Restriction map for
pFN21AB8240
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# NO CUT ENZYMES ( 175 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BalI BamHI BanI BbeI
BbrPI BbvCI BcgI BciVI BcnI
BfaI BglI BglII BlnI BlpI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaJI BsaWI BseAI BsiEI
BsiWI BsmAI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FseI FspI Hin1I HindIII
HphI KasI KpnI Ksp632I KspI
MaeI MaeII MaeIII MamI MfeI
MflI MluI MluNI MroI MscI
MslI MspA1I MunI MvnI NarI
NciI NcoI NdeI NheI NlaIV
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsrII SacII SalI SapI SbfI
ScaI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI StyI SwaI
Tsp45I TspRI Tth111I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AciI 41 CCGC CCGC(-3/-1)
AfaI 307 GTAC GT^AC
AflII 457 CTTAAG C^TTAAG
AlwI 694 GATCC GGATC(4/5)
AlwNI 159 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 631 GAGCTC G[AT]GC[AT]^C
BanII 631 GAGCTC G[GA]GC[CT]^C
BclI 359 TGATCA T^GATCA
BfrI 457 CTTAAG C^TTAAG
BmyI 631 GAGCTC G[AGT]GC[ACT]^C
BseRI 628 GAGGAG GAGGAG(10/8)
BsgI 619 CTGCAC GTGCAG(16/14)
BsiHKAI 631 GAGCTC G[AT]GC[AT]^C
Bsp1286I 631 GAGCTC G[AGT]GC[ACT]^C
BspHI 435 TCATGA T^CATGA
BspMI 416 ACCTGC ACCTGC(4/8)
BsrFI 43 GCCGGC [GA]^CCGG[CT]
BssKI 447 CCTGG ^CC[ACGT]GG
BstAPI 682 GCAATTATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 447 CCTGG CC^[AT]GG
Cac8I 43 GCCGGC GC[ACGT]^[ACGT]GC
CfoI 486 GCGC GCG^C
Cfr10I 43 GCCGGC [GA]^CCGG[CT]
Cfr13I 167 GGCCC G^G[ACGT]CC
EcoNI 489 CCTGACACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII 447 CCTGG ^CC[AT]GG
FauI 40 CCCGC CCCGC(4/6)
FbaI 359 TGATCA T^GATCA
HaeII 485 AGCGCC [GA]GCGC^[CT]
HaeIII 167 GGCC GG^CC
HapII 44 CCGG C^CGG
HhaI 486 GCGC GCG^C
HinP1I 486 GCGC G^CGC
HpaI 199 GTTAAC GTT^AAC
HpaII 44 CCGG C^CGG
MspI 44 CCGG C^CGG
MvaI 447 CCTGG CC^[AT]GG
NaeI 43 GCCGGC GCC^GGC
NgoMIV 43 GCCGGC G^CCGGC
NlaIII 436 CATG CATG^
PflMI 708 CCACAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 435 TCATGA T^CATGA
RsaI 307 GTAC GT^AC
SacI 631 GAGCTC GAGCT^C
Sau96I 167 GGCCC G^G[ACGT]CC
ScrFI 447 CCTGG CC^[ACGT]GG
SexAI 446 ACCTGGT A^CC[AT]GGT
SfcI 643 CTACAG C^T[GA][CT]AG
SmlI 457 CTTAAG C^T[CT][GA]AG
Van91I 708 CCACAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 12 enzymes) #
###################################
BbsI 97 GAAGAC GAAGAC(2/6)
127 GAAGAC
BpuAI 97 GAAGAC GAAGAC(2/6)
127 GAAGAC
BsiYI 489 CCTGACACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
708 CCACAGACTGG
BslI 489 CCTGACACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
708 CCACAGACTGG
HgaI 8 GACGC GACGC(5/10)
432 GCGTC
HincII 15 GTTGAC GT[CT]^[GA]AC
199 GTTAAC
HindII 15 GTTGAC GT[CT]^[GA]AC
199 GTTAAC
HinfI 216 GATTC G^A[ACGT]TC
426 GATTC
SfaNI 499 GATGC GCATC(5/9)
580 GATGC
TaqI 26 TCGA T^CGA
225 TCGA
TfiI 216 GATTC G^A[AT]TC
426 GATTC
TthHB8I 26 TCGA T^CGA
225 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AcsI AluI ApoI BbvI BsrI
BstF5I DdeI DpnII Fnu4HI FokI
ItaI MboI MboII MnlI MseI
MwoI NdeII Sau3AI Tru9I TseI
Tsp509I