(Circular) (Six-base) MAPSORT of: pFN21AB8238.seq Check: 3128 from: 1 to: 5596 pFN21AB8238 5596 bp With 182 enzymes: SgfI * February 27, 2009 14:41 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2575 2575 Size: 5596 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3406 278 Size: 53 83 186 936 1870 2468 Fragments arranged by size: 2468 1870 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2939 1937 Size: 1002 4594 Acc65I G'GTAC_C Cuts at: 2917 2917 Size: 5596 AclI AA'CG_TT Cuts at: 1563 3723 4096 1563 Size: 2160 373 3063 Fragments arranged by size: 3063 2160 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2327 2504 2594 3659 5043 5311 1632 Size: 695 177 90 1065 1384 268 1917 Fragments arranged by size: 1917 1384 1065 695 268 177 90 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5399 AflIII A'CryG_T Cuts at: 1203 4495 5472 1203 Size: 3292 977 1327 Fragments arranged by size: 3292 1327 977 AgeI A'CCGG_T Cuts at: 4427 4427 Size: 5596 AhdI GACnn_n'nnGTC Cuts at: 4325 4325 Size: 5596 AleI CACnn'nnGTG Cuts at: 1166 2585 1166 Size: 1419 4177 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2450 1184 Size: 1266 4330 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2108 2140 2336 2368 2108 Size: 32 196 32 5336 Fragments arranged by size: 5336 196 32 32 AlwNI CAG_nnn'CTG Cuts at: 2251 2260 2533 3339 4911 2251 Size: 9 273 806 1572 2936 Fragments arranged by size: 2936 1572 806 273 9 ApaLI G'TGCA_C Cuts at: 2084 3653 4809 2084 Size: 1569 1156 2871 Fragments arranged by size: 2871 1569 1156 ApoI r'AATT_y Cuts at: 1493 2905 3131 3283 5392 1493 Size: 1412 226 152 2109 1697 Fragments arranged by size: 2109 1697 1412 226 152 AseI AT'TA_AT Cuts at: 160 1032 4150 160 Size: 872 3118 1606 Fragments arranged by size: 3118 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5596 AvaI C'yCGr_G Cuts at: 1870 1943 2089 2228 2921 1870 Size: 73 146 139 693 4545 Fragments arranged by size: 4545 693 146 139 73 BamHI G'GATC_C Cuts at: 2926 3293 2926 Size: 367 5229 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2917 4372 618 Size: 325 217 627 1130 1455 1842 Fragments arranged by size: 1842 1455 1130 627 325 217 BanII G_rGCy'C Cuts at: 1391 2114 2322 2415 2747 2915 1391 Size: 723 208 93 332 168 4072 Fragments arranged by size: 4072 723 332 208 168 93 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4625 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 2573 2607 3787 3821 1903 Size: 34 50 34 552 34 1180 34 3678 Fragments arranged by size: 3678 1180 552 50 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3488 4698 3488 Size: 1210 4386 BclI T'GATC_A Cuts at: 1548 2715 1548 Size: 1167 4429 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4207 136 Size: 107 122 71 631 605 2535 1525 Fragments arranged by size: 2535 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2610 5591 2610 Size: 2981 2615 BlpI GC'TnA_GC Cuts at: 2746 3013 2746 Size: 267 5329 Bme1580I G_kGCm'C Cuts at: 1790 2088 2096 3657 4813 1790 Size: 298 8 1561 1156 2573 Fragments arranged by size: 2573 1561 1156 298 8 BmgBI CAC'GTC Cuts at: 1318 2779 1318 Size: 1461 4135 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2177 3327 4285 Size: 416 53 83 399 24 760 1150 958 Cuts at: 4285 442 Size: 1753 Fragments arranged by size: 1753 1150 958 760 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5596 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2813 4256 1239 Size: 1574 1443 2579 Fragments arranged by size: 2579 1574 1443 Bpu10I CC'TnA_GC Cuts at: 2741 2741 Size: 5596 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3721 4586 4884 3721 Size: 865 298 4433 Fragments arranged by size: 4433 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4259 882 Size: 617 2760 2219 Fragments arranged by size: 2760 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2758 3387 493 Size: 711 1554 629 2702 Fragments arranged by size: 2702 1554 711 629 BsaBI GATnn'nnATC Cuts at: 2834 5590 2834 Size: 2756 2840 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2880 3403 3785 4420 Size: 53 83 186 936 1347 523 382 635 Cuts at: 4420 275 Size: 1451 Fragments arranged by size: 1451 1347 936 635 523 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2139 4029 4427 4701 4848 5259 2139 Size: 1890 398 274 147 411 2476 Fragments arranged by size: 2476 1890 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2336 2366 1178 Size: 30 165 30 933 30 4408 Fragments arranged by size: 4408 933 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2135 2246 2531 1186 Size: 949 111 285 4251 Fragments arranged by size: 4251 949 285 111 BseYI C'CCAG_C Cuts at: 2104 2281 2379 2397 2498 4799 5295 2104 Size: 177 98 18 101 2301 496 2405 Fragments arranged by size: 2405 2301 496 177 101 98 18 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2648 1163 Size: 573 912 4111 Fragments arranged by size: 4111 912 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3046 3807 4835 1540 Size: 275 186 1045 761 1028 2301 Fragments arranged by size: 2301 1045 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2088 2415 2747 2778 3657 3742 4813 2088 Size: 327 332 31 879 85 1071 2871 Fragments arranged by size: 2871 1071 879 332 327 85 31 BsmI GAATG_Cn' Cuts at: 3107 3200 3107 Size: 93 5503 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2088 2096 2114 2322 2415 2747 2778 Size: 399 298 8 18 208 93 332 31 Cuts at: 2778 2915 3657 3742 4813 1391 Size: 137 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 332 298 208 137 93 85 31 18 8 BspEI T'CCGG_A Cuts at: 2139 5259 2139 Size: 3120 2476 BspHI T'CATG_A Cuts at: 3485 5215 3485 Size: 1730 3866 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2191 2268 2575 2951 844 Size: 1347 77 307 376 3489 Fragments arranged by size: 3489 1347 376 307 77 BsrBI CCG'CTC Cuts at: 1394 1823 2068 2467 3483 1394 Size: 429 245 399 1016 3507 Fragments arranged by size: 3507 1016 429 399 245 BsrDI GCAATG_nn' Cuts at: 65 1248 4091 4265 65 Size: 1183 2843 174 1396 Fragments arranged by size: 2843 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4240 4427 1700 Size: 110 2430 187 2869 Fragments arranged by size: 2869 2430 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5596 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5596 BssSI C'ACGA_G Cuts at: 2021 3656 4668 2021 Size: 1635 1012 2949 Fragments arranged by size: 2949 1635 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2251 2260 794 Size: 1457 9 4130 Fragments arranged by size: 4130 1457 9 BstBI TT'CG_AA Cuts at: 4400 4400 Size: 5596 BstEII G'GTnAC_C Cuts at: 1184 2203 1184 Size: 1019 4577 BstYI r'GATC_y Cuts at: 1972 2292 2610 2631 2926 3293 3678 3695 5147 Size: 320 318 21 295 367 385 17 1452 Cuts at: 5147 5233 5245 5591 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 320 318 295 86 21 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5044 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2280 487 Size: 683 56 1054 3803 Fragments arranged by size: 3803 1054 683 56 BtsI GCAGTG_nn' Cuts at: 3112 3906 3926 3112 Size: 794 20 4782 Fragments arranged by size: 4782 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5596 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5561 DraI TTT'AAA Cuts at: 2900 2900 Size: 5596 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4603 809 Size: 507 3287 1802 Fragments arranged by size: 3287 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2214 3043 3932 Size: 54 1425 50 275 18 384 829 889 Cuts at: 3932 5561 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 829 384 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3043 1537 Size: 275 1231 4090 Fragments arranged by size: 4090 1231 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2049 3526 1353 Size: 696 1477 3423 Fragments arranged by size: 3423 1477 696 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2285 4183 4557 4703 387 Size: 1064 138 696 1898 374 146 1280 Fragments arranged by size: 1898 1280 1064 696 374 146 138 EcoICRI GAG'CTC Cuts at: 2413 2745 2413 Size: 332 5264 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2327 2504 2594 2813 3659 4256 5043 Size: 393 695 177 90 219 846 597 787 Cuts at: 5043 5311 1239 Size: 268 1524 Fragments arranged by size: 1524 846 787 695 597 393 268 219 177 90 EcoNI CCTnn'n_nnAGG Cuts at: 2228 2228 Size: 5596 EcoO109I rG'GnC_Cy Cuts at: 2016 2389 2016 Size: 373 5223 EcoRI G'AATT_C Cuts at: 2905 3283 2905 Size: 378 5218 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2842 2842 Size: 5596 FspI TGC'GCA Cuts at: 4102 4102 Size: 5596 HaeII r_GCGC'y Cuts at: 1678 4743 1678 Size: 3065 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2030 2062 2704 2736 4237 Size: 32 135 32 458 32 642 32 1501 Cuts at: 4237 4269 4311 4343 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1501 642 458 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2359 2940 3186 669 Size: 988 281 421 581 246 3079 Fragments arranged by size: 3079 988 581 421 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5596 HpaI GTT'AAC Cuts at: 3186 3186 Size: 5596 KpnI G_GTAC'C Cuts at: 2921 2921 Size: 5596 MfeI C'AATT_G Cuts at: 3195 3195 Size: 5596 MluI A'CGCG_T Cuts at: 5472 5472 Size: 5596 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2169 2502 2872 4710 4894 1305 Size: 864 333 370 1838 184 2007 Fragments arranged by size: 2007 1838 864 370 333 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2216 10 Size: 54 1425 343 384 3390 Fragments arranged by size: 3390 1425 384 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2366 2585 2840 3554 3913 4072 518 Size: 648 1200 219 255 714 359 159 2042 Fragments arranged by size: 2042 1200 714 648 359 255 219 159 MspA1I CmG'CkG Cuts at: 1606 2281 2305 2482 2750 3012 3689 4837 5082 Size: 675 24 177 268 262 677 1148 245 Cuts at: 5082 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 675 268 262 245 177 24 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5044 NdeI CA'TA_TG Cuts at: 387 387 Size: 5596 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5596 NotI GC'GGCC_GC Cuts at: 3043 3043 Size: 5596 NspI r_CATG'y Cuts at: 1132 1229 2954 4499 1132 Size: 97 1725 1545 2229 Fragments arranged by size: 2229 1725 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5596 PciI A'CATG_T Cuts at: 4495 4495 Size: 5596 PflMI CCAn_nnn'nTGG Cuts at: 1108 2188 1108 Size: 1080 4516 PfoI T'CCnGG_A Cuts at: 2181 2181 Size: 5596 PmeI GTTT'AAAC Cuts at: 2900 2900 Size: 5596 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2336 2368 3665 3697 5178 5210 2336 Size: 32 1297 32 1481 32 2722 Fragments arranged by size: 2722 1481 1297 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2016 2389 2016 Size: 373 5223 PshAI GACnn'nnGTC Cuts at: 1613 2159 1613 Size: 546 5050 PsiI TTA'TAA Cuts at: 3166 3166 Size: 5596 PstI C_TGCA'G Cuts at: 830 2256 2304 2948 830 Size: 1426 48 644 3478 Fragments arranged by size: 3478 1426 644 48 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5596 PvuII CAG'CTG Cuts at: 2281 2305 2482 2281 Size: 24 177 5395 Fragments arranged by size: 5395 177 24 SacI G_AGCT'C Cuts at: 2415 2747 2415 Size: 332 5264 SalI G'TCGA_C Cuts at: 1936 2938 1936 Size: 1002 4594 SbfI CC_TGCA'GG Cuts at: 2948 2948 Size: 5596 ScaI AGT'ACT Cuts at: 3844 5556 3844 Size: 1712 3884 SfcI C'TryA_G Cuts at: 826 1045 2252 2300 2944 4079 4760 4951 826 Size: 219 1207 48 644 1135 681 191 1471 Fragments arranged by size: 1471 1207 1135 681 644 219 191 48 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5596 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5596 SmaI CCC'GGG Cuts at: 1872 2091 2923 1872 Size: 219 832 4545 Fragments arranged by size: 4545 832 219 SmlI C'TyrA_G Cuts at: 820 1017 1943 3700 4601 4863 820 Size: 197 926 1757 901 262 1553 Fragments arranged by size: 1757 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3387 493 Size: 2894 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5596 SphI G_CATG'C Cuts at: 1132 2954 1132 Size: 1822 3774 SspI AAT'ATT Cuts at: 5 52 3520 5 Size: 47 3468 2081 Fragments arranged by size: 3468 2081 47 StuI AGG'CCT Cuts at: 1517 2600 1517 Size: 1083 4513 StyI C'CwwG_G Cuts at: 513 1065 3035 513 Size: 552 1970 3074 Fragments arranged by size: 3074 1970 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3623 3640 3793 3978 4423 325 Size: 1255 2043 17 153 185 445 1498 Fragments arranged by size: 2043 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3842 5554 96 Size: 274 80 33 51 1443 1865 1712 138 Fragments arranged by size: 1865 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2209 2241 5556 5588 2209 Size: 32 3315 32 2217 Fragments arranged by size: 3315 2217 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5596 XbaI T'CTAG_A Cuts at: 2932 2932 Size: 5596 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2500 2500 Size: 5596 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5596 XmaI C'CCGG_G Cuts at: 1870 2089 2921 1870 Size: 219 832 4545 Fragments arranged by size: 4545 832 219 XmnI GAAnn'nnTTC Cuts at: 3725 3725 Size: 5596 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3404 276 Size: 53 83 186 936 1870 2468 Fragments arranged by size: 2468 1870 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI