Restriction map for
pFN21AB8260
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AhdI AocI
Aor51HI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BalI BbrPI BbsI
BcgI BclI BcnI BfrI BglII
BlnI BpmI BpuAI BsaAI BsaBI
BsaI BsiEI BsiWI BsmAI BsmBI
BsmI BsoBI Bsp1407I BspDI BspHI
BspLU11I BspMI BsrBI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstZ17I
Bsu36I ClaI CpoI DraI DraIII
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI KspI
MamI MfeI MluI MluNI MscI
MseI MspA1I MunI NaeI NciI
NcoI NdeI NgoMIV NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SbfI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TaqI TfiI Tru9I
Tsp509I Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AccIII 110 TCCGGA T^CCGGA
AfaI 396 GTAC GT^AC
Alw44I 292 GTGCAC G^TGCAC
AlwNI 576 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 66 GGGCCC GGGCC^C
ApaLI 292 GTGCAC G^TGCAC
AvaII 498 GGACC G^G[AT]CC
BamHI 148 GGATCC G^GATCC
BanII 66 GGGCCC G[GA]GC[CT]^C
BbeI 547 GGCGCC GGCGC^C
BbvCI 351 CCTCAGC CCTCAGC(-5/-2)
BciVI 116 GTATCC GTATCC(6/5)
BfaI 439 CTAG C^TAG
BglI 26 GCCTTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 300 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 300 GCTGAGC GC^T[ACGT]AGC
BseAI 110 TCCGGA T^CCGGA
BspEI 110 TCCGGA T^CCGGA
BsrDI 84 CATTGC GCAATG(2/0)
BsrFI 500 ACCGGC [GA]^CCGG[CT]
BstEII 201 GGTCACC G^GT[ACGT]ACC
BstPI 201 GGTCACC G^GT[ACGT]ACC
BstXI 518 CCATGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 300 GCTGAGC GC^T[ACGT]AGC
Cfr10I 500 ACCGGC [GA]^CCGG[CT]
DraII 66 GGGCCCT [GA]G^G[ACGT]CC[CT]
DrdI 187 GACTCGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 507 CCACGG C^C[GA][CT]GG
EarI 56 CTCTTC CTCTTC(1/4)
EcoNI 420 CCTCAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 66 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 201 GGTCACC G^GT[ACGT]ACC
FauI 488 CCCGC CCCGC(4/6)
HaeII 547 GGCGCC [GA]GCGC^[CT]
HphI 203 TCACC GGTGA(8/7)
KasI 547 GGCGCC G^GCGCC
Ksp632I 56 CTCTTC CTCTTC(1/4)
MaeI 439 CTAG C^TAG
MaeII 161 ACGT A^CGT
MroI 110 TCCGGA T^CCGGA
NarI 547 GGCGCC GG^CGCC
NheI 438 GCTAGC G^CTAGC
RsaI 396 GTAC GT^AC
SapI 55 GCTCTTC GCTCTTC(1/4)
ScaI 395 AGTACT AGT^ACT
SfaNI 139 GATGC GCATC(5/9)
SfoI 547 GGCGCC GGC^GCC
SmlI 343 CTCAAG C^T[CT][GA]AG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcyI 415 GGCGTC G[GA]^CG[CT]C
547 GGCGCC
AspHI 292 GTGCAC G[AT]GC[AT]^C
571 GAGCAC
BsaHI 415 GGCGTC G[GA]^CG[CT]C
547 GGCGCC
BsaWI 60 TCCGGT [AT]^CCGG[AT]
110 TCCGGA
BseRI 349 CTCCTC GAGGAG(10/8)
427 GAGGAG
BsiHKAI 292 GTGCAC G[AT]GC[AT]^C
571 GAGCAC
BsmFI 328 GTCCC GGGAC(10/14)
367 GTCCC
BstF5I 309 GGATG GGATG(2/0)
528 GGATG
BstYI 148 GGATCC [GA]^GATC[CT]
251 AGATCC
DpnII 149 GATC ^GATC
252 GATC
FokI 309 GGATG GGATG(9/13)
528 GGATG
HgaI 416 GCGTC GACGC(5/10)
491 GCGTC
Hin1I 415 GGCGTC G[GA]^CG[CT]C
547 GGCGCC
MboI 149 GATC ^GATC
252 GATC
MboII 249 GAAGA GAAGA(8/7)
57 TCTTC
MflI 148 GGATCC [GA]^GATC[CT]
251 AGATCC
NdeII 149 GATC ^GATC
252 GATC
PstI 5 CTGCAG CTGCA^G
89 CTGCAG
Sau3AI 149 GATC ^GATC
252 GATC
SfcI 5 CTGCAG C^T[GA][CT]AG
89 CTGCAG
TspRI 290 CAGTG CA[GC]TG[ACGT][ACGT]^
456 CACTG
XhoII 148 GGATCC [GA]^GATC[CT]
251 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AccII AciI AluI AlwI BanI
BbvI BmyI BsaJI BsgI BsiYI
BslI Bsp1286I BsrI BssKI BstNI
BstUI Cac8I CfoI Cfr13I DdeI
EcoRII Fnu4HI HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MaeIII
MnlI MslI MspI MvaI MvnI
MwoI NlaIII NlaIV PleI Sau96I
ScrFI TseI Tsp45I