size: 1026 4594 Acc65I G'GTAC_C Cuts at: 2941 2941 Size: 5620 AclI AA'CG_TT Cuts at: 1563 3747 4120 1563 Size: 2184 373 3063 Fragments arranged by size: 3063 2184 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2684 3683 5067 5335 1632 Size: 1052 999 1384 268 1917 Fragments arranged by size: 1917 1384 1052 999 268 AfeI AGC'GCT Cuts at: 2206 2206 Size: 5620 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5423 AflIII A'CryG_T Cuts at: 1203 4519 5496 1203 Size: 3316 977 1327 Fragments arranged by size: 3316 1327 977 AgeI A'CCGG_T Cuts at: 4451 4451 Size: 5620 AhdI GACnn_n'nnGTC Cuts at: 4349 4349 Size: 5620 AleI CACnn'nnGTG Cuts at: 1166 2489 2714 1166 Size: 1323 225 4072 Fragments arranged by size: 4072 1323 225 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5620 AlwNI CAG_nnn'CTG Cuts at: 3363 4935 3363 Size: 1572 4048 ApaLI G'TGCA_C Cuts at: 2492 3677 4833 2492 Size: 1185 1156 3279 Fragments arranged by size: 3279 1185 1156 ApoI r'AATT_y Cuts at: 1493 2929 3155 3307 5416 1493 Size: 1436 226 152 2109 1697 Fragments arranged by size: 2109 1697 1436 226 152 AseI AT'TA_AT Cuts at: 160 1032 4174 160 Size: 872 3142 1606 Fragments arranged by size: 3142 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5620 AvaI C'yCGr_G Cuts at: 1870 1943 2833 2872 2916 2945 1870 Size: 73 890 39 44 29 4545 Fragments arranged by size: 4545 890 73 44 39 29 AvrII C'CTAG_G Cuts at: 2732 2732 Size: 5620 BamHI G'GATC_C Cuts at: 2950 3317 2950 Size: 367 5253 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2941 4396 618 Size: 325 217 627 1154 1455 1842 Fragments arranged by size: 1842 1455 1154 627 325 217 BanII G_rGCy'C Cuts at: 1391 2080 2471 2939 1391 Size: 689 391 468 4072 Fragments arranged by size: 4072 689 468 391 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4649 BbvCI CC'TCA_GC Cuts at: 2305 2305 Size: 5620 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3811 3845 1903 Size: 34 1874 34 3678 Fragments arranged by size: 3678 1874 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3512 4722 3512 Size: 1210 4410 BclI T'GATC_A Cuts at: 1548 1548 Size: 5620 BfrBI ATG'CAT Cuts at: 2596 2596 Size: 5620 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2657 2720 2791 Size: 107 122 71 631 605 985 63 71 Cuts at: 2791 4231 136 Size: 1440 1525 Fragments arranged by size: 1525 1440 985 631 605 122 107 71 71 63 BglII A'GATC_T Cuts at: 5615 5615 Size: 5620 BlpI GC'TnA_GC Cuts at: 3037 3037 Size: 5620 Bme1580I G_kGCm'C Cuts at: 1790 2496 3681 4837 1790 Size: 706 1185 1156 2573 Fragments arranged by size: 2573 1185 1156 706 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5620 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3351 4309 442 Size: 416 53 83 399 24 1934 958 1753 Fragments arranged by size: 1934 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5620 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2490 2490 Size: 5620 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2248 2278 2314 2332 2884 4280 1239 Size: 1009 30 36 18 552 1396 2579 Fragments arranged by size: 2579 1396 1009 552 36 30 18 Bpu10I CC'TnA_GC Cuts at: 2257 2305 2320 2478 2257 Size: 48 15 158 5399 Fragments arranged by size: 5399 158 48 15 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2266 3745 4610 4908 2266 Size: 1479 865 298 2978 Fragments arranged by size: 2978 1479 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2238 2805 4283 882 Size: 617 739 567 1478 2219 Fragments arranged by size: 2219 1478 739 617 567 BsaAI yAC'GTr Cuts at: 493 1204 2626 3411 493 Size: 711 1422 785 2702 Fragments arranged by size: 2702 1422 785 711 BsaBI GATnn'nnATC Cuts at: 5614 5614 Size: 5620 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3427 3809 4444 275 Size: 53 83 186 936 1894 382 635 1451 Fragments arranged by size: 1894 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4053 4451 4725 4872 5283 4053 Size: 398 274 147 411 4390 Fragments arranged by size: 4390 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2062 2092 1178 Size: 30 165 30 659 30 4706 Fragments arranged by size: 4706 659 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2830 2902 1186 Size: 1644 72 3904 Fragments arranged by size: 3904 1644 72 BseYI C'CCAG_C Cuts at: 4823 5319 4823 Size: 496 5124 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5047 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2179 3070 3831 4859 1540 Size: 275 186 178 891 761 1028 2301 Fragments arranged by size: 2301 1028 891 761 275 186 178 BsiHKAI G_wGCw'C Cuts at: 2496 3681 3766 4837 2496 Size: 1185 85 1071 3279 Fragments arranged by size: 3279 1185 1071 85 BsiWI C'GTAC_G Cuts at: 2622 2622 Size: 5620 BsmI GAATG_Cn' Cuts at: 3131 3224 3131 Size: 93 5527 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2080 2471 2496 2939 3681 3766 4837 Size: 399 290 391 25 443 742 85 1071 Cuts at: 4837 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 443 399 391 290 85 25 BspEI T'CCGG_A Cuts at: 5283 5283 Size: 5620 BspHI T'CATG_A Cuts at: 3509 5239 3509 Size: 1730 3890 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2787 2832 2975 844 Size: 1943 45 143 3489 Fragments arranged by size: 3489 1943 143 45 BsrBI CCG'CTC Cuts at: 1394 1823 2106 2319 2418 2829 2898 3507 1394 Size: 429 283 213 99 411 69 609 3507 Fragments arranged by size: 3507 609 429 411 283 213 99 69 BsrDI GCAATG_nn' Cuts at: 65 1248 4115 4289 65 Size: 1183 2867 174 1396 Fragments arranged by size: 2867 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4264 4451 1700 Size: 110 2454 187 2869 Fragments arranged by size: 2869 2454 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5620 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5620 BssSI C'ACGA_G Cuts at: 3680 4692 3680 Size: 1012 4608 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5620 BstBI TT'CG_AA Cuts at: 4424 4424 Size: 5620 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5620 BstYI r'GATC_y Cuts at: 1972 2950 3317 3702 3719 5171 5257 5269 5615 Size: 978 367 385 17 1452 86 12 346 Cuts at: 5615 1972 Size: 1977 Fragments arranged by size: 1977 1452 978 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2583 2807 513 Size: 552 939 579 224 3326 Fragments arranged by size: 3326 939 579 552 224 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4881 Fragments arranged by size: 4881 683 56 BtsI GCAGTG_nn' Cuts at: 2293 2703 3136 3930 3950 2293 Size: 410 433 794 20 3963 Fragments arranged by size: 3963 794 433 410 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5620 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5585 DraI TTT'AAA Cuts at: 2924 2924 Size: 5620 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4627 809 Size: 507 3311 1802 Fragments arranged by size: 3311 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2176 2692 3067 Size: 54 1425 50 275 18 346 516 375 Cuts at: 3067 3956 5585 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 516 375 346 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2176 3067 1537 Size: 275 364 891 4090 Fragments arranged by size: 4090 891 364 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2024 2305 2368 3550 1353 Size: 671 281 63 1182 3423 Fragments arranged by size: 3423 1182 671 281 63 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2479 4207 4581 4727 387 Size: 1064 138 890 1728 374 146 1280 Fragments arranged by size: 1728 1280 1064 890 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2248 2278 2314 2332 2684 2884 3683 Size: 393 616 30 36 18 352 200 799 Cuts at: 3683 4280 5067 5335 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 799 787 616 597 393 352 268 200 36 30 18 EcoNI CCTnn'n_nnAGG Cuts at: 2731 2731 Size: 5620 EcoO109I rG'GnC_Cy Cuts at: 2823 2844 2823 Size: 21 5599 EcoRI G'AATT_C Cuts at: 2929 3307 2929 Size: 378 5242 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2050 2050 Size: 5620 FspI TGC'GCA Cuts at: 4126 4126 Size: 5620 HaeII r_GCGC'y Cuts at: 1678 2208 2413 2663 4767 1678 Size: 530 205 250 2104 2531 Fragments arranged by size: 2531 2104 530 250 205 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2102 2134 2512 2544 4261 Size: 32 135 32 530 32 378 32 1717 Cuts at: 4261 4293 4335 4367 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1717 530 378 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2964 3210 669 Size: 988 281 1026 246 3079 Fragments arranged by size: 3079 1026 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5620 HpaI GTT'AAC Cuts at: 3210 3210 Size: 5620 KpnI G_GTAC'C Cuts at: 2945 2945 Size: 5620 MfeI C'AATT_G Cuts at: 3219 3219 Size: 5620 MluI A'CGCG_T Cuts at: 5496 5496 Size: 5620 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2931 4734 4918 1305 Size: 1626 1803 184 2007 Fragments arranged by size: 2007 1803 1626 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2694 10 Size: 54 1425 343 862 2936 Fragments arranged by size: 2936 1425 862 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2489 2582 2673 2714 3578 3937 4096 Size: 648 1323 93 91 41 864 359 159 Cuts at: 4096 518 Size: 2042 Fragments arranged by size: 2042 1323 864 648 359 159 93 91 41 MspA1I CmG'CkG Cuts at: 1606 3036 3713 4861 5106 1606 Size: 1430 677 1148 245 2120 Fragments arranged by size: 2120 1430 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4129 Fragments arranged by size: 4129 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5620 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5620 NotI GC'GGCC_GC Cuts at: 3067 3067 Size: 5620 NsiI A_TGCA'T Cuts at: 2598 2598 Size: 5620 NspI r_CATG'y Cuts at: 1132 1229 2581 2978 4523 1132 Size: 97 1352 397 1545 2229 Fragments arranged by size: 2229 1545 1352 397 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5620 PciI A'CATG_T Cuts at: 4519 4519 Size: 5620 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5620 PmeI GTTT'AAAC Cuts at: 2924 2924 Size: 5620 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3689 3721 5202 5234 3689 Size: 32 1481 32 4075 Fragments arranged by size: 4075 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2823 2844 2823 Size: 21 5599 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5620 PsiI TTA'TAA Cuts at: 3190 3190 Size: 5620 PstI C_TGCA'G Cuts at: 830 2972 830 Size: 2142 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5620 SalI G'TCGA_C Cuts at: 1936 2962 1936 Size: 1026 4594 SapI GCTCTTCn'nnn_ Cuts at: 2024 2305 2368 2024 Size: 281 63 5276 Fragments arranged by size: 5276 281 63 SbfI CC_TGCA'GG Cuts at: 2972 2972 Size: 5620 ScaI AGT'ACT Cuts at: 3868 5580 3868 Size: 1712 3908 SexAI A'CCwGG_T Cuts at: 2228 2228 Size: 5620 SfcI C'TryA_G Cuts at: 826 1045 2968 4103 4784 4975 826 Size: 219 1923 1135 681 191 1471 Fragments arranged by size: 1923 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5620 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5620 SmaI CCC'GGG Cuts at: 1872 2874 2947 1872 Size: 1002 73 4545 Fragments arranged by size: 4545 1002 73 SmlI C'TyrA_G Cuts at: 820 1017 1943 2281 3724 4625 4887 820 Size: 197 926 338 1443 901 262 1553 Fragments arranged by size: 1553 1443 926 901 338 262 197 SnaBI TAC'GTA Cuts at: 493 2626 3411 493 Size: 2133 785 2702 Fragments arranged by size: 2702 2133 785 SpeI A'CTAG_T Cuts at: 152 152 Size: 5620 SphI G_CATG'C Cuts at: 1132 2978 1132 Size: 1846 3774 SspI AAT'ATT Cuts at: 5 52 3544 5 Size: 47 3492 2081 Fragments arranged by size: 3492 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5620 StyI C'CwwG_G Cuts at: 513 1065 2004 2121 2151 2732 3059 513 Size: 552 939 117 30 581 327 3074 Fragments arranged by size: 3074 939 581 552 327 117 30 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2226 3647 3664 3817 4002 4447 325 Size: 1255 646 1421 17 153 185 445 1498 Fragments arranged by size: 1498 1421 1255 646 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3866 5578 96 Size: 274 80 33 51 1443 1889 1712 138 Fragments arranged by size: 1889 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2062 2094 5580 5612 2062 Size: 32 3486 32 2070 Fragments arranged by size: 3486 2070 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5620 XbaI T'CTAG_A Cuts at: 2956 2956 Size: 5620 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2716 2787 2716 Size: 71 5549 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5620 XmaI C'CCGG_G Cuts at: 1870 2872 2945 1870 Size: 1002 73 4545 Fragments arranged by size: 4545 1002 73 XmnI GAAnn'nnTTC Cuts at: 3749 3749 Size: 5620 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3428 276 Size: 53 83 186 936 1894 2468 Fragments arranged by size: 2468 1894 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI BaeI BbeI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SfiI SfoI SrfI SwaI