Restriction map for
pFN21AB8260
(Linear) (Six-base) MAPSORT of: KIBB8260.seq Check: 2180 from: 1 to: 597
KIBB8260.orf
With 182 enzymes: SgfI *
March 3, 2008 11:04 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 392 597
Size: 268 124 205
Fragments arranged by size:
268 205 124
AleI CACnn'nnGTG
Cuts at: 0 466 597
Size: 466 131
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 484 535 597
Size: 484 51 62
Fragments arranged by size:
484 62 51
AlwNI CAG_nnn'CTG
Cuts at: 0 581 597
Size: 581 16
ApaI G_GGCC'C
Cuts at: 0 70 597
Size: 70 527
ApaLI G'TGCA_C
Cuts at: 0 292 597
Size: 292 305
BamHI G'GATC_C
Cuts at: 0 148 597
Size: 148 449
BanI G'GyrC_C
Cuts at: 0 321 360 460 503 547 597
Size: 321 39 100 43 44 50
Fragments arranged by size:
321 100 50 44 43 39
BanII G_rGCy'C
Cuts at: 0 70 597
Size: 70 527
BbeI G_GCGC'C
Cuts at: 0 551 597
Size: 551 46
BbvCI CC'TCA_GC
Cuts at: 0 352 597
Size: 352 245
BciVI GTATCCnnnnn_n'
Cuts at: 0 127 597
Size: 127 470
BglI GCCn_nnn'nGGC
Cuts at: 0 32 597
Size: 32 565
BlpI GC'TnA_GC
Cuts at: 0 301 597
Size: 301 296
Bme1580I G_kGCm'C
Cuts at: 0 70 296 365 597
Size: 70 226 69 232
Fragments arranged by size:
232 226 70 69
BmgBI CAC'GTC
Cuts at: 0 162 597
Size: 162 435
BmrI ACTGGGnnnn_n'
Cuts at: 0 283 597
Size: 283 314
BmtI G_CTAG'C
Cuts at: 0 442 597
Size: 442 155
Bpu10I CC'TnA_GC
Cuts at: 0 352 597
Size: 352 245
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 328 597
Size: 328 269
BsaHI Gr'CG_yC
Cuts at: 0 416 548 597
Size: 416 132 49
Fragments arranged by size:
416 132 49
BsaWI w'CCGG_w
Cuts at: 0 60 110 597
Size: 60 50 487
Fragments arranged by size:
487 60 50
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 340 442 597
Size: 340 102 155
Fragments arranged by size:
340 155 102
BseYI C'CCAG_C
Cuts at: 0 331 597
Size: 331 266
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 322 502 597
Size: 241 81 180 95
Fragments arranged by size:
241 180 95 81
BsiHKAI G_wGCw'C
Cuts at: 0 296 575 597
Size: 296 279 22
Fragments arranged by size:
296 279 22
Bsp1286I G_dGCh'C
Cuts at: 0 70 296 365 575 597
Size: 70 226 69 210 22
Fragments arranged by size:
226 210 70 69 22
BspEI T'CCGG_A
Cuts at: 0 110 597
Size: 110 487
BsrDI GCAATG_nn'
Cuts at: 0 83 597
Size: 83 514
BsrFI r'CCGG_y
Cuts at: 0 500 597
Size: 500 97
BstEII G'GTnAC_C
Cuts at: 0 201 597
Size: 201 396
BstXI CCAn_nnnn'nTGG
Cuts at: 0 525 597
Size: 525 72
BstYI r'GATC_y
Cuts at: 0 148 251 597
Size: 148 103 346
Fragments arranged by size:
346 148 103
BtgI C'CryG_G
Cuts at: 0 507 597
Size: 507 90
DrdI GACnn_nn'nnGTC
Cuts at: 0 193 597
Size: 193 404
EarI CTCTTCn'nnn_
Cuts at: 0 62 597
Size: 62 535
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 268 392 597
Size: 268 124 205
Fragments arranged by size:
268 205 124
EcoNI CCTnn'n_nnAGG
Cuts at: 0 424 597
Size: 424 173
EcoO109I rG'GnC_Cy
Cuts at: 0 67 597
Size: 67 530
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 258 597
Size: 258 339
HaeII r_GCGC'y
Cuts at: 0 551 597
Size: 551 46
KasI G'GCGC_C
Cuts at: 0 547 597
Size: 547 50
MslI CAynn'nnrTG
Cuts at: 0 170 263 466 517 597
Size: 170 93 203 51 80
Fragments arranged by size:
203 170 93 80 51
NarI GG'CG_CC
Cuts at: 0 548 597
Size: 548 49
NheI G'CTAG_C
Cuts at: 0 438 597
Size: 438 159
PspOMI G'GGCC_C
Cuts at: 0 66 597
Size: 66 531
PstI C_TGCA'G
Cuts at: 0 9 93 597
Size: 9 84 504
Fragments arranged by size:
504 84 9
SapI GCTCTTCn'nnn_
Cuts at: 0 62 597
Size: 62 535
ScaI AGT'ACT
Cuts at: 0 397 597
Size: 397 200
SfcI C'TryA_G
Cuts at: 0 5 89 597
Size: 5 84 508
Fragments arranged by size:
508 84 5
SfoI GGC'GCC
Cuts at: 0 549 597
Size: 549 48
SmlI C'TyrA_G
Cuts at: 0 343 597
Size: 343 254
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 220 597
Size: 220 377
TatI w'GTAC_w
Cuts at: 0 395 597
Size: 395 202
Enzymes that do cut:
AcuI AleI AlfI AlwNI ApaI ApaLI BamHI BanI
BanII BbeI BbvCI BciVI BglI BlpI Bme1580I BmgBI
BmrI BmtI Bpu10I BpuEI BsaHI BsaWI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspEI BsrDI BsrFI BstEII BstXI
BstYI BtgI DrdI EarI Eco57MI EcoNI EcoO109I FalI
HaeII KasI MslI NarI NheI PspOMI PstI SapI
ScaI SfcI SfoI SmlI TaqII TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AloI ApoI AscI AseI AsiSI AvaI
AvrII BaeI BbsI BcgI BclI BfrBI BglII BplI
BpmI BsaI BsaAI BsaBI BsaXI BsiEI BsiWI BsmI
BsmBI BspHI BspMI BsrBI BsrGI BssHII BssSI BstAPI
BstBI BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII EaeI EagI EciI EcoICRI EcoRI EcoRV FseI
FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MspA1I NaeI NcoI NdeI NgoMIV
NotI NruI NsiI NspI PacI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SbfI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI StuI StyI SwaI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI