Restriction map for
pFN21AB8238
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AfaI AflII AflIII AgeI AhdI
AocI Aor51HI ApaI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BanI
BbeI BbrPI BbsI BbvCI BciVI
BfrI BglI BlnI BpuAI BsaI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspHI BspLU11I BsrDI BsrFI
BsrGI BssHII Bst1107I BstBI BstXI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoRI EcoRV EcoT14I EcoT22I FseI
FspI HaeII HindIII HpaI KasI
KpnI KspI MfeI MluI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI RsaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StyI SwaI Tth111I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccIII 134 TCCGGA T^CCGGA
AcyI 874 GGCGTC G[GA]^CG[CT]C
Alw44I 79 GTGCAC G^TGCAC
ApaLI 79 GTGCAC G^TGCAC
BalI 209 TGGCCA TGG^CCA
BcgI 579 GCAGGTGACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 710 TGATCA T^GATCA
BfaI 859 CTAG C^TAG
BglII 605 AGATCT A^GATCT
BlpI 740 GCTCAGC GC^T[ACGT]AGC
BpmI 787 CTGGAG CTGGAG(16/14)
Bpu1102I 740 GCTCAGC GC^T[ACGT]AGC
BsaAI 751 TACGTG [CT]AC^GT[GA]
BsaBI 825 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 874 GGCGTC G[GA]^CG[CT]C
BsaWI 134 TCCGGA [AT]^CCGG[AT]
BseAI 134 TCCGGA T^CCGGA
BsgI 658 CTGCAC GTGCAG(16/14)
BsmAI 24 GAGAC GTCTC(1/5)
BspEI 134 TCCGGA T^CCGGA
BsrI 162 ACTGG ACTGG(1/-1)
BssSI 16 CACGAG CACGAG(-5/-1)
BstEII 198 GGTCACC G^GT[ACGT]ACC
BstPI 198 GGTCACC G^GT[ACGT]ACC
Cac8I 293 GCCTGC GC[ACGT]^[ACGT]GC
CelII 740 GCTCAGC GC^T[ACGT]AGC
EaeI 209 TGGCCA [CT]^GGCC[GA]
EarI 49 GAAGAG CTCTTC(1/4)
EcoNI 219 CCTTCCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 198 GGTCACC G^GT[ACGT]ACC
FbaI 710 TGATCA T^GATCA
HgaI 875 GCGTC GACGC(5/10)
Hin1I 874 GGCGTC G[GA]^CG[CT]C
HincII 352 GTCAAC GT[CT]^[GA]AC
HindII 352 GTCAAC GT[CT]^[GA]AC
Ksp632I 49 GAAGAG CTCTTC(1/4)
MaeI 859 CTAG C^TAG
MamI 825 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 209 TGGCCA TGG^CCA
MroI 134 TCCGGA T^CCGGA
MscI 209 TGGCCA TGG^CCA
MseI 598 TTAA T^TAA
PflMI 177 CCAGGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 585 GACTC GAGTC(4/5)
PshAI 150 GACTATTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SmaI 84 CCCGGG CCC^GGG
StuI 593 AGGCCT AGG^CCT
TaqI 588 TCGA T^CGA
TfiI 465 GATTC G^A[AT]TC
Tru9I 598 TTAA T^TAA
TthHB8I 588 TCGA T^CGA
Van91I 177 CCAGGAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 488 CCAATGCTGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 84 CCCGGG C^CCGGG
XmaI 84 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccII 69 CGCG CG^CG
259 CGCG
AlwI 288 GATCC GGATC(4/5)
627 GATCC
AvaI 84 CCCGGG C^[CT]CG[GA]G
223 CCCGAG
BsmFI 5 GGGAC GGGAC(10/14)
390 GTCCC
BsoBI 84 CCCGGG C^[CT]CG[GA]G
223 CCCGAG
BsrBI 61 GAGCGG CCGCTC(-3/-3)
460 GAGCGG
BstAPI 240 GCAGGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
249 GCAGAACCTGC
BstUI 69 CGCG CG^CG
259 CGCG
CfoI 70 GCGC GCG^C
258 GCGC
DraII 10 AGGACCC [GA]G^G[ACGT]CC[CT]
383 GGGTCCT
EcoO109I 10 AGGACCC [GA]G^G[ACGT]CC[CT]
383 GGGTCCT
FauI 3 GCGGG CCCGC(4/6)
63 GCGGG
HhaI 70 GCGC GCG^C
258 GCGC
HinP1I 70 GCGC G^CGC
258 GCGC
HinfI 465 GATTC G^A[ACGT]TC
585 GACTC
MvnI 69 CGCG CG^CG
259 CGCG
NlaIII 234 CATG CATG^
379 CATG
PpuMI 10 AGGACCC [GA]G^G[AT]CC[CT]
383 GGGTCCT
PstI 247 CTGCAG CTGCA^G
295 CTGCAG
SacI 406 GAGCTC GAGCT^C
738 GAGCTC
SfaNI 525 GATGC GCATC(5/9)
696 GATGC
SfcI 247 CTGCAG C^T[GA][CT]AG
295 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AluI AlwNI AspHI AvaII
BanII BbvI BcnI BmyI BsaJI
BseRI BsiHKAI BsiYI BslI Bsp1286I
BspMI BssKI BstF5I BstNI BstYI
Cfr13I DdeI DpnII EcoRII Fnu4HI
FokI HaeIII HapII HpaII HphI
ItaI MaeII MaeIII MboI MboII
MflI MnlI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIV
PvuII Sau3AI Sau96I ScrFI TseI
Tsp45I Tsp509I TspRI XhoII