(Circular) (Six-base) MAPSORT of: pFN21AB8264.seq Check: 4099 from: 1 to: 5335 pFN21AB8264 5335 bp With 182 enzymes: SgfI * February 27, 2009 14:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2560 2560 Size: 5335 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3145 278 Size: 53 83 186 936 1609 2468 Fragments arranged by size: 2468 1609 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2361 2678 1937 Size: 424 317 4594 Fragments arranged by size: 4594 424 317 Acc65I G'GTAC_C Cuts at: 2656 2656 Size: 5335 AclI AA'CG_TT Cuts at: 1563 3462 3835 1563 Size: 1899 373 3063 Fragments arranged by size: 3063 1899 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3398 4782 5050 1632 Size: 1766 1384 268 1917 Fragments arranged by size: 1917 1766 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5138 AflIII A'CryG_T Cuts at: 1203 4234 5211 1203 Size: 3031 977 1327 Fragments arranged by size: 3031 1327 977 AgeI A'CCGG_T Cuts at: 4166 4166 Size: 5335 AhdI GACnn_n'nnGTC Cuts at: 4064 4064 Size: 5335 AleI CACnn'nnGTG Cuts at: 1166 2073 1166 Size: 907 4428 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5335 AlwNI CAG_nnn'CTG Cuts at: 2113 2356 2395 2407 3078 4650 2113 Size: 243 39 12 671 1572 2798 Fragments arranged by size: 2798 1572 671 243 39 12 ApaLI G'TGCA_C Cuts at: 3392 4548 3392 Size: 1156 4179 ApoI r'AATT_y Cuts at: 1493 2644 2870 3022 5131 1493 Size: 1151 226 152 2109 1697 Fragments arranged by size: 2109 1697 1151 226 152 AseI AT'TA_AT Cuts at: 160 1032 3889 160 Size: 872 2857 1606 Fragments arranged by size: 2857 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5335 AvaI C'yCGr_G Cuts at: 1870 1943 2660 1870 Size: 73 717 4545 Fragments arranged by size: 4545 717 73 BamHI G'GATC_C Cuts at: 2520 2665 3032 2520 Size: 145 367 4823 Fragments arranged by size: 4823 367 145 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2656 4111 618 Size: 325 217 627 869 1455 1842 Fragments arranged by size: 1842 1455 869 627 325 217 BanII G_rGCy'C Cuts at: 1391 2244 2311 2654 1391 Size: 853 67 343 4072 Fragments arranged by size: 4072 853 343 67 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4364 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3526 3560 1903 Size: 34 1589 34 3678 Fragments arranged by size: 3678 1589 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3227 4437 3227 Size: 1210 4125 BclI T'GATC_A Cuts at: 1548 1548 Size: 5335 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3946 136 Size: 107 122 71 631 605 2274 1525 Fragments arranged by size: 2274 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5330 5330 Size: 5335 BlpI GC'TnA_GC Cuts at: 2752 2752 Size: 5335 Bme1580I G_kGCm'C Cuts at: 1790 3396 4552 1790 Size: 1606 1156 2573 Fragments arranged by size: 2573 1606 1156 BmgBI CAC'GTC Cuts at: 1318 2449 1318 Size: 1131 4204 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2586 3066 4024 Size: 416 53 83 399 24 1169 480 958 Cuts at: 4024 442 Size: 1753 Fragments arranged by size: 1753 1169 958 480 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5335 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2135 2405 3995 1239 Size: 896 270 1590 2579 Fragments arranged by size: 2579 1590 896 270 Bpu10I CC'TnA_GC Cuts at: 2442 2442 Size: 5335 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3460 4325 4623 3460 Size: 865 298 4172 Fragments arranged by size: 4172 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3998 882 Size: 617 2499 2219 Fragments arranged by size: 2499 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3126 493 Size: 711 1922 2702 Fragments arranged by size: 2702 1922 711 BsaBI GATnn'nnATC Cuts at: 5329 5329 Size: 5335 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3142 3524 4159 275 Size: 53 83 186 936 1609 382 635 1451 Fragments arranged by size: 1609 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3768 4166 4440 4587 4998 3768 Size: 398 274 147 411 4105 Fragments arranged by size: 4105 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2009 2039 1178 Size: 30 165 30 606 30 4474 Fragments arranged by size: 4474 606 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2030 2033 2132 2252 2306 2480 1186 Size: 844 3 99 120 54 174 4041 Fragments arranged by size: 4041 844 174 120 99 54 3 BseYI C'CCAG_C Cuts at: 4538 5034 4538 Size: 496 4839 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2342 2651 1163 Size: 573 606 309 3847 Fragments arranged by size: 3847 606 573 309 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2074 2162 2785 3546 4574 1540 Size: 275 186 73 88 623 761 1028 2301 Fragments arranged by size: 2301 1028 761 623 275 186 88 73 BsiHKAI G_wGCw'C Cuts at: 2430 2448 3396 3481 4552 2430 Size: 18 948 85 1071 3213 Fragments arranged by size: 3213 1071 948 85 18 BsmI GAATG_Cn' Cuts at: 2846 2939 2846 Size: 93 5242 BsmBI CGTCTCn'nnnn_ Cuts at: 2015 2591 2015 Size: 576 4759 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2244 2311 2430 2448 2654 3396 3481 Size: 399 454 67 119 18 206 742 85 Cuts at: 3481 4552 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 454 399 206 119 85 67 18 BspEI T'CCGG_A Cuts at: 4998 4998 Size: 5335 BspHI T'CATG_A Cuts at: 2454 3224 4954 2454 Size: 770 1730 2835 Fragments arranged by size: 2835 1730 770 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2560 2598 2623 2690 844 Size: 1716 38 25 67 3489 Fragments arranged by size: 3489 1716 67 38 25 BsrBI CCG'CTC Cuts at: 1394 1823 3222 1394 Size: 429 1399 3507 Fragments arranged by size: 3507 1399 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3830 4004 65 Size: 1183 2582 174 1396 Fragments arranged by size: 2582 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2028 2622 3979 4166 1700 Size: 110 218 594 1357 187 2869 Fragments arranged by size: 2869 1357 594 218 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5335 BssHII G'CGCG_C Cuts at: 1925 2543 1925 Size: 618 4717 BssSI C'ACGA_G Cuts at: 2429 3395 4407 2429 Size: 966 1012 3357 Fragments arranged by size: 3357 1012 966 BstAPI GCAn_nnn'nTGC Cuts at: 794 2395 2407 794 Size: 1601 12 3722 Fragments arranged by size: 3722 1601 12 BstBI TT'CG_AA Cuts at: 4139 4139 Size: 5335 BstEII G'GTnAC_C Cuts at: 1184 2086 1184 Size: 902 4433 BstXI CCAn_nnnn'nTGG Cuts at: 2075 2075 Size: 5335 BstYI r'GATC_y Cuts at: 1972 2520 2665 3032 3417 3434 4886 4972 4984 Size: 548 145 367 385 17 1452 86 12 Cuts at: 4984 5330 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 548 385 367 346 145 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2074 2162 513 Size: 552 939 70 88 3686 Fragments arranged by size: 3686 939 552 88 70 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4596 Fragments arranged by size: 4596 683 56 BtsI GCAGTG_nn' Cuts at: 2851 3645 3665 2851 Size: 794 20 4521 Fragments arranged by size: 4521 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5335 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5300 DraI TTT'AAA Cuts at: 2639 2639 Size: 5335 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4342 809 Size: 507 3026 1802 Fragments arranged by size: 3026 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2159 2782 3671 Size: 54 1425 50 275 18 329 623 889 Cuts at: 3671 5300 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 623 329 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2159 2782 1537 Size: 275 347 623 4090 Fragments arranged by size: 4090 623 347 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3265 1353 Size: 1912 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2332 3922 4296 4442 387 Size: 1064 138 743 1590 374 146 1280 Fragments arranged by size: 1590 1280 1064 743 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2135 2405 3398 3995 4782 5050 1239 Size: 393 503 270 993 597 787 268 1524 Fragments arranged by size: 1524 993 787 597 503 393 270 268 EcoO109I rG'GnC_Cy Cuts at: 2533 2533 Size: 5335 EcoRI G'AATT_C Cuts at: 2644 3022 2644 Size: 378 4957 FspI TGC'GCA Cuts at: 3841 3841 Size: 5335 HaeII r_GCGC'y Cuts at: 1678 4482 1678 Size: 2804 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2481 2513 3976 4008 4050 Size: 32 135 32 909 32 1463 32 42 Cuts at: 4050 4082 1373 Size: 32 2626 Fragments arranged by size: 2626 1463 909 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2362 2679 2925 669 Size: 988 281 424 317 246 3079 Fragments arranged by size: 3079 988 424 317 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5335 HpaI GTT'AAC Cuts at: 2925 2925 Size: 5335 KpnI G_GTAC'C Cuts at: 2660 2660 Size: 5335 MfeI C'AATT_G Cuts at: 2934 2934 Size: 5335 MluI A'CGCG_T Cuts at: 5211 5211 Size: 5335 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4449 4633 1305 Size: 3144 184 2007 Fragments arranged by size: 3144 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3513 Fragments arranged by size: 3513 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2073 2199 2267 2318 3293 3652 3811 Size: 648 907 126 68 51 975 359 159 Cuts at: 3811 518 Size: 2042 Fragments arranged by size: 2042 975 907 648 359 159 126 68 51 MspA1I CmG'CkG Cuts at: 1606 2062 2164 2512 2751 3428 4576 4821 1606 Size: 456 102 348 239 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 456 348 245 239 102 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3844 Fragments arranged by size: 3844 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5335 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5335 NotI GC'GGCC_GC Cuts at: 2159 2782 2159 Size: 623 4712 NspI r_CATG'y Cuts at: 1132 1229 2287 2693 4238 1132 Size: 97 1058 406 1545 2229 Fragments arranged by size: 2229 1545 1058 406 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5335 PciI A'CATG_T Cuts at: 4234 4234 Size: 5335 PflMI CCAn_nnn'nTGG Cuts at: 1108 2113 1108 Size: 1005 4330 PfoI T'CCnGG_A Cuts at: 2382 2382 Size: 5335 PmeI GTTT'AAAC Cuts at: 2639 2639 Size: 5335 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3404 3436 4917 4949 3404 Size: 32 1481 32 3790 Fragments arranged by size: 3790 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2533 2533 Size: 5335 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5335 PsiI TTA'TAA Cuts at: 2905 2905 Size: 5335 PstI C_TGCA'G Cuts at: 830 2400 2687 830 Size: 1570 287 3478 Fragments arranged by size: 3478 1570 287 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5335 PvuII CAG'CTG Cuts at: 2062 2512 2062 Size: 450 4885 SacII CC_GC'GG Cuts at: 2165 2165 Size: 5335 SalI G'TCGA_C Cuts at: 1936 2360 2677 1936 Size: 424 317 4594 Fragments arranged by size: 4594 424 317 SanDI GG'GwC_CC Cuts at: 2533 2533 Size: 5335 SbfI CC_TGCA'GG Cuts at: 2687 2687 Size: 5335 ScaI AGT'ACT Cuts at: 3583 5295 3583 Size: 1712 3623 SfcI C'TryA_G Cuts at: 826 1045 2396 2683 3818 4499 4690 826 Size: 219 1351 287 1135 681 191 1471 Fragments arranged by size: 1471 1351 1135 681 287 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5335 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5335 SmaI CCC'GGG Cuts at: 1872 2662 1872 Size: 790 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3439 4340 4602 820 Size: 197 926 1496 901 262 1553 Fragments arranged by size: 1553 1496 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3126 493 Size: 2633 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5335 SphI G_CATG'C Cuts at: 1132 2287 2693 1132 Size: 1155 406 3774 Fragments arranged by size: 3774 1155 406 SspI AAT'ATT Cuts at: 5 52 3259 5 Size: 47 3207 2081 Fragments arranged by size: 3207 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5335 StyI C'CwwG_G Cuts at: 513 1065 2004 2244 2460 2774 513 Size: 552 939 240 216 314 3074 Fragments arranged by size: 3074 939 552 314 240 216 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3362 3379 3532 3717 4162 325 Size: 1255 1782 17 153 185 445 1498 Fragments arranged by size: 1782 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2271 2376 3581 Size: 274 80 33 51 1443 294 105 1205 Cuts at: 3581 5293 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1205 294 274 138 105 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2548 2580 5295 5327 2548 Size: 32 2715 32 2556 Fragments arranged by size: 2715 2556 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5335 XbaI T'CTAG_A Cuts at: 2671 2671 Size: 5335 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2353 2380 2353 Size: 27 5308 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5335 XmaI C'CCGG_G Cuts at: 1870 2660 1870 Size: 790 4545 XmnI GAAnn'nnTTC Cuts at: 3464 3464 Size: 5335 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3143 276 Size: 53 83 186 936 1609 2468 Fragments arranged by size: 2468 1609 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SapI SexAI SfiI SfoI SrfI SwaI