Restriction map for
pFN21AB8238
(Linear) (Six-base) MAPSORT of: KIBB8238.seq Check: 83 from: 1 to: 891
KIBB8238.orf
With 182 enzymes: SgfI *
March 3, 2008 11:04 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 570 891
Size: 570 321
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 499 589 891
Size: 322 177 90 302
Fragments arranged by size:
322 302 177 90
AleI CACnn'nnGTG
Cuts at: 0 580 891
Size: 580 311
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 445 891
Size: 445 446
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 103 135 331 363 891
Size: 103 32 196 32 528
Fragments arranged by size:
528 196 103 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 246 255 528 891
Size: 246 9 273 363
Fragments arranged by size:
363 273 246 9
ApaLI G'TGCA_C
Cuts at: 0 79 891
Size: 79 812
AvaI C'yCGr_G
Cuts at: 0 84 223 891
Size: 84 139 668
Fragments arranged by size:
668 139 84
BanII G_rGCy'C
Cuts at: 0 109 317 410 742 891
Size: 109 208 93 332 149
Fragments arranged by size:
332 208 149 109 93
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 568 602 891
Size: 568 34 289
Fragments arranged by size:
568 289 34
BclI T'GATC_A
Cuts at: 0 710 891
Size: 710 181
BglII A'GATC_T
Cuts at: 0 605 891
Size: 605 286
BlpI GC'TnA_GC
Cuts at: 0 741 891
Size: 741 150
Bme1580I G_kGCm'C
Cuts at: 0 83 91 891
Size: 83 8 800
Fragments arranged by size:
800 83 8
BmgBI CAC'GTC
Cuts at: 0 774 891
Size: 774 117
BmrI ACTGGGnnnn_n'
Cuts at: 0 172 891
Size: 172 719
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 808 891
Size: 808 83
Bpu10I CC'TnA_GC
Cuts at: 0 736 891
Size: 736 155
BsaAI yAC'GTr
Cuts at: 0 753 891
Size: 753 138
BsaBI GATnn'nnATC
Cuts at: 0 829 891
Size: 829 62
BsaHI Gr'CG_yC
Cuts at: 0 875 891
Size: 875 16
BsaWI w'CCGG_w
Cuts at: 0 134 891
Size: 134 757
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 331 361 891
Size: 331 30 530
Fragments arranged by size:
530 331 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 130 241 526 891
Size: 130 111 285 365
Fragments arranged by size:
365 285 130 111
BseYI C'CCAG_C
Cuts at: 0 99 276 374 392 493 891
Size: 99 177 98 18 101 398
Fragments arranged by size:
398 177 101 99 98 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 643 891
Size: 643 248
BsiHKAI G_wGCw'C
Cuts at: 0 83 410 742 773 891
Size: 83 327 332 31 118
Fragments arranged by size:
332 327 118 83 31
Bsp1286I G_dGCh'C
Cuts at: 0 83 91 109 317 410 742 773 891
Size: 83 8 18 208 93 332 31 118
Fragments arranged by size:
332 208 118 93 83 31 18 8
BspEI T'CCGG_A
Cuts at: 0 134 891
Size: 134 757
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 186 263 570 891
Size: 186 77 307 321
Fragments arranged by size:
321 307 186 77
BsrBI CCG'CTC
Cuts at: 0 63 462 891
Size: 63 399 429
Fragments arranged by size:
429 399 63
BssSI C'ACGA_G
Cuts at: 0 16 891
Size: 16 875
BstAPI GCAn_nnn'nTGC
Cuts at: 0 246 255 891
Size: 246 9 636
Fragments arranged by size:
636 246 9
BstEII G'GTnAC_C
Cuts at: 0 198 891
Size: 198 693
BstYI r'GATC_y
Cuts at: 0 287 605 626 891
Size: 287 318 21 265
Fragments arranged by size:
318 287 265 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 275 891
Size: 275 616
EaeI y'GGCC_r
Cuts at: 0 209 891
Size: 209 682
EarI CTCTTCn'nnn_
Cuts at: 0 44 891
Size: 44 847
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 280 891
Size: 280 611
EcoICRI GAG'CTC
Cuts at: 0 408 740 891
Size: 408 332 151
Fragments arranged by size:
408 332 151
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 322 499 589 808 891
Size: 322 177 90 219 83
Fragments arranged by size:
322 219 177 90 83
EcoNI CCTnn'n_nnAGG
Cuts at: 0 223 891
Size: 223 668
EcoO109I rG'GnC_Cy
Cuts at: 0 11 384 891
Size: 11 373 507
Fragments arranged by size:
507 373 11
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 837 891
Size: 837 54
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 25 57 699 731 891
Size: 25 32 642 32 160
Fragments arranged by size:
642 160 32 32 25
HincII GTy'rAC
Cuts at: 0 354 891
Size: 354 537
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 164 497 867 891
Size: 164 333 370 24
Fragments arranged by size:
370 333 164 24
MscI TGG'CCA
Cuts at: 0 211 891
Size: 211 680
MslI CAynn'nnrTG
Cuts at: 0 361 580 835 891
Size: 361 219 255 56
Fragments arranged by size:
361 255 219 56
MspA1I CmG'CkG
Cuts at: 0 276 300 477 745 891
Size: 276 24 177 268 146
Fragments arranged by size:
276 268 177 146 24
PflMI CCAn_nnn'nTGG
Cuts at: 0 183 891
Size: 183 708
PfoI T'CCnGG_A
Cuts at: 0 176 891
Size: 176 715
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 331 363 891
Size: 331 32 528
Fragments arranged by size:
528 331 32
PpuMI rG'GwC_Cy
Cuts at: 0 11 384 891
Size: 11 373 507
Fragments arranged by size:
507 373 11
PshAI GACnn'nnGTC
Cuts at: 0 154 891
Size: 154 737
PstI C_TGCA'G
Cuts at: 0 251 299 891
Size: 251 48 592
Fragments arranged by size:
592 251 48
PvuII CAG'CTG
Cuts at: 0 276 300 477 891
Size: 276 24 177 414
Fragments arranged by size:
414 276 177 24
SacI G_AGCT'C
Cuts at: 0 410 742 891
Size: 410 332 149
Fragments arranged by size:
410 332 149
SfcI C'TryA_G
Cuts at: 0 247 295 891
Size: 247 48 596
Fragments arranged by size:
596 247 48
SmaI CCC'GGG
Cuts at: 0 86 891
Size: 86 805
StuI AGG'CCT
Cuts at: 0 595 891
Size: 595 296
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 204 236 891
Size: 204 32 655
Fragments arranged by size:
655 204 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 495 891
Size: 495 396
XmaI C'CCGG_G
Cuts at: 0 84 891
Size: 84 807
Enzymes that do cut:
AarI AcuI AleI AlfI AloI AlwNI ApaLI AvaI
BanII BcgI BclI BglII BlpI Bme1580I BmgBI BmrI
BpmI Bpu10I BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiHKAI Bsp1286I BspEI BspMI BsrBI BssSI
BstAPI BstEII BstYI BtgZI EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FalI Hin4I HincII MmeI MscI
MslI MspA1I PflMI PfoI PpiI PpuMI PshAI PstI
PvuII SacI SfcI SmaI StuI TstI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI ApaI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BanI BbeI BbsI BbvCI BciVI BfrBI BglI
BmtI BplI BpuEI BsaI BsiEI BsiWI BsmI BsmBI
BspHI BsrDI BsrFI BsrGI BssHII BstBI BstXI BstZ17I
Bsu36I BtgI BtsI ClaI CspCI DraI DraIII DrdI
EagI EcoRI EcoRV FseI FspI FspAI HaeII HindIII
HpaI KasI KpnI MfeI MluI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PmeI PmlI PsiI PspOMI PspXI PsrI
PvuI RsrII SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI SspI StyI SwaI TaqII TatI Tth111I
XbaI XhoI XmnI ZraI