(Circular) (Six-base) MAPSORT of: pFN21AB8260.seq Check: 7070 from: 1 to: 5302 pFN21AB8260 5302 bp With 182 enzymes: SgfI * February 27, 2009 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3112 278 Size: 53 83 186 936 1576 2468 Fragments arranged by size: 2468 1576 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2645 1937 Size: 708 4594 Acc65I G'GTAC_C Cuts at: 2623 2623 Size: 5302 AclI AA'CG_TT Cuts at: 1563 3429 3802 1563 Size: 1866 373 3063 Fragments arranged by size: 3063 1866 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2273 2397 3365 4749 5017 1632 Size: 641 124 968 1384 268 1917 Fragments arranged by size: 1917 1384 968 641 268 124 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5105 AflIII A'CryG_T Cuts at: 1203 4201 5178 1203 Size: 2998 977 1327 Fragments arranged by size: 2998 1327 977 AgeI A'CCGG_T Cuts at: 4133 4133 Size: 5302 AhdI GACnn_n'nnGTC Cuts at: 4031 4031 Size: 5302 AleI CACnn'nnGTG Cuts at: 1166 2471 1166 Size: 1305 3997 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2489 2540 1184 Size: 1305 51 3946 Fragments arranged by size: 3946 1305 51 AlwNI CAG_nnn'CTG Cuts at: 2586 3045 4617 2586 Size: 459 1572 3271 Fragments arranged by size: 3271 1572 459 ApaI G_GGCC'C Cuts at: 2075 2075 Size: 5302 ApaLI G'TGCA_C Cuts at: 2297 3359 4515 2297 Size: 1062 1156 3084 Fragments arranged by size: 3084 1156 1062 ApoI r'AATT_y Cuts at: 1493 2611 2837 2989 5098 1493 Size: 1118 226 152 2109 1697 Fragments arranged by size: 2109 1697 1118 226 152 AseI AT'TA_AT Cuts at: 160 1032 3856 160 Size: 872 2824 1606 Fragments arranged by size: 2824 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5302 AvaI C'yCGr_G Cuts at: 1870 1943 2627 1870 Size: 73 684 4545 Fragments arranged by size: 4545 684 73 BamHI G'GATC_C Cuts at: 2153 2632 2999 2153 Size: 479 367 4456 Fragments arranged by size: 4456 479 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2326 2365 2465 2508 2552 Size: 325 217 627 539 39 100 43 44 Cuts at: 2552 2623 4078 618 Size: 71 1455 1842 Fragments arranged by size: 1842 1455 627 539 325 217 100 71 44 43 39 BanII G_rGCy'C Cuts at: 1391 2075 2621 1391 Size: 684 546 4072 Fragments arranged by size: 4072 684 546 BbeI G_GCGC'C Cuts at: 2556 2556 Size: 5302 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4331 BbvCI CC'TCA_GC Cuts at: 2357 2357 Size: 5302 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3493 3527 1903 Size: 34 1556 34 3678 Fragments arranged by size: 3678 1556 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2132 3194 4404 2132 Size: 1062 1210 3030 Fragments arranged by size: 3030 1210 1062 BclI T'GATC_A Cuts at: 1548 1548 Size: 5302 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2037 3913 136 Size: 107 122 71 631 605 365 1876 1525 Fragments arranged by size: 1876 1525 631 605 365 122 107 71 BglII A'GATC_T Cuts at: 5297 5297 Size: 5302 BlpI GC'TnA_GC Cuts at: 2306 2719 2306 Size: 413 4889 Bme1580I G_kGCm'C Cuts at: 1790 2075 2301 2370 3363 4519 1790 Size: 285 226 69 993 1156 2573 Fragments arranged by size: 2573 1156 993 285 226 69 BmgBI CAC'GTC Cuts at: 1318 2167 1318 Size: 849 4453 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2288 3033 3991 Size: 416 53 83 399 24 871 745 958 Cuts at: 3991 442 Size: 1753 Fragments arranged by size: 1753 958 871 745 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2447 1056 Size: 1391 3911 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3962 1239 Size: 2723 2579 Bpu10I CC'TnA_GC Cuts at: 2357 2357 Size: 5302 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2333 3427 4292 4590 2333 Size: 1094 865 298 3045 Fragments arranged by size: 3045 1094 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3965 882 Size: 617 2466 2219 Fragments arranged by size: 2466 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3093 493 Size: 711 1889 2702 Fragments arranged by size: 2702 1889 711 BsaBI GATnn'nnATC Cuts at: 5296 5296 Size: 5302 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2421 2553 3109 3491 Size: 53 83 186 936 888 132 556 382 Cuts at: 3491 4126 275 Size: 635 1451 Fragments arranged by size: 1451 936 888 635 556 382 186 132 83 53 BsaWI w'CCGG_w Cuts at: 2065 2115 3735 4133 4407 4554 4965 2065 Size: 50 1620 398 274 147 411 2402 Fragments arranged by size: 2402 1620 411 398 274 147 50 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5077 Fragments arranged by size: 5077 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2345 2447 1186 Size: 1159 102 4041 Fragments arranged by size: 4041 1159 102 BseYI C'CCAG_C Cuts at: 2336 4505 5001 2336 Size: 2169 496 2637 Fragments arranged by size: 2637 2169 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2246 2327 2507 1163 Size: 573 510 81 180 3958 Fragments arranged by size: 3958 573 510 180 81 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2752 3513 4541 1540 Size: 275 186 751 761 1028 2301 Fragments arranged by size: 2301 1028 761 751 275 186 BsiHKAI G_wGCw'C Cuts at: 2301 2580 3363 3448 4519 2301 Size: 279 783 85 1071 3084 Fragments arranged by size: 3084 1071 783 279 85 BsmI GAATG_Cn' Cuts at: 2813 2906 2813 Size: 93 5209 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2075 2301 2370 2580 2621 3363 3448 Size: 399 285 226 69 210 41 742 85 Cuts at: 3448 4519 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 285 226 210 85 69 41 BspEI T'CCGG_A Cuts at: 2115 4965 2115 Size: 2850 2452 BspHI T'CATG_A Cuts at: 3191 4921 3191 Size: 1730 3572 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2657 844 Size: 1813 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3189 1394 Size: 429 1366 3507 Fragments arranged by size: 3507 1366 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2088 3797 3971 65 Size: 1183 840 1709 174 1396 Fragments arranged by size: 1709 1396 1183 840 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2505 3946 4133 1700 Size: 110 695 1441 187 2869 Fragments arranged by size: 2869 1441 695 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5302 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5302 BssSI C'ACGA_G Cuts at: 3362 4374 3362 Size: 1012 4290 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5302 BstBI TT'CG_AA Cuts at: 4106 4106 Size: 5302 BstEII G'GTnAC_C Cuts at: 1184 2206 1184 Size: 1022 4280 BstXI CCAn_nnnn'nTGG Cuts at: 2530 2530 Size: 5302 BstYI r'GATC_y Cuts at: 1972 2153 2256 2632 2999 3384 3401 4853 4939 Size: 181 103 376 367 385 17 1452 86 Cuts at: 4939 4951 5297 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 385 376 367 346 181 103 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2512 513 Size: 552 939 508 3303 Fragments arranged by size: 3303 939 552 508 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4563 Fragments arranged by size: 4563 683 56 BtsI GCAGTG_nn' Cuts at: 2818 3612 3632 2818 Size: 794 20 4488 Fragments arranged by size: 4488 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5302 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5267 DraI TTT'AAA Cuts at: 2606 2606 Size: 5302 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2198 4309 809 Size: 507 882 2111 1802 Fragments arranged by size: 2111 1802 882 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2749 3638 5267 Size: 54 1425 50 275 18 919 889 1629 Cuts at: 5267 8 Size: 43 Fragments arranged by size: 1629 1425 919 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2749 1537 Size: 275 937 4090 Fragments arranged by size: 4090 937 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2067 3232 1353 Size: 714 1165 3423 Fragments arranged by size: 3423 1165 714 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3889 4263 4409 387 Size: 1064 138 2300 374 146 1280 Fragments arranged by size: 2300 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2273 2397 3365 3962 4749 5017 1239 Size: 393 641 124 968 597 787 268 1524 Fragments arranged by size: 1524 968 787 641 597 393 268 124 EcoNI CCTnn'n_nnAGG Cuts at: 2429 2429 Size: 5302 EcoO109I rG'GnC_Cy Cuts at: 2072 2072 Size: 5302 EcoRI G'AATT_C Cuts at: 2611 2989 2611 Size: 378 4924 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2263 2263 Size: 5302 FspI TGC'GCA Cuts at: 3808 3808 Size: 5302 HaeII r_GCGC'y Cuts at: 1678 2556 4449 1678 Size: 878 1893 2531 Fragments arranged by size: 2531 1893 878 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3943 3975 4017 4049 1373 Size: 32 135 32 2371 32 42 32 2626 Fragments arranged by size: 2626 2371 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2646 2892 669 Size: 988 281 708 246 3079 Fragments arranged by size: 3079 988 708 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5302 HpaI GTT'AAC Cuts at: 2892 2892 Size: 5302 KasI G'GCGC_C Cuts at: 2552 2552 Size: 5302 KpnI G_GTAC'C Cuts at: 2627 2627 Size: 5302 MfeI C'AATT_G Cuts at: 2901 2901 Size: 5302 MluI A'CGCG_T Cuts at: 5178 5178 Size: 5302 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4416 4600 1305 Size: 3111 184 2007 Fragments arranged by size: 3111 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3480 Fragments arranged by size: 3480 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2175 2268 2471 2522 3260 3619 3778 Size: 648 1009 93 203 51 738 359 159 Cuts at: 3778 518 Size: 2042 Fragments arranged by size: 2042 1009 738 648 359 203 159 93 51 MspA1I CmG'CkG Cuts at: 1606 2718 3395 4543 4788 1606 Size: 1112 677 1148 245 2120 Fragments arranged by size: 2120 1148 1112 677 245 NarI GG'CG_CC Cuts at: 2553 2553 Size: 5302 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3811 Fragments arranged by size: 3811 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5302 NheI G'CTAG_C Cuts at: 1052 2443 1052 Size: 1391 3911 NotI GC'GGCC_GC Cuts at: 2749 2749 Size: 5302 NspI r_CATG'y Cuts at: 1132 1229 2660 4205 1132 Size: 97 1431 1545 2229 Fragments arranged by size: 2229 1545 1431 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5302 PciI A'CATG_T Cuts at: 4201 4201 Size: 5302 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5302 PmeI GTTT'AAAC Cuts at: 2606 2606 Size: 5302 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3371 3403 4884 4916 3371 Size: 32 1481 32 3757 Fragments arranged by size: 3757 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5302 PsiI TTA'TAA Cuts at: 2872 2872 Size: 5302 PspOMI G'GGCC_C Cuts at: 2071 2071 Size: 5302 PstI C_TGCA'G Cuts at: 830 2014 2098 2654 830 Size: 1184 84 556 3478 Fragments arranged by size: 3478 1184 556 84 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5302 SalI G'TCGA_C Cuts at: 1936 2644 1936 Size: 708 4594 SapI GCTCTTCn'nnn_ Cuts at: 2067 2067 Size: 5302 SbfI CC_TGCA'GG Cuts at: 2654 2654 Size: 5302 ScaI AGT'ACT Cuts at: 2402 3550 5262 2402 Size: 1148 1712 2442 Fragments arranged by size: 2442 1712 1148 SfcI C'TryA_G Cuts at: 826 1045 2010 2094 2650 3785 4466 4657 826 Size: 219 965 84 556 1135 681 191 1471 Fragments arranged by size: 1471 1135 965 681 556 219 191 84 SfoI GGC'GCC Cuts at: 2554 2554 Size: 5302 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5302 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5302 SmaI CCC'GGG Cuts at: 1872 2629 1872 Size: 757 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2348 3406 4307 4569 820 Size: 197 926 405 1058 901 262 1553 Fragments arranged by size: 1553 1058 926 901 405 262 197 SnaBI TAC'GTA Cuts at: 493 3093 493 Size: 2600 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5302 SphI G_CATG'C Cuts at: 1132 2660 1132 Size: 1528 3774 SspI AAT'ATT Cuts at: 5 52 3226 5 Size: 47 3174 2081 Fragments arranged by size: 3174 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5302 StyI C'CwwG_G Cuts at: 513 1065 2004 2741 513 Size: 552 939 737 3074 Fragments arranged by size: 3074 939 737 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2225 3329 3346 3499 3684 4129 325 Size: 1255 645 1104 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1104 645 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2400 3548 5260 Size: 274 80 33 51 1443 423 1148 1712 Cuts at: 5260 96 Size: 138 Fragments arranged by size: 1712 1443 1148 423 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5262 5294 5262 Size: 32 5270 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5302 XbaI T'CTAG_A Cuts at: 2638 2638 Size: 5302 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5302 XmaI C'CCGG_G Cuts at: 1870 2627 1870 Size: 757 4545 XmnI GAAnn'nnTTC Cuts at: 3431 3431 Size: 5302 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3110 276 Size: 53 83 186 936 1576 2468 Fragments arranged by size: 2468 1576 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI XcmI