(Circular) (Six-base) MAPSORT of: pFN21AB8255.seq Check: 6736 from: 1 to: 5656 pFN21AB8255 5656 bp With 182 enzymes: SgfI * February 27, 2009 14:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2926 2926 Size: 5656 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3466 278 Size: 53 83 186 936 1930 2468 Fragments arranged by size: 2468 1930 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2999 1937 Size: 1062 4594 Acc65I G'GTAC_C Cuts at: 2977 2977 Size: 5656 AclI AA'CG_TT Cuts at: 1563 3783 4156 1563 Size: 2220 373 3063 Fragments arranged by size: 3063 2220 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2576 2710 2834 3719 5103 5371 1632 Size: 944 134 124 885 1384 268 1917 Fragments arranged by size: 1917 1384 944 885 268 134 124 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5459 AflIII A'CryG_T Cuts at: 1203 2953 4555 5532 1203 Size: 1750 1602 977 1327 Fragments arranged by size: 1750 1602 1327 977 AgeI A'CCGG_T Cuts at: 4487 4487 Size: 5656 AhdI GACnn_n'nnGTC Cuts at: 4385 4385 Size: 5656 AleI CACnn'nnGTG Cuts at: 1166 2857 1166 Size: 1691 3965 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5656 AlwNI CAG_nnn'CTG Cuts at: 2896 3399 4971 2896 Size: 503 1572 3581 Fragments arranged by size: 3581 1572 503 ApaLI G'TGCA_C Cuts at: 2860 3713 4869 2860 Size: 853 1156 3647 Fragments arranged by size: 3647 1156 853 ApoI r'AATT_y Cuts at: 1493 2163 2965 3191 3343 5452 1493 Size: 670 802 226 152 2109 1697 Fragments arranged by size: 2109 1697 802 670 226 152 AseI AT'TA_AT Cuts at: 160 1032 4210 160 Size: 872 3178 1606 Fragments arranged by size: 3178 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5656 AvaI C'yCGr_G Cuts at: 1870 1943 2109 2376 2981 1870 Size: 73 166 267 605 4545 Fragments arranged by size: 4545 605 267 166 73 BamHI G'GATC_C Cuts at: 2615 2986 3353 2615 Size: 371 367 4918 Fragments arranged by size: 4918 371 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2030 2288 2977 4432 618 Size: 325 217 627 243 258 689 1455 1842 Fragments arranged by size: 1842 1455 689 627 325 258 243 217 BanII G_rGCy'C Cuts at: 1391 2975 1391 Size: 1584 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2564 928 Size: 971 665 4020 Fragments arranged by size: 4020 971 665 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3847 3881 1903 Size: 34 1910 34 3678 Fragments arranged by size: 3678 1910 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3548 4758 3548 Size: 1210 4446 BclI T'GATC_A Cuts at: 1548 1548 Size: 5656 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2049 2580 4267 Size: 107 122 71 631 605 377 531 1687 Cuts at: 4267 136 Size: 1525 Fragments arranged by size: 1687 1525 631 605 531 377 122 107 71 BglII A'GATC_T Cuts at: 2775 5651 2775 Size: 2876 2780 BlpI GC'TnA_GC Cuts at: 3073 3073 Size: 5656 Bme1580I G_kGCm'C Cuts at: 1790 2291 2864 3717 4873 1790 Size: 501 573 853 1156 2573 Fragments arranged by size: 2573 1156 853 573 501 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5656 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2851 3387 4345 Size: 416 53 83 399 24 1434 536 958 Cuts at: 4345 442 Size: 1753 Fragments arranged by size: 1753 1434 958 536 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5656 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2461 2461 Size: 5656 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2592 2652 4316 1239 Size: 1353 60 1664 2579 Fragments arranged by size: 2579 1664 1353 60 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2021 3781 4646 4944 2021 Size: 1760 865 298 2733 Fragments arranged by size: 2733 1760 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2144 4319 882 Size: 617 645 2175 2219 Fragments arranged by size: 2219 2175 645 617 BsaAI yAC'GTr Cuts at: 493 1204 3447 493 Size: 711 2243 2702 Fragments arranged by size: 2702 2243 711 BsaBI GATnn'nnATC Cuts at: 5650 5650 Size: 5656 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3463 3845 4480 275 Size: 53 83 186 936 1930 382 635 1451 Fragments arranged by size: 1930 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4089 4487 4761 4908 5319 4089 Size: 398 274 147 411 4426 Fragments arranged by size: 4426 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2590 2620 1178 Size: 30 165 30 1187 30 4214 Fragments arranged by size: 4214 1187 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2209 2335 1186 Size: 1023 126 4507 Fragments arranged by size: 4507 1023 126 BseYI C'CCAG_C Cuts at: 2052 2511 2765 4859 5355 2052 Size: 459 254 2094 496 2353 Fragments arranged by size: 2353 2094 496 459 254 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5083 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3106 3867 4895 1540 Size: 275 186 1105 761 1028 2301 Fragments arranged by size: 2301 1105 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2179 2864 3717 3802 4873 2179 Size: 685 853 85 1071 2962 Fragments arranged by size: 2962 1071 853 685 85 BsmI GAATG_Cn' Cuts at: 3167 3260 3167 Size: 93 5563 BsmBI CGTCTCn'nnnn_ Cuts at: 2506 2506 Size: 5656 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2179 2291 2864 2975 3717 3802 4873 Size: 399 389 112 573 111 742 85 1071 Cuts at: 4873 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 573 399 389 112 111 85 BspEI T'CCGG_A Cuts at: 5319 5319 Size: 5656 BspHI T'CATG_A Cuts at: 3545 5275 3545 Size: 1730 3926 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2926 3011 844 Size: 2082 85 3489 Fragments arranged by size: 3489 2082 85 BsrBI CCG'CTC Cuts at: 1394 1823 3543 1394 Size: 429 1720 3507 Fragments arranged by size: 3507 1720 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4151 4325 65 Size: 1183 2903 174 1396 Fragments arranged by size: 2903 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2355 4300 4487 1700 Size: 110 545 1945 187 2869 Fragments arranged by size: 2869 1945 545 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5656 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5656 BssSI C'ACGA_G Cuts at: 2296 3716 4728 2296 Size: 1420 1012 3224 Fragments arranged by size: 3224 1420 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5656 BstBI TT'CG_AA Cuts at: 4460 4460 Size: 5656 BstEII G'GTnAC_C Cuts at: 1184 2056 2938 1184 Size: 872 882 3902 Fragments arranged by size: 3902 882 872 BstYI r'GATC_y Cuts at: 1972 2275 2372 2615 2662 2775 2986 3353 3738 Size: 303 97 243 47 113 211 367 385 Cuts at: 3738 3755 5207 5293 5305 5651 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 303 243 211 113 97 86 47 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5104 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4917 Fragments arranged by size: 4917 683 56 BtsI GCAGTG_nn' Cuts at: 3172 3966 3986 3172 Size: 794 20 4842 Fragments arranged by size: 4842 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5656 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5621 DraI TTT'AAA Cuts at: 2960 2960 Size: 5656 DraIII CAC_nnn'GTG Cuts at: 2174 2174 Size: 5656 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2522 4663 809 Size: 507 1206 2141 1802 Fragments arranged by size: 2141 1802 1206 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2227 2572 2644 Size: 54 1425 50 275 18 397 345 72 Cuts at: 2644 2887 3103 3992 5621 8 Size: 243 216 889 1629 43 Fragments arranged by size: 1629 1425 889 397 345 275 243 216 72 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3103 1537 Size: 275 1291 4090 Fragments arranged by size: 4090 1291 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3586 1353 Size: 2233 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2092 4243 4617 4763 387 Size: 1064 138 503 2151 374 146 1280 Fragments arranged by size: 2151 1280 1064 503 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2576 2592 2652 2710 2834 3719 4316 Size: 393 944 16 60 58 124 885 597 Cuts at: 4316 5103 5371 1239 Size: 787 268 1524 Fragments arranged by size: 1524 944 885 787 597 393 268 124 60 58 16 EcoO109I rG'GnC_Cy Cuts at: 2762 2762 Size: 5656 EcoRI G'AATT_C Cuts at: 2163 2965 3343 2163 Size: 802 378 4476 Fragments arranged by size: 4476 802 378 FspI TGC'GCA Cuts at: 4162 4162 Size: 5656 HaeII r_GCGC'y Cuts at: 1678 4803 1678 Size: 3125 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2441 2473 2590 2622 4297 Size: 32 135 32 869 32 117 32 1675 Cuts at: 4297 4329 4371 4403 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1675 869 135 117 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3000 3246 669 Size: 988 281 1062 246 3079 Fragments arranged by size: 3079 1062 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5656 HpaI GTT'AAC Cuts at: 3246 3246 Size: 5656 KpnI G_GTAC'C Cuts at: 2981 2981 Size: 5656 MfeI C'AATT_G Cuts at: 3255 3255 Size: 5656 MluI A'CGCG_T Cuts at: 2953 5532 2953 Size: 2579 3077 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4770 4954 1305 Size: 3465 184 2007 Fragments arranged by size: 3465 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2229 2574 2646 2889 10 Size: 54 1425 343 397 345 72 243 2777 Fragments arranged by size: 2777 1425 397 345 343 243 72 54 MslI CAynn'nnrTG Cuts at: 518 1166 2126 2857 3614 3973 4132 518 Size: 648 960 731 757 359 159 2042 Fragments arranged by size: 2042 960 757 731 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3072 3749 4897 5142 1606 Size: 1466 677 1148 245 2120 Fragments arranged by size: 2120 1466 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5104 NdeI CA'TA_TG Cuts at: 387 387 Size: 5656 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5656 NotI GC'GGCC_GC Cuts at: 3103 3103 Size: 5656 NspI r_CATG'y Cuts at: 1132 1229 3014 4559 1132 Size: 97 1785 1545 2229 Fragments arranged by size: 2229 1785 1545 97 PasI CC'CwG_GG Cuts at: 1348 2407 1348 Size: 1059 4597 PciI A'CATG_T Cuts at: 4555 4555 Size: 5656 PflMI CCAn_nnn'nTGG Cuts at: 1108 2658 1108 Size: 1550 4106 PfoI T'CCnGG_A Cuts at: 2085 2430 2085 Size: 345 5311 PmeI GTTT'AAAC Cuts at: 2960 2960 Size: 5656 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3725 3757 5238 5270 3725 Size: 32 1481 32 4111 Fragments arranged by size: 4111 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5656 PsiI TTA'TAA Cuts at: 3226 3226 Size: 5656 PstI C_TGCA'G Cuts at: 830 3008 830 Size: 2178 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5656 SalI G'TCGA_C Cuts at: 1936 2998 1936 Size: 1062 4594 SbfI CC_TGCA'GG Cuts at: 3008 3008 Size: 5656 ScaI AGT'ACT Cuts at: 3904 5616 3904 Size: 1712 3944 SexAI A'CCwGG_T Cuts at: 2919 2919 Size: 5656 SfcI C'TryA_G Cuts at: 826 1045 3004 4139 4820 5011 826 Size: 219 1959 1135 681 191 1471 Fragments arranged by size: 1959 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5656 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5656 SmaI CCC'GGG Cuts at: 1872 2983 1872 Size: 1111 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2036 2109 2376 3760 4661 4923 Size: 197 926 93 73 267 1384 901 262 Cuts at: 4923 820 Size: 1553 Fragments arranged by size: 1553 1384 926 901 267 262 197 93 73 SnaBI TAC'GTA Cuts at: 493 3447 493 Size: 2954 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5656 SphI G_CATG'C Cuts at: 1132 3014 1132 Size: 1882 3774 SspI AAT'ATT Cuts at: 5 52 3580 5 Size: 47 3528 2081 Fragments arranged by size: 3528 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5656 StyI C'CwwG_G Cuts at: 513 1065 2319 3095 513 Size: 552 1254 776 3074 Fragments arranged by size: 3074 1254 776 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2399 2443 3683 3700 3853 4038 4483 Size: 1255 819 44 1240 17 153 185 445 Cuts at: 4483 325 Size: 1498 Fragments arranged by size: 1498 1255 1240 819 445 185 153 44 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3902 5614 96 Size: 274 80 33 51 1443 1925 1712 138 Fragments arranged by size: 1925 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5616 5648 5616 Size: 32 5624 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5656 XbaI T'CTAG_A Cuts at: 2992 2992 Size: 5656 XhoI C'TCGA_G Cuts at: 1943 2109 2376 1943 Size: 166 267 5223 Fragments arranged by size: 5223 267 166 XmaI C'CCGG_G Cuts at: 1870 2981 1870 Size: 1111 4545 XmnI GAAnn'nnTTC Cuts at: 3785 3785 Size: 5656 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3464 276 Size: 53 83 186 936 1930 2468 Fragments arranged by size: 2468 1930 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI