Restriction map for
pFN21AB8245
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# NO CUT ENZYMES ( 191 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AfaI AflII
AflIII AgeI Alw44I AlwI AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspHI AspI
AvaI AvaII AvrII BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiHKAI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DdeI DraI DraII
DraIII DrdI DsaI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRV EcoT14I EcoT22I FauI
FbaI FseI HaeII HapII HgaI
Hin1I HincII HindII HindIII HinfI
HpaI HpaII KasI KpnI KspI
MamI MfeI MflI MluI MroI
MspA1I MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NlaIV NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuII RcaI RsaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StyI SwaI
TaqI TfiI Tth111I TthHB8I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AcsI 296 GAATTC [GA]^AATT[CT]
AhdI 511 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 296 GAATTC [GA]^AATT[CT]
Asp700I 292 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 511 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 53 TGCGCA TGC^GCA
BalI 401 TGGCCA TGG^CCA
BfaI 427 CTAG C^TAG
BseRI 15 CTCCTC GAGGAG(10/8)
BsiEI 285 CGATCG CG[GA][CT]^CG
BsiYI 139 CCTACCATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 139 CCTACCATTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 438 GTCTC GTCTC(1/5)
BssKI 255 CCAGG ^CC[ACGT]GG
BstAPI 321 GCAGAAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 255 CCAGG CC^[AT]GG
Cac8I 82 GCTGGC GC[ACGT]^[ACGT]GC
CfoI 54 GCGC GCG^C
Cfr13I 3 GGGCC G^G[ACGT]CC
DpnII 286 GATC ^GATC
EaeI 401 TGGCCA [CT]^GGCC[GA]
Eam1105I 511 GACTGGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 296 GAATTC G^AATTC
EcoRII 255 CCAGG ^CC[AT]GG
FspI 53 TGCGCA TGC^GCA
HhaI 54 GCGC GCG^C
HinP1I 54 GCGC G^CGC
HphI 128 TCACC GGTGA(8/7)
MaeI 427 CTAG C^TAG
MaeII 537 ACGT A^CGT
MboI 286 GATC ^GATC
MluNI 401 TGGCCA TGG^CCA
MscI 401 TGGCCA TGG^CCA
MseI 152 TTAA T^TAA
MslI 57 CATTTTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvaI 255 CCAGG CC^[AT]GG
NdeII 286 GATC ^GATC
NlaIII 455 CATG CATG^
PstI 319 CTGCAG CTGCA^G
PvuI 285 CGATCG CGAT^CG
Sau3AI 286 GATC ^GATC
Sau96I 3 GGGCC G^G[ACGT]CC
ScrFI 255 CCAGG CC^[ACGT]GG
SfcI 319 CTGCAG C^T[GA][CT]AG
StuI 223 AGGCCT AGG^CCT
Tru9I 152 TTAA T^TAA
Tsp45I 127 GTCAC ^GT[GC]AC
XmnI 292 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 11 enzymes) #
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AluI 317 AGCT AG^CT
527 AGCT
BsaJI 254 CCCAGG C^C[ACGT][ACGT]GG
255 CCAGGG
BsrI 490 ACTGG ACTGG(1/-1)
512 ACTGG
BstF5I 75 GGATG GGATG(2/0)
198 GGATG
EarI 22 CTCTTC CTCTTC(1/4)
440 CTCTTC
FokI 75 GGATG GGATG(9/13)
198 GGATG
Ksp632I 22 CTCTTC CTCTTC(1/4)
440 CTCTTC
MaeIII 127 GTCAC ^GT[ACGT]AC
446 GTAAC
MboII 23 TCTTC GAAGA(8/7)
441 TCTTC
MwoI 321 GCAGAAAATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
469 GCCACTTGTGC
TspRI 356 CAGTG CA[GC]TG[ACGT][ACGT]^
408 CAGTG
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# 3 OR MORE CUT ENZYMES ( 8 enzymes) #
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BbvI Fnu4HI HaeIII ItaI MnlI
SfaNI TseI Tsp509I