Restriction map for
pFN21AB8248
(Circular) (Six-base) MAPSORT of: pFN21AB8248.seq Check: 7490 from: 1 to: 5410
pFN21AB8248 5410 bp
With 182 enzymes: SgfI *
February 27, 2009 14:41 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2426 2426
Size: 5410
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3220 278
Size: 53 83 186 936 1684 2468
Fragments arranged by size:
2468 1684 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2753 1937
Size: 816 4594
Acc65I G'GTAC_C
Cuts at: 2731 2731
Size: 5410
AclI AA'CG_TT
Cuts at: 1563 3537 3910 1563
Size: 1974 373 3063
Fragments arranged by size:
3063 1974 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3473 4857 5125 1632
Size: 1841 1384 268 1917
Fragments arranged by size:
1917 1841 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5213
AflIII A'CryG_T
Cuts at: 1203 2304 2659 4309 5286 1203
Size: 1101 355 1650 977 1327
Fragments arranged by size:
1650 1327 1101 977 355
AgeI A'CCGG_T
Cuts at: 4241 4241
Size: 5410
AhdI GACnn_n'nnGTC
Cuts at: 4139 4139
Size: 5410
AleI CACnn'nnGTG
Cuts at: 1166 2357 1166
Size: 1191 4219
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5410
AlwNI CAG_nnn'CTG
Cuts at: 2113 2615 2632 3153 4725 2113
Size: 502 17 521 1572 2798
Fragments arranged by size:
2798 1572 521 502 17
ApaLI G'TGCA_C
Cuts at: 3467 4623 3467
Size: 1156 4254
ApoI r'AATT_y
Cuts at: 1493 2719 2945 3097 5206 1493
Size: 1226 226 152 2109 1697
Fragments arranged by size:
2109 1697 1226 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3964 160
Size: 872 2932 1606
Fragments arranged by size:
2932 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5410
AvaI C'yCGr_G
Cuts at: 1870 1943 2067 2535 2735 1870
Size: 73 124 468 200 4545
Fragments arranged by size:
4545 468 200 124 73
BamHI G'GATC_C
Cuts at: 2506 2740 3107 2506
Size: 234 367 4809
Fragments arranged by size:
4809 367 234
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2731 4186 618
Size: 325 217 627 944 1455 1842
Fragments arranged by size:
1842 1455 944 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2455 2604 2622 2729 1391
Size: 1064 149 18 107 4072
Fragments arranged by size:
4072 1064 149 107 18
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2656 928
Size: 971 757 3682
Fragments arranged by size:
3682 971 757
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3601 3635 1903
Size: 34 1664 34 3678
Fragments arranged by size:
3678 1664 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3302 4512 3302
Size: 1210 4200
BclI T'GATC_A
Cuts at: 1548 2295 1548
Size: 747 4663
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2420 4021 136
Size: 107 122 71 631 605 748 1601 1525
Fragments arranged by size:
1601 1525 748 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5405 5405
Size: 5410
BlpI GC'TnA_GC
Cuts at: 2827 2827
Size: 5410
Bme1580I G_kGCm'C
Cuts at: 1790 2330 2445 3471 4627 1790
Size: 540 115 1026 1156 2573
Fragments arranged by size:
2573 1156 1026 540 115
BmgBI CAC'GTC
Cuts at: 1318 2662 1318
Size: 1344 4066
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3141 4099 442
Size: 416 53 83 399 24 1724 958 1753
Fragments arranged by size:
1753 1724 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5410
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2606 4070 1239
Size: 1367 1464 2579
Fragments arranged by size:
2579 1464 1367
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3535 4400 4698 3535
Size: 865 298 4247
Fragments arranged by size:
4247 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4073 882
Size: 617 2574 2219
Fragments arranged by size:
2574 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2307 3201 493
Size: 711 1103 894 2702
Fragments arranged by size:
2702 1103 894 711
BsaBI GATnn'nnATC
Cuts at: 5404 5404
Size: 5410
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2028 3217 3599 4234
Size: 53 83 186 936 495 1189 382 635
Cuts at: 4234 275
Size: 1451
Fragments arranged by size:
1451 1189 936 635 495 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3843 4241 4515 4662 5073 3843
Size: 398 274 147 411 4180
Fragments arranged by size:
4180 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5185
Fragments arranged by size:
5185 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2024 2027 1186
Size: 838 3 4569
Fragments arranged by size:
4569 838 3
BseYI C'CCAG_C
Cuts at: 2323 4613 5109 2323
Size: 2290 496 2624
Fragments arranged by size:
2624 2290 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4837
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2190 2860 3621 4649 1540
Size: 275 186 189 670 761 1028 2301
Fragments arranged by size:
2301 1028 761 670 275 189 186
BsiHKAI G_wGCw'C
Cuts at: 2622 3471 3556 4627 2622
Size: 849 85 1071 3405
Fragments arranged by size:
3405 1071 849 85
BsmI GAATG_Cn'
Cuts at: 2413 2921 3014 2413
Size: 508 93 4809
Fragments arranged by size:
4809 508 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2009 2009
Size: 5410
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2330 2445 2455 2604 2622 2729 3471
Size: 399 540 115 10 149 18 107 742
Cuts at: 3471 3556 4627 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 540 399 149 115 107
85 18 10
BspEI T'CCGG_A
Cuts at: 5073 5073
Size: 5410
BspHI T'CATG_A
Cuts at: 3299 5029 3299
Size: 1730 3680
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2121 2356 2426 2765 844
Size: 1277 235 70 339 3489
Fragments arranged by size:
3489 1277 339 235 70
BsrBI CCG'CTC
Cuts at: 1394 1823 2290 3297 1394
Size: 429 467 1007 3507
Fragments arranged by size:
3507 1007 467 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3905 4079 65
Size: 1183 2657 174 1396
Fragments arranged by size:
2657 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2185 2381 4054 4241 1700
Size: 110 375 196 1673 187 2869
Fragments arranged by size:
2869 1673 375 196 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5410
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5410
BssSI C'ACGA_G
Cuts at: 2498 3470 4482 2498
Size: 972 1012 3426
Fragments arranged by size:
3426 1012 972
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5410
BstBI TT'CG_AA
Cuts at: 4214 4214
Size: 5410
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5410
BstXI CCAn_nnnn'nTGG
Cuts at: 2356 2356
Size: 5410
BstYI r'GATC_y
Cuts at: 1972 2506 2740 3107 3492 3509 4961 5047 5059
Size: 534 234 367 385 17 1452 86 12
Cuts at: 5059 5405 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 534 385 367 346 234 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2243 2243
Size: 5410
BtgI C'CryG_G
Cuts at: 513 1065 2004 2355 513
Size: 552 939 351 3568
Fragments arranged by size:
3568 939 552 351
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2391 487
Size: 683 56 1165 3506
Fragments arranged by size:
3506 1165 683 56
BtsI GCAGTG_nn'
Cuts at: 2633 2926 3720 3740 2633
Size: 293 794 20 4303
Fragments arranged by size:
4303 794 293 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5410
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5375
DraI TTT'AAA
Cuts at: 2714 2714
Size: 5410
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 2162 4417 809
Size: 507 846 2255 1802
Fragments arranged by size:
2255 1802 846 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2123 2187 2857
Size: 54 1425 50 275 18 293 64 670
Cuts at: 2857 3746 5375 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 670 293 275 64 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2187 2857 1537
Size: 275 375 670 4090
Fragments arranged by size:
4090 670 375 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2273 2462 3340 1353
Size: 920 189 878 3423
Fragments arranged by size:
3423 920 878 189
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3997 4371 4517 387
Size: 1064 138 2408 374 146 1280
Fragments arranged by size:
2408 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2620 2620
Size: 5410
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2606 3473 4070 4857 5125 1239
Size: 393 974 867 597 787 268 1524
Fragments arranged by size:
1524 974 867 787 597 393 268
EcoO109I rG'GnC_Cy
Cuts at: 2072 2493 2072
Size: 421 4989
EcoRI G'AATT_C
Cuts at: 2719 3097 2719
Size: 378 5032
FseI GG_CCGG'CC
Cuts at: 2189 2189
Size: 5410
FspI TGC'GCA
Cuts at: 3916 3916
Size: 5410
HaeII r_GCGC'y
Cuts at: 1678 4557 1678
Size: 2879 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2369 2401 2498 2530 4051
Size: 32 135 32 797 32 97 32 1521
Cuts at: 4051 4083 4125 4157 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1521 797 135 97 42 32 32
32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2754 3000 669
Size: 988 281 816 246 3079
Fragments arranged by size:
3079 988 816 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5410
HpaI GTT'AAC
Cuts at: 3000 3000
Size: 5410
KpnI G_GTAC'C
Cuts at: 2735 2735
Size: 5410
MfeI C'AATT_G
Cuts at: 3009 3009
Size: 5410
MluI A'CGCG_T
Cuts at: 5286 5286
Size: 5410
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4524 4708 1305
Size: 3219 184 2007
Fragments arranged by size:
3219 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3588
Fragments arranged by size:
3588 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2354 2357 3368 3727 3886 518
Size: 648 1188 3 1011 359 159 2042
Fragments arranged by size:
2042 1188 1011 648 359 159 3
MspA1I CmG'CkG
Cuts at: 1606 2826 3503 4651 4896 1606
Size: 1220 677 1148 245 2120
Fragments arranged by size:
2120 1220 1148 677 245
NaeI GCC'GGC
Cuts at: 2187 2383 2187
Size: 196 5214
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3919
Fragments arranged by size:
3919 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5410
NgoMIV G'CCGG_C
Cuts at: 2185 2381 2185
Size: 196 5214
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5410
NotI GC'GGCC_GC
Cuts at: 2857 2857
Size: 5410
NspI r_CATG'y
Cuts at: 1132 1229 2768 4313 1132
Size: 97 1539 1545 2229
Fragments arranged by size:
2229 1545 1539 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5410
PciI A'CATG_T
Cuts at: 4309 4309
Size: 5410
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 5410
PmeI GTTT'AAAC
Cuts at: 2714 2714
Size: 5410
PmlI CAC'GTG
Cuts at: 2307 2307
Size: 5410
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3479 3511 4992 5024 3479
Size: 32 1481 32 3865
Fragments arranged by size:
3865 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2072 2493 2072
Size: 421 4989
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5410
PsiI TTA'TAA
Cuts at: 2980 2980
Size: 5410
PstI C_TGCA'G
Cuts at: 830 2084 2367 2691 2762 830
Size: 1254 283 324 71 3478
Fragments arranged by size:
3478 1254 324 283 71
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5410
SacI G_AGCT'C
Cuts at: 2622 2622
Size: 5410
SalI G'TCGA_C
Cuts at: 1936 2752 1936
Size: 816 4594
SanDI GG'GwC_CC
Cuts at: 2493 2493
Size: 5410
SapI GCTCTTCn'nnn_
Cuts at: 2273 2273
Size: 5410
SbfI CC_TGCA'GG
Cuts at: 2762 2762
Size: 5410
ScaI AGT'ACT
Cuts at: 3658 5370 3658
Size: 1712 3698
SfcI C'TryA_G
Cuts at: 826 1045 2080 2363 2687 2758 3893 4574 4765
Size: 219 1035 283 324 71 1135 681 191
Cuts at: 4765 826
Size: 1471
Fragments arranged by size:
1471 1135 1035 681 324 283 219 191
71
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5410
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5410
SmaI CCC'GGG
Cuts at: 1872 2537 2737 1872
Size: 665 200 4545
Fragments arranged by size:
4545 665 200
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3514 4415 4677 820
Size: 197 926 1571 901 262 1553
Fragments arranged by size:
1571 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3201 493
Size: 2708 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5410
SphI G_CATG'C
Cuts at: 1132 2768 1132
Size: 1636 3774
SrfI GCCC'GGGC
Cuts at: 2537 2537
Size: 5410
SspI AAT'ATT
Cuts at: 5 52 3334 5
Size: 47 3282 2081
Fragments arranged by size:
3282 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5410
StyI C'CwwG_G
Cuts at: 513 1065 2004 2595 2849 513
Size: 552 939 591 254 3074
Fragments arranged by size:
3074 939 591 552 254
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3437 3454 3607 3792 4237 325
Size: 1255 1857 17 153 185 445 1498
Fragments arranged by size:
1857 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3656 5368 96
Size: 274 80 33 51 1443 1679 1712 138
Fragments arranged by size:
1712 1679 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5370 5402 5370
Size: 32 5378
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5410
XbaI T'CTAG_A
Cuts at: 2746 2746
Size: 5410
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5410
XmaI C'CCGG_G
Cuts at: 1870 2535 2735 1870
Size: 665 200 4545
Fragments arranged by size:
4545 665 200
XmnI GAAnn'nnTTC
Cuts at: 3539 3539
Size: 5410
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3218 276
Size: 53 83 186 936 1684 2468
Fragments arranged by size:
2468 1684 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FseI FspI
HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI
MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV
NheI NotI NspI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PstI PvuI SacI SalI
SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BstZ17I DraIII EcoNI EcoRV
FalI FspAI KasI NarI NruI NsiI PacI PfoI
PspOMI PspXI PsrI PvuII RsrII SacII SexAI SfiI
SfoI SwaI XcmI