(Circular) (Six-base) MAPSORT of: pFN21AB8256.seq Check: 3347 from: 1 to: 5770 pFN21AB8256 5770 bp With 182 enzymes: SgfI * February 27, 2009 14:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2514 2514 Size: 5770 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3580 278 Size: 53 83 186 936 2044 2468 Fragments arranged by size: 2468 2044 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2052 2837 3113 1937 Size: 115 785 276 4594 Fragments arranged by size: 4594 785 276 115 Acc65I G'GTAC_C Cuts at: 3091 3091 Size: 5770 AclI AA'CG_TT Cuts at: 1563 3897 4270 1563 Size: 2334 373 3063 Fragments arranged by size: 3063 2334 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2627 3833 5217 5485 1632 Size: 995 1206 1384 268 1917 Fragments arranged by size: 1917 1384 1206 995 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5573 AflIII A'CryG_T Cuts at: 1203 4669 5646 1203 Size: 3466 977 1327 Fragments arranged by size: 3466 1327 977 AgeI A'CCGG_T Cuts at: 3000 4601 3000 Size: 1601 4169 AhdI GACnn_n'nnGTC Cuts at: 2167 4499 2167 Size: 2332 3438 AleI CACnn'nnGTG Cuts at: 1166 2528 1166 Size: 1362 4408 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2231 1184 Size: 1047 4723 AlwNI CAG_nnn'CTG Cuts at: 2080 2770 3513 5085 2080 Size: 690 743 1572 2765 Fragments arranged by size: 2765 1572 743 690 ApaLI G'TGCA_C Cuts at: 3827 4983 3827 Size: 1156 4614 ApoI r'AATT_y Cuts at: 1493 2282 3079 3305 3457 5566 1493 Size: 789 797 226 152 2109 1697 Fragments arranged by size: 2109 1697 797 789 226 152 AseI AT'TA_AT Cuts at: 160 1032 4324 160 Size: 872 3292 1606 Fragments arranged by size: 3292 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5770 AvaI C'yCGr_G Cuts at: 1870 1943 2640 3095 1870 Size: 73 697 455 4545 Fragments arranged by size: 4545 697 455 73 BamHI G'GATC_C Cuts at: 3100 3467 3100 Size: 367 5403 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2502 2644 3091 4546 618 Size: 325 217 627 715 142 447 1455 1842 Fragments arranged by size: 1842 1455 715 627 447 325 217 142 BanII G_rGCy'C Cuts at: 1391 2858 2938 3089 1391 Size: 1467 80 151 4072 Fragments arranged by size: 4072 1467 151 80 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3031 3052 928 Size: 971 1132 21 3646 Fragments arranged by size: 3646 1132 971 21 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2098 2132 3961 3995 1903 Size: 34 161 34 1829 34 3678 Fragments arranged by size: 3678 1829 161 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3662 4872 3662 Size: 1210 4560 BclI T'GATC_A Cuts at: 1548 1548 Size: 5770 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2496 4381 136 Size: 107 122 71 631 605 824 1885 1525 Fragments arranged by size: 1885 1525 824 631 605 122 107 71 BglII A'GATC_T Cuts at: 5765 5765 Size: 5770 BlpI GC'TnA_GC Cuts at: 2457 3187 2457 Size: 730 5040 Bme1580I G_kGCm'C Cuts at: 1790 2647 3831 4987 1790 Size: 857 1184 1156 2573 Fragments arranged by size: 2573 1184 1156 857 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5770 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2129 3501 4459 Size: 416 53 83 399 24 712 1372 958 Cuts at: 4459 442 Size: 1753 Fragments arranged by size: 1753 1372 958 712 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5770 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2653 2872 2653 Size: 219 5551 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2360 2447 4430 1239 Size: 1121 87 1983 2579 Fragments arranged by size: 2579 1983 1121 87 Bpu10I CC'TnA_GC Cuts at: 2318 2318 Size: 5770 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2957 3895 4760 5058 2957 Size: 938 865 298 3669 Fragments arranged by size: 3669 938 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2066 2539 4433 882 Size: 617 567 473 1894 2219 Fragments arranged by size: 2219 1894 617 567 473 BsaAI yAC'GTr Cuts at: 493 1204 3561 493 Size: 711 2357 2702 Fragments arranged by size: 2702 2357 711 BsaBI GATnn'nnATC Cuts at: 5764 5764 Size: 5770 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3577 3959 4594 275 Size: 53 83 186 936 2044 382 635 1451 Fragments arranged by size: 2044 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3000 4203 4601 4875 5022 5433 3000 Size: 1203 398 274 147 411 3337 Fragments arranged by size: 3337 1203 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2183 2213 1178 Size: 30 165 30 780 30 4735 Fragments arranged by size: 4735 780 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2632 1186 Size: 1446 4324 BseYI C'CCAG_C Cuts at: 2937 4973 5469 2937 Size: 2036 496 3238 Fragments arranged by size: 3238 2036 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5197 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3220 3981 5009 1540 Size: 275 186 1219 761 1028 2301 Fragments arranged by size: 2301 1219 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3831 3916 4987 3831 Size: 85 1071 4614 Fragments arranged by size: 4614 1071 85 BsmI GAATG_Cn' Cuts at: 3281 3374 3281 Size: 93 5677 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2647 2858 2938 3089 3831 3916 4987 Size: 399 857 211 80 151 742 85 1071 Cuts at: 4987 1391 Size: 2174 Fragments arranged by size: 2174 1071 857 742 399 211 151 85 80 BspEI T'CCGG_A Cuts at: 5433 5433 Size: 5770 BspHI T'CATG_A Cuts at: 3659 5389 3659 Size: 1730 4040 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2514 3125 844 Size: 1670 611 3489 Fragments arranged by size: 3489 1670 611 BsrBI CCG'CTC Cuts at: 1394 1823 3657 1394 Size: 429 1834 3507 Fragments arranged by size: 3507 1834 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4265 4439 65 Size: 1183 3017 174 1396 Fragments arranged by size: 3017 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2499 3000 4414 4601 1700 Size: 110 689 501 1414 187 2869 Fragments arranged by size: 2869 1414 689 501 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5770 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5770 BssSI C'ACGA_G Cuts at: 3830 4842 3830 Size: 1012 4758 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5770 BstBI TT'CG_AA Cuts at: 4574 4574 Size: 5770 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5770 BstYI r'GATC_y Cuts at: 1972 2967 3100 3467 3852 3869 5321 5407 5419 Size: 995 133 367 385 17 1452 86 12 Cuts at: 5419 5765 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 995 385 367 346 133 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2012 513 Size: 552 947 4271 Fragments arranged by size: 4271 947 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2101 487 Size: 683 56 875 4156 Fragments arranged by size: 4156 875 683 56 BtsI GCAGTG_nn' Cuts at: 2284 2397 3286 4080 4100 2284 Size: 113 889 794 20 3954 Fragments arranged by size: 3954 889 794 113 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5770 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5735 DraI TTT'AAA Cuts at: 3074 3074 Size: 5770 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4777 809 Size: 507 3461 1802 Fragments arranged by size: 3461 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2295 2497 3217 Size: 54 1425 50 275 18 465 202 720 Cuts at: 3217 4106 5735 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 720 465 275 202 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3217 1537 Size: 275 1405 4090 Fragments arranged by size: 4090 1405 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2397 3700 1353 Size: 1044 1303 3423 Fragments arranged by size: 3423 1303 1044 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4357 4731 4877 387 Size: 1064 138 2768 374 146 1280 Fragments arranged by size: 2768 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2360 2447 2627 3833 4430 5217 5485 Size: 393 728 87 180 1206 597 787 268 Cuts at: 5485 1239 Size: 1524 Fragments arranged by size: 1524 1206 787 728 597 393 268 180 87 EcoO109I rG'GnC_Cy Cuts at: 2077 2077 Size: 5770 EcoRI G'AATT_C Cuts at: 2282 3079 3457 2282 Size: 797 378 4595 Fragments arranged by size: 4595 797 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2392 2392 Size: 5770 FspI TGC'GCA Cuts at: 4276 4276 Size: 5770 HaeII r_GCGC'y Cuts at: 1678 4917 1678 Size: 3239 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2153 2183 2185 2215 2561 Size: 32 135 32 581 30 2 30 346 Cuts at: 2561 2593 2737 2769 4411 4443 4485 4517 1373 Size: 32 144 32 1642 32 42 32 2626 Fragments arranged by size: 2626 1642 581 346 144 135 42 32 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 669 1657 1938 2838 3114 3360 669 Size: 988 281 900 276 246 3079 Fragments arranged by size: 3079 988 900 281 276 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5770 HpaI GTT'AAC Cuts at: 3360 3360 Size: 5770 KpnI G_GTAC'C Cuts at: 3095 3095 Size: 5770 MfeI C'AATT_G Cuts at: 2841 3369 2841 Size: 528 5242 MluI A'CGCG_T Cuts at: 5646 5646 Size: 5770 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2293 2544 3021 4884 5068 1305 Size: 988 251 477 1863 184 2007 Fragments arranged by size: 2007 1863 988 477 251 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2297 10 Size: 54 1425 343 465 3483 Fragments arranged by size: 3483 1425 465 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2291 2528 2741 3053 3728 4087 4246 Size: 648 1125 237 213 312 675 359 159 Cuts at: 4246 518 Size: 2042 Fragments arranged by size: 2042 1125 675 648 359 312 237 213 159 MspA1I CmG'CkG Cuts at: 1606 2014 2770 2941 3186 3863 5011 5256 1606 Size: 408 756 171 245 677 1148 245 2120 Fragments arranged by size: 2120 1148 756 677 408 245 245 171 NaeI GCC'GGC Cuts at: 2501 2501 Size: 5770 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5218 NdeI CA'TA_TG Cuts at: 387 387 Size: 5770 NgoMIV G'CCGG_C Cuts at: 2499 2499 Size: 5770 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5770 NotI GC'GGCC_GC Cuts at: 3217 3217 Size: 5770 NspI r_CATG'y Cuts at: 1132 1229 3128 4673 1132 Size: 97 1899 1545 2229 Fragments arranged by size: 2229 1899 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5770 PciI A'CATG_T Cuts at: 4669 4669 Size: 5770 PflMI CCAn_nnn'nTGG Cuts at: 1108 3003 1108 Size: 1895 3875 PfoI T'CCnGG_A Cuts at: 2463 2463 Size: 5770 PmeI GTTT'AAAC Cuts at: 3074 3074 Size: 5770 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3839 3871 5352 5384 3839 Size: 32 1481 32 4225 Fragments arranged by size: 4225 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2077 2077 Size: 5770 PshAI GACnn'nnGTC Cuts at: 1613 2075 2144 1613 Size: 462 69 5239 Fragments arranged by size: 5239 462 69 PsiI TTA'TAA Cuts at: 3340 3340 Size: 5770 PstI C_TGCA'G Cuts at: 830 2220 2511 2775 3122 830 Size: 1390 291 264 347 3478 Fragments arranged by size: 3478 1390 347 291 264 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5770 PvuII CAG'CTG Cuts at: 2770 2941 2770 Size: 171 5599 SacII CC_GC'GG Cuts at: 2015 2015 Size: 5770 SalI G'TCGA_C Cuts at: 1936 2836 3112 1936 Size: 900 276 4594 Fragments arranged by size: 4594 900 276 SapI GCTCTTCn'nnn_ Cuts at: 2397 2397 Size: 5770 SbfI CC_TGCA'GG Cuts at: 3122 3122 Size: 5770 ScaI AGT'ACT Cuts at: 2978 4018 5730 2978 Size: 1040 1712 3018 Fragments arranged by size: 3018 1712 1040 SfcI C'TryA_G Cuts at: 826 1045 2216 2507 2771 3118 4253 4934 5125 Size: 219 1171 291 264 347 1135 681 191 Cuts at: 5125 826 Size: 1471 Fragments arranged by size: 1471 1171 1135 681 347 291 264 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5770 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5770 SmaI CCC'GGG Cuts at: 1872 2642 3097 1872 Size: 770 455 4545 Fragments arranged by size: 4545 770 455 SmlI C'TyrA_G Cuts at: 820 1017 1943 2972 3874 4775 5037 820 Size: 197 926 1029 902 901 262 1553 Fragments arranged by size: 1553 1029 926 902 901 262 197 SnaBI TAC'GTA Cuts at: 493 3561 493 Size: 3068 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5770 SphI G_CATG'C Cuts at: 1132 3128 1132 Size: 1996 3774 SspI AAT'ATT Cuts at: 5 52 3694 5 Size: 47 3642 2081 Fragments arranged by size: 3642 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5770 StyI C'CwwG_G Cuts at: 513 1065 2481 2724 3209 513 Size: 552 1416 243 485 3074 Fragments arranged by size: 3074 1416 552 485 243 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3797 3814 3967 4152 4597 325 Size: 1255 2217 17 153 185 445 1498 Fragments arranged by size: 2217 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2976 4016 5728 Size: 274 80 33 51 1443 999 1040 1712 Cuts at: 5728 96 Size: 138 Fragments arranged by size: 1712 1443 1040 999 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5730 5762 5730 Size: 32 5738 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5770 XbaI T'CTAG_A Cuts at: 3106 3106 Size: 5770 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2527 2684 2743 2527 Size: 157 59 5554 Fragments arranged by size: 5554 157 59 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5770 XmaI C'CCGG_G Cuts at: 1870 2640 3095 1870 Size: 770 455 4545 Fragments arranged by size: 4545 770 455 XmnI GAAnn'nnTTC Cuts at: 2904 3899 2904 Size: 995 4775 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3578 276 Size: 53 83 186 936 2044 2468 Fragments arranged by size: 2468 2044 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SanDI SexAI SfiI SfoI SrfI SwaI