Restriction map for
pFN21AB8262
##################################
# NO CUT ENZYMES ( 177 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspI AvaII
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmAI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrDI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI Cfr13I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EagI EarI EclXI Eco47III
Eco52I Eco81I EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HhaI Hin1I HinP1I
HincII HindII HindIII HpaI KasI
KpnI Ksp632I KspI MamI MfeI
MluI MluNI MroI MscI MslI
MunI MvnI NarI NciI NcoI
NdeI NheI NlaIII NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacII
SalI SapI Sau96I SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tsp509I
Tth111I Van91I XbaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AfaI 45 GTAC GT^AC
AhdI 313 GACATCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 313 GACATCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 187 GAGCTC G[AT]GC[AT]^C
AvaI 9 CTCGGG C^[CT]CG[GA]G
BfaI 85 CTAG C^TAG
BsiHKAI 187 GAGCTC G[AT]GC[AT]^C
BsmFI 321 GTCCC GGGAC(10/14)
BsoBI 9 CTCGGG C^[CT]CG[GA]G
BsrFI 386 GCCGGC [GA]^CCGG[CT]
Cfr10I 386 GCCGGC [GA]^CCGG[CT]
EaeI 38 TGGCCG [CT]^GGCC[GA]
Eam1105I 313 GACATCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 21 CCTGTCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 26 CCAAGG C^C[AT][AT]GG
HapII 387 CCGG C^CGG
HgaI 436 GCGTC GACGC(5/10)
HpaII 387 CCGG C^CGG
HphI 393 GGTGA GGTGA(8/7)
MaeI 85 CTAG C^TAG
MseI 560 TTAA T^TAA
MspA1I 124 CAGCGG C[AC]G^C[GT]G
MspI 387 CCGG C^CGG
MwoI 159 GCCCTTCGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NaeI 386 GCCGGC GCC^GGC
NgoMIV 386 GCCGGC G^CCGGC
PstI 219 CTGCAG CTGCA^G
RsaI 45 GTAC GT^AC
SacI 187 GAGCTC GAGCT^C
SfaNI 338 GCATC GCATC(5/9)
SfcI 219 CTGCAG C^T[GA][CT]AG
SmlI 190 CTCAAG C^T[CT][GA]AG
StyI 26 CCAAGG C^C[AT][AT]GG
Tru9I 560 TTAA T^TAA
XcmI 26 CCAAGGAGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AlwI 78 GATCC GGATC(4/5)
276 GGATC
BanII 6 GGGCTC G[GA]GC[CT]^C
187 GAGCTC
BbsI 376 GAAGAC GAAGAC(2/6)
226 GTCTTC
BpmI 539 CTCCAG CTGGAG(16/14)
469 CTGGAG
BpuAI 376 GAAGAC GAAGAC(2/6)
226 GTCTTC
BsaJI 26 CCAAGG C^C[ACGT][ACGT]GG
116 CCCAGG
BseRI 280 CTCCTC GAGGAG(10/8)
472 GAGGAG
BstYI 77 AGATCC [GA]^GATC[CT]
276 GGATCT
DpnII 78 GATC ^GATC
277 GATC
HaeIII 39 GGCC GG^CC
195 GGCC
MaeII 58 ACGT A^CGT
524 ACGT
MaeIII 141 GTCAC ^GT[ACGT]AC
296 GTGAC
MboI 78 GATC ^GATC
277 GATC
MboII 376 GAAGA GAAGA(8/7)
227 TCTTC
MflI 77 AGATCC [GA]^GATC[CT]
276 GGATCT
NdeII 78 GATC ^GATC
277 GATC
NlaIV 361 GGAACC GG[ACGT]^[ACGT]CC
496 GGAACC
PleI 136 GAGTC GAGTC(4/5)
475 GAGTC
Sau3AI 78 GATC ^GATC
277 GATC
TaqI 164 TCGA T^CGA
458 TCGA
TfiI 171 GATTC G^A[AT]TC
215 GAATC
Tsp45I 141 GTCAC ^GT[GC]AC
296 GTGAC
TspRI 285 CAGTG CA[GC]TG[ACGT][ACGT]^
294 CAGTG
TthHB8I 164 TCGA T^CGA
458 TCGA
XhoII 77 AGATCC [GA]^GATC[CT]
276 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AciI AluI BbvI BmyI BsiYI
BslI Bsp1286I BssKI BstF5I BstNI
Cac8I DdeI EcoRII Fnu4HI FokI
HinfI ItaI MnlI MvaI ScrFI
TseI