Restriction map for
pFN21AB8242
(Linear) (Six-base) MAPSORT of: KIBB8242.seq Check: 7652 from: 1 to: 594
KIBB8242.orf
With 182 enzymes: SgfI *
March 3, 2008 11:04 ..
AccI GT'mk_AC
Cuts at: 0 359 594
Size: 359 235
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 164 594
Size: 164 430
ApoI r'AATT_y
Cuts at: 0 280 423 552 594
Size: 280 143 129 42
Fragments arranged by size:
280 143 129 42
AvaI C'yCGr_G
Cuts at: 0 9 594
Size: 9 585
BamHI G'GATC_C
Cuts at: 0 96 594
Size: 96 498
BanI G'GyrC_C
Cuts at: 0 22 594
Size: 22 572
BanII G_rGCy'C
Cuts at: 0 63 504 594
Size: 63 441 90
Fragments arranged by size:
441 90 63
BbeI G_GCGC'C
Cuts at: 0 26 594
Size: 26 568
BbsI GAAGACnn'nnnn_
Cuts at: 0 528 594
Size: 528 66
BciVI GTATCCnnnnn_n'
Cuts at: 0 190 522 594
Size: 190 332 72
Fragments arranged by size:
332 190 72
BlpI GC'TnA_GC
Cuts at: 0 536 594
Size: 536 58
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 212 235 333 594
Size: 212 23 98 261
Fragments arranged by size:
261 212 98 23
BsaHI Gr'CG_yC
Cuts at: 0 23 594
Size: 23 571
Bsp1286I G_dGCh'C
Cuts at: 0 63 504 594
Size: 63 441 90
Fragments arranged by size:
441 90 63
BspHI T'CATG_A
Cuts at: 0 584 594
Size: 584 10
BsrDI GCAATG_nn'
Cuts at: 0 185 594
Size: 185 409
BsrFI r'CCGG_y
Cuts at: 0 25 224 594
Size: 25 199 370
Fragments arranged by size:
370 199 25
BstBI TT'CG_AA
Cuts at: 0 519 594
Size: 519 75
BstYI r'GATC_y
Cuts at: 0 96 594
Size: 96 498
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 462 594
Size: 462 132
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 353 388 594
Size: 353 35 206
Fragments arranged by size:
353 206 35
DraI TTT'AAA
Cuts at: 0 285 594
Size: 285 309
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 212 235 333 594
Size: 212 23 98 261
Fragments arranged by size:
261 212 98 23
EcoO109I rG'GnC_Cy
Cuts at: 0 70 381 594
Size: 70 311 213
Fragments arranged by size:
311 213 70
EcoRI G'AATT_C
Cuts at: 0 423 594
Size: 423 171
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 480 594
Size: 480 114
HaeII r_GCGC'y
Cuts at: 0 26 594
Size: 26 568
KasI G'GCGC_C
Cuts at: 0 22 594
Size: 22 572
MfeI C'AATT_G
Cuts at: 0 428 594
Size: 428 166
MspA1I CmG'CkG
Cuts at: 0 102 594
Size: 102 492
NaeI GCC'GGC
Cuts at: 0 27 226 594
Size: 27 199 368
Fragments arranged by size:
368 199 27
NarI GG'CG_CC
Cuts at: 0 23 594
Size: 23 571
NdeI CA'TA_TG
Cuts at: 0 561 594
Size: 561 33
NgoMIV G'CCGG_C
Cuts at: 0 25 224 594
Size: 25 199 370
Fragments arranged by size:
370 199 25
PfoI T'CCnGG_A
Cuts at: 0 63 81 594
Size: 63 18 513
Fragments arranged by size:
513 63 18
SfcI C'TryA_G
Cuts at: 0 122 594
Size: 122 472
SfoI GGC'GCC
Cuts at: 0 24 594
Size: 24 570
SgrAI Cr'CCGG_yG
Cuts at: 0 25 594
Size: 25 569
SmaI CCC'GGG
Cuts at: 0 11 594
Size: 11 583
TatI w'GTAC_w
Cuts at: 0 300 594
Size: 300 294
Tth111I GACn'n_nGTC
Cuts at: 0 177 594
Size: 177 417
XmaI C'CCGG_G
Cuts at: 0 9 594
Size: 9 585
Enzymes that do cut:
AccI AlfI ApoI AvaI BamHI BanI BanII BbeI
BbsI BciVI BlpI BpmI BsaHI Bsp1286I BspHI BsrDI
BsrFI BstBI BstYI BtgZI CspCI DraI Eco57MI EcoO109I
EcoRI FalI HaeII KasI MfeI MspA1I NaeI NarI
NdeI NgoMIV PfoI SfcI SfoI SgrAI SmaI TatI
Tth111I XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AloI AlwNI ApaI ApaLI AscI
AseI AsiSI AvrII BaeI BbvCI BcgI BclI BfrBI
BglI BglII Bme1580I BmgBI BmrI BmtI BplI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI BspMI
BsrBI BsrGI BssHII BssSI BstAPI BstEII BstXI BstZ17I
Bsu36I BtgI BtsI ClaI DraIII DrdI EaeI EagI
EarI EciI EcoICRI EcoNI EcoRV FseI FspI FspAI
Hin4I HincII HindIII HpaI KpnI MluI MmeI MscI
MslI NcoI NheI NotI NruI NsiI NspI PacI
PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI PvuII RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SgfI SmlI SnaBI SpeI SphI SrfI SspI
StuI StyI SwaI TaqII TstI XbaI XcmI XhoI
XmnI ZraI