Restriction map for
pFN21AB8249
(Circular) (Six-base) MAPSORT of: pFN21AB8249.seq Check: 6807 from: 1 to: 5422
pFN21AB8249 5422 bp
With 182 enzymes: SgfI *
February 27, 2009 14:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2395 2572 2395
Size: 177 5245
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3232 278
Size: 53 83 186 936 1696 2468
Fragments arranged by size:
2468 1696 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2765 1937
Size: 828 4594
Acc65I G'GTAC_C
Cuts at: 2743 2743
Size: 5422
AclI AA'CG_TT
Cuts at: 1563 3549 3922 1563
Size: 1986 373 3063
Fragments arranged by size:
3063 1986 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2035 2036 3485 4869 5137 1632
Size: 403 1 1449 1384 268 1917
Fragments arranged by size:
1917 1449 1384 403 268 1
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5225
AflIII A'CryG_T
Cuts at: 1203 4321 5298 1203
Size: 3118 977 1327
Fragments arranged by size:
3118 1327 977
AgeI A'CCGG_T
Cuts at: 4253 4253
Size: 5422
AhdI GACnn_n'nnGTC
Cuts at: 4151 4151
Size: 5422
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5422
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5422
AlwNI CAG_nnn'CTG
Cuts at: 2513 3165 4737 2513
Size: 652 1572 3198
Fragments arranged by size:
3198 1572 652
ApaLI G'TGCA_C
Cuts at: 2594 3479 4635 2594
Size: 885 1156 3381
Fragments arranged by size:
3381 1156 885
ApoI r'AATT_y
Cuts at: 1493 2377 2731 2957 3109 5218 1493
Size: 884 354 226 152 2109 1697
Fragments arranged by size:
2109 1697 884 354 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3976 160
Size: 872 2944 1606
Fragments arranged by size:
2944 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5422
AvaI C'yCGr_G
Cuts at: 1870 1943 2747 1870
Size: 73 804 4545
Fragments arranged by size:
4545 804 73
BamHI G'GATC_C
Cuts at: 2752 3119 2752
Size: 367 5055
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2743 4198 618
Size: 325 217 627 956 1455 1842
Fragments arranged by size:
1842 1455 956 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2652 2741 1391
Size: 1261 89 4072
Fragments arranged by size:
4072 1261 89
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4451
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3613 3647 1903
Size: 34 1676 34 3678
Fragments arranged by size:
3678 1676 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3314 4524 3314
Size: 1210 4212
BclI T'GATC_A
Cuts at: 1548 2409 1548
Size: 861 4561
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2009 4033 136
Size: 107 122 71 631 605 337 2024 1525
Fragments arranged by size:
2024 1525 631 605 337 122 107 71
BglII A'GATC_T
Cuts at: 5417 5417
Size: 5422
BlpI GC'TnA_GC
Cuts at: 2839 2839
Size: 5422
Bme1580I G_kGCm'C
Cuts at: 1790 2598 3483 4639 1790
Size: 808 885 1156 2573
Fragments arranged by size:
2573 1156 885 808
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5422
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3153 4111 442
Size: 416 53 83 399 24 1736 958 1753
Fragments arranged by size:
1753 1736 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5422
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4082 1239
Size: 2843 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3547 4412 4710 3547
Size: 865 298 4259
Fragments arranged by size:
4259 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4085 882
Size: 617 2586 2219
Fragments arranged by size:
2586 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3213 493
Size: 711 2009 2702
Fragments arranged by size:
2702 2009 711
BsaBI GATnn'nnATC
Cuts at: 5416 5416
Size: 5422
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3229 3611 4246 275
Size: 53 83 186 936 1696 382 635 1451
Fragments arranged by size:
1696 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3855 4253 4527 4674 5085 3855
Size: 398 274 147 411 4192
Fragments arranged by size:
4192 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2588 2618 1178
Size: 30 165 30 1185 30 3982
Fragments arranged by size:
3982 1185 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2242 2399 2693 1186
Size: 1056 157 294 3915
Fragments arranged by size:
3915 1056 294 157
BseYI C'CCAG_C
Cuts at: 4625 5121 4625
Size: 496 4926
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4849
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2872 3633 4661 1540
Size: 275 186 871 761 1028 2301
Fragments arranged by size:
2301 1028 871 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2598 2652 3483 3568 4639 2598
Size: 54 831 85 1071 3381
Fragments arranged by size:
3381 1071 831 85 54
BsmI GAATG_Cn'
Cuts at: 2933 3026 2933
Size: 93 5329
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2598 2652 2741 3483 3568 4639 1391
Size: 399 808 54 89 742 85 1071 2174
Fragments arranged by size:
2174 1071 808 742 399 89 85 54
BspEI T'CCGG_A
Cuts at: 5085 5085
Size: 5422
BspHI T'CATG_A
Cuts at: 3311 5041 3311
Size: 1730 3692
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2395 2572 2718 2777 844
Size: 1551 177 146 59 3489
Fragments arranged by size:
3489 1551 177 146 59
BsrBI CCG'CTC
Cuts at: 1394 1823 2683 3309 1394
Size: 429 860 626 3507
Fragments arranged by size:
3507 860 626 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3917 4091 65
Size: 1183 2669 174 1396
Fragments arranged by size:
2669 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4066 4253 1700
Size: 110 2256 187 2869
Fragments arranged by size:
2869 2256 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5422
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5422
BssSI C'ACGA_G
Cuts at: 3482 4494 3482
Size: 1012 4410
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2513 794
Size: 1719 3703
BstBI TT'CG_AA
Cuts at: 4226 4226
Size: 5422
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5422
BstYI r'GATC_y
Cuts at: 1972 2752 3119 3504 3521 4973 5059 5071 5417
Size: 780 367 385 17 1452 86 12 346
Cuts at: 5417 1972
Size: 1977
Fragments arranged by size:
1977 1452 780 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2126 513
Size: 552 939 122 3809
Fragments arranged by size:
3809 939 552 122
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4683
Fragments arranged by size:
4683 683 56
BtsI GCAGTG_nn'
Cuts at: 2241 2938 3732 3752 2241
Size: 697 794 20 3911
Fragments arranged by size:
3911 794 697 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5422
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5387
DraI TTT'AAA
Cuts at: 2726 2726
Size: 5422
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4429 809
Size: 507 3113 1802
Fragments arranged by size:
3113 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2869 3758 5387
Size: 54 1425 50 275 18 1039 889 1629
Cuts at: 5387 8
Size: 43
Fragments arranged by size:
1629 1425 1039 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2869 1537
Size: 275 1057 4090
Fragments arranged by size:
4090 1057 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2012 2583 2640 3352 1353
Size: 659 571 57 712 3423
Fragments arranged by size:
3423 712 659 571 57
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4009 4383 4529 387
Size: 1064 138 2420 374 146 1280
Fragments arranged by size:
2420 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2650 2650
Size: 5422
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2035 2036 3485 4082 4869 5137 1239
Size: 393 403 1 1449 597 787 268 1524
Fragments arranged by size:
1524 1449 787 597 403 393 268 1
EcoNI CCTnn'n_nnAGG
Cuts at: 2714 2714
Size: 5422
EcoRI G'AATT_C
Cuts at: 2377 2731 3109 2377
Size: 354 378 4690
Fragments arranged by size:
4690 378 354
FspI TGC'GCA
Cuts at: 3928 3928
Size: 5422
HaeII r_GCGC'y
Cuts at: 1678 4569 1678
Size: 2891 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2243 2275 2525 2557 4063
Size: 32 135 32 671 32 250 32 1506
Cuts at: 4063 4095 4137 4169 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1506 671 250 135 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2766 3012 669
Size: 988 281 828 246 3079
Fragments arranged by size:
3079 988 828 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5422
HpaI GTT'AAC
Cuts at: 3012 3012
Size: 5422
KpnI G_GTAC'C
Cuts at: 2747 2747
Size: 5422
MfeI C'AATT_G
Cuts at: 3021 3021
Size: 5422
MluI A'CGCG_T
Cuts at: 5298 5298
Size: 5422
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2673 4536 4720 1305
Size: 1368 1863 184 2007
Fragments arranged by size:
2007 1863 1368 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3600
Fragments arranged by size:
3600 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2474 3380 3739 3898 518
Size: 648 1308 906 359 159 2042
Fragments arranged by size:
2042 1308 906 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2569 2838 3515 4663 4908 1606
Size: 963 269 677 1148 245 2120
Fragments arranged by size:
2120 1148 963 677 269 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2126 513
Size: 552 939 122 3809
Fragments arranged by size:
3809 939 552 122
NdeI CA'TA_TG
Cuts at: 387 2284 387
Size: 1897 3525
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5422
NotI GC'GGCC_GC
Cuts at: 2869 2869
Size: 5422
NspI r_CATG'y
Cuts at: 1132 1229 2780 4325 1132
Size: 97 1551 1545 2229
Fragments arranged by size:
2229 1551 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5422
PciI A'CATG_T
Cuts at: 4321 4321
Size: 5422
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5422
PfoI T'CCnGG_A
Cuts at: 2606 2606
Size: 5422
PmeI GTTT'AAAC
Cuts at: 2726 2726
Size: 5422
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3491 3523 5004 5036 3491
Size: 32 1481 32 3877
Fragments arranged by size:
3877 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5422
PsiI TTA'TAA
Cuts at: 2992 2992
Size: 5422
PstI C_TGCA'G
Cuts at: 830 2485 2583 2774 830
Size: 1655 98 191 3478
Fragments arranged by size:
3478 1655 191 98
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5422
PvuII CAG'CTG
Cuts at: 2569 2569
Size: 5422
SacI G_AGCT'C
Cuts at: 2652 2652
Size: 5422
SalI G'TCGA_C
Cuts at: 1936 2764 1936
Size: 828 4594
SapI GCTCTTCn'nnn_
Cuts at: 2640 2640
Size: 5422
SbfI CC_TGCA'GG
Cuts at: 2774 2774
Size: 5422
ScaI AGT'ACT
Cuts at: 2453 3670 5382 2453
Size: 1217 1712 2493
Fragments arranged by size:
2493 1712 1217
SfcI C'TryA_G
Cuts at: 826 1045 2481 2579 2770 3905 4586 4777 826
Size: 219 1436 98 191 1135 681 191 1471
Fragments arranged by size:
1471 1436 1135 681 219 191 191 98
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5422
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5422
SmaI CCC'GGG
Cuts at: 1872 2749 1872
Size: 877 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3526 4427 4689 820
Size: 197 926 1583 901 262 1553
Fragments arranged by size:
1583 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3213 493
Size: 2720 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5422
SphI G_CATG'C
Cuts at: 1132 2780 1132
Size: 1648 3774
SspI AAT'ATT
Cuts at: 5 52 3346 5
Size: 47 3294 2081
Fragments arranged by size:
3294 2081 47
StuI AGG'CCT
Cuts at: 1517 2540 2705 1517
Size: 1023 165 4234
Fragments arranged by size:
4234 1023 165
StyI C'CwwG_G
Cuts at: 513 1065 2004 2126 2861 513
Size: 552 939 122 735 3074
Fragments arranged by size:
3074 939 735 552 122
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3449 3466 3619 3804 4249 325
Size: 1255 1869 17 153 185 445 1498
Fragments arranged by size:
1869 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2451 3668 5380
Size: 274 80 33 51 1443 474 1217 1712
Cuts at: 5380 96
Size: 138
Fragments arranged by size:
1712 1443 1217 474 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2588 2620 5382 5414 2588
Size: 32 2762 32 2596
Fragments arranged by size:
2762 2596 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5422
XbaI T'CTAG_A
Cuts at: 2758 2758
Size: 5422
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5422
XmaI C'CCGG_G
Cuts at: 1870 2747 1870
Size: 877 4545
XmnI GAAnn'nnTTC
Cuts at: 3551 3551
Size: 5422
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3230 276
Size: 53 83 186 936 1696 2468
Fragments arranged by size:
2468 1696 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoRI FspI HaeII Hin4I HincII HindIII
HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I
NcoI NdeI NheI NotI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoO109I EcoRV FalI FseI FspAI KasI NaeI
NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI
SrfI SwaI XcmI