(Circular) (Six-base) MAPSORT of: pFN21AB8244.seq Check: 8208 from: 1 to: 5707 pFN21AB8244 5707 bp With 182 enzymes: SgfI * February 27, 2009 14:41 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3517 278 Size: 53 83 186 936 1981 2468 Fragments arranged by size: 2468 1981 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3050 1937 Size: 1113 4594 Acc65I G'GTAC_C Cuts at: 3028 3028 Size: 5707 AclI AA'CG_TT Cuts at: 1563 3834 4207 1563 Size: 2271 373 3063 Fragments arranged by size: 3063 2271 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2213 3770 5154 5422 1632 Size: 581 1557 1384 268 1917 Fragments arranged by size: 1917 1557 1384 581 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5510 AflIII A'CryG_T Cuts at: 1203 4606 5583 1203 Size: 3403 977 1327 Fragments arranged by size: 3403 1327 977 AgeI A'CCGG_T Cuts at: 4538 4538 Size: 5707 AhdI GACnn_n'nnGTC Cuts at: 4436 4436 Size: 5707 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5707 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5707 AlwNI CAG_nnn'CTG Cuts at: 3450 5022 3450 Size: 1572 4135 ApaLI G'TGCA_C Cuts at: 3764 4920 3764 Size: 1156 4551 ApoI r'AATT_y Cuts at: 1493 3016 3242 3394 5503 1493 Size: 1523 226 152 2109 1697 Fragments arranged by size: 2109 1697 1523 226 152 AseI AT'TA_AT Cuts at: 160 1032 4261 160 Size: 872 3229 1606 Fragments arranged by size: 3229 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5707 AvaI C'yCGr_G Cuts at: 1870 1943 2319 3032 1870 Size: 73 376 713 4545 Fragments arranged by size: 4545 713 376 73 BamHI G'GATC_C Cuts at: 3037 3404 3037 Size: 367 5340 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2555 3028 4483 618 Size: 325 217 627 768 473 1455 1842 Fragments arranged by size: 1842 1455 768 627 473 325 217 BanII G_rGCy'C Cuts at: 1391 3026 1391 Size: 1635 4072 BbeI G_GCGC'C Cuts at: 2559 2559 Size: 5707 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2901 928 Size: 971 1002 3734 Fragments arranged by size: 3734 1002 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3898 3932 1903 Size: 34 1961 34 3678 Fragments arranged by size: 3678 1961 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3599 4809 3599 Size: 1210 4497 BclI T'GATC_A Cuts at: 1548 2274 1548 Size: 726 4981 BfrBI ATG'CAT Cuts at: 2206 2206 Size: 5707 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2427 4318 Size: 107 122 71 631 605 337 418 1891 Cuts at: 4318 136 Size: 1525 Fragments arranged by size: 1891 1525 631 605 418 337 122 107 71 BglII A'GATC_T Cuts at: 5702 5702 Size: 5707 BlpI GC'TnA_GC Cuts at: 3124 3124 Size: 5707 Bme1580I G_kGCm'C Cuts at: 1790 2312 3768 4924 1790 Size: 522 1456 1156 2573 Fragments arranged by size: 2573 1456 1156 522 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5707 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2749 3438 4396 Size: 416 53 83 399 24 1332 689 958 Cuts at: 4396 442 Size: 1753 Fragments arranged by size: 1753 1332 958 689 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5707 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4367 1239 Size: 3128 2579 Bpu10I CC'TnA_GC Cuts at: 2331 2331 Size: 5707 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2738 3832 4697 4995 2738 Size: 1094 865 298 3450 Fragments arranged by size: 3450 1094 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4370 882 Size: 617 2871 2219 Fragments arranged by size: 2871 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3498 493 Size: 711 2294 2702 Fragments arranged by size: 2702 2294 711 BsaBI GATnn'nnATC Cuts at: 5701 5701 Size: 5707 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2012 2556 3514 3896 Size: 53 83 186 936 479 544 958 382 Cuts at: 3896 4531 275 Size: 635 1451 Fragments arranged by size: 1451 958 936 635 544 479 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4140 4538 4812 4959 5370 4140 Size: 398 274 147 411 4477 Fragments arranged by size: 4477 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5482 Fragments arranged by size: 5482 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5707 BseYI C'CCAG_C Cuts at: 2838 2842 4910 5406 2838 Size: 4 2068 496 3139 Fragments arranged by size: 3139 2068 496 4 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5134 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3157 3918 4946 1540 Size: 275 186 1156 761 1028 2301 Fragments arranged by size: 2301 1156 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3768 3853 4924 3768 Size: 85 1071 4551 Fragments arranged by size: 4551 1071 85 BsmI GAATG_Cn' Cuts at: 3218 3311 3218 Size: 93 5614 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2312 3026 3768 3853 4924 1391 Size: 399 522 714 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 714 522 399 85 BspEI T'CCGG_A Cuts at: 5370 5370 Size: 5707 BspHI T'CATG_A Cuts at: 3596 5326 3596 Size: 1730 3977 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3062 844 Size: 2218 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2022 3594 1394 Size: 429 199 1572 3507 Fragments arranged by size: 3507 1572 429 199 BsrDI GCAATG_nn' Cuts at: 65 1248 4202 4376 65 Size: 1183 2954 174 1396 Fragments arranged by size: 2954 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4351 4538 1700 Size: 110 2541 187 2869 Fragments arranged by size: 2869 2541 187 110 BsrGI T'GTAC_A Cuts at: 96 2650 96 Size: 2554 3153 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5707 BssSI C'ACGA_G Cuts at: 3767 4779 3767 Size: 1012 4695 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5707 BstBI TT'CG_AA Cuts at: 4511 4511 Size: 5707 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5707 BstYI r'GATC_y Cuts at: 1972 3037 3404 3789 3806 5258 5344 5356 5702 Size: 1065 367 385 17 1452 86 12 346 Cuts at: 5702 1972 Size: 1977 Fragments arranged by size: 1977 1452 1065 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2950 513 Size: 552 939 946 3270 Fragments arranged by size: 3270 946 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4968 Fragments arranged by size: 4968 683 56 BtsI GCAGTG_nn' Cuts at: 2511 2653 3223 4017 4037 2511 Size: 142 570 794 20 4181 Fragments arranged by size: 4181 794 570 142 20 ClaI AT'CG_AT Cuts at: 1554 2079 1554 Size: 525 5182 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5672 DraI TTT'AAA Cuts at: 3011 3011 Size: 5707 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4714 809 Size: 507 3398 1802 Fragments arranged by size: 3398 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2049 2386 3154 Size: 54 1425 50 275 18 219 337 768 Cuts at: 3154 4043 5672 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 768 337 275 219 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3154 1537 Size: 275 1342 4090 Fragments arranged by size: 4090 1342 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3637 1353 Size: 2284 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4294 4668 4814 387 Size: 1064 138 2705 374 146 1280 Fragments arranged by size: 2705 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2213 3770 4367 5154 5422 1239 Size: 393 581 1557 597 787 268 1524 Fragments arranged by size: 1557 1524 787 597 581 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2595 2595 Size: 5707 EcoO109I rG'GnC_Cy Cuts at: 2600 2600 Size: 5707 EcoRI G'AATT_C Cuts at: 3016 3394 3016 Size: 378 5329 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2567 2918 2567 Size: 351 5356 FspI TGC'GCA Cuts at: 4213 4213 Size: 5707 HaeII r_GCGC'y Cuts at: 1678 2559 4854 1678 Size: 881 2295 2531 Fragments arranged by size: 2531 2295 881 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2131 2163 2461 2493 2873 Size: 32 135 32 559 32 298 32 380 Cuts at: 2873 2905 4348 4380 4422 4454 1373 Size: 32 1443 32 42 32 2626 Fragments arranged by size: 2626 1443 559 380 298 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3051 3297 669 Size: 988 281 1113 246 3079 Fragments arranged by size: 3079 1113 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5707 HpaI GTT'AAC Cuts at: 3297 3297 Size: 5707 KasI G'GCGC_C Cuts at: 2555 2555 Size: 5707 KpnI G_GTAC'C Cuts at: 3032 3032 Size: 5707 MfeI C'AATT_G Cuts at: 3306 3306 Size: 5707 MluI A'CGCG_T Cuts at: 5583 5583 Size: 5707 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2543 4821 5005 1305 Size: 1238 2278 184 2007 Fragments arranged by size: 2278 2007 1238 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2051 10 Size: 54 1425 343 219 3666 Fragments arranged by size: 3666 1425 343 219 54 MslI CAynn'nnrTG Cuts at: 518 1166 3665 4024 4183 518 Size: 648 2499 359 159 2042 Fragments arranged by size: 2499 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2842 2925 3123 3800 4948 5193 1606 Size: 1236 83 198 677 1148 245 2120 Fragments arranged by size: 2120 1236 1148 677 245 198 83 NarI GG'CG_CC Cuts at: 2556 2556 Size: 5707 NcoI C'CATG_G Cuts at: 513 1065 2004 2950 513 Size: 552 939 946 3270 Fragments arranged by size: 3270 946 939 552 NdeI CA'TA_TG Cuts at: 387 2344 387 Size: 1957 3750 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5707 NotI GC'GGCC_GC Cuts at: 3154 3154 Size: 5707 NsiI A_TGCA'T Cuts at: 2208 2208 Size: 5707 NspI r_CATG'y Cuts at: 1132 1229 2062 2210 3065 4610 1132 Size: 97 833 148 855 1545 2229 Fragments arranged by size: 2229 1545 855 833 148 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5707 PciI A'CATG_T Cuts at: 4606 4606 Size: 5707 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5707 PfoI T'CCnGG_A Cuts at: 2015 2487 2015 Size: 472 5235 PmeI GTTT'AAAC Cuts at: 3011 3011 Size: 5707 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3776 3808 5289 5321 3776 Size: 32 1481 32 4162 Fragments arranged by size: 4162 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2600 2600 Size: 5707 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5707 PsiI TTA'TAA Cuts at: 3277 3277 Size: 5707 PstI C_TGCA'G Cuts at: 830 3059 830 Size: 2229 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5707 PvuII CAG'CTG Cuts at: 2842 2925 2842 Size: 83 5624 SalI G'TCGA_C Cuts at: 1936 3049 1936 Size: 1113 4594 SbfI CC_TGCA'GG Cuts at: 3059 3059 Size: 5707 ScaI AGT'ACT Cuts at: 2165 2464 3955 5667 2165 Size: 299 1491 1712 2205 Fragments arranged by size: 2205 1712 1491 299 SfcI C'TryA_G Cuts at: 826 1045 3055 4190 4871 5062 826 Size: 219 2010 1135 681 191 1471 Fragments arranged by size: 2010 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2557 2557 Size: 5707 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5707 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5707 SmaI CCC'GGG Cuts at: 1872 3034 1872 Size: 1162 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2717 3811 4712 4974 820 Size: 197 926 774 1094 901 262 1553 Fragments arranged by size: 1553 1094 926 901 774 262 197 SnaBI TAC'GTA Cuts at: 493 3498 493 Size: 3005 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5707 SphI G_CATG'C Cuts at: 1132 2210 3065 1132 Size: 1078 855 3774 Fragments arranged by size: 3774 1078 855 SspI AAT'ATT Cuts at: 5 52 2666 3631 5 Size: 47 2614 965 2081 Fragments arranged by size: 2614 2081 965 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5707 StyI C'CwwG_G Cuts at: 513 1065 2004 2950 3146 513 Size: 552 939 946 196 3074 Fragments arranged by size: 3074 946 939 552 196 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2731 2898 3734 3751 3904 4089 4534 Size: 1255 1151 167 836 17 153 185 445 Cuts at: 4534 325 Size: 1498 Fragments arranged by size: 1498 1255 1151 836 445 185 167 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2163 2462 2650 Size: 274 80 33 51 1443 186 299 188 Cuts at: 2650 3953 5665 96 Size: 1303 1712 138 Fragments arranged by size: 1712 1443 1303 299 274 188 186 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5667 5699 5667 Size: 32 5675 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5707 XbaI T'CTAG_A Cuts at: 3043 3043 Size: 5707 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2278 2429 2278 Size: 151 5556 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5707 XmaI C'CCGG_G Cuts at: 1870 3032 1870 Size: 1162 4545 XmnI GAAnn'nnTTC Cuts at: 3836 3836 Size: 5707 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3515 276 Size: 53 83 186 936 1981 2468 Fragments arranged by size: 2468 1981 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FseI FspAI NaeI NgoMIV NruI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI