(Circular) (Six-base) MAPSORT of: pFN21AB8270.seq Check: 967 from: 1 to: 5512 pFN21AB8270 5512 bp With 182 enzymes: SgfI * February 27, 2009 14:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2132 2132 Size: 5512 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2718 3322 278 Size: 53 83 186 936 1182 604 2468 Fragments arranged by size: 2468 1182 936 604 186 83 53 AccI GT'mk_AC Cuts at: 1937 2855 1937 Size: 918 4594 Acc65I G'GTAC_C Cuts at: 2833 2833 Size: 5512 AclI AA'CG_TT Cuts at: 1563 3639 4012 1563 Size: 2076 373 3063 Fragments arranged by size: 3063 2076 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2192 2210 2771 3575 4959 5227 1632 Size: 560 18 561 804 1384 268 1917 Fragments arranged by size: 1917 1384 804 561 560 268 18 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5315 AflIII A'CryG_T Cuts at: 1203 4411 5388 1203 Size: 3208 977 1327 Fragments arranged by size: 3208 1327 977 AgeI A'CCGG_T Cuts at: 2553 4343 2553 Size: 1790 3722 AhdI GACnn_n'nnGTC Cuts at: 4241 4241 Size: 5512 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5512 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5512 AlwNI CAG_nnn'CTG Cuts at: 3255 4827 3255 Size: 1572 3940 ApaLI G'TGCA_C Cuts at: 3569 4725 3569 Size: 1156 4356 ApoI r'AATT_y Cuts at: 1493 2821 3047 3199 5308 1493 Size: 1328 226 152 2109 1697 Fragments arranged by size: 2109 1697 1328 226 152 AseI AT'TA_AT Cuts at: 160 1032 4066 160 Size: 872 3034 1606 Fragments arranged by size: 3034 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5512 AvaI C'yCGr_G Cuts at: 1870 1943 2276 2708 2837 1870 Size: 73 333 432 129 4545 Fragments arranged by size: 4545 432 333 129 73 BamHI G'GATC_C Cuts at: 2842 3209 2842 Size: 367 5145 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2048 2160 2564 2833 4288 Size: 325 217 627 261 112 404 269 1455 Cuts at: 4288 618 Size: 1842 Fragments arranged by size: 1842 1455 627 404 325 269 261 217 112 BanII G_rGCy'C Cuts at: 1391 2283 2582 2831 1391 Size: 892 299 249 4072 Fragments arranged by size: 4072 892 299 249 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2074 2103 2260 928 Size: 971 175 29 157 4180 Fragments arranged by size: 4180 971 175 157 29 BbvCI CC'TCA_GC Cuts at: 2655 2655 Size: 5512 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2461 2495 3703 3737 1903 Size: 34 524 34 1208 34 3678 Fragments arranged by size: 3678 1208 524 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3404 4614 3404 Size: 1210 4302 BclI T'GATC_A Cuts at: 1548 1548 Size: 5512 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2066 4123 Size: 107 122 71 631 605 337 57 2057 Cuts at: 4123 136 Size: 1525 Fragments arranged by size: 2057 1525 631 605 337 122 107 71 57 BglII A'GATC_T Cuts at: 5507 5507 Size: 5512 BlpI GC'TnA_GC Cuts at: 2929 2929 Size: 5512 Bme1580I G_kGCm'C Cuts at: 1790 2051 2163 2291 3573 4729 1790 Size: 261 112 128 1282 1156 2573 Fragments arranged by size: 2573 1282 1156 261 128 112 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5512 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2422 3243 4201 Size: 416 53 83 399 24 1005 821 958 Cuts at: 4201 442 Size: 1753 Fragments arranged by size: 1753 1005 958 821 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5512 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4172 1239 Size: 2933 2579 Bpu10I CC'TnA_GC Cuts at: 2655 2655 Size: 5512 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3637 4502 4800 3637 Size: 865 298 4349 Fragments arranged by size: 4349 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4175 882 Size: 617 2676 2219 Fragments arranged by size: 2676 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3303 493 Size: 711 2099 2702 Fragments arranged by size: 2702 2099 711 BsaBI GATnn'nnATC Cuts at: 5506 5506 Size: 5512 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2715 2733 3319 3701 Size: 53 83 186 936 1182 18 586 382 Cuts at: 3701 4336 275 Size: 635 1451 Fragments arranged by size: 1451 1182 936 635 586 382 186 83 53 18 BsaWI w'CCGG_w Cuts at: 2553 3945 4343 4617 4764 5175 2553 Size: 1392 398 274 147 411 2890 Fragments arranged by size: 2890 1392 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2045 2075 2090 2120 1178 Size: 30 165 30 642 30 15 30 4570 Fragments arranged by size: 4570 642 165 30 30 30 30 15 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2108 1186 Size: 922 4590 BseYI C'CCAG_C Cuts at: 2029 2044 4715 5211 2029 Size: 15 2671 496 2330 Fragments arranged by size: 2671 2330 496 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2727 1163 Size: 573 991 3948 Fragments arranged by size: 3948 991 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2962 3723 4751 1540 Size: 275 186 961 761 1028 2301 Fragments arranged by size: 2301 1028 961 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3573 3658 4729 3573 Size: 85 1071 4356 Fragments arranged by size: 4356 1071 85 BsmI GAATG_Cn' Cuts at: 3023 3116 3023 Size: 93 5419 BsmBI CGTCTCn'nnnn_ Cuts at: 2357 2357 Size: 5512 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2051 2163 2283 2291 2582 2831 3573 Size: 399 261 112 120 8 291 249 742 Cuts at: 3573 3658 4729 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 291 261 249 120 112 85 8 BspEI T'CCGG_A Cuts at: 5175 5175 Size: 5512 BspHI T'CATG_A Cuts at: 2454 3401 5131 2454 Size: 947 1730 2835 Fragments arranged by size: 2835 1730 947 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2132 2463 2867 844 Size: 1288 331 404 3489 Fragments arranged by size: 3489 1288 404 331 BsrBI CCG'CTC Cuts at: 1394 1823 3399 1394 Size: 429 1576 3507 Fragments arranged by size: 3507 1576 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4007 4181 65 Size: 1183 2759 174 1396 Fragments arranged by size: 2759 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2072 2499 2553 2735 4156 4343 1700 Size: 110 262 427 54 182 1421 187 2869 Fragments arranged by size: 2869 1421 427 262 187 182 110 54 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5512 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5512 BssSI C'ACGA_G Cuts at: 3572 4584 3572 Size: 1012 4500 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5512 BstBI TT'CG_AA Cuts at: 4316 4316 Size: 5512 BstEII G'GTnAC_C Cuts at: 1184 2671 1184 Size: 1487 4025 BstYI r'GATC_y Cuts at: 1972 2842 3209 3594 3611 5063 5149 5161 5507 Size: 870 367 385 17 1452 86 12 346 Cuts at: 5507 1972 Size: 1977 Fragments arranged by size: 1977 1452 870 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2053 2283 2308 513 Size: 552 939 49 230 25 3717 Fragments arranged by size: 3717 939 552 230 49 25 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4773 Fragments arranged by size: 4773 683 56 BtsI GCAGTG_nn' Cuts at: 3028 3822 3842 3028 Size: 794 20 4698 Fragments arranged by size: 4698 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5512 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5477 DraI TTT'AAA Cuts at: 2816 2816 Size: 5512 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4519 809 Size: 507 3203 1802 Fragments arranged by size: 3203 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2209 2292 2367 Size: 54 1425 50 275 18 379 83 75 Cuts at: 2367 2545 2622 2959 3848 5477 8 Size: 178 77 337 889 1629 43 Fragments arranged by size: 1629 1425 889 379 337 275 178 83 77 75 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2959 1537 Size: 275 1147 4090 Fragments arranged by size: 4090 1147 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2747 2812 3442 1353 Size: 1394 65 630 3423 Fragments arranged by size: 3423 1394 630 65 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2783 4099 4473 4619 387 Size: 1064 138 1194 1316 374 146 1280 Fragments arranged by size: 1316 1280 1194 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2192 2210 2771 3575 4172 4959 5227 Size: 393 560 18 561 804 597 787 268 Cuts at: 5227 1239 Size: 1524 Fragments arranged by size: 1524 804 787 597 561 560 393 268 18 EcoO109I rG'GnC_Cy Cuts at: 2449 2449 Size: 5512 EcoRI G'AATT_C Cuts at: 2821 3199 2821 Size: 378 5134 FspI TGC'GCA Cuts at: 2612 4018 2612 Size: 1406 4106 HaeII r_GCGC'y Cuts at: 1678 2145 2701 4659 1678 Size: 467 556 1958 2531 Fragments arranged by size: 2531 1958 556 467 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2090 2122 2723 2755 4153 Size: 32 135 32 518 32 601 32 1398 Cuts at: 4153 4185 4227 4259 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1398 601 518 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2856 3102 669 Size: 988 281 918 246 3079 Fragments arranged by size: 3079 988 918 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5512 HpaI GTT'AAC Cuts at: 3102 3102 Size: 5512 KpnI G_GTAC'C Cuts at: 2837 2837 Size: 5512 MfeI C'AATT_G Cuts at: 3111 3111 Size: 5512 MluI A'CGCG_T Cuts at: 5388 5388 Size: 5512 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2607 4626 4810 1305 Size: 1302 2019 184 2007 Fragments arranged by size: 2019 2007 1302 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3690 Fragments arranged by size: 3690 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2772 3470 3829 3988 518 Size: 648 1606 698 359 159 2042 Fragments arranged by size: 2042 1606 698 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2166 2760 2928 3605 4753 4998 1606 Size: 560 594 168 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 594 560 245 168 NaeI GCC'GGC Cuts at: 2074 2737 2074 Size: 663 4849 NcoI C'CATG_G Cuts at: 513 1065 2004 2283 513 Size: 552 939 279 3742 Fragments arranged by size: 3742 939 552 279 NdeI CA'TA_TG Cuts at: 387 387 Size: 5512 NgoMIV G'CCGG_C Cuts at: 2072 2735 2072 Size: 663 4849 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5512 NotI GC'GGCC_GC Cuts at: 2959 2959 Size: 5512 NspI r_CATG'y Cuts at: 1132 1229 2870 4415 1132 Size: 97 1641 1545 2229 Fragments arranged by size: 2229 1641 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5512 PciI A'CATG_T Cuts at: 4411 4411 Size: 5512 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5512 PfoI T'CCnGG_A Cuts at: 2718 2718 Size: 5512 PmeI GTTT'AAAC Cuts at: 2816 2816 Size: 5512 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2459 2491 3581 3613 5094 5126 2459 Size: 32 1090 32 1481 32 2845 Fragments arranged by size: 2845 1481 1090 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5512 PsiI TTA'TAA Cuts at: 3082 3082 Size: 5512 PstI C_TGCA'G Cuts at: 830 2864 830 Size: 2034 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5512 SalI G'TCGA_C Cuts at: 1936 2854 1936 Size: 918 4594 SapI GCTCTTCn'nnn_ Cuts at: 2747 2747 Size: 5512 SbfI CC_TGCA'GG Cuts at: 2864 2864 Size: 5512 ScaI AGT'ACT Cuts at: 2258 3760 5472 2258 Size: 1502 1712 2298 Fragments arranged by size: 2298 1712 1502 SfcI C'TryA_G Cuts at: 826 1045 2860 3995 4676 4867 826 Size: 219 1815 1135 681 191 1471 Fragments arranged by size: 1815 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5512 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5512 SmaI CCC'GGG Cuts at: 1872 2839 1872 Size: 967 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3616 4517 4779 820 Size: 197 926 1673 901 262 1553 Fragments arranged by size: 1673 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3303 493 Size: 2810 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5512 SphI G_CATG'C Cuts at: 1132 2870 1132 Size: 1738 3774 SspI AAT'ATT Cuts at: 5 52 3436 5 Size: 47 3384 2081 Fragments arranged by size: 3384 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5512 StyI C'CwwG_G Cuts at: 513 1065 2004 2101 2283 2574 2951 513 Size: 552 939 97 182 291 377 3074 Fragments arranged by size: 3074 939 552 377 291 182 97 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2066 3539 3556 3709 3894 4339 325 Size: 1255 486 1473 17 153 185 445 1498 Fragments arranged by size: 1498 1473 1255 486 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2256 3758 5470 Size: 274 80 33 51 1443 279 1502 1712 Cuts at: 5470 96 Size: 138 Fragments arranged by size: 1712 1502 1443 279 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2045 2077 2231 2263 5472 5504 2045 Size: 32 154 32 3209 32 2053 Fragments arranged by size: 3209 2053 154 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5512 XbaI T'CTAG_A Cuts at: 2848 2848 Size: 5512 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5512 XmaI C'CCGG_G Cuts at: 1870 2837 1870 Size: 967 4545 XmnI GAAnn'nnTTC Cuts at: 3641 3641 Size: 5512 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2716 3320 276 Size: 53 83 186 936 1182 604 2468 Fragments arranged by size: 2468 1182 936 604 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI