(Circular) (Six-base) MAPSORT of: pFN21AB8246.seq Check: 9312 from: 1 to: 5440 pFN21AB8246 5440 bp With 182 enzymes: SgfI * February 27, 2009 14:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2339 2339 Size: 5440 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3250 278 Size: 53 83 186 936 1714 2468 Fragments arranged by size: 2468 1714 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2783 1937 Size: 846 4594 Acc65I G'GTAC_C Cuts at: 2218 2761 2218 Size: 543 4897 AclI AA'CG_TT Cuts at: 1563 3567 3940 1563 Size: 2004 373 3063 Fragments arranged by size: 3063 2004 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2401 2449 3503 4887 5155 1632 Size: 769 48 1054 1384 268 1917 Fragments arranged by size: 1917 1384 1054 769 268 48 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5243 AflIII A'CryG_T Cuts at: 1203 2154 4339 5316 1203 Size: 951 2185 977 1327 Fragments arranged by size: 2185 1327 977 951 AgeI A'CCGG_T Cuts at: 4271 4271 Size: 5440 AhdI GACnn_n'nnGTC Cuts at: 4169 4169 Size: 5440 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5440 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5440 AlwNI CAG_nnn'CTG Cuts at: 2245 3183 4755 2245 Size: 938 1572 2930 Fragments arranged by size: 2930 1572 938 ApaLI G'TGCA_C Cuts at: 2150 3497 4653 2150 Size: 1347 1156 2937 Fragments arranged by size: 2937 1347 1156 ApoI r'AATT_y Cuts at: 1493 2385 2749 2975 3127 5236 1493 Size: 892 364 226 152 2109 1697 Fragments arranged by size: 2109 1697 892 364 226 152 AseI AT'TA_AT Cuts at: 160 1032 3994 160 Size: 872 2962 1606 Fragments arranged by size: 2962 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5440 AvaI C'yCGr_G Cuts at: 1870 1943 2765 1870 Size: 73 822 4545 Fragments arranged by size: 4545 822 73 BamHI G'GATC_C Cuts at: 2770 3137 2770 Size: 367 5073 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2218 2761 4216 618 Size: 325 217 627 431 543 1455 1842 Fragments arranged by size: 1842 1455 627 543 431 325 217 BanII G_rGCy'C Cuts at: 1391 2130 2759 1391 Size: 739 629 4072 Fragments arranged by size: 4072 739 629 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2407 2455 2514 2659 928 Size: 971 508 48 59 145 3709 Fragments arranged by size: 3709 971 508 145 59 48 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2237 2271 3631 3665 1903 Size: 34 300 34 1360 34 3678 Fragments arranged by size: 3678 1360 300 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3332 4542 3332 Size: 1210 4230 BclI T'GATC_A Cuts at: 1548 1548 Size: 5440 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4051 136 Size: 107 122 71 631 605 2379 1525 Fragments arranged by size: 2379 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5435 5435 Size: 5440 BlpI GC'TnA_GC Cuts at: 2857 2857 Size: 5440 Bme1580I G_kGCm'C Cuts at: 1790 2154 3501 4657 1790 Size: 364 1347 1156 2573 Fragments arranged by size: 2573 1347 1156 364 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5440 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3171 4129 442 Size: 416 53 83 399 24 1754 958 1753 Fragments arranged by size: 1754 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5440 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2323 2542 2323 Size: 219 5221 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4100 1239 Size: 2861 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2387 3565 4430 4728 2387 Size: 1178 865 298 3099 Fragments arranged by size: 3099 1178 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2216 4103 882 Size: 617 717 1887 2219 Fragments arranged by size: 2219 1887 717 617 BsaAI yAC'GTr Cuts at: 493 1204 2344 3231 493 Size: 711 1140 887 2702 Fragments arranged by size: 2702 1140 887 711 BsaBI GATnn'nnATC Cuts at: 5434 5434 Size: 5440 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3247 3629 4264 275 Size: 53 83 186 936 1714 382 635 1451 Fragments arranged by size: 1714 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3873 4271 4545 4692 5103 3873 Size: 398 274 147 411 4210 Fragments arranged by size: 4210 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2469 2499 1178 Size: 30 165 30 1066 30 4119 Fragments arranged by size: 4119 1066 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2312 1186 Size: 1126 4314 BseYI C'CCAG_C Cuts at: 2588 2685 4643 5139 2588 Size: 97 1958 496 2889 Fragments arranged by size: 2889 1958 496 97 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2300 1163 Size: 573 564 4303 Fragments arranged by size: 4303 573 564 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2088 2890 3651 4679 1540 Size: 275 186 87 802 761 1028 2301 Fragments arranged by size: 2301 1028 802 761 275 186 87 BsiHKAI G_wGCw'C Cuts at: 2130 2154 2403 3501 3586 4657 2130 Size: 24 249 1098 85 1071 2913 Fragments arranged by size: 2913 1098 1071 249 85 24 BsmI GAATG_Cn' Cuts at: 2951 3044 2951 Size: 93 5347 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2130 2154 2403 2759 3501 3586 4657 Size: 399 340 24 249 356 742 85 1071 Cuts at: 4657 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 356 340 249 85 24 BspEI T'CCGG_A Cuts at: 5103 5103 Size: 5440 BspHI T'CATG_A Cuts at: 3329 5059 3329 Size: 1730 3710 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2339 2795 844 Size: 1495 456 3489 Fragments arranged by size: 3489 1495 456 BsrBI CCG'CTC Cuts at: 1394 1823 2302 3327 1394 Size: 429 479 1025 3507 Fragments arranged by size: 3507 1025 479 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2326 2341 2697 3935 4109 65 Size: 1183 1078 15 356 1238 174 1396 Fragments arranged by size: 1396 1238 1183 1078 356 174 15 BsrFI r'CCGG_y Cuts at: 1700 1810 2165 4084 4271 1700 Size: 110 355 1919 187 2869 Fragments arranged by size: 2869 1919 355 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5440 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5440 BssSI C'ACGA_G Cuts at: 3500 4512 3500 Size: 1012 4428 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5440 BstBI TT'CG_AA Cuts at: 4244 4244 Size: 5440 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5440 BstXI CCAn_nnnn'nTGG Cuts at: 2146 2146 Size: 5440 BstYI r'GATC_y Cuts at: 1972 2770 3137 3522 3539 4991 5077 5089 5435 Size: 798 367 385 17 1452 86 12 346 Cuts at: 5435 1972 Size: 1977 Fragments arranged by size: 1977 1452 798 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2145 513 Size: 552 939 141 3808 Fragments arranged by size: 3808 939 552 141 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4701 Fragments arranged by size: 4701 683 56 BtsI GCAGTG_nn' Cuts at: 2578 2956 3750 3770 2578 Size: 378 794 20 4248 Fragments arranged by size: 4248 794 378 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5440 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5405 DraI TTT'AAA Cuts at: 2744 2744 Size: 5440 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4447 809 Size: 507 3131 1802 Fragments arranged by size: 3131 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2430 2887 3776 Size: 54 1425 50 275 18 600 457 889 Cuts at: 3776 5405 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 600 457 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2887 1537 Size: 275 1075 4090 Fragments arranged by size: 4090 1075 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2535 3370 1353 Size: 1182 835 3423 Fragments arranged by size: 3423 1182 835 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4027 4401 4547 387 Size: 1064 138 2438 374 146 1280 Fragments arranged by size: 2438 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2128 2128 Size: 5440 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2401 2449 3503 4100 4887 5155 1239 Size: 393 769 48 1054 597 787 268 1524 Fragments arranged by size: 1524 1054 787 769 597 393 268 48 EcoRI G'AATT_C Cuts at: 2749 3127 2749 Size: 378 5062 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2561 2561 Size: 5440 FspI TGC'GCA Cuts at: 3946 3946 Size: 5440 HaeII r_GCGC'y Cuts at: 1678 4587 1678 Size: 2909 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2507 2539 4081 4113 4155 Size: 32 135 32 935 32 1542 32 42 Cuts at: 4155 4187 1373 Size: 32 2626 Fragments arranged by size: 2626 1542 935 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2784 3030 669 Size: 988 281 846 246 3079 Fragments arranged by size: 3079 988 846 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5440 HpaI GTT'AAC Cuts at: 3030 3030 Size: 5440 KpnI G_GTAC'C Cuts at: 2222 2765 2222 Size: 543 4897 MfeI C'AATT_G Cuts at: 2502 3039 2502 Size: 537 4903 MluI A'CGCG_T Cuts at: 2154 5316 2154 Size: 3162 2278 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4554 4738 1305 Size: 3249 184 2007 Fragments arranged by size: 3249 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3618 Fragments arranged by size: 3618 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2144 2192 2708 3398 3757 3916 518 Size: 648 978 48 516 690 359 159 2042 Fragments arranged by size: 2042 978 690 648 516 359 159 48 MspA1I CmG'CkG Cuts at: 1606 2856 3533 4681 4926 1606 Size: 1250 677 1148 245 2120 Fragments arranged by size: 2120 1250 1148 677 245 NaeI GCC'GGC Cuts at: 2167 2167 Size: 5440 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3949 Fragments arranged by size: 3949 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5440 NgoMIV G'CCGG_C Cuts at: 2165 2165 Size: 5440 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5440 NotI GC'GGCC_GC Cuts at: 2887 2887 Size: 5440 NspI r_CATG'y Cuts at: 1132 1229 2191 2713 2798 4343 1132 Size: 97 962 522 85 1545 2229 Fragments arranged by size: 2229 1545 962 522 97 85 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5440 PciI A'CATG_T Cuts at: 4339 4339 Size: 5440 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5440 PmeI GTTT'AAAC Cuts at: 2744 2744 Size: 5440 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3509 3541 5022 5054 3509 Size: 32 1481 32 3895 Fragments arranged by size: 3895 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5440 PsiI TTA'TAA Cuts at: 3010 3010 Size: 5440 PstI C_TGCA'G Cuts at: 830 2792 830 Size: 1962 3478 PvuI CG_AT'CG Cuts at: 2001 2088 2001 Size: 87 5353 RsrII CG'GwC_CG Cuts at: 2011 2011 Size: 5440 SacI G_AGCT'C Cuts at: 2130 2130 Size: 5440 SalI G'TCGA_C Cuts at: 1936 2782 1936 Size: 846 4594 SbfI CC_TGCA'GG Cuts at: 2792 2792 Size: 5440 ScaI AGT'ACT Cuts at: 3688 5400 3688 Size: 1712 3728 SexAI A'CCwGG_T Cuts at: 2436 2436 Size: 5440 SfcI C'TryA_G Cuts at: 826 1045 2107 2788 3923 4604 4795 826 Size: 219 1062 681 1135 681 191 1471 Fragments arranged by size: 1471 1135 1062 681 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5440 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5440 SmaI CCC'GGG Cuts at: 1872 2767 1872 Size: 895 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2402 3544 4445 4707 820 Size: 197 926 459 1142 901 262 1553 Fragments arranged by size: 1553 1142 926 901 459 262 197 SnaBI TAC'GTA Cuts at: 493 3231 493 Size: 2738 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5440 SphI G_CATG'C Cuts at: 1132 2713 2798 1132 Size: 1581 85 3774 Fragments arranged by size: 3774 1581 85 SspI AAT'ATT Cuts at: 5 52 3364 5 Size: 47 3312 2081 Fragments arranged by size: 3312 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5440 StyI C'CwwG_G Cuts at: 513 1065 2004 2313 2349 2879 513 Size: 552 939 309 36 530 3074 Fragments arranged by size: 3074 939 552 530 309 36 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2373 3467 3484 3637 3822 4267 325 Size: 1255 793 1094 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1094 793 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3686 5398 96 Size: 274 80 33 51 1443 1709 1712 138 Fragments arranged by size: 1712 1709 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2469 2501 5400 5432 2469 Size: 32 2899 32 2477 Fragments arranged by size: 2899 2477 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5440 XbaI T'CTAG_A Cuts at: 2776 2776 Size: 5440 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5440 XmaI C'CCGG_G Cuts at: 1870 2765 1870 Size: 895 4545 XmnI GAAnn'nnTTC Cuts at: 2385 3569 2385 Size: 1184 4256 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3248 276 Size: 53 83 186 936 1714 2468 Fragments arranged by size: 2468 1714 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI RsrII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoNI EcoO109I EcoRV FseI FspAI KasI NarI NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI PvuII SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI