Restriction map for
pFN21AB8266
(Circular) (Six-base) MAPSORT of: pFN21AB8266.seq Check: 5424 from: 1 to: 5275
pFN21AB8266 5275 bp
With 182 enzymes: SgfI *
February 27, 2009 14:41 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3085 278
Size: 53 83 186 936 1549 2468
Fragments arranged by size:
2468 1549 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2618 1937
Size: 681 4594
Acc65I G'GTAC_C
Cuts at: 2596 2596
Size: 5275
AclI AA'CG_TT
Cuts at: 1563 3402 3775 1563
Size: 1839 373 3063
Fragments arranged by size:
3063 1839 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3338 4722 4990 1632
Size: 1706 1384 268 1917
Fragments arranged by size:
1917 1706 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5078
AflIII A'CryG_T
Cuts at: 1203 4174 5151 1203
Size: 2971 977 1327
Fragments arranged by size:
2971 1327 977
AgeI A'CCGG_T
Cuts at: 4106 4106
Size: 5275
AhdI GACnn_n'nnGTC
Cuts at: 4004 4004
Size: 5275
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5275
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5275
AlwNI CAG_nnn'CTG
Cuts at: 3018 4590 3018
Size: 1572 3703
ApaI G_GGCC'C
Cuts at: 2420 2420
Size: 5275
ApaLI G'TGCA_C
Cuts at: 3332 4488 3332
Size: 1156 4119
ApoI r'AATT_y
Cuts at: 1493 2489 2584 2810 2962 5071 1493
Size: 996 95 226 152 2109 1697
Fragments arranged by size:
2109 1697 996 226 152 95
AseI AT'TA_AT
Cuts at: 160 1032 3829 160
Size: 872 2797 1606
Fragments arranged by size:
2797 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5275
AvaI C'yCGr_G
Cuts at: 1870 1943 2158 2600 1870
Size: 73 215 442 4545
Fragments arranged by size:
4545 442 215 73
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 2520 2553 2520
Size: 33 5242
BamHI G'GATC_C
Cuts at: 2605 2972 2605
Size: 367 4908
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2596 4051 618
Size: 325 217 627 809 1455 1842
Fragments arranged by size:
1842 1455 809 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2420 2594 1391
Size: 1029 174 4072
Fragments arranged by size:
4072 1029 174
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2530 928
Size: 971 631 3673
Fragments arranged by size:
3673 971 631
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3466 3500 1903
Size: 34 1529 34 3678
Fragments arranged by size:
3678 1529 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2521 3167 4377 2521
Size: 646 1210 3419
Fragments arranged by size:
3419 1210 646
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5275
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3886 136
Size: 107 122 71 631 605 2214 1525
Fragments arranged by size:
2214 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5270 5270
Size: 5275
BlpI GC'TnA_GC
Cuts at: 2692 2692
Size: 5275
Bme1580I G_kGCm'C
Cuts at: 1790 2420 3336 4492 1790
Size: 630 916 1156 2573
Fragments arranged by size:
2573 1156 916 630
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5275
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2347 3006 3964
Size: 416 53 83 399 24 930 659 958
Cuts at: 3964 442
Size: 1753
Fragments arranged by size:
1753 958 930 659 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5275
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3935 1239
Size: 2696 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3400 4265 4563 3400
Size: 865 298 4112
Fragments arranged by size:
4112 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2092 2267 2363 3938 882
Size: 617 593 175 96 1575 2219
Fragments arranged by size:
2219 1575 617 593 175 96
BsaAI yAC'GTr
Cuts at: 493 1204 3066 493
Size: 711 1862 2702
Fragments arranged by size:
2702 1862 711
BsaBI GATnn'nnATC
Cuts at: 5269 5269
Size: 5275
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3082 3464 4099 275
Size: 53 83 186 936 1549 382 635 1451
Fragments arranged by size:
1549 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2074 2325 3708 4106 4380 4527 4938 2074
Size: 251 1383 398 274 147 411 2411
Fragments arranged by size:
2411 1383 411 398 274 251 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2228 2258 1178
Size: 30 165 30 825 30 4195
Fragments arranged by size:
4195 825 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5275
BseYI C'CCAG_C
Cuts at: 2044 2053 4478 4974 2044
Size: 9 2425 496 2345
Fragments arranged by size:
2425 2345 496 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2114 1163
Size: 573 378 4324
Fragments arranged by size:
4324 573 378
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2725 3486 4514 1540
Size: 275 186 724 761 1028 2301
Fragments arranged by size:
2301 1028 761 724 275 186
BsiHKAI G_wGCw'C
Cuts at: 3336 3421 4492 3336
Size: 85 1071 4119
Fragments arranged by size:
4119 1071 85
BsmI GAATG_Cn'
Cuts at: 2786 2879 2786
Size: 93 5182
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2420 2594 3336 3421 4492 1391
Size: 399 630 174 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 630 399 174 85
BspEI T'CCGG_A
Cuts at: 4938 4938
Size: 5275
BspHI T'CATG_A
Cuts at: 3164 4894 3164
Size: 1730 3545
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2070 2630 844
Size: 1226 560 3489
Fragments arranged by size:
3489 1226 560
BsrBI CCG'CTC
Cuts at: 1394 1823 3162 1394
Size: 429 1339 3507
Fragments arranged by size:
3507 1339 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3770 3944 65
Size: 1183 2522 174 1396
Fragments arranged by size:
2522 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3919 4106 1700
Size: 110 2109 187 2869
Fragments arranged by size:
2869 2109 187 110
BsrGI T'GTAC_A
Cuts at: 96 2397 96
Size: 2301 2974
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5275
BssSI C'ACGA_G
Cuts at: 3335 4347 3335
Size: 1012 4263
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5275
BstBI TT'CG_AA
Cuts at: 4079 4079
Size: 5275
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5275
BstYI r'GATC_y
Cuts at: 1972 2605 2972 3357 3374 4826 4912 4924 5270
Size: 633 367 385 17 1452 86 12 346
Cuts at: 5270 1972
Size: 1977
Fragments arranged by size:
1977 1452 633 385 367 346 86 17
12
Bsu36I CC'TnA_GG
Cuts at: 2255 2255
Size: 5275
BtgI C'CryG_G
Cuts at: 513 1065 2004 2220 2298 513
Size: 552 939 216 78 3490
Fragments arranged by size:
3490 939 552 216 78
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4536
Fragments arranged by size:
4536 683 56
BtsI GCAGTG_nn'
Cuts at: 2791 3585 3605 2791
Size: 794 20 4461
Fragments arranged by size:
4461 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5275
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5240
DraI TTT'AAA
Cuts at: 2579 2579
Size: 5275
DraIII CAC_nnn'GTG
Cuts at: 2025 2025
Size: 5275
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4282 809
Size: 507 2966 1802
Fragments arranged by size:
2966 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2439 2722 3611
Size: 54 1425 50 275 18 609 283 889
Cuts at: 3611 5240 8
Size: 1629 43
Fragments arranged by size:
1629 1425 889 609 283 275 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2722 1537
Size: 275 910 4090
Fragments arranged by size:
4090 910 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3205 1353
Size: 1852 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3862 4236 4382 387
Size: 1064 138 2273 374 146 1280
Fragments arranged by size:
2273 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3338 3935 4722 4990 1239
Size: 393 1706 597 787 268 1524
Fragments arranged by size:
1706 1524 787 597 393 268
EcoNI CCTnn'n_nnAGG
Cuts at: 2254 2254
Size: 5275
EcoO109I rG'GnC_Cy
Cuts at: 2304 2416 2417 2304
Size: 112 1 5162
Fragments arranged by size:
5162 112 1
EcoRI G'AATT_C
Cuts at: 2584 2962 2584
Size: 378 4897
FspI TGC'GCA
Cuts at: 3781 3781
Size: 5275
HaeII r_GCGC'y
Cuts at: 1678 2131 4422 1678
Size: 453 2291 2531
Fragments arranged by size:
2531 2291 453
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3916 3948 3990 4022 1373
Size: 32 135 32 2344 32 42 32 2626
Fragments arranged by size:
2626 2344 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2619 2865 669
Size: 988 281 681 246 3079
Fragments arranged by size:
3079 988 681 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5275
HpaI GTT'AAC
Cuts at: 2865 2865
Size: 5275
KpnI G_GTAC'C
Cuts at: 2600 2600
Size: 5275
MfeI C'AATT_G
Cuts at: 2874 2874
Size: 5275
MluI A'CGCG_T
Cuts at: 5151 5151
Size: 5275
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2178 4389 4573 1305
Size: 873 2211 184 2007
Fragments arranged by size:
2211 2007 873 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2441 10
Size: 54 1425 343 609 2844
Fragments arranged by size:
2844 1425 609 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3233 3592 3751 518
Size: 648 2067 359 159 2042
Fragments arranged by size:
2067 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2300 2691 3368 4516 4761 1606
Size: 694 391 677 1148 245 2120
Fragments arranged by size:
2120 1148 694 677 391 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3784
Fragments arranged by size:
3784 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5275
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5275
NotI GC'GGCC_GC
Cuts at: 2722 2722
Size: 5275
NspI r_CATG'y
Cuts at: 1132 1229 2633 4178 1132
Size: 97 1404 1545 2229
Fragments arranged by size:
2229 1545 1404 97
PasI CC'CwG_GG
Cuts at: 1348 2469 2512 1348
Size: 1121 43 4111
Fragments arranged by size:
4111 1121 43
PciI A'CATG_T
Cuts at: 4174 4174
Size: 5275
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5275
PfoI T'CCnGG_A
Cuts at: 2169 2169
Size: 5275
PmeI GTTT'AAAC
Cuts at: 2579 2579
Size: 5275
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3344 3376 4857 4889 3344
Size: 32 1481 32 3730
Fragments arranged by size:
3730 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2304 2304
Size: 5275
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5275
PsiI TTA'TAA
Cuts at: 2845 2845
Size: 5275
PspOMI G'GGCC_C
Cuts at: 2416 2416
Size: 5275
PspXI vC'TCGA_Gb
Cuts at: 2158 2158
Size: 5275
PstI C_TGCA'G
Cuts at: 830 2067 2295 2627 830
Size: 1237 228 332 3478
Fragments arranged by size:
3478 1237 332 228
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5275
SacII CC_GC'GG
Cuts at: 2301 2301
Size: 5275
SalI G'TCGA_C
Cuts at: 1936 2617 1936
Size: 681 4594
SanDI GG'GwC_CC
Cuts at: 2304 2304
Size: 5275
SbfI CC_TGCA'GG
Cuts at: 2067 2627 2067
Size: 560 4715
ScaI AGT'ACT
Cuts at: 3523 5235 3523
Size: 1712 3563
SfcI C'TryA_G
Cuts at: 826 1045 2063 2291 2317 2449 2623 3758 4439
Size: 219 1018 228 26 132 174 1135 681
Cuts at: 4439 4630 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1018 681 228 219 191 174
132 26
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5275
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5275
SmaI CCC'GGG
Cuts at: 1872 2602 1872
Size: 730 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2158 3379 4280 4542 820
Size: 197 926 215 1221 901 262 1553
Fragments arranged by size:
1553 1221 926 901 262 215 197
SnaBI TAC'GTA
Cuts at: 493 3066 493
Size: 2573 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5275
SphI G_CATG'C
Cuts at: 1132 2633 1132
Size: 1501 3774
SspI AAT'ATT
Cuts at: 5 52 3199 5
Size: 47 3147 2081
Fragments arranged by size:
3147 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5275
StyI C'CwwG_G
Cuts at: 513 1065 2004 2714 513
Size: 552 939 710 3074
Fragments arranged by size:
3074 939 710 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2387 3302 3319 3472 3657 4102 325
Size: 1255 807 915 17 153 185 445 1498
Fragments arranged by size:
1498 1255 915 807 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2397 3521 5233
Size: 274 80 33 51 1443 420 1124 1712
Cuts at: 5233 96
Size: 138
Fragments arranged by size:
1712 1443 1124 420 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5235 5267 5235
Size: 32 5243
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5275
XbaI T'CTAG_A
Cuts at: 2611 2611
Size: 5275
XhoI C'TCGA_G
Cuts at: 1943 2158 1943
Size: 215 5060
XmaI C'CCGG_G
Cuts at: 1870 2600 1870
Size: 730 4545
XmnI GAAnn'nnTTC
Cuts at: 3404 3404
Size: 5275
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3083 276
Size: 53 83 186 936 1549 2468
Fragments arranged by size:
2468 1549 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NspI
PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI
PsiI PspOMI PspXI PstI PvuI SacII SalI SanDI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
NsiI PacI PmlI PsrI PvuII RsrII SacI SapI
SexAI SfiI SfoI SrfI SwaI XcmI